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101.
ABSTRACT: The thermal stability of carp G-actin was investigated by monitoring loss of actin polymerization ability. To determine the amount of native actin remaining after heat treatment, actin was labeled with a fluorescence reagent, N-(1-pyrene)iodoacetamide. The loss of polymerization ability of carp actin during heat treatment, at between 45 and 55°C, occurred faster than that of chicken actin. The inactivation rate was influenced by concentrations of ATP and Ca2+ in solution. With the increase of Ca2+ concentration, the inactivation of carp actin was markedly suppressed. Furthermore, the activation energy of the inactivation of carp actin obtained from an Arrhenius plot was similar to that of chicken actin. These results indicated that the thermal instability of carp G-actin was due to the low affinites of ATP and Ca2+ for carp actin described in a previous report. 相似文献
102.
The present study examines the pattern of accumulation of cadmium (Cd) and its biochemical effects on selected tissues of
a variety of African catfish (Clarias gariepinus), after exposure to various doses of Cd. The results obtained indicate that at the end of 21 days of exposure, the total
tissue organ cadmium concentration followed the pattern kidney > gill > liver > muscle for each of the exposure concentrations.
The levels of Cd in these organs were higher than those in ambient water. Moreover, while the rate of uptake of Cd increased
with time in the kidney, liver and muscle, it decreased in the gill. Superoxide dismutase (SOD) was significantly elevated
only in the kidney of catfish treated with 0.2 and 0.4 ppm of Cd for 7 days compared with the control. Conversely, gill SOD
was significantly decreased in the same concentrations of Cd-treated catfish relative to the control. Statistically similar
levels of SOD were observed in the liver, brain and muscle with all the treatments after the same duration of treatment. In
the fish exposed for 21 days, SOD activity was significantly decreased in the kidney with a corresponding increase in lipid
peroxidation (LPO), but it manifested only with the 0.2- and 0.4-ppm Cd treatment relative to the control. In the liver, however,
Cd exposure significantly increased SOD in the 0.2- and 0.4-ppm treatments of the same duration. In conclusion, the present
study indicates that the accumulation of Cd and its effect on SOD and LPO in C. gariepinus is dependent on concentration, tissue and time 相似文献
103.
Differential HSP90α expression in fish hepatocytes from polluted estuary during summer 总被引:2,自引:0,他引:2
The molecular chaperone, heat shock protein 90α (HSP90α), plays an important role in protein folding, degradation of denatured
proteins and steroid activation. It is essential for the maintenance of cellular integrity and survival when induced in response
to environmental, physical and chemical stresses. In the present investigation the effect of environmental stress on HSP90α
expression was examined in grey mullet Mugil cephalus living in either a contaminated (Ennore) or uncontaminated (Kovalam) estuary over two seasons: Hepatocytes were isolated
from grey mullet of both estuaries. Oxidative stress was determined along with HSP90α in these fish. Additionally, immunohistochemical
changes were studied to confirm the HSP90α expression. Comparison of the results revealed enhanced hepatocyte oxidative stress
and HSP90α expressions in fish from Ennore to a significant extent than fish from Kovalam. Also, the results showed significant
seasonal variations with maximum expression observed during summer compared to the monsoon season. Overexpression of HSP90α
in hepatocytes exposed to chronic environmental stress by pollutants may confer differential effects on cell survival by protecting
against oxidative stress induced changes. The results also indicate that seasonal variations have significant effect on the
HSP90α expression. 相似文献
104.
Specific dynamic action (SDA), the energy costs associated with meal digestion and assimilation, is generally affected by
body size and food composition. We assessed the postprandial metabolic response and calculated SDA in two size groups of the
southern catfish (Silurus meridionalis), each fed one of two diets, high lipid or high carbohydrate, at a meal size of 4% the body mass. Using a continuous-flow
respirometer, we determined the oxygen consumption rate at 2-h intervals until the postprandial oxygen consumption rate returned
to the prefeeding level. None of the parameters (resting metabolic rate, Rpeak, factorial ratio, time-to-peak, duration, energy
expended on SDA, or SDA coefficient) were significantly affected by diet nor was there an interaction between diet and body
mass. Rpeak and energy expended on SDA for the whole fish body were significantly higher in the larger fish than the smaller
one in both dietary treatments, whereas no significant effect of body size was found in mass specific values. Factorial ratio
(range 3.41 to 3.60), peak time (range 9.6 to 12.7 h), SDA coefficient (range 9.36 to 10.36%), and SDA duration (range 62.0
to 71.0 h) did not significantly differ between body size groups. These results suggest that in S. meridionalis the percentage of assimilated energy allocated to SDA may be independent of the body mass. 相似文献
105.
Tomoki Hashimoto Katsuya Hyodoh Takuma Hirose Satoshi Nishikawa Toshiya Katano Shin-ichi Nakano 《Aquaculture International》2008,16(4):309-318
In the pearl cultivation farms of the Ehime Prefecture, Japan, mass mortalities of the pearl oyster Pinctada fucata have occurred since 1994. The occurrences of mass mortality roughly coincided with a shift of the dominant phytoplankton
from Skeletonema and Chaetoceros to Chaetoceros and Nitzschia all of which belong to Bacillariophyceae. Hence, we evaluated Nitzschia, together with Chaetoceros and Isocrysis, as food for the oyster. Wet weights, lengths, widths, glycogen contents, and growth rates in terms of wet weight of the
oysters in all the feeding treatments were significantly higher than those in the non-feeding treatment. The highest glycogen
content (2.34%) and growth rate (2.21 g month−1) were found in the Chaetoceros treatment. Growth rate in the Isocrysis treatment (1.63 g month−1) was also high, although glycogen content in this treatment (0.41%) was low. In the Nitzschia treatment, growth rate of the oyster (0.94 g month−1) was the lowest and glycogen content (0.83%) was also low relative to that in the Chaetoceros treatment. Chlorophyll a concentration in fecal pellets was lowest in the Nitzschia treatment (<2.7 μg mg−1), suggesting more complete digestion of Nitzschia by the oyster. Thus, Nitzschia was edible and digestible but not assimilated by P. fucata. We propose the following scenario for the relationship between Nitzschia dominance and mass mortality. When Nitzschia dominates in a culture area, the physiological condition of P. fucata deteriorates due to low assimilation of Nitzschia by the oyster, followed by susceptibility of the oyster to infection by agents lethal to the oyster. 相似文献
106.
Muzaffer Mustafa Harlioğlu 《Aquaculture International》2008,16(4):351-360
This review focuses on the present distribution of populations of the crayfish Astacus leptodactylus that are harvested in Turkey. It also examines the history of this harvest and the impact that crayfish plague has had on them. Crayfish plague, caused by the fungus-like organism, Aphanomyces astaci Schikora, 1906, is a severe parasite of freshwater crayfish and has caused a lot of damage to A. leptodactylus populations in Turkey since 1984. Turkey was the largest provider of A. leptodactylus to Western Europe from 1970 (or possibly earlier) until 1986. For example, the peak production was reached in the early 1980s, with over 5,000 tonnes being exported in 1984. On the other hand, as a result of the crayfish plague the harvest of A. leptodactylus was reduced severely in most populations in Turkey after 1985. The harvest was only 320 tonnes in 1991. After the occurrence of crayfish plague in Turkey, in order to increase crayfish production uncontrolled A. leptodactylus stockings have been carried out in many waterbodies throughout Turkey. These introductions have caused an increase in the number of A. leptodactylus populations, but exploitation of A. leptodactylus is still under the pressure of the plague, although there has been a steady increase in crayfish production in recent years. The harvest increased to 2,317 tonnes in 2004. Fortunately, among those populations affected by crayfish plague, large amounts of A. leptodactylus can still be harvested from three lakes, ?znik (Bursa), E?irdir (Isparta) and Çivril (Denizli). Thus, it seems that A. leptodactylus has a degree of resistance to crayfish plague. It is therefore interesting to investigate the resistance of A. leptodactylus caught from these populations to crayfish plague. 相似文献
107.
Youji Wang Menghong Hu Weimin Wang Ling Cao Yi Yang Biping Lü Rongrong Yao 《Aquaculture International》2008,16(6):539-549
The diel feeding rhythm and ontogenesis during early life stage of loach Misgurnus anguillicaudatus were investigated under experimental conditions (light: L 06:00–18:00, D 18:00–06:00 h). Morphological and behavioral developments
of loach from newly hatched to 40 days after hatching were observed. Larvae were able to prey on daphnia 3–4 days after hatching
at 23 ± 0.5°C. As the larvae grew, they showed an increasing feeding capacity and a distinct feeding rhythm. Feeding intensity
and incidence for day-4 larvae were highest at 10:00 and 16:00 h. The highest levels of feeding intensity for day-12 larvae
occurred at 08:00, 12:00, and 18:00 h as did feeding incidence. By day 20, when the larvae metamorphosed, the highest levels
of feeding intensity occurred at 06:00, 18:00, and 24:00 h and were concurrent with the highest feeding incidence. After metamorphosis,
feeding capacity had again increased considerably and, in contrast to the earlier stages before day 20, feeding intensity
for day-30 juveniles peaked at 05:00 and 20:00 h, about 1–2 h after the maximum feeding incidence. The feeding rhythm of loach
juveniles at day 40 was almost the same as the day-30 juveniles. The estimated maximum daily feeding rates were 43.1%, 33.4%,
19.0%, 12.8%, and 5.8% of body weight on days 4, 12, 20, 30, and 40, respectively. Thus, loach was found to have different
feeding rhythms in the pre- and post-metamorphosis stages, with the highest feeding activity in daytime during the larval
planktonic stage before metamorphosis, and intensely nocturnal feeding behavior during the juvenile benthic stage after metamorphosis. 相似文献
108.
To determine whether olive flounder (Paralichthys olivaceus) immunized with lysate of Philasterides dicentrarchi could be protected immobilization antigen (i-antigen) type independently, fish were immunized with lysate of ciliates obtained from in vitro cultures or ciliates obtained from infected fish. Fish immunized with ciliates obtained from infected fish were completely protected against artificial infection with in vitro cultured P. dicentrarchi, in spite of a weak or no serum agglutination activity against in vitro cultured ciliates. The present results indicate that i-antigen-independent protection was elicited by immunization of fish with the ciliates lysate, and the cultured ciliates would provide a good source for preparation of vaccines, which are cross-protective against various i-antigen types of P. dicentrarchi. 相似文献
109.
Rahul Navik Faizan Shafiq Asfandyar Khan Manoj Datta Xiongyi Peng Md. Kamruzzaman Yingjie Cai 《Fibers and Polymers》2017,18(6):1115-1123
Wool fabric was treated with liquid ammonia at -40 °C for 30 and 60 s prior to the application of polypyrrole (PPy). The polymer was deposited on wool fiber using the chemical oxidation method with 0.02 and 0.05 mol/l (Py) monomer concentration and FeCl3 as a catalyst. Functional groups of wool samples were analyzed using FT-IR, and surface morphology was investigated using SEM micrographs. Properties such as water absorbency, surface resistivity, abrasion resistance, weight add-on, and air permeability of coated specimens were explored. The FT-IR outcomes revealed the liquid ammonia pre-treatment changed the amount of amide I (NH), cystic acid, cystic monoxide, and dioxide content of the fiber. SEM micrographs revealed the descaling of wool surface after pre-treatment and smooth coating of polymer. Pre-treatment of wool in liquid ammonia improved absorbency of wool fabric with respect to the treatment duration. The surface resistivity of wool fabric decreased with the increase of monomer concentration and pre-treatment duration. The results of abrasion resistance confirmed that the pre-treated fabric exhibited lower loss of polymer after 200 cycles of abrasion. The weight of the fabric was increased and air permeability decreased when the monomer concentration and liquid ammonia pre-treatment duration was increased. 相似文献
110.
Khadija M. Zadeh I. M. Inuwa Reza Arjmandi Azman Hassan M. Almaadeed Zurina Mohamad P. Noorunnisa Khanam 《Fibers and Polymers》2017,18(7):1330-1335
This work investigated the effects of date palm leaf fiber (DPLF) content on the thermal and tensile properties; and morphology of compatibilized polyolefin ternary blend. Recycled polyolefin ternary blend consisting of low density polyethylene (RLDPE), high density polyethylene (RHDPE) and polypropylene (RPP) were fabricated at different parts per hundred resin (phr) of DPLF. Maleic anhydride grafted polyethylene (MAPE) was used as compatibilizer to enhance the adhesion between filler and polymer matrix. The composites were prepared using melt extrusion and tests samples were produced via injection molding process. Thermal conductivity results showed that as much as 11 % reduction in thermal conductivity was achieved with the incorporation of 30 phr DPLF. Highest tensile strength was observed with the incorporation of 10 phr DPLF. The elongation at break was reduced with the addition of DPLF due to impediment of chain mobility by the fillers. Initial degradation temperature increased with the addition of DPLF. Hence, it is concluded that DPLF can be used to develop green and thermally insulating composites. It is hoped that the present results will stimulate further studies on the thermally insulative materials based on natural fibers reinforced polymer composites for applications in the building industries. 相似文献