油茶炭疽病的发生与蔓延

四年来,作者对油茶炭疽病的侵染途径、传播方式、发病的阶段性、特点和具体条件职及病害蔓延等規律进行了研究。 結果指出,病菌侵染途径以伤口为主,也可能从自然孔道侵害果实。潛育期随气温上升而縮短,15℃至28℃时,潛育期由15天缩短为5天。分生孢子借助雨滴、风夹雨进行传播,干燥气流不能传菌。林間象(虫甲)、螞蚁不是传病的主要媒介。病害发生具有鮮明的季节性。果实发病期长,自4月至10月。病害有初期、中期、盛期和末期四个时期。感病宿主存在、菌源量积累和高温降雨为病害发生、发展的决定性因素。病害发展有間歇期和消长期。植株发病类型有:早发型、中发型、后发型及突发型四种。病果类型有:内侵型、邻侵型和外侵型三种。油茶炭疽病的逐年蔓延速度在一年内为36.5—50.0％;二年内为51.9—60.3％;三年内为55.8—61.8％。     

Biochemical polymorphisms in Spanish Avileña-Negra Iberica cattle

SUMMARY: Thirteen biochemical blood polymorphisms were analysed in a population of 149 Spanish Avile?a-Negra Ibérica cattle. The study revealed variation at the following nine loci: HBB, CA, NP, CP, AMY1, ALB, GC, TF and PTF2. The following systems were monomorphic: CAT, DIA1, MDH1 and ME1. Using polyacrylamide-gel electrophoresis, a slow, migrating pair of bands was found in the GC protein system. This pattern is probably controlled by the GC(C) allele, described in only a few cases in cattle. Furthermore, starch-gel electrophoresis allowed the detection of a variant with intermediate mobility between the ALB(A) and the ALB(B) alleles at the albumin locus. A variant with a similar electrophoretic pattern has occasionally been reported in cattle. However, utilizing IEF under denaturing conditions, such a variant could not be differentiated from the ALB(A) allele and thus its significance is not clear. ZUSAMMENFASSUNG: Biochemischer Polymorphismus in spanischen Avile?a-Negra Iberica Rindern Insgesamt 13 biochemische Systeme wurden in einer Population von 149 spanischen Avilena-Negra-Iberika-Rindern hinsichtlich Polymorphismus analysiert. Es zeigten sich Varianten bei folgenden Loci: HBB, CA, NP, CP, AMY1, ANB, GC, TF und BTF2, w?hrend CAT, DEA, MDH1 und ME1 monomorph sind. Bei st?rke Gel-Elektrophorese wurde im Albumin-Locus eine Variante mit intermedi?rer Mobilit?t zwischen ALB(A) und ?LB(B) Allel entdeckt. Eine solche Variante wurde bisher nur sehr selten bei Rindern beobachtet. Darüber hinaus wurde bei Polyacrylamid-Gel-Elektrophorese ein langsam wanderndes Paar von B?ndern im GC-Proteinsystem gefunden. Dieses Muster ist wahrscheinlich von dem selten vorkommenden GC(C) -Allel verursacht. RESUMEN: Se analizaron trece polimorfismos bioquímicos sanguíneos en una población de 149 animales de la raza Avile?a-Negra Ibérica de ganado vacuno. El estudio reveló la existencia de variación en los nueve loci siguientes: HBB, CA, NP, CP, AMY1, ALB, GC, TF y PTF2. Fueron monomórficos los sistemas siguientes: CAT, DIA1, MDH1 y ME1. Utilizando eletroforesis en gel de poliacrilamida se encontró un par de bandas de migración lenta en el sistema de la proteína GC. Este patrón probablemente está controlado por el infrecuente alelo GC(C) , descrito en unos pocos casos en el ganado vacuno. Además, la electroforesis en gel de almidón permitió detectar en el locus de la albúmina una banda con movilidad intermedia entre los alelos ALB(A) y ALB(B) . Una variante con un patrón electroforético similar ha sido descrita en muy pocas ocasiones en el ganado vacuno. Sin embargo, la utilización de IEF en condiciones desnaturalizantes no permitió diferenciar esta variante del alelo ALB(A) y, por lo tanto, el significado de la misma no está claro.     

Correct equations for comparing models in diallel analysis

SUMMARY: Correct equations are given to express the parameter estimates of four models for complete diallels as a function of the parameter estimates in the model of Eisen et al. (1983). In recent literature these equations have been partly incorrect. ZUSAMMENFASSUNG: Korrekte Gleichungen für den Vergleich von Modellen in der Diallelanalyse Für die Darstellung der Parametersch?tzwerte von vier Modellen für vollst?ndige Diallele als Funktion der Parametersch?tzwerte des Modells von Eisen et al. (1983), die in der Literatur teilweise fehlerhaft erfolgte, werden korrekte Formeln angegeben.     

Enzyme arrangement of various tissues in swine. 1. Studies of the effect of 32 hours of fasting, administration of actinomycin D, and fasting in combination with administration of actinomycin D on crude protein level, activities of GOT, GTP, fructose-1,6-diphoaphatase as well as ATPase of liver, kidneys and semitendinous muscle in newborn piglets]

The effects of sufficient milk intake as well as of 32 hours of fasting after birth, administration of actinomycin D (intraperitoneal application of 1 mg/kg five weight), and fasting in combination with actinomycin D on the development of body and liver weights, crude protein levels in homogenate and supernatant of liver, kidneys, and M. semitendinosus as well as on the activities of certain tissue enzymes were analysed with four groups of piglets (n = 4). Fasting, administration of actinomycin, and fasting in combination with actinomycin D resulted in rapid reduction in body and liver weights, while the crude protein levels in those tissues were not affected with significance. GOT and fructose-1,6-diphosphatase activities in supernatant from liver tended to decline under fasting conditions. The ATPase activity in the homogenate of the above tissues did not change in response to differentiated treatment.     

The subacute and subchronic toxicity of chlorophacinone in Japanese quail (Coturnix c. japonica)

Accordingly to the subacute toxicity of chlorphacinone to Japanese quail (LC 50: 60 mg/kg feed), the uptake of Delicia-chlorphacinone-baits for several days can be lethal to birds as a matter of principle. A subchronic study resulted in a "no-observed-effect-level" of 1 mg chlorphacinon/kg feed. Reproduction is not affected until dose levels which resulted in lethality or are accompanied by physiological changes (coagulability of blood, microcytic anaemia). Increased sensibility of descendants to chlorphacinone cannot be derived from the findings.     

First record of bacillary rickettsia-like organisms in European tick Dermacentor marginatus (Sulzer).

Bacillary rickettsia-like organisms (BRLO) were found in the tick Dermacentor marginatus. They are gram-negative and differ from common bacteria and reckettsiae both in the cultivation conditions and morphology. BRLO are non-pathogenic for ticks and guinea pigs. In our studies they were isolated on half-engorged females of D. marginatus, on which they are still maintained.     

Excessive oral administration of iron to sheep. 1. Digestibility of rations, results of fattening and carcass yields]

4 digestibility trials (4 male sheep per group) and 2 growth trials were carried out with 24 (V 1) and 32 (V 2) fattening lambs to investigate the effect of abundant oral Fe doses on the digestibility of crude nutrients in a ration of barley + dried green feed and on the results of fattening and carcass yields. Fe supplementation (less than 1400 mg per sheep/day) did not significantly change the digestibility coefficients of crude nutrients. Increasing Fe levels in the concentrates decreased the rates of liveweight increase and food consumption in both growth trials. Well-established statistical evidence was provided for the decline in absolute carcass yields resulting from the lower weight of the lambs at the end of the fattening period after Fe supplementation.     

Histological studies on cow intestinal mucosa and electron microscopic studies of the ruminal epithelium of cows fed a deficient diet for a prolonged time]

Histological and histochemical studies were carried out on the gastro-intestinal mucosa of three experimental cows (roughages/maize silage) and three control animals. The animals were slaughtered on termination of the long-term trial. Mucosa samples were taken, for further study, from the rumen, the duodenum, the jejunum, the large intestine and the appendix. Histochemical analysis did not reveal any essential differences in the activities of non-specific esterase, alkaline and acid phosphatase and lactic acid dehydrogenase in the mucosa of the rumen, the large and the small intestine and the appendix of both the experimental animals and the controls. The experimental animals were found to exhibit a higher rate of glutamate-dehydrogenase activity in the ruminal mucosa and in the mucosa of the large and small intestine. A higher succinate dehydrogenase activity was observed in the ruminal mucosa of the experimental animals, relative to that of the controls, while the activity in the intestinal mucosa was decreased. Only slight changes were noted in the activity of the enzymatic systems tested. Electron microscopic studies did not reveal any differences in the ultrastructure of the epithelial cells of the ruminal mucosa in both the experimental animal and the controls.     

Testing of the protective effect of cell-free lyophilized vaccine against Marek's disease in field trials]

The protective effect of a lyophilized vaccine against Marek's disease ("Keramvac"--Pfizer), prepared from a cell-free turkey's herpesvirus (strain FC 126), was compared in field trials with losses in the group of non-vaccinated chickens. Under the indicated conditions, the vaccine had only a 50.86% effectiveness. The possible causes of the reduced vaccination effect are discussed with regard to the pathomorphological and virological findings suggesting, among others, an increased incidence of the symptoms of the classical form of Marek's disease in the population investigated.