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In some pathophysiological processes such as severe shock, the patients and animal experimental models fail to respond to pressor medicine. Vascular hyporesponse or vasodilation is one of the key problems in treatment of these patents. Although considerable progress had been made in recent years, the precise mechanisms of vascular contractile hyporeactivity have not been completely elucidated. A number of studies indicate that vascular hyporesponse or vasodilation correlate with many factors. In this review, the possible mechanisms about vascular hyporesponse or vasodilation during shock and other pathphysiolgoical processes were discussed. 相似文献
33.
AIM:To investigate the effects of FK506 on anti-glomerular basement membrane(GBM) nephritis in rats. METHODS: Anti-GBM nephritis model was elaborated by rabbit anti-rat GBM antibody injection in SD rats in this study. The rats were divided into three groups: FK506 treated group(0.5 mg·kg-1·d-1, sc), untreated nephritis control group and normal control group. FK506 was administered daily six hours after injection of anti-GBM IgG. All the rats were observed urinary protein at the 4th day, the 14th day and the 21st day. At the same time, the kidney specimens were collected, and T cell transforming function was also monitored.RESULTS:Rats injected with rabbit anti-GBM Ab developed heavy proteinuria by 4 days, and serum creatinine and serum urea appeared which kept on the rising. Glmerular hypercellularity, crescents, and protein casts were observed in nephritic rats. By electron microscopy, the thickening of GBM and loss of foot processes were seen. T cell transforming function was higher than normal. But, all pathological changes obviously turned for the better in FK506 treated group. CONCLUSION:FK506 could inhibit the progression of rat anti-GBM nephritis. 相似文献
34.
菟丝子致死薇甘菊 总被引:10,自引:0,他引:10
薇甘菊Mikania micrantha是入侵的害草,已经由珠江三角洲扩散到粤东、粤西沿海地区,以及部分山区。在薇甘菊的天敌调查中看到菟丝子寄生于薇甘菊,严重时可以把薇甘菊致死。我们在本所的实验园中栽种薇甘菊,并让其被菟丝子Cusuta chinensis Lam.寄生,结果证实菟丝子在两个月左右,完全可以抑制薇甘菊的生长,最终把薇甘菊致死。这可能是以草治草的一种新途径,但是菟丝子也是我国农作物的一种重要昌,如何利用菟丝子控制薇甘菊的为害,而又不使菟丝子对薇甘菊的伴生植物和鞭它农作物造成为害,是下一步要解决的问题,菟丝子致死薇甘菊的机理更需要深入研究。 相似文献
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AIM: To prepare gfp-bcl-XL-contained recombinant adenovirus(rAd-gfp-bcl-XL).METHODS: Bcl-XL gene was amplified from pEGFP-C3-bcl-XL, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-XL. Then pAdTrack-CMV-bcl-XL was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-XL and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-XL into 293 cells. PCR test indicated that the recombinant Ad contained bcl-XL gene. The titer of the purified rAd-gfp-bcl-XL was 6.5×1012 PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-XL. This affords a good gene transfer vector for the gene therapy in human’s diseases. 相似文献
38.
AIM:To investigate the mechanism responsible for albumin microbubbles adherence to activated leukocytes. METHODS: In vitro studies were performed in which activated or nonactivated leukocytes were incubated with albumin microbubbles and observed under microscopy. The suspensions of leukocytes and microbubbles which contained or absented of integrins were analyzed with flow cytometry.RESULTS: A minimum of 50cells were identified under transillumination. 5 min after microbubbles were incubated with leukocytes, the number of cells interacting with microbubbles was greater for activated cells than for nonactivated cells(20.30±2.67 vs 4.50±1.43, P <0.01).Microbubbles attached to the surface of activated leukocytes were phagocytosed and remained intact for up to 30min. Microbubble attachment was inhibited notably by blocking the leukocyte β2-integrin Mac-1(P <0.01) and by VLA-4mAb slightly(P <0.05) CONCLUSION: The mechanism of albumin microbubbles attaching to and phagocytosed by leukocytes was due to β2-integrin and VLA-4 mediation. Phagocytosed microbubbles can remain at the regions of inflammation for15 min, also responsible to ultrasound. 相似文献
39.
AIM:To investigate the changes in intracellular potassium activity(aiK) and membrane potential(Vm) induced by low external sodium infusion (Low [Na]o) and to detect the mechanisms involved and the relationship between aiK and Vm. METHODS:aiK and Vm were measured in infusion with different sodium concentrations using methods of convenient and ion selective microelectrodes in guinea pig ventricular myocardium. RESULTS:Low [Na]o resulted in a decrease in aiK and an increase in Vm in a Na+ concentration-dependent manner.At the same time,contraction and resting potential increased, and action potential duration decreased markedly,but action potential amplitude was not affected. A change of the pH from 7.4 to 7.0 in low [Na]o solution reduced the decrease in aiK, but did not affect the increase in Vm.CONCLUSION:A better linear relationship appeared between the changes in aik and [Na]o or in Vm and [Na]o,while during each low [Na]o the change in both aiK or Vm may reach a new balance level. 相似文献
40.