Goat uterine epithelial cells are susceptible to infection with Caprine Arthritis Encephalitis Virus (CAEV) in vivo

ABSTRACT: The aim of this study was to determine, using immunofluorescence and in situ hybridization, whether CAEV is capable of infecting goat uterine epithelial cells in vivo. Five CAEV seropositive goats confirmed as infected using double nested polymerase chain reaction (dnPCR) on leucocytes and on vaginal secretions were used as CAEV positive goats. Five CAEV-free goats were used as controls. Samples from the uterine horn were prepared for dnPCR, in situ hybridization, and immunofluorescence. The results from dnPCR confirmed the presence of CAEV proviral DNA in the uterine horn samples of infected goats whereas no CAEV proviral DNA was detected in samples taken from the uninfected control goats. The in situ hybridization probe was complementary to part of the CAEV gag gene and confirmed the presence of CAEV nucleic acids in uterine samples. The positively staining cells were seen concentrated in the mucosa of the lamina propria of uterine sections. Finally, laser confocal analysis of double p28/cytokeratin immunolabelled transverse sections of CAEV infected goat uterus, demonstrated that the virus was localized in glandular and epithelial cells. This study clearly demonstrates that goat uterine epithelial cells are susceptible to CAEV infection in vivo. This finding could help to further our understanding of the epidemiology of CAEV, and in particular the possibility of vertical transmission.     

生态系统方法在太湖渔业管理中的应用

在综述生态系统方法的相关理论和实地考察的基础上,结合太湖生态系统脆弱性特点和太湖渔业资源开发利用的实际情况,揭示了当前太湖渔业管理中存在的问题。主要包括:渔业环境、渔业的结构性矛盾、渔业管理体制、渔业管理辅助手段四个方面。从而提出在太湖渔业管理中运用生态系统方法的实施步骤。在明确生态系统边界,确立生态系统管理目标的基础上,对太湖渔业管理体制进行改革,将管理机构划分为三个系统:管理、执行和监测系统,从而实现权责统一制的基于生态系统的渔业管理体系。同时,也提出加快渔业法制建设、壮大渔业管理人才队伍、提高执法人员整体素质、完善相应配套的渔业管理辅助措施以及广泛加强对渔业管理中生态系统方法理念的宣传教育等一系列措施。     

钝顶螺旋藻类囊体膜的叶绿素蛋白复合物的基本特性

钝顶螺旋藻 Spirulina platensis 类囊体膜经提取后,用 SDS-PAGE 分离得三条叶绿素蛋白复合物区带,按迁移率顺序,它们分别是 CPI,CPa 和游离色素带 FC。CPI 在红区和兰区的吸收峰分别为678nm 和441nm,CPa 在红区和兰区的吸收峰分别为676nm 和440nm。室温下 CPI 荧光发射峰位于686nm,而 CPa 荧光发射峰位于684nm。CPI 分子量为87 000,CPa分子量为49 500。测定了 CPI 的氨基酸组成。CPI 远紫外圆二色谱测定计算结果,CPI 的二级结构中α-螺旋含量为35.8%,β-折叠为19.9%,无规卷曲为44.2%。     

小球藻类囊体叶绿素蛋白复合物的基本特征(简报)

小球藻属绿藻门,为早期研究光合作用常用的材料。因其藻体结构简单,又具完整的光合器官,故至今仍为研究光合作用的重要材料。本试验测定了小球藻类囊体膜上的叶绿素蛋白复合物的基本特性,试探讨其在光合作用中的功能并与兰藻(螺旋藻)进行比较,为阐明绿藻在植物进化过程中的地位提供理论依据。试验材料为小球藻(Chlorella pyrenoidosa)于培养液中置30℃恒温箱,昼夜光照     

Insights into the spatio-temporal variability of spawning in a territorial coastal fish by combining observations,modelling and literature review

In bipartite life cycle fishes, spawning represents the onset of propagules dispersal, with eggs and larvae experiencing anisotropic transport and high mortality rates, before eventually metamorphosing and settling. Hence, early-life stages operate as bottlenecks for population demography by strongly constraining recruitment. Despite its significance, spawning is rarely explicitly considered in ecosystem management due to a lack of knowledge, for many species, about where and when spawning occurs. Previous evidences suggest that temperature is among the main drivers of spawning in Teleosts. Using the ecologically and economically relevant white seabream Diplodus sargus in the central Mediterranean Sea as a case study, we assess the abiotic factors that regulate the onset and duration of spawning and subsequent dispersal. Lagrangian backtracking simulations fed with early-life observations allow locating 11 spawning events, which are then associated to simulated temperatures ranging from 14.8° C to 20.6° C, in close agreement with previous estimates. Based on this range of suitable temperatures, we model the spatio-temporal variability of spawning success at broad-scale over 10 years (2005–2014) following the backtracking approach with hypothetical constant settlement areas. It highlights a prominent inter-annual variability in the Adriatic and Siculo-Tunisian strait driven by oceanographic processes. Moreover, a powerful clustering method uncovers relatively stable spawning areas in the Ligurian and Tyrrhenian seas with both early (January to Mid-February) and late (April to June) spawning peaks. Our methodology can be applied to other species and oceanic systems to investigate how oceanic processes impact spawning success, enabling the design of sound management strategies.     

Biological investigations of anchovy and some selected fish species caught during the R／V “Bei Dou”surveys 1984—1999

IntroductionProductivity of an ecosystem may be measured in terms of structure,abundance and growth of keyspecies.Stock structure,measured as age or size composition in a given stock may indicate whether the stockis in a state of growing,stable or declining.Individual growth may be measured in terms of annual growth ofindividual age groups and maturity of key species will similarly reflect the potential of the species to copewith the influence of a changing environment of predators and human i…     

长江口及邻近水域渔业环境质量的现状及变化趋势研究

本文根据2000～2002年6个航次的调查资料,对长江口及邻近水域的渔业环境质量进行全面研究及变动趋势分析。研究结果表明,长江口及邻近水域营养盐含量十分丰富,约90％无机氮是以硝酸盐形态存在。该水域主要受营养盐、重金属及有机物的污染。其中杭州湾水域受污染程度最为严重。长江口及邻近水域浮游植物初级生产主要受磷营养盐的限制。总的来说,长江口及邻近水域渔业环境质量正呈逐步下降趋势。     

江豚的形态特征和亚种划分问题

本文通过对各海域江豚主要形态特征性状差异显著比较,讨论了江豚亚种划分问题。作者认为江豚包含3个亚种:南海种群与印度,巴基斯坦种群同属指名亚种Neophocaena phocaenoides phocaenoides,东海一长江种群属杨子亚种N.p.asiaeorientalis,黄渤海种群与日本种群均属北方亚种N.p.sunameri。     

洋山工程海域虾蟹类资源的初步分析

根据2003年-2005年每年5月、8月和11月对洋山深水港一期工程施工海域(30°30′～30°54,N、121°45′～122°30′E)20个站点进行渔业资源监测调查,分析了该海域虾蟹类资源的种类组成、生物量、丰度、生物多样性、个体平均体重及幼体比例等的变化,并与工程前(2001年)相比较。结果显示:虾蟹类的种类基本逐年减少;3年平均的生物量和丰度比工程前低;多样性指数、平均个体体重和幼体比例也是呈逐年降低的趋势。这系列的变化反映出该海域渔业资源结构的改变朝小型化方向发展,群落结构趋于简单化,容易引起生态系统结构与功能的改变。说明洋山工程海域施工期对虾蟹类资源产生了一定程度的影响。