杀虫剂新产品田间药效试验评价概述

本文梳理了2015年~2017年开展田间小区药效试验的18个含新有效成分的杀虫剂产品,其中双酰胺类6个、新烟碱类3个、介离子类2个、杀螞剂3个、其它类型4个。在9大类作物的14种害虫上进行了田间药效试验,本文归纳了上述新杀虫剂有效成分的作用机理及田间药效试验情况。     

植物病害生防因子的作用机制及应用进展

应用植物病害生防因子是解决化学农药3R问题、促进农业绿色发展的有效途径。本文概述了拮抗微生物、抗生素、植物诱导子等生防因子控制植物病害的作用机制和应用研究进展,分析了生防因子应用存在的问题,并对植物病害生物防治的未来发展方向进行了展望。     

F-box domain is involved in regulating the proliferation of MCF-7 cells by FBXO39 protein

AIM: To investigate the effect of F-box domain on the regulation of MCF-7 cell proliferation by FBXO39 protein. METHODS: The effect of F-box domain on the localization of FBXO39 protein in the MCF-7 cells was investigated. MCF-7 cell cDNA library was used as the template resource. The full-length cDNA sequence of FBXO39 was amplified by PCR method and subcloned into eukaryotic expression vector pEGFP-C2. The pEGFP-FBXO39ΔF (F-box domain deletion mutation) plasmid was successfully constructed with the template resource of pEGFP-FBXO39 plasmid. The recombinant plasmids were transfected into the MCF-7 cells, and then the expression of FBXO39 and FBXO39ΔF were determined by Western blot. The cellular localization of FBXO39 and FBXO39ΔF were observed by confocal microscopy. The localization of endogenous FBXO39 in the MCF-7 cells was detected by immunofluorescence staining. In addition, MTT and EdU assays were used to measure the cell proliferation, flow cytometry was used to measure the cell cycle distribution, and immunohistochemical staining was used to observe the expression of FBXO39 in the breast cancer and para-carcinoma tissues. RESULTS: The eukaryotic expression vector pEGFP-FBXO39 and pEGFP-FBXO39ΔF were constructed successfully. F-box domain had no effect on the cell localization of FBXO39. FBXO39 promoted MCF-7 cell proliferation but FBXO39ΔF did not. FBXO39 was highly expressed in the breast cancer tissues. CONCLUSION: F-box domain had no effect on the cellular localization of FBXO39 protein. However, it plays an important role in the biological function of FBXO39. FBXO39 may be related to breast cancer tumorigenesis.     

小型电磁振动式小麦播种机的工作原理与结构分析

针对传统小型小麦播种机的缺点,设计了一种播种质量高、速度快、安全可靠、播种成本低、适应不同田间要求、采用电磁振动播种并能有效减少能耗的小型电磁振动式小麦播种机。该机田间作业时,可以将开沟、播种、平土一次完成,大大简化了小麦播种时的生产流程,有利于进一步提高播种效率,解放生产力。为此,介绍了小型电磁振动式小麦播种机的整体构造,分析了小型电磁振动式小麦播种机的工作原理,对配套部分及重要零件利用inventor自带应力分析模块建立了有限元模型。有限元分析的结果表明:小型电磁振动式小麦播种机能够满足工作要求,且各零件满足刚度和强度的要求。经过进一步的改进和研究,此机将在播种小麦方面有较大的发展前景,同时对各种低能耗播种机的研制也具有一定的参考作用。     

铧式犁减阻性能研究

优化铧式犁犁体曲面形状,降低耕作阻力,是一项亟待解决的问题。从犁体曲面成型方法可知,导曲线形状是影响铧式犁耕作阻力的一个重要因素。为此,以BTU35犁体为基础,在Solid Works中建立5种不同导曲线犁体,并将模型导入ANSYS进行显示动力学分析,得到其耕作阻力。仿真试验表明:以四次多项式曲线为导曲线形成的犁体耕作阻力较小,在3km/h时降阻3.75%,7km/h时降阻4.56%,平均降阻3.93%。结合不同导曲线曲率半径变化情况与工作阻力之间的关系,可得结论:导曲线曲率半径变化复杂的犁体有较好的减阻性能。     

High-throughput phenotyping:Breaking through the bottleneck in future crop breeding

With the rapid development of genetic analysis techniques and crop population size, phenotyping has become the bottleneck restricting crop breeding. Breaking th...     

群居型和散居型亚洲小车蝗成虫能量代谢相关酶活性测定

为明确亚洲小车蝗Oedaleus asiaticus成虫飞行肌对其体内能源物质利用的情况,采用室内生化法测定能源物质代谢相关5种酶,即3-磷酸甘油醛脱氢酶(glyceraldehyde phosphate dehydrogenase,GAPDH)、3-磷酸甘油脱氢酶(glycerol-3-phosphate dehyd...     

陆地棉转录因子GhNAC78基因的特征及功能分析

本实验室从陆地棉中克隆得到GhNAC78,分析了其基因特征及功能。该基因cDNA全长为937 bp,开放阅读框为876bp,编码291个氨基酸,隶属于NAM转录因子亚家族。体外转录激活实验表明,GhNAC78具有转录激活活性。上游启动子区1537 bp序列的生物信息学分析可知其包含多种激素、胁迫和光响应元件,表明GhNAC78广泛地参与植物生长发育和胁迫响应的调控。荧光定量结果显示,GhNAC78在棉花叶片衰老过程中高调表达,推测其参与叶片衰老的调控。     

汽车车身导水主板导水槽结构设计

讲述了两种不同的车身前部导水槽结构,封闭式导水槽和单板式导水槽结构,并分别对两种导水槽的导水性、安装要求、焊接工艺性以及和周边零件搭接关系等技术问题进行了说明。并以某款汽车的前舱导水槽结构为例,展开讲述了汽车前舱导水主板总成中导水槽的结构特点和种类,以及在设计过程中需要注意的关键点。且列举了几种常用的导水槽结构,为导水槽设计时提供参考。