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901.
Yan Tan Kiyoshi Osatomi Yukinori Nozaki Tadashi Ishihara Kenji Hara 《Fisheries Science》2006,72(1):185-194
ABSTRACT: We purified cathepsins B1 and B2 from the ordinary muscle of carp Cyprinus carpio . The N-terminal amino acid sequences (12 residues) of 29 kDa bands of cathepsins B1 and B2 are the same and showed high homology of 75% and 83%, respectively, with the heavy chain of rat and human cathepsins B. Based on conserved sequences of other cathepsins B and the N-terminal amino acid sequences of 29 kDa bands, we cloned carp cathepsin B cDNA. The nucleotide sequence of carp cathepsin B cDNA consists of 1470 bp including a 993 bp open reading frame, encoding a deduced protein of 330 amino acids. The deduced amino acid sequence of carp cathepsin B has similarity of 80% to rainbow trout cathepsin B and of 76–78% to other vertebrate cathepsins B. The sequence of its isoform was also determined during molecular cloning, which has 94.8% similarity with first cloned cathepsin B. They are completely same in N-terminal amino acid sequence of heavy chain, active site and potential N-glycosylation site. This indicates there are at least two kinds of cathepsin B functioning in vivo in carp. 相似文献
902.
Wenresti G Gallardo Atsushi Hagiwara Kenji Hara Kiyoshi Soyano 《Fisheries Science》2006,72(4):781-786
ABSTRACT: A growth hormone (GH)-like substance was extracted from the rotifer Brachionus plicatilis and subsequently purified by gel filtration and ion exchange chromatography. The GH-like substance had a molecular weight of approximately 28 kDa and had cross-reactivity with salmon GH antibody. In vivo bioassay showed a higher intrinsic rate of increase and net reproduction rate of B. plicatilis treated with the GH-like substance. 相似文献
903.
Taeko Miyazaki Jun Kohbara Kenji Takii Yasunori Ishibashi Hidemi Kumai 《Fisheries Science》2008,74(2):314-321
ABSTRACT: In bluefin tuna aquaculture, collision of juveniles with the tank or net walls is a major cause of high mortality. This problem may be related to color sensibility of the visual mechanisms of this species. As a first step in understanding of color vision of Pacific bluefin tuna Thunnus orientalis , we applied a molecular technique and histology to study cone cell distribution in the retina of juvenile fish. We isolated three cone opsin genes encoding one blue-sensitive ( SWS2 ) and two green-sensitive ( RH2 ) visual pigments. In situ hybridization revealed that SWS2 mRNA is localized in the single-cone photoreceptors. The localization of the two RH2 signals in distinct cone cells was not determined, probably because of the high homology between the two RH2 genes. Single-cone photoreceptors appeared frequently in the ventral–temporal retina in approximately 80-mm fish and in the temporal retina in approximately 230-mm fish. These cone distributions may define a visual field with best color contrast vision in front and above the fish with a short wavelength (blue) reflecting target (sensed by single cones), and may be enhanced against the longer wavelength (green) background when fish see a target below them (sensed by double cones). 相似文献
904.
Ichiro?Nagano Seiko?Inoue Kenji?Kawai Syun-ichirou?OshimaEmail author 《Fisheries Science》2009,75(3):707-714
Pseudotuberculosis is a bacterial septicaemia caused by Photobacterium damselae subsp. piscicida in several marine fish species. Yellowtail Seriola quinqueradiata is the most sensitive fish species to this disease. The internal organs of naturally infected yellowtail exhibit whitish
spots, tubercle-like tissue structures, consisting of bacterial accumulations. There have been many trials for experimental
infection, however adequate method of infection that reproduces moderate mortality and primary clinical signs has not yet
established. Present investigation evaluated an immersion infection method by using logarithmic culture-phase bacteria resulting
in higher mortality than that using stationary culture-phase bacteria. Typical white spots on the spleen and kidney were also
observed constantly in dead fish. Transmission electron microscopy and fluorescent antibody microscopy showed bacterial clusters
not only in the spleen and kidney but also in the blood channels in the secondary gill filaments. These results were confirmed
repeatedly by plural experiments. The use of logarithmic-phase bacteria in immersion infection is an appropriate technique
to reproduce moderate mortality and primary clinical signs, which will be a reliable infection method also for the challenge
test of pseudotuberculosis vaccine. 相似文献
905.
Warita K Sugawara T Yue ZP Tsukahara S Mutoh K Hasegawa Y Kitagawa H Mori C Hoshi N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1257-1267
For the purpose of investigation of working mechanisms in endocrine disruptors, we evaluated the dose-related effects of fetal and/or neonatal exposure to an estrogenic compound on the male reproductive organs in adult mice, particularly with respect to gene expression of steroidogenic acute regulatory protein (StAR). The pregnant ICR mice were given subcutaneous injections of 10 micro g/day/animal of diethylstilbestrol (DES) to subject the fetal mice to in utero exposure (IUE). Subsequently, the newborn male mice were subjected to neonatal exposure (NE) by treatment with vehicle or 0.1-10 micro g/day/animal of DES. Fertility rates of each group were as follows: control, 100%; IUE only, 60%; IUE+NE 0.1 micro g, 25%; IUE+NE 1 micro g, 0%; IUE+NE 10 micro g, 0%. In general histology, germ cell layers in the seminiferous tubules were thinned in the group of IUE+NE 10 micro g. Hypoplasia of the Leydig cells, in which the staining intensity of eosin was diminished, was also observed in the groups of IUE+NE 0.1-10 micro g. The androgen receptor (AR) and estrogen receptor alpha (ERalpha) immunoexpression in the Leydig cells of IUE+NE 1-10 micro g was slightly lower than that in the controls. Long-term dysfunction of the hypothalamo-pituitary-testicular axis, including sustained hypoproduction of gonadotropin and testosterone, and altered expressions of steroid hormone receptors and StAR genes were observed. The hypothalamo-pituitary control of gonadotropin secretion may be affected by the smaller doses of estrogenic agents than the reproductive organs. Furthermore, the fertility rate in the male mice exposed to this estrogenic agent was closely correlated with the testosterone levels, and even more so with the rate-limiting factor of steroidogenesis, StAR. This finding suggests that endocrine disruptors have an important pronounced effect on StAR gene expression. 相似文献
906.
Kenji Rimpo Miyuki Hirabayashi Aki Tanaka 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(4):1390
BackgroundMiniature Dachshunds (MD) are predisposed to lymphoma with disease onset of young age and long‐term survival.ObjectivesTo compare clinical features and survival time of lymphoma in MD and non‐MD.AnimalsOne hundred and eight MDs with lymphoma and 149 non‐MD breed dogs with lymphoma were included in the study.MethodsThis was a retrospective multicenter observational study. Lymphoma was classified based on signalment, histopathology/cytology, and anatomical site of the disease. For each type of lymphoma, median survival time was analyzed by Kaplan‐Meier estimates and life table analysis. Prognostic factors for large‐cell gastrointestinal lymphoma (LGIL) were analyzed using Cox regression.ResultsGastrointestinal lymphomas were more common in MDs (53/108) compared to non‐MDs (41/149). The multicentric lymphoma was most common in non‐MD breed dogs (74/149) compared to MDs (33/108). The median age that dog developed lymphoma in MD and non‐MD were both 10 years old; however, lymphomas were more frequently observed in younger dogs (<4 years) in MDs (20/108) compared to non‐MDs (9/149; P = .002). Seventy percent were diagnosed with B‐cell with median age of diagnosis was 3 (1‐14) years. Mott cell differentiation was observed in 6 dogs. Age <4 years and B‐cell phenotype were significant factors for longer survival time in MD with LGIL.Conclusions and Clinical ImportanceLymphomas in MDs involved gastrointestinal lesions at higher frequency compared to other dog breeds examined. B‐cell lymphoma was more common in early‐onset LGIL in MD and cases that involved Mott cell differentiation were observed. Awareness of this specific presentation of lymphoma in dogs will possibly affect the treatment decision process for the owners of MD with LGIL. 相似文献
907.
Hirohito OGAWA Kenji OHYA Raphael AYIZANGA Hiroko MIYAMOTO Asako SHIGENO Masao YAMADA Yasuhiro TAKASHIMA Miho INOUE-MURAYAMA Ayato TAKADA Boniface BABOREKA KAYANG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1491
Some filoviruses such as ebolaviruses and marburgviruses, cause hemorrhagic fever in humans and nonhuman primates. Pigs are suggested to play a potential role in the filovirus ecology. We investigated the seroprevalence of filovirus infection in pigs in Ghana. Using a viral glycoprotein (GP)-based enzyme-linked immunosorbent assay, we detected filovirus-specific immunoglobulin G antibodies in 5 of 139 samples. These positive sera showed specificities to four different filovirus species. Particularly, two of the positive sera reacted to GPs of two African ebolaviruses (i.e., Ebola virus and Taï Forest virus) in Western blotting. Our results suggest that these Ghanaian pigs were exposed to multiple filoviruses and emphasize the importance of continuous monitoring of filovirus infection in pig populations in West African countries. 相似文献
908.
Ryuichi Haginoya Kunio Sakai Takashi Komatsu Seiichi Nagao Kenji Yokoyama Toshifumi Takeuchi Ritsuko Matsukawa Isao Karube 《Cereal Chemistry》1997,74(6):745-749
The determination of damaged starch and diastatic activity in flour was studied using a flow-injection analysis (FIA) biosensor system. The system consisted of an oxygen electrode and an immobilized enzyme column containing purified glucoamylase and glucose oxidase immobilized on activated aminopropyl glass beads. The biosensor system has an optimum pH between 6.5 and 7.5 and an optimum temperature of 35°C for glucose measurement. The response of the FIA biosensor was linear up to 1.000 g/L of glucose with a lower detection limit of 0.025 g/L. Each assay took about 20 min, and the system showed good reproducibility (r = 0.998, n = 8). When applied to the measurement of damaged starch and diastatic activity in wheat flour, the results obtained agreed with those obtained using the conventional methods of measurement. This biosensor system is a rapid practical alternative for the measurement of damaged starch and diastatic activity in wheat flour. 相似文献