Improvement in growth and alkaloid content of Rauwolfia serpentina on application of organic matrix entrapped biofertilizers (Azotobacter chroococcum,Azospirillum brasilense and Pseudomonas putida)

The field experiment was conducted to study the effect of conventional chemical fertilizer (urea),conventional biofertilizers (charcoal based) and organic matrix entrapped biofertilizer; (OMEB: a consortium of Azotobacter chroococcum, Azospirillum brasilense and Pseudomnas putida entrapped with clay soil, neem leaves, and cow dung in ratio of 1:1:1 and 15% saresh (plant gum of Acacia sp.) in various doses on growth parameters of Rauwolfia serpentina in terms of root length, shoot length, number of leaves, number of roots and fresh and dry weight of roots and shoots. Replacement of conventional chemical fertilizer by the consortium of biofertilizers (un-entrapped) increased plant growth in a dose dependent manner up to triple dose of the recommended dose (RD) of biofertilizers (1.80 kg ha-1). This increase in plant growth was not up to the level which appeared in the application of RD of urea. Entrapment of the same biofertilizers in an organic matrix mentioned above enhanced the plant growth similar to that observed for urea. The percentage increase of 9.57 and 7.54 in fresh weight (FW) and 11.93 and 11.12% in dry weight (DW) of shoot and 17.94 and 37.87% in FW of roots and 31.90 and 50.08% in DW of roots were recorded in 75 days old plant by the application of OMEB- triple dose over the conventional biofertilizers triple dose and recommended dose of urea. Conventional biofertilizers enhanced the availability of nitrate, nitrite and phosphate in the roots and leaves of the plant over application of urea as well as conventional biofertilizers. This formulation also increased alkaloid contents of in the roots of this plant. Microbial population of soil was also improved indicating enhanced soil fertility with application of OMEB. The results indicate that replacement of chemical fertilizers with microbial biofertilizers is possible with application of higher dose of biofertilizers entrapped into the organic matrix for the cultivation of medicinal plants like R. serpentina.     

Greenhouse gases emission,soil organic carbon and wheat yield as affected by tillage systems and nitrogen management practices

Agricultural activities are responsible for greenhouse gases (GHGs) emission in the environment. Strategies are required to enhance the soil organic carbon (SOC) and nitrogen (N) sequestration to adapt and mitigate the climate change. We investigated GHGs emission, SOC and N enhancement under conventional tillage (CT) and zero tillage (ZT) with N management in wheat (Triticum aestivum L.). Seasonal carbon dioxide (CO₂) emission and global warming potential (GWP) reduced for ZT treatments over CT without residues and 100% of required N with a blanket split application (CT – R + 100N). The ZT with 5 t ha^?1 maize (Zea mays L.) residues retention and 75% of required N and GreenSeeker^TM (GS)-aided N management (ZT + R + 75N + GS) reduced yield-scaled GHGs emission and increased total organic carbon (C) stock over CT – R + 100N. However, nitrous oxide (N₂O) emission was lower in CT. The GS-based N management saved 26–35 kg N ha^?1 in different tillage systems in both years over blanket application with higher N uptake and associated reduction in N₂O emission. The study recommends that ZT with residues retention and GS-based N management can minimize the GHGs emission and improve the SOC.     

Effects of pre- and post-inoculation photoperiod on the severity of Melampsora medusae leaf rust of Populus species

Disease severity, based on six parameters (time from inoculation to flecking, and eruption of uredinia, uredinial density and the amounts of urediniospoies produced), was assessed on leaf discs cut from four cultivars of Populus spp. raised with either a long (15 h) or a short (10 h) photoperiod. The discs were inoculated with race 4 of Melampsora medusae and subsequently incubated with either a long (15 h) or a short (10 h) photoperiod. While disease severity, based on most parameters, was lower in discs from the continuing long photoperiod (15 h pre- and 15 h post-inoculation) than from the continuing short photoperiod (10 h pre- and 10 h post-inoculation), maximum severity was developed by discs given a combination of a short pre- and a long post-inoculation photoperiod. The relative contribution of the cultivar, the pre- and the post-inoculation photoperiod and their second- and third-order interactions to variation in disease severity depended on the parameter employed to assess severity. The parameters used are elements of disease monocycles and their possible significance in modelling epidemics is discussed.     

In vitro antibiotic resistance profiles of Ornithobacterium rhinotracheale strains from Minnesota turkeys during 1996-2002

Antimicrobial resistance in nearly all human and animal pathogens is on the increase. In poultry, Ornithobacterium rhinotracheale has been identified as a newly emerging respiratory bacterial pathogen that has caused significant economic losses to the poultry industry. In this study, we examined in vitro antibiotic resistance profiles of 125 isolates of O. rhinotracheale isolated from turkeys in Minnesota during 1996-2002. A majority of isolates was sensitive to clindamycin, erythromycin, spectinomycin, and ampicillin. Resistance against sulfachloropyridiazine decreased from 1996 to 2002, but an increase in resistance was seen against gentamicin, ampicillin, trimethoprim sulfa, and tetracycline. The annual trend slopes for these antibiotics were 7.36%, 3.02%, 2.43%, and 1.95%, respectively. The resistance against penicillin remained constant from year to year with a trend slope of only 0.54% per year. These results emphasize the need for continued monitoring of O. rhinotracheale isolates for antibiotic resistance and establishment of baseline resistance pattern data for this organism. These data can then be used to design and evaluate local epidemiological interventions.     

Biological, serological and molecular characterization of a cucumber mosaic virus isolate from India

A. virus causing mosaic and leaf deformation of Physalis minima has been identified as an isolate of cucumber mosaic virus (CMV) on the basis of its transmission by aphids in a non-persistent manner, polyhedral particles of 29 nm diameter, molecular weight of coat protein subunits us 24-5 kDa. serological relationship with a CMV isolate and a tripartite single-stranded RNA genome with a subgenomic RNA4- Furthermore. cDNA representing coat protein gene was synthesized and cloned. Complete nucleotide sequences (890 nt) were obtained which showed a coat protein gene open reading frame of 657 residues. THE nucleotide sequences provided the 218 amino ACID sequences of the coat protein. Nucleotide as well as amino acid sequences revealed more than 90% identity with the CMV subgroup I strains.     

RADIOGRAPHIC DIAGNOSIS OF BOVINE HORN CANCER (SQUAMOUS CELL CARCINOMA)

Characteristic radiologic differences were observed between the normal and cancerous horns of five bullocks. In the latter, the horn core was replaced by a homogenous soft tissue mass without demarcating osseous landmarks. The horn appeared osteolytic in nature. It was evident that lysis of core started from the tip and progressed toward the base. Prognosis was favorable when the base was not involved.     

Occurrence of a strain of eggplant mottled crinkle virus in Solanum capsicastrum in India

Leaf crinkle symptoms, stem necrosis and stunting were observed in Solanum capsicastrum plants in India. A virus was isolated from these plants and identified as a strain of eggplant mottled crinkle virus based on host reactions, in vitro properties, icosahedral particle morphology, buoyant density (1.39 g cm³), molecular weight (41 ± 0.5 kDa) of coat protein subunits and serological relationships     

Spontaneous subcutaneous myxosarcoma in a captive European hedgehog (Erinsceus europaeus).

A surgically excised biopsy representing a subcutaneous mass on the left side of the neck from a 3-year-old female European hedgehog (Erinsceus europaeus) was presented. Spontaneous myxosarcoma was diagnosed based on histological, immunohistochemical, and ultrastructural characteristics. The neoplasm grossly consisted of a firm, pale, multilobulated mass with a characteristic clear gelatinous fluid. Histologically, the neoplasm was nonencapsulated and composed of pleomorphic stellate or spindle-shaped vimentin and periodic acid-Schiff-positive cells arranged in loose sheets and occasionally whorls. The neoplastic cells were suspended in Alcian blue-positive stroma and contained infrequent mitotic figures. Evidence of a viral etiology was not detected using electron microscopy and polymerase chain reaction. This is the first case report of a myxosarcoma in a captive European hedgehog.     

Importance of brucellosis control programs of livestock on the improvement of one health

Brucellosis not only represents an important health restraint on livestock but also causes high economic losses in many developing countries worldwide. Despite considerable efforts made for the control of brucellosis, the disease is still spreading in many regions (such as the Middle East) where it represents one of the most important health hazards impacting both animals and humans. The present review aims to investigate the efficacy of veterinary control programs regarding brucellosis, with a special focus on current prevention, control, and eradication approaches. The reasons for unsuccessful control programs such as the absence of highly effective vaccines and non-certified bulls are also debated, to understand why the prevalence of brucellosis in livestock is not decreasing in many areas despite considerable efforts taken to date. The importance of governmental and regional investment in brucellosis control remains one of the main limiting factors owing to the limited budget allocated to tackle this disease. In this context, one health concept has generated novel comprehensive approaches with multiple economic implications across the livestock industry and public health. However, the implementation of such global preventive strategies appears to be a key issue for many endemic and low-income countries. According to the collected data, epidemiological contexts including management and trade systems along with well-defined agro-ecological zones should be evaluated in brucellosis endemic countries to improve milk production and to enhance the sustainability of the livestock sector at both national and regional levels.     

Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review

Mastitis (intramammary inflammation) caused by infectious pathogens is still considered a devastating condition of dairy animals affecting animal welfare as well as economically incurring huge losses to the dairy industry by means of decreased production performance and increased culling rates. Bovine mastitis is the inflammation of the mammary glands/udder of bovines, caused by bacterial pathogens, in most cases. Routine diagnosis is based on clinical and subclinical forms of the disease. This underlines the significance of early and rapid identification/detection of etiological agents at the farm level, for which several diagnostic techniques have been developed. Therapeutic regimens such as antibiotics, immunotherapy, bacteriocins, bacteriophages, antimicrobial peptides, probiotics, stem cell therapy, native secretory factors, nutritional, dry cow and lactation therapy, genetic selection, herbs, and nanoparticle technology-based therapy have been evaluated for their efficacy in the treatment of mastitis. Even though several strategies have been developed over the years for the purpose of managing both clinical and subclinical forms of mastitis, all of them lacked the efficacy to eliminate the associated etiological agent when used as a monotherapy. Further, research has to be directed towards the development of new therapeutic agents/techniques that can both replace conventional techniques and also solve the problem of emerging antibiotic resistance. The objective of the present review is to describe the etiological agents, pathogenesis, and diagnosis in brief along with an extensive discussion on the advances in the treatment and management of mastitis, which would help safeguard the health of dairy animals.