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91.
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双孢蘑菇性亲和性相关分子标记的初步筛选 总被引:4,自引:0,他引:4
以传统的形态,生理生化分析和最新的DCS-PDMA性亲和性测定方法为基础, 结合群体分离分析和RAPD技术来源于同一双孢蘑菇异核体菌株的12个不育同核原生质体个体进行分析,筛选与性亲和性相关的分子标记。研究结果表明,供试的12个不育同核原生质体个体被分成两大类性亲和性类型,其中一类(A^ )包括不育同核原生质体个体B、C、D、E、F、G、H、I、J、L,另一类(A^-)则仅仅包括不育同核原生质体个体K和M,同时筛选到一个与性亲和性相关的分子标记OPA16 1500。从而为间地利用双孢蘑菇本身特有的交配型作标记来指导杂交育种工作和进一步将性亲和性基因定位分离克隆奠定了坚实的基础。 相似文献
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苹果粗皮病与锰含量的关系 总被引:14,自引:2,他引:14
对胶东半岛部分发生苹果粗皮病(IBN)的果园的土壤和植株进行了分析测定,结果表明:发生苹果粗皮病的果园土壤有效锰含量较高,而且粗皮病严重果园高于粗皮病较轻果园。发生粗皮病的苹果树花器官中锰含量较高,粗皮病最严重的姜格庄果园苹果树花器官中锰平均含量比观水镇高出139.28mg/kg,比季家埠重病园和轻病园分别高122.34和182.19mg/kg。从4月到10月,苹果粗皮病逐渐加重,叶片中锰含量逐步增高。10月份姜格庄果园最高锰含量达1717.85mg/kg,平均锰含量达1119.69mg/kg,而粗皮病较轻的季家埠果园则仅306.03mg/kg,前者比后者高265.88%。对不同部位锰含量测定表明,锰含量在叶片中最高,其次是韧皮部,木质部最低。表明胶东半岛苹果粗皮病的发生是因为锰过量。可能是土壤锰含量高,pH值低,降雨多,施肥不合理等,造成土壤锰有效性增加.导致树体吸收过量而出现粗皮病害。 相似文献
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AIM:To establish the monoclonal antibody against human B lymphocyte stimulator (hBLyS) by DNA immunization and analyse its characterization. METHODS:The 858 bp DNA fragment of hBLyS was cloned into pcDNA3 plasmids. The cloned insert was identified by both sequence analysis and double digestion of the recombinant plasmid with restriction enzymesXho Iand EcoR I. After the splenocytes from BALB/c mice immunized with the recombinant plasmid of pcDNA3/hBLyS were fused with myeloma cells SP2/0,the hybridoma which can produce monoclonal antibodies against hBLyS were obtained. The specificity of anti-BLyS monoclonal antibody from hybridoma was verified by ELISA, Western blot and flow cytometry. RESULTS:The recombinant mammalian cell expression vector of pcDNA3/hBLyS was constructed,the sequence of the insert gene was identified to be the sequence encoding hBLy S antigen. The culture supernatants of hybridoma 9c10 were tested to be the monoclonal antibody with specificity against hBLyS on human peripheral blood CD3+T cell activated by hIFN-γ by ELISA,Western blot and flow cytometry.CONCLUSION:The monoclonal antibodies against hBLyS with high activity and specificity have been established successfully, and will be an useful tool in the studies of relationship between hBLyS and human autoimmunity diseases. 相似文献
98.
LIN Jun SUN Jie ZHOU Yan HUANG Xing XIONG Ping WANG Ya-ping DENG Chang-sheng 《园艺学报》2002,18(9):1112-114
AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls(0.2500 vs 0.1397, P =0.028; the odds ratiO2.053; etiologic fraction 0.1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exoN2)published in GenBank. 相似文献
99.
AIM:To investigate the mechanism responsible for albumin microbubbles adherence to activated leukocytes. METHODS: In vitro studies were performed in which activated or nonactivated leukocytes were incubated with albumin microbubbles and observed under microscopy. The suspensions of leukocytes and microbubbles which contained or absented of integrins were analyzed with flow cytometry.RESULTS: A minimum of 50cells were identified under transillumination. 5 min after microbubbles were incubated with leukocytes, the number of cells interacting with microbubbles was greater for activated cells than for nonactivated cells(20.30±2.67 vs 4.50±1.43, P <0.01).Microbubbles attached to the surface of activated leukocytes were phagocytosed and remained intact for up to 30min. Microbubble attachment was inhibited notably by blocking the leukocyte β2-integrin Mac-1(P <0.01) and by VLA-4mAb slightly(P <0.05) CONCLUSION: The mechanism of albumin microbubbles attaching to and phagocytosed by leukocytes was due to β2-integrin and VLA-4 mediation. Phagocytosed microbubbles can remain at the regions of inflammation for15 min, also responsible to ultrasound. 相似文献
100.