全文获取类型
收费全文 | 1228篇 |
免费 | 214篇 |
国内免费 | 38篇 |
专业分类
林业 | 168篇 |
农学 | 105篇 |
基础科学 | 39篇 |
141篇 | |
综合类 | 458篇 |
农作物 | 127篇 |
水产渔业 | 77篇 |
畜牧兽医 | 261篇 |
园艺 | 49篇 |
植物保护 | 55篇 |
出版年
2024年 | 19篇 |
2023年 | 8篇 |
2022年 | 36篇 |
2021年 | 43篇 |
2020年 | 65篇 |
2019年 | 49篇 |
2018年 | 24篇 |
2017年 | 31篇 |
2016年 | 46篇 |
2015年 | 47篇 |
2014年 | 53篇 |
2013年 | 86篇 |
2012年 | 95篇 |
2011年 | 101篇 |
2010年 | 64篇 |
2009年 | 59篇 |
2008年 | 58篇 |
2007年 | 62篇 |
2006年 | 53篇 |
2005年 | 30篇 |
2004年 | 30篇 |
2003年 | 65篇 |
2002年 | 109篇 |
2001年 | 101篇 |
2000年 | 48篇 |
1999年 | 30篇 |
1998年 | 6篇 |
1997年 | 8篇 |
1996年 | 6篇 |
1995年 | 8篇 |
1994年 | 7篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1965年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有1480条查询结果,搜索用时 20 毫秒
11.
12.
Constança S. P. Camilo-Alves Margarida Vaz M. Ivone Esteves Da Clara Nuno Manuel De Almeida Ribeiro 《New Forests》2017,48(6):753-772
Chronic decline and Sudden death are two syndromes of cork oak (Quercus suber) dieback. Mortality is associated with water stress, but underlying physiological mechanisms are poorly understood. Here, we investigated the physiological performance of declining trees during the summer drought. Leaf water potential, gas-exchange, fluorescence of photosystem II and leaf and root starch concentration were compared in healthy (asymptomatic) and declining trees. Low annual cork increment in declining trees indicated tree decline for several years. All trees showed similar water status in spring. In summer, declining trees showed lower predawn leaf water potential (?2.0 vs. ?0.8 MPa), but unexpectedly higher midday leaf water potential than healthy trees (?2.8 vs. ?3.3 MPa). The higher midday water potential was linked to by means of strongly reduced stomatal conductance and, consequently, transpiration. This study is pioneer showing that declining trees had high midday water potential. A tendency for lower sap flow driving force (the difference between predawn and midday water potential) in declining trees was also associated with reduced photosynthesis, suggesting that chronic dieback may be associated with low carbon uptake. However, starch in roots and leaves was very low and not correlated to the health status of trees. Declining trees showed lower water-use efficiency and non-photochemical quenching in summer, indicating less resistance to drought. Contrarily to chronic decline, one tree that underwent sudden death presented predawn leaf water potential below the cavitation threshold. 相似文献
13.
根据多年定位测定的数据以及<湖南省森林资源主要数据汇编>、<湖南省农业区划(8)>和湖南省水利厅有关资料,对湖南省森林的木材生产效益和水源涵养效益进行计量评估,结果表明,湖南省现有的森林每年生长木材488.7675万m3,年平均生产木材效益24.44亿圆,而其森林的水源涵养效益中,森林贮水功能效益9.84亿圆,防洪效益3.68亿圆,枯水期增加的水资源利用效益26.73亿圆.其涵养水源总效益共计40.25亿圆,是木材效益的1.65倍,同时,即使是灌草坡地调节水分的能力也明显高于裸地,因此.从水文学观点出发,对于难以造林的山坡、山顶,要注意灌木林的保护和经营,使其发挥更好的防水功能. 相似文献
14.
The effects of nitrogen (N‐) and phosphorus (P‐) deficiency, isolatedly or in combination, on growth, nitrogenous fraction, and inorganic phosphate in xylem exudade, and photosynthesis of common bean (Phaseolus vulgaris L. cv. Negrito) were investigated. Plants were grown in nutrient solution adjusted daily to pH 5.5 and aerated continuously. Ten days after emergence mineral deficiency was imposed. Plants were then supplied with high N (7.5 mol m‐3) or low N (0.5 mol m‐3), and also with high P (0.5 mol m‐3) or low P (0.005 mol m‐3). All sampling and measurements were made 28 days after emergence. N‐ or P‐deprivation brought about large decreases in total leaf area by inhibiting the emergence of new leaves and primarily the expansion of the leaves. The specific leaf area did not change under N‐ but decreased under P‐limitation. The decreased shoot to root ratio in all deficiency treatments was a consequence of a lowering mass of above‐ground organs, especially of leaves. The content of chlorophylls declined significantly only under N‐deficiency alone; carotenoids declined under both N‐ and combined N‐ and P‐limitation. No alteration in amino acid concentration in xylem exudate occurred in plants experiencing N‐starvation, while ureides increased by 79%, and nitrate and inorganic phosphate decreased greatly. Under P‐deprivation, amino acids and nitrate in xylem sap dropped by about half; ureides were held relatively constant, and phosphate was severely depressed. Total upward translocation of N through xylem was estimated to be about 16% higher in N‐deficient plants than in plants without mineral limitation, but leaf N levels in the former were lower as compared to control plants. The net carbon (C) assimilation decreased similarly regardless of the imposed deficiency treatment. Such a decrease was mainly determined by non‐stomatal factors. In general, no additive effect between N‐ and P‐limitation on any of measured parameters was observed. 相似文献
15.
稻、鱼、鸭、菜复合生态模式的建立与应用 总被引:4,自引:0,他引:4
1998-1999年在贵州省石阡县进行了稻、鱼、鸭、菜复 生态模式示范,示范面积12hm^2,获得了平均产鲜鱼750kg/hm^2、瓜果1650kg/hm^2、成鸭225只/hm^2,增产水稻750kg/hm^2的良好效益。本文阐述了“稻、鱼、鸭、菜”多元复合系统的生态学原理及生产技术要点。 相似文献
16.
Gomes Pöppl Á Costa Valle S Hilário Díaz González F de Castro Beck CA Kucharski LC Silveira Martins Da Silva R 《Veterinary research communications》2012,36(1):81-84
Estrus cycle is a well recognized cause of insulin resistance in bitches. The insulin receptor (IR) as well as the insulin-like growth factor-I receptor belong to the same subfamily of tyrosine kinase (TK) receptors. The objective of this study was to evaluate basal TK activity in muscle tissue of bitches during the estrus cycle. Twenty-four bitches were used in the study (7 in anestrus, 7 in estrus, and 10 in diestrus). Muscle samples, taken after spaying surgery to determine TK activity, were immediately frozen in liquid nitrogen and then stored at −80°C until the membranes were prepared by sequential centrifugation after being homogenized. TK activity was determined by Poly (Glu 4:Tyr 1) phosphorylation and expressed in cpm/μg of protein. TK activity was significantly lower (P < 0.001) in the animals in estrus (104.5 ± 11.9 cpm/μg of protein) and diestrus (94.5 ± 16.9 cpm/μg of protein) when compared with bitches in anestrus (183.2 ± 39.2 cpm/μg of protein). These results demonstrate, for the first time, lower basal TK activity in the muscle tissue of female dogs during estrus and diestrus, which may represent lower insulin signaling capacity, opening a new field of investigation into the molecular mechanisms of insulin resistance in dogs. 相似文献
17.
Méndez D Giménez F Escalona A Da Mata O González A Takiff H de Waard JH 《Veterinary microbiology》2006,116(4):325-328
The ability of Mycobacterium paratuberculosis to survive the commercial pasteurization process of raw milk remains controversial. In a study undertaken in Venezuela to culture M. paratuberculosis from commercially pasteurized cows' milk, 83-200 ml containers of milk were processed and cultured on Herrold's egg yolk slants. No M. paratuberculosis was cultured but a total of six colonies of Mycobacterium bovis were isolated from one container each from two different milk providers. Because laboratory cross-contamination was suspected, the laboratory records were reviewed and spoligotyping was carried out on the isolated individual colonies. On the day before these milk specimens were processed, the biological safety cabinet had been used for the isolation of M. bovis from lymph nodes from infected cattle. Spoligotyping showed that that the colonies isolated from the milk all had the same pattern as the strains isolated from the lymph nodes that were processed the previous day. As far as we know, this is the first report of cross-contamination in a veterinary mycobacterial laboratory. False-positive cultures in the mycobacterial laboratory are not rare. In this setting M. bovis was isolated because it is the most common manipulated organism in this laboratory. We believe that reports on the isolation of M. paratuberculosis from commercially pasteurized milk should exclude cross-contamination before reporting, especially when this organism is routinely isolated from animal material in the same lab. 相似文献
18.
F Bernard R Furneaux C Adrega Da Silva J-F Bardet 《Veterinary and comparative orthopaedics and traumatology》2008,21(1):64-68
rhBMP-2 solution on a collagen sponge was placed along the diaphysis of an atrophicradius, which had a history of recurring fractures. Two months after rhBMP-2 treatment, new mineralized bone was present, which significantly increased the diameter of the radius and allowed the removal of the external skeletal fixator (ESF). Due to carpo-metacarpal joint compromise, a pancarpal arthrodesis was performed seven months later. At follow-up evaluation two years later the dog was only very mildly lame. 相似文献
19.
A method based on direct and indirect counting is developed for rapid and accurate linkage analysis for codominant and dominant loci. Methods for estimating gender-specific recombination frequencies are available for cases where at least one of the two loci is multiallelic and for biallelic loci with mixed parental linkage phases where at least one locus is codominant. Most of the estimates of gender-average and gender-specific recombination frequencies required iterative solutions. The new method makes use of the full data set, yields exact estimates of the recombination frequencies when the observed and expected genotypic frequencies are equal, and are computationally efficient. Relative efficiency of various data types is affected by the inheritance mode and by parental linkage phases of biallelic loci, but unaffected by the locus polymorphism when using the full data set for linkage analysis. The ability to determine parental linkage phases is affected by the locus polymorphism as well as inheritance mode. Intercross (or F-2 design) is more efficient for mapping codominant loci, whereas backcross is more efficient if dominance is involved. Mixed parental linkage phases of biallelic loci are less efficient than coupling or repulsion linkage phases. Ignoring noninformative offspring results in biased estimates of recombination frequency for biallelic loci only and reduced LOD scores for all cases. 相似文献
20.
Thiago Luiz Da Mata Monique Inês SegerenAline Segeren Fonseca Carlos Augusto Colombo 《Scientia Horticulturae》2009
Genetic diversity was evaluated by RAPD markers and morpho-agronomic characters for a total of 42 accessions of Barberton daisy (Gerbera jamesonii) consisting of 29 commercial and 13 wild accessions. A total of 74 polymorphic bands were obtained employing a set of 12 primer pairs. The average genetic similarity coefficient for the 42 accessions, evaluated by Jaccard index was 0.55 ranging from 0.28 to 1.00. The genetic structure found among Barberton daisy accessions was evaluated by hierarchic classification analyses and UPGMA modeling, revealing six clusters of genotypes where two of them include the wild accessions and the remaining four including commercial material, except for wild genotype number 9. Shannon (H′) index was calculated using the molecular markers to investigate the genetic variation among the Gerbera accessions and showed higher values for the commercial cluster in comparison to the values obtained for the individuals from the non-commercial cluster, namely 0.34 versus 0.27, respectively. Therefore, both calculated indices (Jaccard and Shannon) indicated the presence of higher genetic variation among commercial accessions in comparison to the cluster representing non-commercial accessions, suggesting that genetic breeding programs may focus on commercial accessions to recombine interesting genotypes with commercially important and marketing-desired characteristics. 相似文献