有机质肥料中硝酸態氮素分析方法的研究

一、绪言硝酸态氮素定量分析,普通多使用还原法与比色法两种。但有机质肥料如厩肥、堆肥含大量有色体、尿素、氯化物等杂质,以上两种方法,都不适用。动物粪便中,每含有大量铵盐及易于分解的各程氮的化合物。无论在酸性或     

1-methylcyclopropene increases storability and shelf life in climacteric and nonclimacteric plums

The effect of 1-methylcyclopropene (1-MCP) at three different doses (0.25, 0.50, and 0.75 microL L(-1)) on the ripening processes of a climacteric, cv. Santa Rosa, and a suppressed climacteric type, cv. Golden Japan, plum was studied. For both cultivars, positive effects were observed in terms of inhibition of ethylene production and delays of the physical, chemical, and biochemical changes associated with ripening. 1-MCP-treated plums were firmer with lower weight loss, reduced degrees Brix/titratable acidity ratios, and lower color changes during cold storage and subsequent shelf life at 20 degrees C than controls. For most factors, the effectiveness of 1-MCP was dose-dependent in Santa Rosa but dose-independent for Golden Japan.     

Influence of harvest date and crop yield on the fatty acid composition of virgin olive oils from cv. Picual

In this study was analyzed the effect of crop year and harvesting time on the fatty acid composition of cv. Picual virgin olive oil. The study was carried out during the fruit ripening period for three crop seasons. The mean fatty acid composition of Picual oils was determined. The oils contained palmitic acid (11.9%), oleic acid (79.3%), and linoleic acid (2.95%). The content of palmitic acid and saturated fatty acids decreased during fruit ripening while oleic and linoleic acids increased. The amount of stearic and linolenic acids decreased. The amount of saturated acids, palmitic and stearic, and the polyunsaturated acids linoleic and linolenic was dependent on the time of harvest, whereas the amount of oleic acid varied with the crop year. The differences observed between crop years for both palmitic and linoleic acid may be explained by the differences in the temperature during oil biosynthesis and by the amount of summer rainfall for oleic acid content. A significant relationship was observed between the MUFA/PUFA ratio and the oxidative stability measured by the Rancimat method.     

Analysis of virgin olive oil volatiles by a novel electronic nose based on a miniaturized SAW sensor array coupled with SPME enhanced headspace enrichment

A novel electronic nose based on solid-phase microextraction (SPME) coupled with a surface acoustic wave (SAW) sensor array has been used to analyze different quality virgin olive oils. A mathematical model was designed with 37 samples to distinguish lampante from the other virgin olive oils categories (extra-virgin and virgin), because lampante-virgin olive oils cannot be consumed without a previous refining process. The model, successfully validated with a test set of 16 samples, was able to classify 90% of the samples correctly. Misclassifications were explained by SPME-HRGC analyses and a second sensory evaluation.     

Subcellular localization and isoenzyme pattern of peroxidase and polyphenol oxidase in beet root (Beta vulgaris L.)

The two enzymes involved in enzymatic browning reactions, polyphenol oxidase (PPO) and peroxidase (PO), have been partially purified and extracted from different fractions of beet root. PPO is mainly located in the membrane fraction, and it was also found in the soluble fraction. In both cases PPO was in its latent state. However, PO activity was higher in the soluble fraction than in the membrane fraction. Nevertheless, the highest values of specific activity for PO were obtained from the solubilized enzyme from acetone powders. Under native isoelectric focusing (IEF), several PPO isoenzymes were present in the pH range of 4.8-5.8. All of these isoenzymes shared a single band with a similar apparent mass under sodium dodecyl sulfate-polyacrylamide gel electrophoresis. PO was also analyzed by IEF, showing a complex isoenzyme pattern in all fractions. The characteristic basic PO isoenzyme of high pI found in both the soluble fraction and the solubilized enzyme from acetone powders was not detected in the membrane fraction. The kinetic characterization of PPO and PO from all fractions was carried out.     

From on-farm solid-set sprinkler irrigation design to collective irrigation network design in windy areas

In this paper, a contribution to the design of collective pressurised irrigation networks in solid-set sprinkler-irrigated windy areas is presented. The methodology is based on guaranteeing minimum on-farm performance, using a historical hourly wind speed database and a ballistic solid-set irrigation simulation model. The proposed method was applied to the Montesnegros Irrigation District (central Ebro basin, Spain). The district irrigates an area of 3493 ha using an on-demand schedule. The average wind speed in the area is 2.8 m s⁻¹. An analysis of district water records showed that farmers often reduce water demand when the wind speed is high, but their irrigation decision making is limited by the capacity of the irrigation network and by the unpredictable character of local winds. Simulations were performed for 11 irrigation seasons, 2 triangular sprinkler spacings (18 m × 18 m and 18 m × 15 m), and 2 sprinkler models. The percentage of monthly suitable time for irrigation was determined for four management strategies. The first one was based on a wind speed threshold (3 m s⁻¹), while the other three were based on three levels (standard, relaxed and restrictive) of two irrigation performance parameters: the Christiansen Uniformity Coefficient (CU) and the Wind Drift and Evaporation Losses (WDEL). The standard strategy classified the time as suitable for irrigation when CU ≥ 84% and WDEL ≤ 20%. The thresholds limits of the irrigation parameters for the relaxed strategy were CU ≥ 80% and WDEL ≤ 25%. Finally, the restrictive strategy used thresholds of CU ≥ 90% and WDEL ≤ 15%. The suitable time for the first strategy (56%) was always lower than for the standard and the relaxed strategies (with respective average values of 75 and 86%), and higher than for the restrictive strategy (30%). In order to design the collective network, the hydrant operating time was equalled to the suitable time for irrigation. The differences in the cost of the collective network plus the on-farm equipment were particularly relevant between the restrictive strategy and the other three. Differences in suitable operating time were clear between sprinkler spacings, and less evident between sprinkler models. The application of the proposed methodology may be limited by the availability of historical wind speed records and CU estimates for different combinations of sprinkler models, sprinkler spacings and wind speed.     

Effects of tetracycline administration on the proteomic profile of pig muscle samples (L. dorsi)

Effect of tetracycline (TC) administration on the proteomic profile of pig muscle was evaluated by 2D electrophoresis and MALDI-TOF mass spectrometry. The TC content at slaughter was determined in L. dorsi samples by HPLC-DAD. Mean residual concentration of TC in the muscle of treated animals, calculated as the sum of TC and epi-TC was 126.3 microg/kg, indicating a rapid elimination of TC in this tissue. Several differential spots (n = 54, p < 0.05) were observed in protein profiles from control and treated animals. MALDI-TOF identification gave a positive match for 5 differential spots, that is, glycerol-3-phosphate dehydrogenase 1 (G3PD1), phosphoglycerate kinase 1, novelprotein (0610037L13Rik), leucine aminopeptidase 3 (LAP), and hypothetical protein isoform 2. Results show that proteomics could be a useful tool to reveal pharmacological treatments with TC, even if the possible uses of differential spots as biomarkers to detect illegal administration of TC require further studies. Different spot patterns as a consequence of TC treatments seem to be another interesting issue for the consequences on tissue metabolism and meat quality.     

The Ischigualasto Tetrapod Assemblage (Late Triassic, Argentina) and 40Ar/39Ar Dating of Dinosaur Origins

(40)Ar/(39)Ar dating of sanidine from a bentonite interbedded in the Ischigualasto Formation of northwestern Argentina yielded a plateau age of 227.8 +/- 0.3 million years ago. This middle Carnian age is a direct calibration of the Ischigualasto tetrapod assemblage, which includes some of the best known early dinosaurs. This age shifts last appearances of Ischigualasto taxa back into the middle Carnian, diminishing the magnitude of the proposed late Carnian tetrapod extinction event. By 228 million years ago, the major dinosaurian lineages were established, and theropods were already important constituents of the carnivorous tetrapod guild in the Ischigualasto-Villa Unión Basin. Dinosaurs as a whole remained minor components of tetrapod faunas for at least another 10 million years.     

Plant growth-rate dependence of detrital carbon storage in ecosystems

Detrital carbon accumulation accounts for most of an ecosystem's capacity to store organic carbon because the carbon contained as plant detritus exceeds that stored in living plants by about threefold. A comparative analysis of the mass and turnover of detrital carbon in ecosystems demonstrates that these properties are strongly related to the turnover rate of the dominant primary producers and are poorly related to ecosystem primary production. These results contribute to an understanding of the factors that control carbon storage in ecosystems and the role of carbon storage in the global carbon budget.