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61.
Arbutus unedo L. (strawberry tree, Ericaceae) is a woody species with a circum-Mediterranean distribu-tion. It has considerable ecological relevance in southern... 相似文献
62.
M.F. Madeira F.B. Figueiredo A.G.S. Pinto L.D. Nascimento M. Furtado E. Mouta-Confort C.C. de Paula A. Bogio M.C.A. Gomes A.M.S. Bessa S.R.L. Passos 《Research in veterinary science》2009,87(2):260-262
The objective of this study was to evaluate intact skin of seroreactive dogs as a possible target for the parasitological confirmation of canine visceral leishmaniasis (CVL). For this purpose, 394 dogs identified in serological surveys carried out in the metropolitan region of Belo Horizonte were studied. Blood was collected from all animals for serology and a tissue sample was obtained from two sites for parasitological diagnosis. Skin obtained from the ear and scapular region was simultaneously analyzed in 247 animals and lesion samples and ear skin were analyzed in 147 dogs. Leishmania parasites were isolated from 310 (78.7%) animals, and all isolates were identified as Leishmania chagasi. Simultaneous isolation from two sites was possible in 240 of the 310 animals, including ear and scapular skin in 151/247 (61.1%) and ear skin and skin lesions in 89/147 (60.5%). Ours results suggest that intact skin is one of the main target sites for the parasitological confirmation of CVL in seroreactive dogs. 相似文献
63.
64.
Samuel Luiz Fioreze João Paulo Costa Carneiro Daniela Dias Pinto João Domingos Rodrigues 《Journal of plant nutrition》2017,40(18):2602-2610
We characterized the pollination and fecundation times in soybean flowers and evaluated the effects of kinetin and calcium applications on physiological and productive traits of soybean plants during the reproductive stage. The anatomical study of flowers of eight soybean cultivars showed that fecundation occurred in closed flowers with visible petals, which presented embryo in the first cell divisions. These results indicate that fertilizers and growth regulation applications should be performed before flower opening, which is different from the current recommendation. Foliar applications of kinetin and calcium between the floral bud and full flowering stages did not affect carbon dioxide (CO2) assimilation, yield components and final yield. The results obtained in this research showed the lack of viability of foliar application of calcium and kinetin in order to increase pod set and number of seeds per plant in soybean crop. 相似文献
65.
Larissa Sarmento dos Santos Joicy Cortez Sá Diego Luiz dos Santos Ribeiro Nancyleni Pinto Chaves Juliana Pinto da Silva Mol Renato Lima Santos Tatiane Alves da Paixão Alcina Vieira de Carvalho Neta 《Tropical animal health and production》2017,49(4):675-679
The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State. 相似文献
66.
T. F. Raso A. O. T. Carrasco J. C. R. Silva M. F. V. Marvulo A. A. Pinto 《Zoonoses and public health》2010,57(6):411-416
To evaluate the prevalence of antibodies to Chlamydophila psittaci 364 serum samples were collected from veterinarians, biologists, animal scientists, veterinary students, animal keepers and others employees in 20 zoos, and from veterinary practitioners in 10 Brazilian states. Subjects ranged from 15 to 64 years of age, with 268 (74%) males and 96 (26%) females. Chlamydial antibodies were determined by the complement fixation test (CFT) and specific anti‐C. psittaci IgG antibodies were determined by the microimmunoflurescence (MIF) test. Complement fixation test showed 23.9% (87/364) and MIF test showed 4.7% (17/364) positive serum samples. Titres ranged from 16 to 256 in both assays, demonstrating evidence of recent or current infection. Although chlamydial antibodies were detected in workers of seventeen zoos, MIF test only detected specific C. psittaci antibodies in seven of them. Previous psittacosis infection was suspected in eight workers of two zoos, five of whom reported having pneumonia, while employed at the zoos. However, diagnosis was not established in any of these cases in the past. Results indicated the occurrence of infection and previous contact of Brazilian zoo workers with C. psittaci, as well as the zoonotic potential of psittacosis in this risk population. Other studies are necessary to evaluate the risk factors of infection in this population. This seroepidemiological survey confirmed the need to adopt preventive measures to control avian chlamydiosis and protect the health of zoo workers in the country. 相似文献
67.
Carlos Alberto Scapim Cleso Antônio Patto Pacheco Antônio Teixeira do Amaral Júnior Rafael Augusto Vieira Ronald José Barth Pinto Thiago Vincenzi Conrado 《Euphytica》2010,174(2):209-218
In plant breeding, correlations between the statistics of stability and adaptability of popcorn cultivars are not yet well
understood. Therefore, the objectives of the present experiment was to investigate the correlations between sdi2 \sigma_{\rm di}^{2} and bi \beta_{\rm i} from Eberhart and Russell, ωi from Wricke,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} and
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} from Huehn, Pi from Lin and Binns and the rank-sum from Kang, and indicate the most reliable method for selecting popcorn cultivars. These
statistics were estimated by data of crop yield from 19 Brazilian genotypes under 21 environments and popping expansion under
16 environments. The ωi,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} ,
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} and sdi2 \sigma_{\rm di}^{2} were positively and significantly correlated indicating that just one in these five statistics is sufficient for selecting
stable genotypes although they were not correlated with the means of crop yield and popping expansion. The bi \beta_{\rm i} was negatively and significantly correlated with Pi for crop yield indicating that the most adaptable genotypes tend to have the lowest estimates of Pi. Although Pi was not correlated with ωi,
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} ,
\textS\texti(2) {\text{S}}_{\text{i}}^{(2)} ,
\textS\texti(3) {\text{S}}_{\text{i}}^{(3)} , or sdi2 \sigma_{\rm di}^{2} statistics, it displayed positive correlation with the Index 1 (crop yield and popping expansion +
\textS\texti(1) {\text{S}}_{\text{i}}^{(1)} rank) and Index 2 (crop yield and popping expansion + Wi) indicating that superior popcorn genotypes are also stable. Finally, both Pi and the rank-sum are useful statistics in breeding programmes where crop yield, popping expansion and stability are essential
traits for selecting genotypes. 相似文献
68.
69.
Aldair JW Pinto Maria M Figueiredo Fabiana L Silva Trycia Martins Marilene SM Michalick Washington L Tafuri Wagner L Tafuri 《Acta veterinaria Scandinavica》2011,53(1):67
Background
The aim of this study was to provide a systematic pathological and parasitological overview of the gastrointestinal tract (GIT), including the stomach, duodenum, jejunum, ileum, caecum and colon, of dogs naturally infected with Leishmania.Methods
Twenty mongrel dogs naturally infected with Leishmania (Leishmania) infantum and obtained from the Control Zoonosis Center of the Municipality of Ribeirão das Neves, Belo Horizonte Metropolitan area, Minas Gerais (MG) state, Brazil, were analyzed. The dogs were divided into two groups: Group 1 comprised nine clinically normal dogs and group 2 comprised 11 clinically affected dogs. After necropsy, one sample was collected from each GIT segment, namely the stomach, duodenum, jejunum, ileum, caecum and colon. Furthermore, paraffin-embedded samples were used for histological and parasitological (immunohistochemistry) evaluation and a morphometrical study were carried out to determine the parasite load (immunolabeled amastigote forms of Leishmania). The Friedman and the Mann Whitney tests were used for statistical analysis. The Friedman test was used to analyze each segment of the GIT within each group of dogs and the Mann Whitney test was used to compare the GIT segments between clinically unaffected and affected dogs.Results
The infected dogs had an increased number of macrophages, plasma cells and lymphocytes, but lesions were generally mild. Parasite distribution in the GIT was evident in all intestinal segments and layers of the intestinal wall (mucosal, muscular and submucosal) irrespective of the clinical status of the dogs. However, the parasite load was statistically higher in the caecum and colon than in other segments of the GIT.Conclusion
The high parasite burden evident throughout the GIT mucosa with only mild pathological alterations led us to consider whether Leishmania gains an advantage from the intestinal immunoregulatory response (immunological tolerance). 相似文献70.
Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle 总被引:1,自引:0,他引:1
Borsuk S Andreotti R Leite FP Pinto Lda S Simionatto S Hartleben CP Goetze M Oshiro LM Matos Mde F Berne ME 《Veterinary parasitology》2011,177(1-2):33-38
Neosporosis is of alarming economic concern in the cattle industry. The effectiveness of diagnostic tests for detecting specific antibodies against Neospora caninum is hampered by potential cross-reaction with other coccidia. Use of a single specific antigen might improve test specificity. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using the truncated protein NcSRS2 expressed in Escherichia coli. The ELISA results were compared with those of the indirect fluorescence antibody test (IFAT). Receiver Operating Characteristic (ROC) and Tests in the Absence of a Gold Standard (TAGS) analysis revealed an assay having 96% specificity and 95% sensitivity when applied to 145 positive and 352 negative sera from two distinct cattle populations. Using OD ≤ 0.095 as the cut-off point, the assay's negative and positive predictive values ranged from 98.8% to 50.8% and from 58.8% to 99.1%, respectively, depending on neosporosis prevalence in a given area. The novel ELISA-NcSRS2 format described in the present report constitutes a specific and sensitive method for detecting N. caninum in cattle. 相似文献