全文获取类型
收费全文 | 1700篇 |
免费 | 130篇 |
专业分类
林业 | 79篇 |
农学 | 31篇 |
基础科学 | 4篇 |
350篇 | |
综合类 | 165篇 |
农作物 | 87篇 |
水产渔业 | 136篇 |
畜牧兽医 | 804篇 |
园艺 | 60篇 |
植物保护 | 114篇 |
出版年
2024年 | 11篇 |
2023年 | 36篇 |
2022年 | 30篇 |
2021年 | 73篇 |
2020年 | 86篇 |
2019年 | 81篇 |
2018年 | 56篇 |
2017年 | 56篇 |
2016年 | 68篇 |
2015年 | 62篇 |
2014年 | 67篇 |
2013年 | 68篇 |
2012年 | 142篇 |
2011年 | 151篇 |
2010年 | 77篇 |
2009年 | 69篇 |
2008年 | 125篇 |
2007年 | 110篇 |
2006年 | 91篇 |
2005年 | 72篇 |
2004年 | 68篇 |
2003年 | 70篇 |
2002年 | 67篇 |
2001年 | 4篇 |
2000年 | 10篇 |
1999年 | 7篇 |
1998年 | 8篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 7篇 |
1993年 | 11篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有1830条查询结果,搜索用时 15 毫秒
41.
Tovar L Salafranca J Sánchez C Nerín C 《Journal of agricultural and food chemistry》2005,53(13):5270-5275
Both specific and overall migration tests have been applied to new experimental food packaging-active plastic films with antioxidant properties, including in its composition a natural rosemary extract. Determination of volatile and semivolatile compounds migrating from plastic to the four established simulants showed that both specific and overall migration was very low. The results obtained gave values 20 times lower than the established limits in the worst case. So, from the point of view of health risk, the new active packaging can be considered as safe. Analytical procedure used provided the necessary information about the migration behavior, with good analytical characteristics and detection limits in the sub mug kg(-1) range. Besides, no significant difference was found between laboratory and factory-made samples, which is an important issue for industrial production, the next step in the development of the new antioxidant active film. 相似文献
42.
Hidalgo A Franzetti L Rossi M Pompei C 《Journal of agricultural and food chemistry》2008,56(4):1289-1297
The aim of this research was to study uracil and lactic and acetic acids as chemical markers for hygienic quality evaluation of raw material in liquid pasteurized egg products. Uracil, absent in sound whole eggs, was formed in raw and pasteurized egg products as a consequence of high microbial contamination (>10(6) cfu/g) after a sufficient lag time, remaining stable at 4 degrees C but disappearing after 7 days of storage at 25 degrees C. Both lactic and acetic acids, starting from initial values of 1-7 mg/kg dry matter, presented trends similar to that of uracil; however, acetic acid never decreased during the storage of raw egg products. With few exceptions, all three metabolites were produced by Enterobacter cloacae, Escherichia coli, Morganella morganii, Serratia liquefaciens, Aeromonas hydrophyla, Pseudomonas fluorescens, Enterococcus avium, and Enterococcus faecalis, separately inoculated in whole egg samples. Uracil seems to be the most sensible marker, with a suggested limit corresponding to the detectable level. 相似文献
43.
44.
45.
Carmela Gallo Giusi Barra Marisa Saponaro Emiliano Manzo Laura Fioretto Marcello Ziaco Genoveffa Nuzzo Giuliana dIppolito Raffaele De Palma Angelo Fontana 《Marine drugs》2020,18(12)
Immunotherapy takes advantage of the immune system to prevent, control, and eliminate neoplastic cells. The research in the field has already led to major breakthroughs to treat cancer. In this work, we describe a platform that integrates in vitro bioassays to test the immune response and direct antitumor effects for the preclinical discovery of anticancer candidates. The platform relies on the use of dendritic cells that are professional antigen-presenting cells (APC) able to activate T cells and trigger a primary adaptive immune response. The experimental procedure is based on two phenotypic assays for the selection of chemical leads by both a panel of nine tumor cell lines and growth factor-dependent immature mouse dendritic cells (D1). The positive hits are then validated by a secondary test on human monocyte-derived dendritic cells (MoDCs). The aim of this approach is the selection of potential immunotherapeutic small molecules from natural extracts or chemical libraries. 相似文献
46.
Monosex male culture of C. quadricarinatus is proposed for increasing yields because males reach a larger size at harvest than females. An experiment was done to evaluate
the effects of androgenic gland extract and temperature on sex reversal and somatic growth in early juvenile females. Females
were exposed to the following experimental conditions: C: control food and temperature maintained at 26 ± 1°C; HT (high temperature):
control food and temperature maintained at 28.5 ± 1°C; VHT (very high temperature): control food and temperature maintained
at 31 ± 1°C; AG: food with enriched with androgenic gland (1/10 dose for each juvenile per day) and temperature maintained
at (26 ± 1)°C. The juveniles were weighed, sexed, and growth increment (GI) and growth rate (GR) were calculated twice a month.
The HT and AG groups differed from the C group in GI and GR indicating a greater somatic growth. At the end of the experiment,
the HT and AG groups had similar weight but only the HT group had enhanced oocyte diameter, with some vitellogenic oocytes
compared to the C group. In the HT group, there was significant sex reversal, as indicated by development of male and intersex
male characteristics in different individuals. Both temperature and AG diet have a high potential for culture of this species
because of their enhancement of somatic growth; higher temperature increases the proportion of males, a desirable characteristic
of cultured populations. 相似文献
47.
Laura Gmez-Montes Zaul García-Esquivel Louis R. D'Abramo Armando Shimada Carlos Vsquez-Pelez María Teresa Viana 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):769-780
Juvenile green abalone Haliotis rufescens were grown under laboratory conditions at 21±1 °C and fed formulated diets consisting of different protein:energy ratios (mg protein/kcal), 62, 74, 85, 100, 108, for 60 days. The level of crude protein ranged from approximately 26% to 44% while the energy content remained constant at about 4.1 kcal g−1. Growth ranged from 3.63 to 12.33 mg day−1. The growth of abalone fed the 100 and 108 diets was significantly greater than that of each of the other diets. Protein efficiency ratio increased as the dietary protein content increased except for the T108 diet (44% crude protein). Abalone apparently consume food to satisfy an energy requirement. Caloric expenditure due to metabolism was estimated for abalone fed diets with protein ratios of 62, 85, 100. Energy loss due to respiration did not vary appreciably among abalone fed the different diets. The proportional distribution of dietary energy into fecal, digestible, growth, and metabolic energy was estimated for abalone fed these diets. Apparent dry matter digestibility was among the lowest for abalone fed the 100 P:E diet, but growth of abalone fed this diet was significantly higher than that of each of the other treatments except the 108 diet. Unexplained energy loss to achieve balance ranged from 7% to 28.5%, some of which is probably due to differential mucus and ammonia production. Results suggest a potential for the reduction of both dietary protein and lipid without causing any adverse effects on the growth response. 相似文献
48.
Larval traits of the Caribbean amphidromous goby Sicydium punctatum (Gobioidei: Sicydiinae) in Guadeloupe
下载免费PDF全文
![点击此处可从《Ecology of Freshwater Fish》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Léa Lejeune Hélène Tabouret Laura Taillebois Dominique Monti Philippe Keith 《Ecology of Freshwater Fish》2016,25(2):272-280
Amphidromous fish are the biggest contributor to the diversity of fish communities in river systems of Caribbean islands. Among them, Sicydium punctatum Perugia, 1896, which is endemic to the West Indies, represents the vast majority of fish in these rivers. The otolith microstructure and the biometry of S. punctatum postlarvae from Guadeloupe were investigated to explore the dispersal modalities of this species through an appreciation of the growth pattern, the pelagic larval duration (PLD) and the size‐at‐recruitment. The study was made on one cohort of 83 recruited postlarvae, fished at the Capesterre River's mouth on 2 November 2011. The mean (±SD) size‐at‐recruitment of the postlarvae was 24.6 ± 1.3 mm (range of 20.5–28.1 mm, n = 83). We found a mean (±SD) PLD of 72.2 ± 10.5 days (range of 54–101.5 days, n = 67). The growth rate estimated from the otolith increments showed a globally decreasing pattern during the marine larval phase. Growth rates at the beginning of the larval stage were significantly different between hatching periods, suggesting a relation between the hatching period and the growth rate in S. punctatum. This study adds on to the general understanding of the life cycle of S. punctatum in Guadeloupe that will help implement strategies to manage amphidromous fish populations in the Caribbean region. 相似文献
49.
George Eng Stephen P. Coddington Laura L. Stockton Alexander D. W. Acholonu 《Pest management science》1989,26(2):117-121
The effect of organotin compounds on the growth of Ceratocystis ulmi, the causative agent of Dutch elm disease, was studied in shake culture. Triphenyltin, (C6H5)3SnX, tricyclohexyltin, (C6H11)3SnX, and tributyltin, (C4H9)3SnX, compounds were all effective in the inhibition of C. ulmi in vitro. The anionic group, X, did not play a major role in the inhibitory activities of these compounds, suggesting that the species involved in the inhibition is R3Sn+ or the hydrated cation. 相似文献
50.
Evaluation of an air-filtration system for preventing aerosol transmission of Porcine reproductive and respiratory syndrome virus
下载免费PDF全文
![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Scott Dee Laura Batista John Deen Carlos Pijoan 《Canadian journal of veterinary research》2005,69(4):293-298
The purpose of this study was to evaluate the ability of a commercial air-filtration system to reduce aerosol transmission of Porcine reproductive and respiratory syndrome virus (PRRSV). The system consisted of a pre-filter and 2 filters with EU8 and EU13 ratings. In each of 4 trials, 5 PRRSV-infected donor pigs and 1 naive recipient pig (each 25 kg) were housed in opposing chambers connected by a 1.3-m-long duct. The system filtered air entering 1 recipient-pig chamber (filtered facility) from the donor-pig chamber but not a 2nd recipient-pig chamber (nonfiltered facility). The donor pigs had been experimentally infected with PRRSV MN-184, an isolate previously documented to be shed at a high frequency in contagious aerosols. On days 3 to 7 after infection of the donors, the 2 groups were housed in their respective chambers for 6 h and then in separate facilities, where samples were collected for testing by polymerase chain reaction and enzyme-linked immunosorbent assay over 14 d. Aerosol transmission was observed in 6 of the 20 replicates in the nonfiltered facility, whereas all pigs remained PRRSV-negative in the filtered facility; the difference was significant at P < 0.01. Thus, under the conditions of this study, the air-filtration system evaluated appeared to be highly effective at reducing aerosol transmission of PRRSV. 相似文献