气相色谱-串联质谱法测定柑橘中5种杀螨剂残留

建立了气相色谱-串联质谱法(GC-MS/MS)检测柑橘中5种杀螨剂残留分析方法。样品经乙腈提取、氯化钠盐析后,取有机相用Qu EChERS方法净化,利用GC-MS/MS在多反应监测(MRM)模式下进行测定,外标法定量。5种杀螨剂在0.005~0.5mg/L浓度范围内呈现良好的线性关系,相关系数r^2均>0.996,方法定量限为0.005 mg/kg。在0.005、0.01和0.1mg/kg 3种添加水平下,加标回收率为70.6%~111.3%,相对标准偏差(RSD)为1.07%~14.64%。本方法简便、重现性良好,可用于柑橘类水果样品中杀螨剂的日常检测。     

叶面喷施外源物质对黄瓜商品苗贮运质量的影响

为了探究叶面喷施外源物质对黄瓜商品苗贮运质量的提升效果,研究蔬菜育苗生产中常用的寡聚糖、壮苗1号和植物益生源对贮运0、3、6 d黄瓜穴盘苗质量的影响。结果表明:与喷施清水的对照相比,壮苗1号可以抑制幼苗徒长,增加地下部干质量,维持较高的壮苗指数、比叶重和叶绿素含量,而植物益生源和寡聚糖对幼苗各生长指标的影响较小。因此,推荐叶面喷施壮苗1号来提高黄瓜商品苗的贮运质量。     

山东地区无公害牛蒡标准化生产技术规程

为发挥山东省牛蒡种植面积大、栽培历史久的天然优势,巩固其全省重要出口创汇蔬菜的独特地位,对牛蒡的产地环境、种子处理、播种时间和方法、田间肥水管理、排灌沟渠安排、病虫草害防治、采收分级、生产记录等制定出规范与要求,标准化生产的牛蒡每667 m2产量在1 500 kg以上,收购价格保持在2.6~3.8元/kg,为指导蔬菜生产,确保农产品质量安全,增加产品出口和农民收入提供了技术和制度上的保障。     

外源褪黑素对干旱胁迫下紫苏种子萌发的影响

以紫苏(Perilla frutescens(L.)Britton)为试材,利用聚乙二醇(PEG-6000)模拟干旱胁迫环境,分析不同浓度外源褪黑素对紫苏种子萌发特征(发芽率、发芽势、发芽指数和活力指数)和幼苗生长(根长、子叶长、子叶宽和鲜质量)的影响。结果表明:干旱胁迫下紫苏种子萌发受到显著抑制,经过不同浓度的外源褪黑素处理后,发芽指标明显上升,其中处理T1(10%聚乙二醇+50μmol·L-1褪黑素)各项指标均达到最大值,发芽率为64.00%,发芽势为43.67%,发芽指数为17.62,活力指数为0.065。外施褪黑素同样可以缓解干旱胁迫下紫苏幼苗的生长,其中处理T3(10%聚乙二醇+300μmol·L-1褪黑素)效果最好,幼苗根长为7.45mm,子叶鲜质量为3.53 mg,长宽比为1.260。表明外施一定浓度的褪黑素能有效缓解干旱胁迫对紫苏种子萌发的影响,为干旱环境下紫苏种植栽培提供了参考依据。     

猪瘟病毒衣壳蛋白与宿主丝/苏氨酸蛋白激酶N1相互作用的鉴定

为筛选并鉴定与猪瘟病毒(CSFV)衣壳(C)蛋白相互作用的宿主蛋白,本研究采用酵母双杂交技术以CSFV C蛋白为诱饵从猪外周血单个核细胞(PBMC)c DNA表达文库中筛选与之相互作用的宿主蛋白,共筛选到6种蛋白,分别为ATP5B、SON3、PKN1、PCBP1、RPS20和IQGAP1,根据Gene Ontology分析结果,这些蛋白分别参与细胞的增殖和代谢等过程。本研究选取丝/苏氨酸蛋白激酶N1(PKN1)进行进一步验证,经酵母共转化试验、免疫共沉淀试验和GST pull-down试验证实,宿主PKN1蛋白与CSFV C蛋白之间存在特异性结合。本研究首次证明PKN1与CSFV蛋白之间的相互作用关系,为进一步研究PKN1蛋白在CSFV感染过程中发挥的作用奠定了基础。     

基于称重式蒸渗仪的温室菜心耗水规律

以菜心为试材,利用称重式蒸渗仪,以直径20cm的蒸发皿水面蒸发量(Ep)为基础标准,研究了在不同灌溉处理条件下(0.6E_p、0.7E_p、0.8E_p、0.9E_p)温室菜心生育期的蒸散量、蒸散速率、产量、生物量及水分利用效率,以探明温室菜心的耗水规律,明确温室菜心合理的灌溉量。结果表明:温室菜心生育期内蒸散量波动范围为0.73~3.52mm·d-1,前期及中后期的平均蒸散速率分别为1.43mm·d~(-1)和2.04mm·d~(-1),呈前期变化平稳,中后期变化剧烈的趋势;随着灌溉量的增加,菜心的蒸散量随之增加,温室菜心全生育期蒸散量为65.98~78.92mm。其中0.7E_p处理下温室菜心产量、生物量、产量水分利用效率及生物量水分利用效率均为最高。与0.6E_p、0.E_p处理和0.9E_p处理相比,0.7Ep处理的产量分别提高了25.16%(P0.05)、5.63%和13.76%(P0.05),产量水分利用效率分别提高了17.42%(P0.05)、8.48%和27.66%(P0.05),生物量分别提高了21.96%(P0.05)、7.41%和17.68%(P0.05),生物量水分利用效率分别提高了11.21%、10.32%和32.10%(P0.05),0.7E_p灌溉量为供试条件下温室菜心的最优灌溉量。     

抚顺地区一次温度预报错误分析

针对2012年3月7日抚顺地区一次温度预报出现明显偏差的情况,从高低空环流形势和地面形势等方面对气象过程进行了概述,并从温度平流、垂直运动和非绝热因子方面对预报失误的原因进行了分析。     

Identification and Molecular Characteristics Analysis of Porcine Epidemic Diarrhea Virus Variants

To ascertain a diarrhea case in a pig farm in Shandong province,the pathological changes of dead piglets were observed and nested RT-PCR test was carried out on 7 diarrhea samples for porcine epidemic diarrhea virus (PEDV).Pathological examination revealed that intestine was detected as enlargement,hyperemia and edema.After histological examination,the typical microscopic lesions of intestine were disappearance of epithelial cells,villus shrinkage and shortening.The result of nested RT-PCR showed that all of the 7 samples could amplify a specific target band of PEDV.Molecular characteristics of two field strains showed that they had an amino acid homology of 92.3% to 92.4% with vaccine CV777 and 96.6% to 98.6% with other previous field strains which sequences were downloaded from GenBank.Phylogenetic tree analysis further revealed that all of PEDV strains could be mainly divided into two clusters of G1 and G2. G2 consisted of the field strains of our study and other field strains from USA,China and so on,which had the same sequence characteristics of two insertions and one deletion,while G1 consisted of all vaccines of CV777 and several older field strains from China and Korea.These results indicated that our field strains were the dominant strains in recent epidemic diarrhea occurrence.Moreover,its molecular characteristics might be a characterization of differentiating the field and vaccine strains.     

Pathogen Analysis of Goat's Respiratory Diseases

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.     

大豆种子寄藏真菌致病性测定方法比较

寄藏真菌可侵染大豆种子,降低种子的萌芽及活力,影响种子的品质和商品等级。为探讨不同接种方法对寄藏真菌致病性的影响,在实验室条件下比较了种子接种法和植株接种法(切顶端接种法、伤口接种法、切茎接种法和下胚轴接种法)对寄藏真菌致病性的影响。结果表明:各方法中寄藏真菌均对大豆产生致病性,并能显著区分寄藏真菌间致病性差异。其中切顶端接种法相比其他3种植物接种方法更简单有效而准确,而种子接种法更适合在室内进行,且周期短,相比植株接种法方便快捷。