Prions represent the active agent in transmissible spongiform encephalopathy (TSE) diseases and can remain infective to mammals even after prolonged periods in soil. The influence of mesofauna on prion dispersal and degradation in soil, however, remains unknown. In this study the effect of earthworms on the retention/dissemination of TSEs in soil was evaluated using a model recombinant prion protein (recPrP) and aqueous extracts from soil and fresh casts of two earthworm species, Lumbricus terrestris and Aporrectodea caliginosa. Our results showed that earthworm gut-derived enzymes did not enhance the degradation of recPrP in comparison to soil, even though non-prion related proteolytic activity was higher in fresh worm excrements than in soil samples. Complete degradation of recPrP occurred in the aqueous extracts from all samples within up to 6 days at +15 °C. The proteolytic enzymes responsible for degrading recPrP were inhibited by aprotinin and leupeptin and studies in pure cultures suggested these were most probably of soil microbial origin. 相似文献
Summary Plants grown from seed with high (1.5–7.3 g Mo seed-1) and low (0.07–1.4 g Mo seed-1) Mo contents were grown in the presence and absence of Mo in growth media (perlite) or in a flowing-solution culture, in a controlled environment. Neither the high (1.5 g Mo seed-1) nor the low (0.1 g Mo seed-1) Mo content in seed from a small-seeded genotype (BAT 1297) was able to prevent Mo deficiency (reduced shoot, root and nodule dry weight, N2 fixation and seed production) in growth media without an external supply of Mo, whereas both the high (7.3 g Mo seed-1) and the low (0.07 g Mo seed-1) contents in seed were able to prevent Mo deficiency in a large-seeded genotype (Canadian Wonder). Responses to Mo treatment by the Two genotypes were inconsistent between the growth media and solution culture experiments. Seed with a large Mo content (3.5 g Mo seed-1) from the Canadian Wonder genotype was unable to prevent Mo deficiency (reduced shoot and nodule dry weight and N2-fixation) in a solution culture without an external source of Mo, whereas both the large (1.7 g Mo seed-1) and the small (0.13 g Mo seed-1) contents in seed prevented a deficiency in BAT 1297. Growing plants from seed with a small Mo content, without additional Mo, reduced the seed Mo content by 83–85% and seed production by up to 38% in both genotypes. Changes in seed size and increases in shoot, root and nodule dry weight occurred, but varied with the genotype and growth conditions. These effects were also observed in some cases where plants were grown with additional Mo, demonstrating that the amount of Mo in the seed sown can influence plant nutrition irrespective of the external Mo supply. Nodule dry weight, total N content of shoots and seed production were improved by using seed with a small Mo content (1.64–3.57 g Mo seed-1) on acid tropical soils in Northern Zambia. Plants of both the large- and small-seeded genotypes grown from seed with a small Mo content (<1.41 g Mo seed-1) had a smaller nodule weight, accumulated less N and produced less seed. The viability of seed with a small Mo content was lower (germination up to 50% less) than that of seed with a large Mo content. 相似文献
The need to prioritize species based on their perceived endangerment has led to the development of systems for categorizing and assessing their degree of vulnerability. Systems with divergent biological and geographical scopes can result in conflicting lists of high-priority species, potentially confusing conservationists and hampering the efficient allocation of resources. To assess conservation priorities for North American birds, we compare three priority-setting systems; those of the International Union for the Conservation of Nature (IUCN), NatureServe, and Partners in Flight (PIF). We found highest correspondence among the three systems in the highest and lowest categories of the respective systems with lower levels of correspondence in intermediate categories. We suggest that this is because the systems, while using different formulations of criteria, are based on the major factors known to be correlated with extinction risk. The few examples of species listed as a high conservation priority by one group but not one or both of the others appear to be the result of differences in availability or interpretation of data. Better communication and collaboration among those responsible for compiling the priority lists for each system is needed.A primary difference among the systems was the total number of species identified as conservation priorities. IUCN identified the fewest species (47) and PIF the most (157). This difference is the result of differences in geographic and taxonomic scope of each system. However, when considered as the percent of the total number of taxa evaluated by each system, all systems identified approximately 20% of species as of conservation concern. To reconcile disparate lists, we urge that conservationists use a hierarchical approach that first considers species that meet thresholds for endangerment under global systems, followed by species considered most vulnerable relative to all continental biota. 相似文献
In contaminated streams, understanding the role of streambank and streambed source contributions is essential to developing robust remedial solutions. However, identifying relationships can be difficult because of the lack of identifying signatures in source and receptor pools. East Fork Poplar Creek (EFPC) in Oak Ridge, TN, USA received historical industrial releases of mercury that contaminated streambank soils and sediments. Here, we determined relationships between the contaminated streambank soils and sand-sized streambed sediments.
Materials and methods
Field surveys revealed the spatial trends of the concentrations of inorganic total mercury (Hg) and methyl mercury (MeHg), Hg lability as inferred by sequential extraction, particle size distribution, and total organic carbon. Statistical tests were applied to determine relationships between streambank soil and streambed sediment properties.
Results and discussion
Concentrations of Hg in streambank soils in the upper reaches averaged 206 mg kg?1 (all as dry weight) (n?=?457), and 13 mg kg?1 in lower reaches (n?=?321), while sand-sized streambed sediments were approximately 16 mg kg?1 (n?=?57). Two areas of much higher Hg and MeHg concentrations in streambank soils were identified and related to localized higher Hg concentrations in the streambed sediments; however, most of the streambank soils have similar Hg concentrations to the streambed sediments. The molar ratio of Hg to organic carbon, correlation between MeHg and Hg, and particle size distributions suggested similarity between the streambank soils and the fine sand-sized fraction (125–250 μm) collected from the streambed sediments. Mercury in the fine sand-sized streambed sediments, however, was more labile than Hg in the streambank soils, suggesting an in-stream environment that altered the geochemistry of sediment-bound Hg.
Conclusions
This study revealed major source areas of Hg in streambank soils, identified possible depositional locations in streambed sediments, and highlighted potential differences in the stability of Hg bound to streambank soils and sediments. This work will guide future remedial decision making in EFPC and will aid other researchers in identifying source–sink linkages in contaminated fluvial systems.
The chemical and biological composition of groundwater sampled from a 76 m deep borehole situated 80 m from an unlined, earth-banked lagoon excavated in Upper Chalk and continuously used to store liquid cow manure was regularly monitored from August 1975 to January 1982 for signs of pollution. The lagoon became self-sealing 3 months after the start of filling in 1975, but was further enlarged and re-filled in autumn 1976, and emptied by dragline in September 1978 and 1980. No significant changes in groundwater composition were detected in the period August 1975 to December 1978. In January 1979, significant numbers of Escherichia coli and a large, rapid increase in concentrations of ammonium-nitrogen (NH4-N), phosphorus (P), potassium (K), sodium (Na), chloride (Cl) and sulphate (SO4) were detected in the groundwater. Nutrient concentrations fluctuated at elevated levels throughout 1979 but decreased during 1980. Additional bacterial and chemical (NH4-N, P and K) contamination was recorded in January 1981 and subsequent months. The contamination was considered to have occurred as a result of fissure flow through the unsaturated zone of the Upper Chalk following repeated disruption of the self-sealing layer during emptying of the lagoon by dragline in September 1978 and 1980. The results indicate that unlined, earth-banked slurry storage lagoons need to be carefully managed to avoid biological and chemical pollution of Chalk aquifers. 相似文献
Seeds of cabbage, lettuce, millet, radish, turnip, and wheat were treated with solutions containing Be, Ni, Tl, or V, and subsequent effects on seed germination and radicle elongation were measured after three days. Treatment with low concentrations of Be, Ni, or V stimulated root elongation in most species. Higher concentrations of these elements and all treatment with Tl caused reductions in root elongation. In general, turnip and lettuce were the most sensitive of the plants studied to the metals tested, while wheat and millet were the least sensitive. 相似文献
The effect of inulin on isoflavone absorption and the effect of isoflavones and synergy with inulin on calcium absorption in rats was investigated. Rats (n = 48) were divided into three groups and fed inulin (50 mg/g), isoflavone (8 mg/g) or inulin + isoflavone (50 mg/g + 8 mg/g) diets for 21 days. After a 2-h fast, rats were given (45)Ca orally or intraperitoneally, together with 25 mg of calcium as calcium acetate. Blood and femurs were collected 4 days later. Sera were analyzed for isoflavones using HPLC-MS, femurs for (45)Ca by beta-scintillation counting, and total femoral calcium by atomic absorption spectroscopy. Both groups fed isoflavones had similar and significantly higher weight-adjusted total femoral calcium content compared to the inulin-fed group (p < 0.0001). (45)Ca absorption was significantly higher (p < 0.01) when isoflavones were added to the diet, and serum equol was significantly lower (p < 0.01) when inulin was added to the diet containing isoflavones. We conclude that isoflavones enhance calcium absorption, without synergy from inulin, and that inulin decreases equol production. 相似文献
Processes that govern the soil nitrogen (N) supply in irrigated lowland rice systems are poorly understood. The objectives
of this paper were to investigate the effects of crop rotation and management on soil N dynamics, microbial biomass C (CBIO) and microbial biomass N (NBIO) in relation to rice N uptake and yield. A maize-rice (M-R) rotation was compared with a rice-rice (R-R) double-cropping
system over a 2-year period with four cropping seasons. In the M-R system, maize (Zea mays L.) was grown in aerated soil during the dry season (DS) followed by rice (Oryza sativa L.) grown in flooded soil during the wet season (WS). In the R-R system, rice was grown in flooded soil in both the DS and
WS. Three fertilizer N rates (0, 50 or 100 kg urea-N ha–1 in WS) were assigned to subplots within the cropping system main plots. Early versus late crop residue incorporation following
DS maize or rice were established as additional treatments in sub-subplots in the second year. In the R-R system, the time
of residue incorporation had a large effect on NO3–-N accumulation during the fallow period and also on extractable NH4+-N, rice N uptake and yield in the subsequent cropping period. In contrast, time of residue incorporation had little influence
on extractable N in both the fallow and rice-cropping periods of the M-R system, and no detectable effects on rice N uptake
or yield. In both cropping systems, CBIO and NBIO were not sensitive to residue incorporation despite differences of 2- to 3-fold increase in the amount of incorporated residue
C and N, and were relatively insensitive to N fertilizer application. Extractable organic N was consistently greater after
mid-tillering in M-R compared to the R-R system across N rate and residue incorporation treatments, and much of this organic
N was α-amino N. We conclude that N mineralization-immobilization dynamics in lowland rice systems are sensitive to soil aeration
as influenced by residue management in the fallow period and crop rotation, and that these factors have agronomically significant
effects on rice N uptake and yield. Microbial biomass measurements, however, were a poor indicator of these dynamics.
Received: 31 October 1997 相似文献
Micrometeorological measurements of nighttime ecosystem respiration can be systematically biased when stable atmospheric conditions lead to drainage flows associated with decoupling of air flow above and within plant canopies. The associated horizontal and vertical advective fluxes cannot be measured using instrumentation on the single towers typically used at micrometeorological sites. A common approach to minimize bias is to use a threshold in friction velocity, u*, to exclude periods when advection is assumed to be important, but this is problematic in situations when in-canopy flows are decoupled from the flow above. Using data from 25 flux stations in a wide variety of forest ecosystems globally, we examine the generality of a novel approach to estimating nocturnal respiration developed by van Gorsel et al. (van Gorsel, E., Leuning, R., Cleugh, H.A., Keith, H., Suni, T., 2007. Nocturnal carbon efflux: reconciliation of eddy covariance and chamber measurements using an alternative to the u*-threshold filtering technique. Tellus 59B, 397–403, Tellus, 59B, 307-403). The approach is based on the assumption that advection is small relative to the vertical turbulent flux (FC) and change in storage (FS) of CO2 in the few hours after sundown. The sum of FC and FS reach a maximum during this period which is used to derive a temperature response function for ecosystem respiration. Measured hourly soil temperatures are then used with this function to estimate respiration RRmax. The new approach yielded excellent agreement with (1) independent measurements using respiration chambers, (2) with estimates using ecosystem light-response curves of Fc + Fs extrapolated to zero light, RLRC, and (3) with a detailed process-based forest ecosystem model, Rcast. At most sites respiration rates estimated using the u*-filter, Rust, were smaller than RRmax and RLRC. Agreement of our approach with independent measurements indicates that RRmax provides an excellent estimate of nighttime ecosystem respiration. 相似文献
The almond major storage protein, amandin, was prepared by column chromatography (amandin-1), cryoprecipitation (amandin-2), and isoelectric precipitation (amandin-3) methods. Amandin is a legumin type protein characterized by a sedimentation value of 14S. Amandin is composed of two major types of polypeptides with estimated molecular weights of 42-46 and 20-22 kDa linked via disulfide bonds. Several additional minor polypeptides were also present in amandin. Amandin is a storage protein with an estimated molecular weight of 427,300 +/- 47,600 Da (n = 7) and a Stokes radius of 65.88 +/- 3.21 A (n = 7). Amandin is not a glycoprotein. Amandin-1, amandin-2, and amandin-3 are antigenically related and have similar biochemical properties. Amandin-3 is more negatively charged than either amandin-1 or amandin-2. Methionine is the first essential limiting amino acid in amandin followed by lysine and threonine. 相似文献