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11.
A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. 总被引:61,自引:0,他引:61
The v-akt oncogene codes for a 105-kilodalton fusion phosphoprotein containing Gag sequences at its amino terminus. Sequence analysis of v-akt and biochemical characterization of its product revealed that it codes for a protein kinase C-related serine-threonine kinase whose cellular homolog is expressed in most tissues, with the highest amount found in thymus. Although Akt is a serine-threonine kinase, part of its regulatory region is similar to the Src homology-2 domain, a structural motif characteristic of cytoplasmic tyrosine kinases that functions in protein-protein interactions. This suggests that Akt may form a functional link between tyrosine and serine-threonine phosphorylation pathways. 相似文献
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Summary The resistance of ten potato cultivars (Agria, Ajax, Désirée, Liseta, Kennebec, Majestic. Monalisa, Prima, Spunta and Tonda
di Berlino) toPhytophthora infestans was analyzed in vitro using 8 fungal strains. An assay based on electrolyte leakage was used for screening leaves and tuber
tissues with fungal culture filtrates. With almost all cultivars the resistance of leaves did not correlate with the resistance
of tubers. Cv. Ajax appeared the least susceptible in both leaf and tuber tests, while the cv. Prima was the most susceptible
in tuber tests. 相似文献
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"Pure" human hematopoietic progenitors: permissive action of basic fibroblast growth factor 总被引:18,自引:0,他引:18
M Gabbianelli M Sargiacomo E Pelosi U Testa G Isacchi C Peschle 《Science (New York, N.Y.)》1990,249(4976):1561-1564
Methodology has been developed that enables virtually complete purification and abundant recovery of early hematopoietic progenitors from normal human adult peripheral blood. A fraction of the pure progenitors is multipotent (generates mixed colonies) and exhibits self-renewal capacity (gives rise to blast cell colonies). This methodology provides a fundamental tool for basic and clinical studies on hematopoiesis. Optimal in vitro cloning of virtually pure progenitors requires not only the stimulatory effect of interleukin-3, granulocyte-macrophage colony-stimulating factor, and erythropoietin, but also the permissive action of basic fibroblast growth factor. These findings suggest a regulatory role for this growth factor in early hematopoiesis. 相似文献
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A. A. Habashy Testa Mosconi R Caccia A Mazzucato E Santangelo G. P. Soressi 《The Journal of Horticultural Science and Biotechnology》2013,88(2):322-328
SummaryTo breed fruitful triploid tomatoes, diploid (2x) and tetraploid (4x) plants were regenerated from hypocotyl explants, using two pairs of near-isogenic lines differing respectively for the parthenocarpic fruit and parthenocarpic fruit-2 mutations. From 626 regenerated shoots, 53 were tetraploid. In the 4x plants, the mean number of fruits was lower than in the 2x class for all the genotypes, but in the two parthenocarpic lines it was higher than in the respective wild-types at the same ploidy level. Compared with 2x, the mean weight of 4x fruits was lower in wild-type and higher in parthenocarpic lines. Out of four cross combinations between tetraploid and diploid plants (within two parthenocarpic and two corresponding wild-type lines), only two yielded seeds (the parthenocarpic fruit line and its near-isogenic wild-type). Triploids were vegetatively propagated and evaluated in a field trial. Whereas wild-type triploids were completely sterile, the 3x parthenocarpic fruit plants had a fruit set that was not statistically different from that observed in 2x individuals. Mean fruit weight in 3x parthenocarpic plants was also higher than in 2x and comparable with 4x plants. Yield estimates in such triploids were not statistically different from those recorded in diploids, both wild-type and parthenocarpic. Triploid fruits had a soluble solids value higher than 2x fruits. The total seedlessness and the increased dry-matter content appeared traits peculiar to triploid parthenocarpic fruits. Therefore, triploids may deserve interest in breeding fresh market and processing tomatoes, providing that their commercial multiplication is ensured by micropropagation. 相似文献
16.
The reproductive system at the neuroendocrine-immune interface: focus on LHRH,estrogens and growth factors in LHRH neuron-glial interactions 总被引:3,自引:0,他引:3
Morale MC Gallo F Tirolo C L'Episcopo F Gennuso F Testa N Caniglia S Spina-Purrello V Avola R Scoto GM Marchetti B 《Domestic animal endocrinology》2003,25(1):21-46
Bidirectional communication between the neuroendocrine and immune systems plays a pivotal role in health and disease. Signals generated by the hypothalamic-pituitary-gonadal (HPG) axis (i.e. luteinizing hormone-releasing hormone, LHRH, and sex steroids) are major players coordinating the development immune system function. Conversely, products generated by immune system activation exert powerful and longlasting effects on HPG axis activity. In the central nervous system (CNS), one chief neuroendocrine-immune (NEI) compartment is represented by the astroglial cell population and its mediators. Of special interest, the major supporting cells of the brain and the thymus, astrocytes and thymic epithelial cells, share a similar origin and a similar set of peptides, transmitters, hormones and cytokines functioning as paracrine/autocrine regulators. This may explain some fundamental analogies in LHRH regulation of both cell types during ontogeny and in adult life. Hence, the neuropeptide LHRH significantly modulates astrocyte and thymic cell development and function. Here we focus this work on LHRH neuron-glial signaling cascades which dictate major changes during LHRH neuronal differentiation and growth as well as in response to hormonal manipulations and pro-inflammatory challenges. The interplay between LHRH, growth factors, estrogens and pro-inflammatory mediators will be discussed, and the potential physiopathological implications of these findings summarized. The overall study highlights the plasticity of this intersystem cross-talk and emphasize neuron-glial interactions as a key regulatory level of neuroendocrine axes activity. 相似文献
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Luigi Faino Paola Carli Antonino Testa Gennaro Cristinzio Luigi Frusciante Maria Raffaella Ercolano 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(2):233-241
Tomato is challenged by several pathogens which cause loss of production. One such pathogen is the oomycete Phytophthora infestans which is able to attack all the aerial parts of the plant. Although a wide range of resistance sources are available, genetic
control of this disease is not yet successful. Pyramiding R-genes through genetic transformation could be a straightforward
way to produce tomato and potato lines carrying durable resistance to P. infestans. In this work the R1 potato gene was transferred into tomato lines. The tomato transgenic lines were analyzed by using q-RT-PCR
and progeny segregation to determine the gene copy number. To test the hypothesis that R1 represents a specifically regulated R-gene, transgenic tomato plants were inoculated with P. infestans isolate 88133 and IPO. All the plants containing the R1 gene were resistant to the late blight isolate IPO-0 and susceptible to isolate 88133. These results provide evidence for
specific activation of the R1 gene during pathogen challenge. Furthermore, evidence for enhancement of PR-1 gene expression during P. infestans resistance response was obtained. 相似文献
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Lippman A Newman SA Testa G Harris J 《Science (New York, N.Y.)》2005,307(5709):515-7; author reply 515-7