The ameliorative potential and antioxidant capacity of an extract of Urtica dioica seeds (UDS) was investigated using histopathological changes in liver and kidney, measuring serum marker enzymes, antioxidant defence systems and lipid peroxidation (malondialdehyde (MDA)) content in various tissues of broilers exposed to aflatoxin (AF).
A total of 32 broilers were divided randomly into 4 groups: control, UDS extract-treated, AF-treated and AF+UDS extract-treated. Broilers in control and UDS extract-treated groups were fed on a diet without AF. The AF-treated group and AF+UDS extract-treated groups were treated with an estimated 1 mg total AF/kg feed. The AF+UDS extract groups received in addition 30 ml UDS extract/kg diet for 21 d.
The AF-treated group had significantly decreased body weight gain when compared to the other groups.
Biochemical analysis showed a small increase in the concentrations of serum aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transpeptidase and lactate dehydrogenase in the AF-treated group compared to that of the control group, whereas concentrations of these enzymes were decreased in the AF+UDS group compared to that of the AF-treated group.
Administration of supplementary UDS extract helped restore the AF-induced increase in MDA and reduced the antioxidant system towards normality, particularly in the liver, brain, kidney and heart. Hepatorenal protection by UDS extracts was further supported by the almost normal histology in AF+UDS extract-treated group as compared to the degenerative changes in the AF-treated broilers.
It was concluded that UDS extract has a protective hepatorenal effect in broilers affected by aflatoxicosis, probably acting by promoting the antioxidative defence systems.
In this study, we aimed to investigate and compare the direct toxic and teratogenic effects of dimenhydrinate, metoclopramide and trimethobenzamide HCl, antiemetic drugs on embryonic growth and development in cultured rat embryos. Embryos were explanted on day 9.5 of gestation and cultured. Whole rat serum was used as a culture medium for the control group while different concentrations of dimenhydrinate (2.5–20 μg/ml), metoclopramide (10–50 μg/ml) and trimethobenzamide HCl (25–100 μg/ml) were added to serum for the experimental groups. Effects of antiemetics on embryonic developmental parameters were compared, and embryos were evaluated for the presence of any malformations. Also, the total DNA was extracted from the cells to determine the fragmentation of nuclear DNA of embryonic cells. Compared with the control embryos, the antiemetics significantly decreased all growth and developmental parameters dose dependently. There was no difference regarding the fragmentation of nuclear DNA of the all used agents and controls. Amongst the agents, trimethobenzamide HCl was found to have more toxic and teratogenic potential, and metoclopramide appears to be the least toxic antiemetic and therefore could be more safely used and might be preferred for the treatment of nausea and vomiting in pregnancy. 相似文献
Tropical Animal Health and Production - The present study reports the molecular and antigenic characterization of 13 bovine herpesvirus type 1 (BoHV-1) field viruses obtained from cattle with... 相似文献
Use of reduced and no-tillage systems has increased in recent years due to concerns for ecological and economic sustainability of agricultural production. Effective weed control is a serious concern in reduced tillage production. This study was conducted to investigate weed control practices in reduced and no-till maize production. The most effective timing of glyphosate application, either before or after sowing, was investigated in combination with pre-emergence application of acetochlor (840 g a.i/L), post-emergence application of foramsulfuron (22.5 g a.i/L), and two hoeing treatments. The treatments were maintained on the same plots during 2011 and 2012 to evaluate the cumulative effects of the treatments. Main plot treatments consisted of four timings of glyphosate application: 20 or 10 days before sowing, day of sowing, 5 days after sowing, and an untreated control. Sub-plot treatments were: pre- plus post-emergence herbicides, pre-emergence herbicide plus rotary hoeing, post-emergence herbicide plus rotary hoeing, and post-emergence herbicide plus two hoeing treatments (rotary and lister hoe). In the main plots, the lowest weed biomass was produced in glyphosate treatments at sowing and 5 days after sowing; the highest biomass was produced in control plots and in the plots with glyphosate treatments 20 days before sowing. In the sub-plots, the greatest weed biomass was produced in plots with two hoeing treatments (rotary and lister hoe). Glyphosate treatments at sowing and post-emergence herbicide treatment combinations produced the best weed control. Economic analysis revealed that pre-sowing, non-selective herbicide treatments provided a slight increase in net profit. Mechanical hoeing decreased net income due to increased production costs. The highest income was obtained from the pre-emergence plus post-emergence herbicide treatment combinations with no glyphosate. 相似文献
Over the last two decades, the demand for organic products has grown rapidly in the world due to increased concern about side
effects of pesticides on the environment and human health. Studies were conducted in organic lettuce (Lactuca sativa L.) from 2004 to 2005 at the Black Sea Agricultural Research Institute in Samsun, Turkey, to determine the suppressive effects
of summer cover crops on weeds. The experiment was arranged in a randomized complete block design with four replications.
Treatments consisted of grain sorghum [Sorghum bicolor (L.) Moench.], sudangrass [Sorghum vulgare Pers. var. sudanense (Piper) Hitchc.], hairy vetch (Vicia villosa Roth.), grain amaranth (Amaranthus cruentus L.), pea (Pisum sativum L.) and bare ground with no cover crop. Weed density and total weed dry biomass were assessed before and at 14, 28, and 56 days
after incorporation (DAI) of the cover crops. The cover crops produced between 1.2 and 3 t ha−1 biomass and grain sorghum produced more dry matter than any other species in both years. After incorporation of the cover
crops, hairy vetch and sorghum treatments showed fewer weed species, and lower weed density than the other cover crops in
both years. Hairy vetch, grain sorghum, and sudangrass were the most effective cover crops and reduced total weed dry biomass
by 90.3%, 87.4%, and 86.9% in 2004, and by 88%, 86.3%, and 85.2% in 2005, respectively. Cover crop residue suppressed many
broadleaved weed species but failed to control grass weeds. Hairy vetch treatments produced the highest yield, followed by
sudangrass and grain sorghum. Yields with grain amaranth and pea were similar to that of the control. These results indicate
that hairy vetch, grain sorghum, and sudangrass can be used to suppress weeds in early season of organic lettuce production. 相似文献
Research on mastitis in dairy cows caused by Escherichia coli has reported the emergence of strains capable of inducing chronic mastitis and that these strains adhered to and internalized
into bovine mammary epithelial cells better than strains of E. coli isolated from acute mastitis. To understand mechanisms and strategies used by chronic E. coli strains to survive intracellularly internalization studies using bovine mammary epithelial cells treated with inhibitors
of caveolae-mediated endocytosis (CME) and receptor-mediated endocytosis (RME), double immunofluorescence labeling confocal
laser and fluorescence microscopy were conducted. Internalization studies showed that strains chronic E. coli strains persisted intracellularly longer than acute E. coli strains. Treatment of bovine mammary epithelial cells CME or RME inhibitors resulted in lower numbers of intracellular E. coli strains associated with chronic or acute mastitis than untreated controls. In addition, when selective CME inhibitors were
used significantly fewer chronic E. coli were detected intracellularly than acute E. coli or untreated controls. Confocal laser microscopy showed that chronic E. coli strains colocalized preferentially with caveolae whereas acute strains did so with early endosomes, an early step of RME.
These results suggest that strains of E. coli associated with chronic mastitis exploit lipid rafts/CME to internalize into and move through mammary epithelial cells. By
exploiting this endocytosis pathway, chronic E. coli strains avoid bactericidal mechanisms such as endosome acidification and endosome-lysosome fusion, thus allowing intracellular
survival. Data from this study helps to explain how these strains are capable of causing chronic E. coli mastitis. 相似文献
Escherichia coli infection is one of the most common causes of bovine mastitis in well managed dairies. Although E. coli infections are usually transient, E. coli can also cause persistent intramammary infections. We sought to determine whether E. coli isolates recovered from either transient or persistent intramammary infections differed both genetically and in their ability to invade mammary epithelial cells. E. coli isolates from transient (EC(trans), n=16) and persistent (EC(pers), n=12) mastitis cases were compared for differences in overall genotype, virulence genes, serotype, phylogroup (A, B1, B2, D), and invasion of bovine mammary epithelial cells, MAC-T by microarray analysis, suppressive subtractive hybridization, PCR and gentamicin protection assays. EC(trans) and EC(pers) were diverse in overall genotype and serotype, and were predominantly of phylogroups A and B1. Both EC(trans) and EC(pers) contained genes encoding type II, IV and VI secretion systems, long polar fimbriae (lpfA) and iron acquisition, and lacked genes associated with virulence in diarrheagenic E. coli. EC(trans) had fewer virulence genes than EC(pers) (p<0.05), but no individual virulence genes were unique to either group. In phylogroup A, EC(pers) were more invasive than EC(trans) (p<0.05), but no difference was observed between them in phylogroup B1. Enhanced epithelial cell invasion was associated with lpfA (p<0.05). Our findings indicate that a genetically diverse group of E. coli is associated with transient and persistent mastitis. We did not identify a set of bacterial genes to account for phenotypic differences. However, we found that mastitis phenotype, phylogroup and presence of lpfA were associated with the ability to invade cultured bovine mammary epithelial cells. 相似文献