利用谷氨酰胺转氨酶和结冷胶提高鱼丸凝胶强度的研究

用磷酸盐提高鱼丸凝胶强度的效果显著,但根据GB2760规定,用量有限。采用谷氨酰胺转氨酶(TG酶)和结冷胶替代磷酸盐,优化配方为:谷氨酰胺转氨酶添加量为0.4%,结冷胶添加量为0.5%,产品的凝胶强度达到使用磷酸盐的1.9倍。     

新型农药对抗虫棉田棉蚜防治效果研究

在转Bt基因抗虫棉田,对8种防治棉蚜的新型农药进行了药效试验.结果表明,莫比朗、吡虫啉对棉蚜防效较好,施药后1~10 d的防效分别为90.9%~98.7%和90.9%~94.6%,其中莫比朗的防治效果最好,不但活性高,见效快,且持效期长,有效控制期达15 d以上;好年冬、丙溴磷和高渗吡虫啉也表现出较好的防治效果;氧乐果、氯氰菊酯防效较差.     

包衣尿素在春白菜上的应用效果研究

以枣优良品种骏枣为试材,研究了多种因素对其离体叶片及大田幼嫩茎段再生效率的影响。结果表明,幼嫩茎段再生较容易。叶片再生与生长调节物质种类和浓度及叶片成熟度有关。枣叶片不定芽再生的最佳培养基为1/2MS(大量元素减半) TDZ0.5mg/L IBA0.2mg/L。不定芽再生率以上部叶片最高。     

PKS-NRPS数据库的研究进展

非核糖体多肽合成酶(NRPS)和聚酮合成酶(PKS)是调控次级代谢物聚酮类物质和非核糖体多肽合成的关键酶。随着生物息学的快速发展,国外的NRPS-PKS数据库和预测软件不断推出。本文对几个重要NRPS和PKS领域的数据库与预测软件的研究做一综述     

广东省6月玉米、豆粕、进口鱼粉价格分析

参考广州兆华金丰农牧实业有限公司提供的“广东省6月份部分鸡、鸭、猪、鱼价格分析”报告（表1）对比,广东5月份鸡价明显上升,达到盈利状态;鸭价、猪价平稳,处于盈、亏之间;鱼价略跌。6月份鸡价、鸭价将会稳中有涨;猪价仍以平稳为主：根据罗非鱼、四大家鱼的存塘量和市场销售情况来看。鱼价将可能继续下调。     

甘肃不同地区油松针叶氨基酸成分分析研究

油松（Pinus tabulaeformis Carr．）是近年来引入干旱地区的主要造林树种,目前甘肃省已建立万亩油松苗育基地,进行大面积示范推广,主要分布于陇东、陇南、天水等地区。油松的饲用价值和药用价值是针叶,针叶富含多种营养成分,可制成松针精油、松针软膏、松针粉、抗癌扶正冲剂、松针饮料等产品,特别是以石油醚脱脂后的松针用乙醇提取混合氨基酸,作为饲料添加剂,可以促进动物生长,增强抗病能力。但其地域不同,营养成分含量也不尽相同。     

采用PCR-SSCP技术分析蚯蚓粪便的微生物群落结构

通过SDS/蛋白酶的方法抽提蚯蚓粪便微生物总DNA,以总DNA为模板,采用通用引物分别成功扩增细菌16S rRNA基因的V4-V5可变区,真菌18S rRNA基因的V8-V9可变区,应用单链构象多态性技术（SSCP）分析了蚯蚓粪便中不同空间层次的微生物种群的多样性。结果表明,在粪便的不同空间层次上呈现出明显的空间分布多样性,并且与纯蚯蚓粪微生物种群的多样性有很大的差别。为进一步的蚯蚓粪生态学功能研究提供了有益的指导。     

Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

硫酸新霉素可溶性粉在靶动物猪的安全性研究

为了客观评价硫酸新霉素可溶性粉对靶动物猪临床应用的安全性,将32头仔猪随机分为4组,每组8头,分别设1倍、3倍、5倍临床推荐剂量组和空白对照组,连续混饮硫酸新霉素可溶性粉15 d,通过检测各组受试猪的临床表现、血液常规指标、血清生化指标、尿液常规指标及病理学变化等评价其临床用药的安全性。结果:硫酸新霉素可溶性粉以5倍临床推荐剂量内应用于靶动物猪后,对其临床症状、体重、血常规、血生化及尿常规等指标没有显著影响,解剖学及病理组织学检查也未见任何病理变化,说明硫酸新霉素可溶性粉以1倍、3倍、5倍临床推荐剂量应用于靶动物猪是安全的。     

不同品系鸡血浆转铁蛋白、血红蛋白和红细胞酯酶D多态性研究

采用聚丙烯酰胺凝胶等电聚焦电泳法测定了6个品系鸡的血浆转铁蛋白(Tf)、血红蛋白(Hb)和红细胞酯酶D(EsD)的遗传多态性.转铁蛋白出现5种表型,受Tf~A、Tf~B、TP~C3个共显性等位基因控制;血红蛋白出现5种表型,受Hb~F、Hb~(M1)、Hb~(M2)3个共显性等位基因控制;红细胞酯酶D出现5种表型,受EsD~1、EsD~2、EsD~3、EsD~4、EsD~55个共显性等位基因控制.各品系鸡之间的Tf、Hb、EsD的基因频率是不同的.根据基因频率计算遗传距离并用类平均法进行聚类分析.3种肉用鸡中艾维茵和塔特姆亲缘关系最近,罗斯褐鸡与3种肉用鸡亲缘关系较近,而与其它蛋用型鸡亲缘关系较远.