德菲尔法构建重大动物疫病预警指标体系

[目的 ]构建重大动物疫病暴发流行预警指标体系。[方法 ]以禽流感和口蹄疫为例,采用文献调研法和德菲尔专家咨询法中的专家会议法初拟重大动物疫病预警体系的框架和指标;应用德菲尔专家咨询法评价预警指标的重要性、可操作性;应用组合权重法比较各指标综合影响的大小,并将指标分类。[结果 ]筛选出禽流感和口蹄疫预警预报一级指标6项(易感动物、饲养管理和免疫情况等)以及二级指标14项(免疫效果、环境消毒、温度等),并将指标分为三类(关键指标、重要指标和一般指标)。[结论 ]初步构建了重大动物疫病暴发流行预警指标体系。     

塔落岩黄芪、细枝岩黄芪及其变异型的生理特性研究

本文研究了毛乌素沙漠地区塔落岩黄芪和细枝岩黄芪及其变异型的生理学特性。结果表明,塔落岩黄芪和细枝岩黄芪各与其变异型之间在生理特性方面存在着极其显著的差异。     

杂交狼尾草亲本的开花特性

杂交父本象草Ｎ５１是对光照最为敏感的短日照作物,每日９ｈ,光照处理２～３周,能有效地诱导抽穗开花。象草的穗由分蘖穗和分枝穗组成,由于单株分蘖穗和分枝穗发育程度的差异和群体中个体间的差异致使Ｎ５１群体散粉期可达３０ｄ,散粉高峰期２周。母本Ｔｉｆｔ２３Ａ在南京能完成生育过程,开花期与播期有关,相关系数为０．８８６,群体散粉期与Ｎ５１同样有３０ｄ。由于亲本花期长,因此花期能很好地相遇、杂交、结实     

热敏电阻冰点仪法测定生鲜牛乳中冰点的研究

利用热敏电阻冰点仪法测定生鲜牛乳中的冰点,对该方法的重复性、灵敏度以及掺水掺盐对生鲜牛乳冰点值的影响等方面进行了研究.此方法的检测极限值为2.4 m℃.     

Synergistic effects of Miconazole and Polymyxin B on microbial pathogens

The therapeutic value of antibiotics depends on the susceptibility of the infecting microorganism and the pharmacological profile of the drugs. To assess the value of an antibiotic combination of polymyxin B and miconazole this study examined the in vitro synergistic potential of the two drugs on Gram-negative and Gram-positive bacteria and yeast. Antifungal and antibacterial activity was tested by minimum inhibitory concentration (MIC) of broth macrodilution and urea broth microdilution, by fluorescence microscopy and flow cytometry. Synergism was calculated using the fractional inhibitory concentration index (FICi). With Staphylococcus intermedius as target we found up to an eightfold reduction of the individual MICs when both drugs were combined. However, the FICi was 0.63 suggesting no real interaction between the two drugs. With Escherichia coli, Pseudomonas aeruginosa, and Malassezia pachydermatis as targets the antimicrobial drug combination reduced the MICs of polymyxin B and miconazole from fourfold to hundredfold resulting in FICi between 0.06 and 0.5 which defines a synergistic action. Thus, if polymyxin B and miconazole are combined their effect is greater than the sum of the effects observed with polymyxin B and miconazole independently, revealing bactericidal and fungicidal synergism. Our results indicate a strong therapeutic value for the combination of these antimicrobial agents against Gram-negative bacteria and yeast and a weaker value against Gram positive bacteria for clinical situations where these pathogens are involved.     

<Emphasis Type="Italic">Streptococcus uberis</Emphasis>-specific T cells are present in mammary gland secretions of cows and can be activated to kill <Emphasis Type="Italic">S. uberis</Emphasis>

The presence, phenotype and function of Streptococcus uberis-specific T cells in the mammary gland secretion (MGS) and blood of cows exposed to S. uberis were assessed. MGS T cells in the udder were purified and incubated with autologous blood monocytes as antigen-presenting cells (APC). Most cows, irrespective of prior S. uberis infection status and lactation status, were shown to have S. uberis-specific T cells both in MGS and in the blood. When cells from a subgroup of cows were studied, it was found that the S. uberis-specific T cells produced high levels of interferon-gamma (IFN-γ), but low levels of interleukin-10 (IL-10). A high percentage of responding T cells were of the CD8 ⁺ memory (CD45RO) subset. T cells from the MGS specific for S. uberis were propagated from animals during the drying off period and expanded in vitro using interleukin-2 (IL-2) and S. uberis antigens. This led to the accumulation of T cells of the CD8 ⁺ subset bearing memory cell markers (CD45A ⁻ , CD45RO ⁺ ), which released high levels of IFN-γ. Four of the five T cell lines derived from the MGS of three animals had substantial direct killing activity towards S. uberis in vitro. It is concluded that there is an emergence of S. uberis-specific bactericidal T cells in the MGS of cows after infection or environmental exposure to S. uberis. Vaccines aimed at activating and expanding this T cell population in the mammary glands of cattle may offer an avenue for the prevention of mastitis caused by S. uberis.     

不同激素剂量对幼鼠超数排卵及超排卵质量的影响

应用国产激素制剂对３～８周龄昆明种小白鼠进行了超数排卵处理,重点观察了不同激素剂量对幼龄小鼠的超排效果及超排卵质量的影响。实验结果表明,以ＰＭＳＧ与ｈＣＧ各１０ＩＵ剂量超排３～８周龄小鼠,５周龄小鼠的超排效果（平均获卵８３．８±１０．２个）极显著（Ｐ＜０．０１）好于４～８周龄小鼠的超排效果（平均获卵３９．２～４９．８个）,而３周龄小鼠超排卵受精后体外培养的卵裂率（９７．１％）、桑椹胚率（８６．８％）和囊胚率（６５．１％）与４～８周龄超排卵的（分别为８５．５％～１００．０％、９４．９％～１００．０％和６３．２％～８７．７％）相近;以ＰＭＳＧ与ｈＣＧ各５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵５９．０±６．５个）显著（Ｐ＜０．０５）好于４周龄小鼠的超排效果（平均获卵３１．４±４．９个）,并极显著（Ｐ＜０．０１）好于５周龄小鼠的超排效果（平均获卵２１．４±３．３个）;以ＰＭＳＧ与ｈＣＧ各１５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵４８．６±７．３个）显著（Ｐ＜０．０５）好于５周龄小鼠的超排效果（平均获卵２５．２±５．８个）,也好于４周龄小鼠的超排效果（平均获卵４０．２±５．１个     

我要尽情地去爱

在很多不喜欢动物的人眼里,狗是一种低等动物,可他们根本不知道狗是怎样对待生活的。     

饲料级硫酸铜中砷的原子吸收--氢化物法测定

建立了一种快速、灵敏、准确测定硫酸铜中砷含量的新方法。本法加入硫氢酸铵和盐酸羟铵使铜沉淀,过滤分离,再用原子吸收—氢化物法测定砷,避免了大量的铜干扰,使测定结果准确可靠。线形范围2～20ng/ml,相关系数R=0.9990,RSD1.64%,平均回收率96.0%～102%。     

刑侦犬锁定"苦行僧"毒贩--云南腾冲"3·12"特大跨国贩毒案侦破纪实

这是一起在云南警方组织开展的“天网三号”禁毒专项行动中多警种协作、群众踊跃参与、警犬投入侦破的典型案例:2006年3月12日,当3名嫌疑人面对从两公里外查获的3背包沉甸甸的毒品海洛因及其他赃物拒不认罪、案件侦查陷入僵局时,刑侦犬“安普贵”奉命出击,把毒品和毒贩锁定在一