全文获取类型
收费全文 | 132647篇 |
免费 | 7272篇 |
国内免费 | 93篇 |
专业分类
林业 | 5389篇 |
农学 | 4272篇 |
基础科学 | 832篇 |
17068篇 | |
综合类 | 21493篇 |
农作物 | 5400篇 |
水产渔业 | 6695篇 |
畜牧兽医 | 68056篇 |
园艺 | 1807篇 |
植物保护 | 9000篇 |
出版年
2020年 | 1183篇 |
2019年 | 1381篇 |
2018年 | 2035篇 |
2017年 | 2332篇 |
2016年 | 2097篇 |
2015年 | 1746篇 |
2014年 | 2231篇 |
2013年 | 5293篇 |
2012年 | 4123篇 |
2011年 | 5113篇 |
2010年 | 3257篇 |
2009年 | 3394篇 |
2008年 | 4982篇 |
2007年 | 4680篇 |
2006年 | 4364篇 |
2005年 | 4096篇 |
2004年 | 3867篇 |
2003年 | 3985篇 |
2002年 | 3548篇 |
2001年 | 4150篇 |
2000年 | 4254篇 |
1999年 | 3373篇 |
1998年 | 1474篇 |
1997年 | 1388篇 |
1996年 | 1288篇 |
1995年 | 1530篇 |
1994年 | 1358篇 |
1993年 | 1232篇 |
1992年 | 2585篇 |
1991年 | 2700篇 |
1990年 | 2592篇 |
1989年 | 2611篇 |
1988年 | 2368篇 |
1987年 | 2348篇 |
1986年 | 2428篇 |
1985年 | 2340篇 |
1984年 | 1881篇 |
1983年 | 1677篇 |
1982年 | 1149篇 |
1979年 | 1749篇 |
1978年 | 1397篇 |
1977年 | 1151篇 |
1976年 | 1212篇 |
1975年 | 1292篇 |
1974年 | 1502篇 |
1973年 | 1516篇 |
1972年 | 1405篇 |
1971年 | 1304篇 |
1970年 | 1253篇 |
1969年 | 1283篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
911.
Serum samples of five goats inoculated with Toxoplasma gondii were analyzed using the enzyme linked immunosorbent assay (ELISA), indirect hemagglutination (IHA) and western blotting (WB). Antibodies detected by ELISA peaked between 19 and 62 days after inoculation and persisted throughout the experiment with no association to parasitaemia. Using WB, the main antigens detected had molecular weights of approximately 68, 62, 50, 48, 42, 34, 28, 26, 22 and 19 kDa. Antibody titers of between 1:256 and 1:32000 were observed using IHA, with a significant drop in activity after treatment with 2-mercapto-ethanol between days 12 and 48. This coincided with the parasitaemic period that occurs between 5 and 64 days after inoculation. 相似文献
912.
913.
914.
915.
Influence of Disease Process and Duration on Acute Phase Proteins in Serum and Peritoneal Fluid of Horses with Colic 下载免费PDF全文
T.H. Pihl E. Scheepers M. Sanz A. Goddard P. Page N. Toft P.H. Andersen S. Jacobsen 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(2):651-658
Background
The acute phase proteins (APP) serum amyloid A (SAA), haptoglobin, and fibrinogen are valuable blood biomarkers in equine inflammatory diseases, but knowledge of factors influencing their concentrations in blood and peritoneal fluid (PF) of horses with colic is needed.Objectives
The objective of this study was to investigate the influence of demographics (age, sex, breed), disease process (simple obstruction, strangulating obstruction, inflammatory), disease location, disease duration, hypovolemia, and admission hospital on concentrations of APP, lactate and white blood cell counts (WBC) in horses with colic admitted to 2 referral hospitals.Animals
The study included 367 horses with colic admitted at 2 referral hospitals.Methods
Prospective multicenter observational study of clinical data, as well as blood and PF biomarkers. Associations between biomarker concentrations and clinical variables were analyzed using multivariate linear regression analysis.Results
Increasing pre‐admission duration of colic was associated with increased concentrations of APP in blood and PF. Blood concentrations of SAA and fibrinogen were associated with disease process (inflammatory, strangulations, simple obstructions) in more colic duration groups (5–12 and >24 hours) than any of the other biomarkers. No relevant associations between demographic factors, hospital, or hydration status and the measured biomarkers were found.Conclusions and Clinical Importance
In horses with colic, concentrations of APP are associated mainly with disease process and duration of colic and may thus be used for assessment of disease independently of demographic or geographic factors. Serum amyloid A may be a diagnostic marker for use in colic differential diagnosis, but further evaluation is needed. 相似文献916.
M.S. Kraus J.D. Thomason T.L. Fallaw C.A. Calvert 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(1):1-6
Background: Asymptomatic Doberman Pinschers with dilated cardiomyopathy (DCM) often die suddenly owing to ventricular tachycardia that degenerates into ventricular fibrillation. A safe and effective antiarrhythmic drug treatment is needed. This will require a large, well-controlled, prospective study.
Hypothesis: Amiodarone toxicity is common in Dobermans with occult DCM and ventricular tachyarrhythmias refractory to antiarrhythmia therapy. Infrequent monitoring of hepatic function is inadequate. Frequent monitoring may be useful to determine dogs in which the dosage should be decreased or the drug withdrawn.
Methods: Medical records from the University of Georgia and Cornell University were searched for Doberman Pinschers diagnosed with preclinical DCM that received amiodarone for severe ventricular arrhythmias refractory to other antiarrhythmic agents. Echocardiographic data, Holter recording data, hepatic enzyme serum activity, and serum amiodarone concentrations were recorded. The presence of clinical signs of toxicity was recorded. Serum amiodarone concentrations were obtained in some dogs.
Results: Reversible toxicity was identified in 10 of 22 (45%) dogs.
Conclusion and Clinical Importance: Adverse effects from amiodarone were common and were, in part, dosage related. Patients should be monitored for signs of toxicity and liver enzyme activity should be measured at least monthly. 相似文献
Hypothesis: Amiodarone toxicity is common in Dobermans with occult DCM and ventricular tachyarrhythmias refractory to antiarrhythmia therapy. Infrequent monitoring of hepatic function is inadequate. Frequent monitoring may be useful to determine dogs in which the dosage should be decreased or the drug withdrawn.
Methods: Medical records from the University of Georgia and Cornell University were searched for Doberman Pinschers diagnosed with preclinical DCM that received amiodarone for severe ventricular arrhythmias refractory to other antiarrhythmic agents. Echocardiographic data, Holter recording data, hepatic enzyme serum activity, and serum amiodarone concentrations were recorded. The presence of clinical signs of toxicity was recorded. Serum amiodarone concentrations were obtained in some dogs.
Results: Reversible toxicity was identified in 10 of 22 (45%) dogs.
Conclusion and Clinical Importance: Adverse effects from amiodarone were common and were, in part, dosage related. Patients should be monitored for signs of toxicity and liver enzyme activity should be measured at least monthly. 相似文献
917.
Karen R. Humm MA VetMB DACVECC MRCVS ; Elizabeth A. Keenaghan-Clark MA VetMB MRCVS Amanda K. Boag MA VetMB DACVIM DACVECC FHEA MRCVS 《Journal of Veterinary Emergency and Critical Care》2009,19(4):352-356
Objective – To quantify the frequency of adverse events occurring during or post pericardiocentesis and to determine if adverse events are related to the cause of the pericardial effusion or frequency of pericardiocentesis.
Design – Retrospective study.
Setting – Referral hospital.
Animals, Intervention and Measurements – Medical records of 85 dogs that underwent 112 episodes of pericardiocentesis were reviewed. Any adverse events during pericardiocentesis and in the 48 hours post pericardiocentesis were noted. The frequency of adverse events was compared between dogs with a suspected neoplastic cause and a suspected nonneoplastic cause of their pericardial effusion and also between the first and subsequent pericardiocenteses.
Main Results – The incidence of adverse events was 10.7% within 1 hour of pericardiocentesis and 15.2% within 48 hours. There was no significant difference in the frequency of adverse events between the groups. Most adverse events identified were dysrhythmias. Forty-one percent of those dogs with adverse events were euthanized or died within 48 hours.
Conclusion – The incidence of adverse events seen within 48 hours of pericardiocentesis was 15.2%. 相似文献
Design – Retrospective study.
Setting – Referral hospital.
Animals, Intervention and Measurements – Medical records of 85 dogs that underwent 112 episodes of pericardiocentesis were reviewed. Any adverse events during pericardiocentesis and in the 48 hours post pericardiocentesis were noted. The frequency of adverse events was compared between dogs with a suspected neoplastic cause and a suspected nonneoplastic cause of their pericardial effusion and also between the first and subsequent pericardiocenteses.
Main Results – The incidence of adverse events was 10.7% within 1 hour of pericardiocentesis and 15.2% within 48 hours. There was no significant difference in the frequency of adverse events between the groups. Most adverse events identified were dysrhythmias. Forty-one percent of those dogs with adverse events were euthanized or died within 48 hours.
Conclusion – The incidence of adverse events seen within 48 hours of pericardiocentesis was 15.2%. 相似文献
918.
Three hundreds, 21 d-old slow-growing chicks were randomly divided among 5 treatments, of 5 replicates each. Each replicate
contained 12 unsexed chicks housed in (1 × 1) a floor pen. A group was kept under thermoneutral condition at 28 ± 4°C and
RH was 55 ± 3% during 21–84 d of age (positive control) and fed corn-soybean meal diet. The other four groups were kept for
three successive days per week under heat stress (HS) at 38 ± 1.4°C and 49 ± 2% RH from 12.00 to 16.00 pm. Chicks in HS treatments
were fed corn-soybean meal diet without (negative control) or with 250 mg AA/kg diet and Bet at 0.5 and 1 g/kg diet. HS decreased
productive performance, increased (P < 0.05) meat dry matter, plasma triglyceride and serum calcium whereas decreased (P > 0.05) plasma glucose, serum total protein and water holding capacity (WHC) of meat. AA and 1 g of Bet/kg diet was equally
potent for partial relief (P < 0.05) of the negative effect of HS on growth, increased (P < 0.05) feed intake, protein digestibility (P < 0.05), dressing out percentage, liver and giblets, whilst improved (P < 0.05) feed conversion ratio (FCR). Also, a complete recovery from the negative effect (P < 0.05) of HS shown on plasma glucose and partial recovery (P < 0.05) observed in total protein, triglyceride, blood pH, packed cell volume (PCV), hemoglobin (Hgb), rectal temperature
(RT) and respiration rate (RR) and improved humoral immune competence to sheep red blood cell (SBRCs) test. 相似文献
919.
L J Saif K L Smith B J Landmeier E H Bohl K W Theil D A Todhunter 《American journal of veterinary research》1984,45(1):49-58
Fifteen pregnant Holstein cows were freely assigned to 3 experimental groups (5 cows in each group). Cows in group I were inoculated IM and intramammarily (IMm) with Ohio Agricultural Research and Development Center (OARDC) tissue culture-propagated modified-live Nebraska calf diarrhea bovine rotavirus with added adjuvant (OARDC vaccine-immunized cows). Group II cows were given IM injections of a commercial modified-live rotavirus-coronavirus vaccine (commercial vaccine-immunized cows), and the remaining 5 cows were noninoculated controls (group III). Rotavirus antibody in colostrum and milk was mainly associated with immunoglobulin (Ig)G1, and less so with IgG2, IgA, and IgM, as analyzed by the enzyme-linked immunosorbent assay (ELISA), using monospecific anti-bovine IgG1, IgG2, IgM, and IgA sera. In serum, the rotavirus antibody was distributed almost equally between IgG1 and IgG2. The same relationships appeared in both immunized and nonvaccinated cows. All OARDC vaccine-injected cows had virus-neutralization (VN) and ELISA IgG1 rotavirus antibody titers in serum and mammary secretions at significantly increased levels (at least 100-fold; P less than 0.05) compared with the titers in groups II (commercial vaccine-immunized cows) and III (controls). Serum, colostrum, and milk antibody titers from these latter 2 groups did not differ statistically. The ELISA IgG2, IgA, and IgM rotavirus antibody titers also were significantly greater in mammary secretions from OARDC vaccine-immunized cows than in groups II and III cows. There was a high correlation between ELISA IgG1 and VN rotavirus antibody titers for all samples tested (r = 0.97, P less than 0.001), but ELISA IgG1 antibody titers were consistently higher than VN titers. The ELISA IgG1 and VN antibody titers of milk samples collected from cows 30 days after parturition were higher from the OARDC vaccine-immunized cows (ELISA IgG1, geometric mean titer (GMT) = 3,511; VN GMT = 1,689) than were titers from the group II cows (ELISA IgG1 GMT = 39; VN GMT = 33) or group III cows (ELISA IgG1 GMT = 21; VN GMT = 19). These results indicate that IM plus IMm immunization of pregnant cows, using modified-live bovine rotavirus with added adjuvant, may significantly enhance serum, colostrum, and milk rotavirus antibody titers, whereas IM vaccinal inoculation of pregnant cows with a commercial modified-live rotavirus-coronavirus vaccine may not. 相似文献
920.
1. Under summer conditions in north Iraq, 2 000 chicks were reared for 56 d in two broiler houses. One house was provided with two air‐coolers. The birds were housed at densities of eight to 16 birds/m2.
2. Differences in weight gain between birds in cooled and uncooled houses ranged from 101.5 g at eight birds/m2 to 385 g at 16 birds/m2. Food consumption increased gradually with increasing bird density. Food conversion efficiency, meat yield and carcass conformation were much better in the cooled house than under conventional conditions.
3. The financial return/m2 ranged from 2.30 to 4.51 ID1 in the cooled house, compared with 2.01 to 2.77 ID in the uncooled house at different floor densities. 相似文献