全文获取类型
收费全文 | 121篇 |
免费 | 5篇 |
专业分类
林业 | 1篇 |
农学 | 5篇 |
31篇 | |
综合类 | 3篇 |
农作物 | 5篇 |
水产渔业 | 24篇 |
畜牧兽医 | 40篇 |
园艺 | 1篇 |
植物保护 | 16篇 |
出版年
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 5篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 4篇 |
2014年 | 8篇 |
2013年 | 14篇 |
2012年 | 6篇 |
2011年 | 7篇 |
2010年 | 6篇 |
2009年 | 3篇 |
2008年 | 7篇 |
2007年 | 5篇 |
2006年 | 8篇 |
2005年 | 9篇 |
2004年 | 10篇 |
2003年 | 8篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1995年 | 1篇 |
1989年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有126条查询结果,搜索用时 15 毫秒
101.
Ike K Takeuchi K Uchida Y Imai S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):457-460
Hematological findings during the course of infection and the antibody response in Syrian hamsters infected with Babesia microti were examined. A macrocytic hypochromic anemia with an increase of the reticulocyte count was detected as a rise in the parasitized erythrocyte rate. White blood cell counts also remarkedly increased with the increases of both neutrophils and active-shaped monocytes, and thus they particularly play an important role in eliminating the parasite. In Western blotting with the sera from the hamsters infected with B. microti, a 38 kDa protozoan antigen reacted to the early-term sera, and additionally 28, 32, and 34 kDa antigens also reacted to the medium- and latter-term, and convalescent sera. These antigens were immunodominant and the antibodies against these antigens had also important roles for inhibition of this parasite. 相似文献
102.
103.
Seiji TSUGE Ayako FURUTANI Rie FUKUNAKA Takashi OKU Kazunori TSUNO Hirokazu OCHIAI Yasuhiro INOUE Hisatoshi KAKU Yasuyuki KUBO 《Journal of General Plant Pathology》2002,68(4):363-371
To analyze the regulation of hrp expression and to detect and identify hrp-dependent secretion proteins of plant-pathogenic bacteria, an appropriate hrp-inducing medium is indispensable. In this study, two efficient hrp-inducing media for Xanthomonas oryzae pv. oryzae were designed by assaying the expression of a hrcU (the first gene of the hrpC operon) and a gus (β-glucuronidase) fusion gene. We modified XVM2, which is a hrp-inducing medium for X. campestris pv. vesicatoria, by adding 0.01% xylose in place of fructose and sucrose (0.18 and 0.34%, respectively) as a sugar source. The resulting
medium induced approximately 15-fold more GUS activity from transformants containing a hrcU::gus gene than did XVM2. Moreover, a methionine-containing synthetic medium with 0.18% xylose as a sugar source was able to induce
much stronger expression of HrcU::GUS, with GUS activity approximately 100-fold greater than that in XVM2. Induction depended
on a regulator, HrpXo, and the PIP (plant-inducible-promoter) box, suggesting that HrcU::GUS was expressed in a hrp-dependent manner. The induction of operons hrpA to hrpF in XOM2 was also confirmed. These results suggest that both media, especially XOM2, are highly efficient hrp-inducing media for X. oryzae pv. oryzae.
Received 7 October 2002/ Accepted in revised form 22 November 2002 相似文献
104.
The present study investigated the influence of feeding a large amount of grass hay to steers from the early to middle fattening period on growth, carcass characteristics, and meat characteristics. Steers were randomly divided into grass hay‐fed (GHF, n = 6) and concentrate‐fed (CF, n = 6) groups. The dressed weight of the GHF steers was lower than that of the CF steers, but the final body weight was not significantly different between the groups. The GHF steers had decreased subcutaneous fat and rib thickness compared with the CF steers. Lipid content, monounsaturated fatty acids, and drip loss in the muscles were lower in the GHF steers than in the CF steers. Furthermore, n‐3 polyunsaturated fatty acids were higher in the GHF steers compared with the CF steers. The GHF steers had lower body weight during the middle fattening stage, which may have occurred as a result of muscle growth suppression caused by increased Myostatin expression; an increase in daily gain during the finishing period may have occurred as a result of muscle growth activation caused by decreased Myostatin expression. Feeding steers a grass hay‐based diet during the early fattening period possibly maintains the quantitative productivity of beef similarly to feeding a concentrate‐based diet. 相似文献
105.
Hassadin BOONSRIROJ Daria Llenaresas MANALO Kazunori KIMITSUKI Taichi SHIMATSU Nozomi SHIWA Harumi SHINOZAKI Yurika TAKAHASHI Naoto TANAKA Satoshi INOUE Chun-Ho PARK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):35-42
Rabies is a zoonotic disease caused by the rabies virus. While the salivary glands are
important as exit and propagation sites for the rabies virus, the mechanisms of rabies
excretion remain unclear. Here, we investigated the histopathology of the salivary glands
of rabid dogs and analyzed the mechanism of excretion into the oral cavity. Mandibular and
parotid glands of 22 rabid dogs and three control dogs were used. Mild to moderate
non-suppurative sialadenitis was observed in the mandibular glands of 19 of the 22 dogs,
characterized by loss of acinar epithelium and infiltration by lymphoplasmacytic cells.
Viral antigens were detected in the mucous acinar epithelium, ganglion neurons and
myoepithelium. Acinar epithelium and lymphocytes were positive for anti-caspase-3
antibodies and TUNEL staining. In contrast, no notable findings were observed in the
ductal epithelial cells and serous demilune. In the parotid gland, the acinar cells,
myoepithelium and ductal epithelium all tested negative. These findings confirmed the path
through which the rabies virus descends along the facial nerve after proliferation in the
brain to reach the ganglion neurons of the mandibular gland, subsequently traveling to the
acinar epithelium via the salivary gland myoepithelium. Furthermore, the observation that
nerve endings passing through the myoepithelium were absent from the ductal system
suggested that viral proliferation and cytotoxicity could not occur there, ensuring that
secretions containing the virus are efficiently excreted into the oral cavity. 相似文献
106.
Ogasawara H Aso H Nagai Y Matsumoto K Okamura H Tanaka S Watanabe K Ohwada S Yamaguchi T 《Domestic animal endocrinology》2008,35(3):274-280
Neuropeptide Y (NPY), a 36-amino acid member of the pancreatic polypeptide family, was found to be present by immunohistochemistry in the bovine adenohypophysis. NPY mRNA expression was confirmed in the adenohypophysis by RT-PCR. NPY immunoreactivity was present in about 38% of adenohypophyseal cells in the pars distalis. However, NPY immunoreactive cells (NPY-ir cells) were scarce in the zona tuberalis. Immunohistochemistry of NPY and specific hormones using mirror sections revealed that NPY was colocalized in GH immunoreactive cells. Over 90% of somatotrophs corresponded to NPY-ir cells. These results indicate that endogenous NPY is present in the bovine somatotroph and may act as an endocrine intercellular mediator in the adenohypophysis. 相似文献
107.
Park Jaesung Vilayvong Khonesavanh Son Younghwan Fujisawa Kazunori Murakami Akira 《Paddy and Water Environment》2019,17(3):303-313
Paddy and Water Environment - Torrential rainfall has a detrimental impact on the stability of soil-based agricultural embankments due to seepage and slope failure. The impact on the stability of... 相似文献
108.
Toshimitsu Onduka Akira Kakuno Kumiko Kono Katsutoshi Ito Kazuhiko Mochida Kazunori Fujii 《Fisheries Science》2012,78(6):1301-1308
We evaluated the acute toxicity of the antifouling biocide 2,4,5,6-tetrachloro-1,3-benzenedicarbonitrile (chlorothalonil, TPN) to an alga (Skeletonema costatum), crustacea (Tigriopus japonicus, kuruma prawn Marsupenaeus japonicus), and a teleost fish (red sea bream Pagrus major) commonly found in the coastal waters of Japan. We also performed acute toxicity and early-life-stage tests for TPN using mummichog Fundulus heteroclitus and used the results to predict the chronic toxicity of TPN to red sea bream. The acute toxicity values of TPN (based on measured concentrations) for S.?costatum, T.?japonicus, M.?japonicus, red sea bream, and mummichog were 0.95, 16, 290, 35, and 61???g/l, respectively. The early-life-stage test with mummichog embryos revealed that hatchability and growth were the most sensitive indicators, and the lowest- and no-observed-effect concentrations, based on the measured concentrations, were 32 and 11???g/l, respectively. We estimated the chronic toxicity value for red sea bream to be 11???g/l based on these toxicity values. Considering the current measured concentrations of TPN in coastal waters of Japan, its environmental impact would appear to be limited. 相似文献
109.
110.
Kazunori Minamikawa Tamon Fumoto Masayuki Itoh Michiko Hayano Shigeto Sudo Kazuyuki Yagi 《Biology and Fertility of Soils》2014,50(6):879-889
Water management practices, such as midseason drainage (MD) and intermittent irrigation, are effective in reducing methane (CH4) emission from irrigated rice paddies. In a previous study in which two-year field experiments were conducted at nine sites across Japan, prolonged MD was found to reduce the seasonal total CH4 emission by 30.5?±?6.7 % (mean?±?95 % confidence interval) compared with conventional MD without compromising rice grain yield. However, the degree of CH4 reduction by water management is dependent on prevailing weather conditions. To estimate the mean effect of prolonged MD on CH4 emission at the nine sites with rice straw application, we conducted a long-term (20 years) simulation using a process-based biogeochemistry model, the DNDC-Rice. The model adjusted using site-specific parameters was able to reproduce the measured magnitude of the total CH4 emission and the suppressive effect of prolonged MD. The number of nonrainy days during MD explained the degree of CH4 reduction for each site and all sites combined. In the simulation, mean reduction percentage was 20.1?±?5.6 % when acceptable prolonged MD (i.e., having less than 15 % yield loss) was applied compared with conventional MD. The discrepancy of the percentage between measurement and simulation was primarily attributable to longer nonrainy days during prolonged MD at several sites in the measurement than the mean of 20-year simulation. We therefore conclude that the long-term simulation better represents the mean reduction percentage of CH4 emission by prolonged MD relative to conventional MD at the nine sites across Japan. 相似文献