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21.
Fernández F Pinacho DG Sánchez-Baeza F Marco MP 《Journal of agricultural and food chemistry》2011,59(9):5036-5043
An inexpensive and portable surface plasmon resonance (SPR) sensor, SPReeta Evaluation Kit SPR3, has been used to develop a biosensor for the determination of fluoroquinolone antibiotics (FQs) and to demonstrate its performance analyzing FQ residues in milk samples. The SPReeta three-channel gold chips were activated with a mixed self-assembled monolayer (m-SAM) and functionalized with a FQ haptenized protein. Binding of the antibody produced a concentration-dependent increase of the SPR signal as a result of the change in the refraction index. Similarly, the presence of the FQ produced a dose-dependent decrease of the response, which allowed a good limit of detection (LOD) to be obtained (1.0 ± 0.4 μg L(-1) for enrofloxacin in buffer). The response was reproducible in all three channels, on different injections and days, and also between chips. Milk samples could be analyzed after a simple sample treatment involving fat removal by centrifugation and dilution with water. Under these conditions calibration curves were obtained showing that FQ residues can be analyzed in milk samples with an IC(50) value of 26.4 ± 7.2 μg L(-1) and a LOD of 2.0 ± 0.2 μg L(-1) (for enrofloxacin), far below the European Union regulations for this antibiotic family in this matrix. Finally, the paper also demonstrates that the biosensor is able to selectively detect the presence of FQs in milk samples, even in the presence of other antibiotics. Enrofloxacin, ciprofloxacin, and norfloxacin residues were detected in blind samples supplied by Nestle? Co. 相似文献
22.
Chiara De Lucchi Piergiorgio Stevanato Linda Hanson Mitch McGrath Lee Panella Marco De Biaggi Chiara Broccanello Marco Bertaggia Luca Sella Giuseppe Concheri 《Euphytica》2017,213(3):71
Fusarium spp. cause severe damage in many agricultural crops, including sugar beet, with Fusarium oxysporum historically being considered as the most damaging of all species. Sugar beet needs to be protected from this class of soil-borne pathogens in order to ensure an optimal sugar yield in the field. Genetic control of the disease is crucial in managing these pathogens. Identification of single nucleotide polymorphism (SNP) markers linked to resistance can be a powerful tool for the introgression of valuable genes needed to develop Fusarium-resistant varieties. A candidate gene approach was carried out to identify SNP markers linked to putative Fusarium resistance sources in sugar beet. Five resistant analogue genes (RGAs) were screened by means of high resolution melting (HRM) analysis in a set of sugar beet lines, considered as resistant and susceptible to Fusarium oxysporum. HRM polymorphisms were observed in 80% of amplicons. Two HRM polymorphisms were significantly associated with Fusarium resistance (P < 0.05). The amplicons that showed association were sequenced and two SNPs were identified. The association was further validated on 96 susceptible and 96 resistant plants using competitive allele-specific PCR (KASPar) technology. The selected SNPs could be used for marker-assisted breeding of Fusarium resistance in sugar beet. 相似文献
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Venturi L Rocculi P Cavani C Placucci G Dalla Rosa M Cremonini MA 《Journal of agricultural and food chemistry》2007,55(26):10572-10578
Hydration of freeze-dried chicken breast meat was followed in the water activity range of aw=0.12-0.99 by a multianalytical approach comprising of sorption isotherm, differential scanning calorimetry (DSC), and nuclear magnetic resonance (NMR). The amount of frozen water and the shape of the T2-relaxogram were evaluated at each water content by DSC and NMR, respectively. Data revealed an agreement between sorption isotherm and DSC experiments about the onset of bulk water (aw=0.83-0.86), and NMR detected mobile water starting at aw=0.75. The origin of the short-transverse relaxation time part of the meat NMR signal was also reinvestigated through deuteration experiments and proposed to arise from protons belonging to plasticized matrix structures. It is proved both by D2O experiments and by gravimetry that the extra protons not contributing to the water content in the NMR experiments are about 6.4% of the total proton NMR CPMG signal of meat. 相似文献
25.
Kyriakos C. Prousis Stefanos Kikionis Efstathia Ioannou Silvia Morgana Marco Faimali Veronica Piazza Theodora Calogeropoulou Vassilios Roussis 《Marine drugs》2022,20(1)
Marine biofouling is an epibiotic biological process that affects almost any kind of submerged surface, causing globally significant economic problems mainly for the shipping industry and aquaculture companies, and its prevention so far has been associated with adverse environmental effects for non-target organisms. Previously, we have identified bromosphaerol (1), a brominated diterpene isolated from the red alga Sphaerococcus coronopifolius, as a promising agent with significant antifouling activity, exerting strong anti-settlement activity against larvae of Amphibalanus (Balanus) amphitrite and very low toxicity. The significant antifouling activity and low toxicity of bromosphaerol (1) motivated us to explore its chemistry, aiming to optimize its antifouling potential through the preparation of a number of analogs. Following different synthetic routes, we successfully synthesized 15 structural analogs (2–16) of bromosphaerol (1), decorated with different functional groups. The anti-settlement activity (EC50) and the degree of toxicity (LC50) of the bromosphaerol derivatives were evaluated using cyprids and nauplii of the cirriped crustacean A. amphitrite as a model organism. Derivatives 2, 4, and 6–16 showed diverse levels of antifouling activity. Among them, compounds 9 and 13 can be considered as well-performing antifoulants, exerting their activity through a non-toxic mechanism. 相似文献
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27.
Patrizia Spigaglia Fabrizio Barbanti Fabio Marocchi Marco Mastroleo Marco Baretta Patrizia Ferrante Emilia Caboni Simona Lucioli Marco Scortichini 《Plant pathology》2020,69(4):765-774
Since 2012, a new pathogenic syndrome has frequently been observed in many areas of kiwifruit cultivation in Italy. The main symptoms include an initial withering of the leaves followed by a total and sudden collapse of plants, mainly occurring during summer. The withered leaves fall and the main and secondary feeder roots appear rotten, sometimes showing a reddish-brown discoloration. The disease, that affects both the green and yellow-fleshed cultivars, has been called kiwifruit vine decline and is locally known as moria. The syndrome has been found consistently associated with soil waterlogging, which frequently occurs either after the traditional agronomical practice of irrigating orchards through surface irrigation or after very heavy rainfall. So far, the role played by bacteria in this syndrome has not been investigated. In the present study, Clostridium spp. were isolated from both rotten roots and soils obtained from Italian kiwifruit orchards affected by the syndrome, indicating for the first time that anaerobic bacteria are able to cause damage to woody crops. C. bifermentans and C. subterminale incited symptoms in kiwifruit in both in vivo and in vitro pathogenicity tests. Soil waterlogging seems to potentially favour colonization of kiwifruit roots by anaerobic bacteria, probably because saturation of the soil can facilitate proliferation and persistence of these bacteria during long periods of the vegetative growth of the crop. The occurrence of anaerobic bacteria does not exclude the possibility that other microorganisms can play additional/synergic role(s) in causing the kiwifruit vine decline. 相似文献
28.
The effects of starving and feeding on Dover sole (Solea solea,Soleidae, Linnaeus, 1758) stress response and early larval development
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Chiara Carla Piccinetti Marco Donati Giuseppe Radaelli Giovanni Caporale Gilberto Mosconi Francesco Palermo Lina Cossignani Roberto Salvatori Rocio Penuela Lopez Ike Olivotto 《Aquaculture Research》2015,46(10):2512-2526
In the view of an urgent necessity to improve the quantity and the quality of farmed fish species, there is a strong need to improve our basic knowledge on the effects of first feeding during the developmental stages of fish larvae. High mortality, mainly due to food deprivation or inappropriate food quality, has been observed in many larval fish species, but knowledge about the morphological, biochemical and molecular processes related to this topic is still poorly understood. The understanding of the early larval ontogeny as well as the larval nutritional requirements and the molecular and cellular mechanisms elicited by fish larvae during food deprivation and starvation are thus of primary importance. At this regard, this study investigates, in Dover sole larvae, the effects of starvation and starving/re‐feeding procedures at a morphological, histological, biochemical and molecular level. The results evidenced that starved larvae progressively decrease in growth, lipid content, affected their gastrointestinal tract and muscle development and increased cortisol and heat shock protein 70 levels. On the contrary, starved and re‐fed larvae showed, after the restoration of a favourable feeding condition, a compensatory growth. In conclusion, this is the first study analysing through a multidisciplinary approach the effects of food deprivation on the development of an important economic species, the Dover sole. 相似文献
29.
- 1. Loop models of ecological and socio‐economic systems were developed to analyse and predict the impact of a possible accidental introduction (escapes) of the abalone Haliotis discus hannai into a benthic community of north‐central Chile.
- 2. Although the ‘new’ ecological system resulting from a successful invasion of abalone would be locally stable, the establishment of a self‐enhanced dynamic of recruits would transform this into an unstable system.
- 3. The harvest of the kelp, Lessonia trabeculata and other macroalgae is not recommended because this destabilizes the system. The harvest of abalone adults is only sustainable if they do not exert a negative effect upon other native invertebrates.
- 4. The eco‐social model showed three important results: (1) if the variables ‘Price’ and ‘Farming’ are in expansion and stationary dynamics, then the models were found unstable; (2) a self‐enhanced dynamic of abalone recruits tends toward instability; and (3) the harvest of the kelp L. trabeculata and other macroalgae would be non‐sustainable.
- 5. Based on our results, the sustainable development of extensive farming of H. discus hannai in the sea would be not reached. If it is done, an intensive monitoring of the community after introduction into the system is strongly recommended. Likewise, the farming of macroalgal species (source of food for abalone) should be promoted in order to avoid harvesting of macroalgae from natural systems.
30.
Bravo Carlo Millo Christian Covelli Stefano Contin Marco De Nobili Maria 《Journal of Soils and Sediments》2020,20(2):1074-1086
Journal of Soils and Sediments - Humic acids (HA) have several environmental roles, but are particularly important in aquatic environments, being recognized as redox active natural organic matter... 相似文献