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11.
Toshiyuki Takai Patrick Lumanglas Daisuke Fujita Kazuhiro Sasaki Njato Michael Rakotoarisoa Yasuhiro Tsujimoto Nobuya Kobayashi Eliza Vie Simon 《Breeding Science》2021,71(5):615
The heading date is an important trait for determining regional and climatic adaptability in rice. To expand the adaptability of the indica rice cultivar ‘IR64’, we pyramided multiple early or late heading quantitative trait locus (QTLs) in the ‘IR64’ genetic background by crossing previously developed near-isogenic lines (NILs) with a single QTL for early or late heading. The effects of pyramiding QTLs were observed in three different climatic zones of the Philippines, Madagascar, and Japan. The early heading pyramiding lines (PYLs) headed 6.2 to 12.8 days earlier than ‘IR64’ while the late heading PYLs headed 18.8 to 27.1 days later than ‘IR64’. The PYLs tended to produce low grain yield compared to ‘IR64’. The low yield was not improved by combining SPIKE, which is a QTL that increases the number of spikelets per panicle. Conversely, ‘IR64-PYL(7+10)’ carrying Hd5 and Hd1 headed earlier, produced more tillers, and more panicles per m2 than ‘IR64’, and mitigated the yield decrease in early heading. These results suggest that the effects of pyramided QTLs on heading date were consistent across various environments and PYLs could be used to enhance the adaptation of ‘IR64’ in other rice growing environments. 相似文献
12.
Spake Rebecca Soga Masashi Kawamura Kazuhiro Cooke Robert S. Yamaura Yuichi Eigenbrod Felix 《Landscape Ecology》2020,35(5):1055-1071
Landscape Ecology - Functional responses to landscape heterogeneity are context-dependent, hampering the transferability of landscape-scale conservation initiatives. Japan provides a unique... 相似文献
13.
Kazuhiro Shiozaki Momo Shiozaki Junko Masuda Akiko Yamauchi Shuichi Ohwada Toshiki Nakano Toshiyasu Yamaguchi Tadao Saito Koji Muramoto Minoru Sato 《Fisheries Science》2010,76(5):865-872
Angiotensin-I-converting enzyme (ACE) plays a crucial role in the crisis of hypertension. Some peptides that originate from
protease hydrolysates are known to suppress ACE activity in vitro and in vivo. Here, we investigated whether trypsin hydrolysate
of oyster Crassostrea gigas showed hypotensive activity and ACE inhibition. The hydrolysate significantly suppressed systolic blood pressure and ACE
activity in spontaneously hypertensive rats following a one-shot oral administration and a long-term feeding experiment lasting
9 weeks. Each hydrolysate from oyster tissue showed ACE inhibitory activity, indicating the hypotensive effect was due to
synergism. One potent ACE inhibitory peptide, Asp-Leu-Thr-Asp-Tyr, was identified from the hydrolysate of the striate muscle,
and the peptide exhibited hypotensive activity in vivo. Protease digestion analysis suggested that Asp-Tyr could be the real
effector of this penta-peptide in vivo. 相似文献
14.
Hepatocyte growth factor transduces different intracellular signals in aortic and umbilical venous endothelial cells 总被引:1,自引:0,他引:1
Makondo K Kimura K Kitamura T Yamaji D Saito M 《The Japanese journal of veterinary research》2003,51(2):105-112
Endothelial cells are important for maintenance of vascular integrity by producing a variety of bioactive molecules such as nitric oxide (NO). Recent evidence has suggested that there are some differences in characteristics between endothelial cells from different origins. Here we examined responses of two typical endothelial cells to hepatocyte growth factor (HGF), which induces endothelium-dependent relaxation of microvessels. Stimulation of human umbilical vein endothelial cells (HUVEC) and bovine aortic endothelial cells (BAEC) with HGF increased endothelial NO synthase activity, accompanied with an increase of activity-related site-specific phosphorylation of protein kinase B/Akt. However, HGF stimulated phosphorylation of p38 mitogen-activated protein kinase (MAPK) only in HUVEC, but not in BAEC, while it induced phosphorylation of p44/p42 MAPK in both cells. These results suggest that HGF transduces different intracellular signals between aortic and umbilical venous endothelial cells, and that the differences might represent divergent endothelial responses to growth factors, especially those that activate receptor-tyrosine kinases. 相似文献
15.
Yasunori SHINOZUKA Naoki SUZUKI Sohei KANEKO Kazuhiro KAWAI Tomomi KURUMISAWA Yuko SHIMIZU Tadashi IMANISHI Ayumu OHNO Mano TAKAHASHI Naoki ISOBE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):310
The purpose of this study was to determine the concentrations of antimicrobial components (immunoglobulin A (IgA), lactoferrin (LF), lingual antimicrobial peptide (LAP), and S100A7) in normal milk and their relation to host factors (Age, somatic cell count (SCC), days in milk, richness, and alpha diversity of the milk microbiota) in dairy cows using multivariate regression tree analyses, and to clarify how the milk microbiota is related to the obtained results. Thirty normal milk samples were collected from a commercial dairy farm in June 2020. The thresholds that predicted the concentration of each antimicrobial component in milk were obtained by regression tree analysis, and the beta-diversity of the milk microbiota composition between groups divided according to each threshold was compared by an analysis of similarities test. The IgA and LF concentrations were mainly predicted by the SCC (177,500 and 70,000 cells/ml, respectively), and the LAP and S100A7 concentrations were predicted by Age (29.667 and 40.3 months, respectively). No relationship was observed between the concentration of IgA, LAP, or S100A7 and the milk microbiota composition between the groups divided by the threshold for prediction, but the milk microbiota composition was significantly different between the groups divided by the threshold for predicting the LF concentration. Our results indicated that the LF concentration in normal milk may be associated with the milk microbiota composition. 相似文献
16.
Yuka Maeda Keiko Nishimura Kazuhiro Kurosu Hitoshi Mizuguchi Shigeru Sato Fuminori Terada Shiro Kushibiki 《Animal Science Journal》2019,90(4):523-532
This study aimed to examine the effects of feeding kraft pulp (KP) on the growth performance, feed digestibility, and rumen fermentation of Japanese Black fattening steers. Ten Japanese Black fattening steers (aged 26 months) were randomly divided into control and KP groups. The control group (n = 5) was fed concentrate feed without KP, and the KP group (n = 5) was fed concentrate feed containing 10% KP. Both the groups were provided rice straw as roughage. The experiment was conducted over a period of 12 weeks. There was no significant difference in dry matter intake, daily body weight gain, and nutrient digestibility between both groups. No difference was observed in the ruminal concentrations of volatile fatty acids among the groups. At weeks 8 and 12 after the onset of the experiment, the acetate‐to‐propionate ratio in the ruminal fluid of the KP group was significantly higher than that of the control group. The average daily pH of ruminal fluid and activity of ruminal lipopolysaccharide did not differ between the groups. Our results suggested that the growth performance and feed digestibility in the Japanese Black fattening steers were not influenced by replacing concentrate feed with KP. 相似文献
17.
Isolation and culture of rabbit primordial germ cells 总被引:2,自引:0,他引:2
Kakegawa R Teramura T Takehara T Anzai M Mitani T Matsumoto K Saeki K Sagawa N Fukuda K Hosoi Y 《The Journal of reproduction and development》2008,54(5):352-357
Primordial germ cells (PGCs) are embryonic precursors of the gametes of adult animals and are considered stem cells of the germline. Since their proliferation in vitro correlates well with the schedule of developmental changes in vivo, they might be interesting research tools for genomic imprinting, germ-cell tumors and fertility. Furthermore, once primordial germ cells are separated and placed on a feeder layer with cytokines, they become cultured pluripotent cell lines called embryonic germ (EG) cells. EG cells share several important characteristics with embryonic stem (ES) cells as they can also contribute to the germ line of chimeras. To investigate the characteristics of PGCs and establish rabbit EG (rEG) cells, we cultured rabbit PGCs (rPGCs) in vitro with various combinations of leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and forskolin on inactivated mouse embryonic fibroblast (MEF) feeder layers. The present study found PGC proliferation in early cultures and induction of rEG-like colonies. These cells expressed pluripotent markers, such as alkaline phosphatase activity, OCT-4, Sox-2 and SSEA-1, in the undifferentiated state; however, the cells did not develop into a teratoma when injected into the kidney capsules of SCID mice, although the restricted differentiation potentials to neural cells were determined via embryoid body formation. From these characteristics and further characterization of the germ stem cell markers Vasa, SCP-1 and SCP-3, we suggested that these were hybrid cells with characteristics somewhere between PGC and EG cells. 相似文献
18.
Yamakawa K Aoki T Matsui M Yamada K Ishii M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):641-644
To establish a method to predict postpartum diseases using prepartum back fat thickness (BFT), the peripartum BFTs of 54 healthy multiparous cows before calving, which were diagnosed with postpartum displacement of the abomasum (DA), clinical ketosis or subclinical ketosis were compared with those of healthy cows from 8 weeks before the expected calving date to 8 weeks after calving. The peripartum BFTs of the cows with DA or clinical ketosis were significantly higher than those of healthy cows. The peripartum BFTs of the cows with subclinical ketosis were not significantly higher than those of the healthy cows. 相似文献
19.
Zhi-Shan Lin Zhi-Fu Cui Xiang-Yan Zeng You-Zhi Ma Zeng-Yan Zhang Toshiki Nakamura Goro Ishikawa Kazuhiro Nakamura Hisashi Yoshida Zhi-Yong Xin 《Euphytica》2007,158(1-2):109-118
Chromosome compositions of seven lines, derived from hybrids between a wheat cultivar and the wheat-Thinopyrum intermedium addition line Z6, with barley yellow dwarf virus (BYDV) resistance, were determined by genomic in situ hybridization, cytogenetic and SSR assays. The results showed that
line N522 was a disomic addition line, lines N420 and N439 were 2Ai-2(2B) chromosome substitution lines, lines N431 and N452
were 2Ai-2(2D) chromosome substitution lines, line N523 was a 2Ai-2S(2D) ditelosomic substitution line, and line N530 was
a double ditelosomic line with the mitotic chromosome number of 2n = 40 + 4t. One pair of telosomes in line N530 lacked several proximal SSR markers of chromosome 2AS, but possessed certain
terminal markers, which were consistent with an acrocentric structure, and the other pair of chromosome arms were presumably
2Ai-2S telosomes with BYDV-resistance. These wheat-Th. intermedium lines provide useful genetic resources for developing alien chromosome translocation lines. 相似文献
20.
Kanichi KUSANO Yuhiro ISHIKAWA Kazuhiro SEKI Ryo KUSUNOSE 《Journal of Equine Science》2008,19(2):25-29
Inflammatory airway disease (IAD) is a common cause of poor performance, interruption of
training and premature retirement in racehorses. It is also reported that up to 80% of
horses are affected at some point in the first years of training in UK and Australia.
However, no studies with regard to the information on occurrence of IAD in Japanese
Thoroughbred racehorses have been reported. To investigate the occurrence and the
characteristics of IAD, epidemic research including endoscopic examination of the airway
tract and trachea wash was conducted for Thoroughbred racehorses presenting coughs or poor
performance which airway tract disease was suspected stalled in training facility managed
by Japan Racing Association. Fifty-six out of 76 Thoroughbred racehorses (73.7%)
presenting coughing or poor performance were diagnosed as IAD. Mean incidence rate of IAD
was 0.3% and it has been confirmed that constant number of IAD exists in Japan. Up to
35.7% of IAD horses showed upper airway abnormalities in some extent. There was a trend
for IAD horses to use wood shavings for bedding and fed hay from the ground compared with
the control group. Therefore, improvement of stabling environment may aid in preventing
IAD. This study demonstrated that Japanese Thoroughbred racehorses are affected by IAD
likewise other countries as well as demonstrated the characteristics of IAD which may
contribute to the clarification of the pathogenesis of IAD. 相似文献