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41.
Malgorzata Witeska Piotr Sarnowski Katarzyna Ługowska Ewelina Kowal 《Fish physiology and biochemistry》2014,40(1):151-163
The effects of Cd and Cu on embryos and larvae of the ide Leuciscus idus were evaluated. The ide is an European cyprinid fish, natural populations of which tend to decrease. The ide is also used as a bioindicator organism to evaluate water quality. However, sensitivity of ide early developmental stages to heavy metal intoxication is not known. Fish were exposed to Cd or Cu (100 μg/L) during embryonic, larval or both developmental periods. Survival of the embryos, time of hatching, size and quality of newly hatched larvae were evaluated at the end of embryonic period. Correctly developed larvae from the control and Cd or Cu-exposed groups were transferred to clean water, Cd or Cu solutions (100 μg/L) immediately after hatching. Larval development was observed, and the larvae were photographed. Time of yolk sac resorption, onset of active feeding and swim bladder inflation were evaluated, and the measurements were done on body and swim bladder size. The results showed that exposure of embryos to Cd and Cu significantly reduced embryonic survival and increased frequency of body malformations and death in newly hatched larvae and delayed hatching. Exposure to Cd and Cu during larval period reduced larval survival, growth and delayed development (yolk utilization, beginning of active feeding and swim bladder inflation). Cadmium was more toxic to the ide embryos and larvae than copper. Exposures to metals during embryonic period alone caused adverse impact on larval performance even when larval development took place in clean water. However, exposure of embryos to Cu reduced toxic impact of metal on larvae in continuous Cu exposure compared to the non-preexposed fish, but no such an effect occurred in case of Cd exposure. The results show that even a short-term exposure to Cd or Cu during early development of ide may adversely affect recruitment of this species. Among the measured endpoints, quality of newly hatched larvae (frequency of body malformations and larvae dead immediately after hatching) and swim bladder size were the most sensitive to intoxication with both metals. Embryos were more sensitive to Cu intoxication than larvae, while in case of Cd, sensitivity of both stages was similar. 相似文献
42.
Krzysztof Kupren Izabela Trąbska Daniel Żarski Sławomir Krejszeff Katarzyna Palińska-Żarska Dariusz Kucharczyk 《Aquaculture International》2014,22(1):29-39
This study analyzed the morphological development and allometric growth patterns of Lota lota L. (burbot) larvae reared under controlled laboratory conditions. From hatching to day 50, twenty larvae were sampled each [between 1 and 14 days post-hatch (DPH)] or every second day (between 14 and 50 DPH) and measured under a stereoscopic microscope using analytic software. Based on the external morphology, the different stages during early development of burbot were identified: yolk sac larva 0–8 DPH [3.92–4.37 mm total length (TL)]; preflexion larva 9–26 DPH (4.57–12.06 mm TL); flexion larva (between notochord degradation and its replacement with rays) 28–34 DPH (14.00–16.34 mm TL) and postflexion larva/juvenile 36–50 DPH (18.20–29.27 mm TL). Allometric growth patterns of some parameters (e.g., total length, head length, body length, tail length, head depth, body depth, eye diameter) were modeled by a power function and described by the growth coefficient. Organogenesis and changes of body proportions in burbot larvae were more rapid and complex during the yolk sac and preflexion phase of development as larvae developed most of their sensorial, feeding, respiratory and swimming systems and after notochord flexion, when most morphological changes were related to the progressive transformation from pelagic larva to demersal larva/juvenile. 相似文献
43.
Tomasz Hura Bożena Szewczyk-Taranek Katarzyna Hura Krzysztof Nowak Bożena Pawłowska 《Water, air, and soil pollution》2017,228(2):81
The aim of this work was to analyse the response of Rosa rubiginosa to salinity induced by different concentrations of sodium chloride and calcium chloride (0, 25, 50, 100, 150 and 200 mM). Besides salt accumulation and pH changes, other parameters were investigated including photosynthetic activity, leaf water content, the dynamics of necrosis and chlorosis appearance and leaf drying. The study was complemented with microscopic analysis of changes in leaf anatomy. R. rubiginosa was more sensitive to the salinity induced by calcium chloride than by sodium chloride. Plant response to salinity differed depending of the salt concentration. These differences were manifested by higher dynamics of necrosis and chlorosis appearance and leaf drying. CaCl2 showed greater inhibition of the photosynthetic apparatus and photosynthetic activity. Treatment with CaCl2 caused more visible deformation of palisade cells, reduction in their density and overall reduction in leaf thickness. The study demonstrated higher accumulation of CaCl2 in the soil, and thus greater limitations in water availability resulting in reduced leaf water content and quicker drying of leaves as compared with NaCl-treated plants. 相似文献
44.
45.
Targońska K Kucharczyk D Zarski D Cejko BI Krejszeff S Kupren K Król R Dryl K Kowalski RK Glogowski J 《Acta veterinaria Hungarica》2011,59(3):363-372
The aim of this work was to compare the effects of controlled reproduction of cultured and wild common barbel, Barbus barbus (L.). Preparations containing different GnRH analogues and dopamine receptor antagonists (Ovopel, Ovaprim) as well as human chorionic gonadotropin (hCG) (in the case of cultured fish) were applied and their influence on ovulation, spermiation and quality of gametes obtained was determined. No differences in the qualitative or quantitative parameters of semen were found between fish stimulated with different hormonal preparations and those not receiving hormonal stimulation. The high suitability of Ovaprim for ovulation induction in (cultured and wild) barbel was confirmed. The highest synchronisation of ovulation was obtained after the application of Ovopel (18 ± 3 h), but the best results of controlled reproduction (expressed as the percentage of ovulations and survival of embryos) were obtained by applying Ovaprim (83.2 ± 4.1). A significantly higher percentage of ovulation was obtained in cultured fish (80-90%) than in wild fish (< 25%). 相似文献
46.
Renata Lebecka Jadwiga Śliwka Anna Grupa-Urbańska Katarzyna Szajko Waldemar Marczewski 《Plant pathology》2021,70(7):1745-1756
The goal of this research was to identify quantitative trait loci (QTLs) for potato tuber resistance to the soil- and seedborne bacterium Dickeya solani and for tuber starch content, to study the relationship between these traits. A resistant diploid hybrid of potato, DG 00-270, was crossed with a susceptible hybrid, DG 08-305, to generate the F1 mapping population. Tubers that were wound-inoculated with bacteria were evaluated for disease severity, expressed as the mean weight of rotted tubers, and disease incidence, measured as the proportion of rotten tubers. Diversity array technology (DArTseq) was used for genetic map construction and QTL analysis. The most prominent QTLs for disease severity and incidence were identified in overlapping regions on potato chromosome IV and explained 22.4% and 22.9% of the phenotypic variance, respectively. The second QTL for disease severity was mapped to chromosome II and explained 16.5% of the variance. QTLs for starch content were detected on chromosomes III, V, VI, VII, VIII, IX, XI, and XII in regions different from the QTLs for soft rot resistance. Two strong and reproducible QTLs for resistance to D. solani on potato chromosomes IV and II might be useful for further study of candidate genes and marker development in potato breeding programmes. The relationship between tuber resistance to bacteria and the starch content in potato tubers was not confirmed by QTL mapping, which makes the selection of genotypes highly resistant to soft rot with a desirable starch content feasible. 相似文献
47.
In Poland bread as a staple food both made from wheat and rye flour can be a potential product for future fortification with folic acid. The objective of the study was to examine the effect of fermentation and baking on added folic acid and some endogenous folates stability during breadmaking of rye and wheat breads. Breads were produced using the formulation containing enriched flour with 0.2 mg folic acid/100 g product, baker’s yeast and additionally ascorbic acid for wheat bread and lactic acid for rye bread. Folates were extracted with Hepes/Ches buffer (pH = 7.85) followed by destruction of matrix by amylase and protease and deconjugation with rat serum conjugase. Affinity chromatography (FBP bovine milk) was used to purify and concentrate samples. The folates were separated by HPLC with C18 column and with a combination of fluorescence and UV detection. For both rye and wheat breads there was a decrease of folic acid from flour to bread stage. The total losses depend on baking process and ranged from 12 to 21%. Some changes in the level of different native folate forms during the stage of baking process were also observed. 相似文献
48.
Background River and lake sediments constitute complex and difficult analytical samples. On the other hand, sediments play a fundamental
role in the distribution of toxic compounds in aquatic systems and in the evaluation of the current state and the course of
changes taking place in the environment.
Among elements present in the environment in trace concentrations, but having well-elaborated toxic properties, one of most
dangerous is arsenic. The element occurs in the environment in several chemical forms, predominant are inorganic forms of
As(V) and As(III), and methylated forms such as monomethylarsonic acid (MMAA) and dimethylarsinic acid (DMAA).
Objectives Reported herein are experiments, which were undertaken with the aim of examining the dynamics of arsenic sorption in sediments
and its dependence on speciation of the element and the pH in the environment. Simultaneously, influence of organic matter
content and chemical composition of the sediments on As sorption, were investigated.
Methods Sediment samples (upper 10 cm layer) were collected from three sites located in the vicinity of Cracow:
Using XRD and IR methods, mineral composition of sediments was analysed. Concentrations of iron, manganese, aluminium and
arsenic as well as organic matter content in solid samples were analysed.
Sorption experiment 100 g of dry sediment sample was placed in a 400 ml volume glass beaker. 300 ml of arsenic containing solution was poured
into the beaker. Every 30 min (total experiment time span equalled 7 to 7.5 hours), 0.5 ml of the suspension’ (the beaker
content was constantly mixed) was collected and centrifuged, and the concentration of arsenic was determined in the supernatant.
In the series of experiments, solution contained about 0.05 μg/ml of arsenic in organic (DMAA) and inorganic forms, at different
pH values — 3, 5, 7 and 9.
Results and Discussion Examined sediments of Vistula river and Dobczyce Lake were characterised by relatively high concentrations of arsenic, iron,
manganese and aluminium.Rudawa river. At pH 3, the concentration of inorganic As in solution decreased from an initial value of 0.049 μg/ml to 0.012 μg/ml in
7.5 hours time. The same decreasing tendency was found at pH 5 (initial value 0.046 μg/ml, after 7.5 hours - 0.015 μg/ml).
Less distinct sorption effects were noted at pH 7 and 9.
Vistula river. At all pH values, a very significant decrease of inorganic As concentration up to 80% of the initial value, as well as a
short time of achieving an equilibrium state (1 – 2.5 hours), was found.
Dobczyce lake. Inorganic As was distinctly accumulated in sediments at pH 3 (decrease of As concentration in solution from 0.052 to 0.007
μg/ml after 7.5 hours of the experiment) and at pH 5. At pH 7, about 40% of As was accumulated in sediments (solution As concentration
decrease from 0.050 to 0.030). Only 20% of As adsorbed on sediments at pH 9.Very weak accumulation of organic form of As (DMAA)
was found in all examined sediments at pH 3 and 5. At higher pH values no sorption was registered.
Conclusions The following facts were established:
Recommendations The presented method of analysis of As sorption in sediments and results visualization (based on a newly defined coefficient)
provides a useful tool for the characterization of sorptive properties of sediments and may be used for other compounds as
well. Crucial, functional information — how much of a given element may be accumulated in sediments at a certain pH — is obtained
without digestion of the sediment sample, in an experiment taking less than one day. 相似文献
• | Rudawa river — 37 km long river, flowing mainly through the suburban area; |
• | Vistula river — heavily contaminated, main Polish river; |
• | Dobczyce reservoir — artificial reservoir on Raba river, total capacity of 125,000,000 m3, supplying about 60% of drinking water to the city of Cracow. |
• | inorganic form of arsenic was effectively accumulated in all analysed sediments; |
• | inorganic As sorption in sediments strongly depended on pH — for sediments of Rudawa river and Dobczyce lake, the highest sorption was found at pH 3, and a decrease of accumulated As was noted with an increase of pH (at pH 9, the lowest amount of As was immobilised in sediments); in the case of Vistula river sediments, a reverse tendency was found; |
• | organic form of arsenic (DMAA) was accumulated only at pH 3 in all sediments and less significantly at pH 5 (Vistula river sediments); no sorption of that form of As was found in the other sediment samples; |
• | state of equilibrium (no change in inorganic As concentration in liquid medium) was achieved in 1 hour for Vistula river sediments, after about 2 hours for Rudawa river and after about 4 hours for Dobczyce lake sediments. |
49.
The aim of the present study was to explore the usefulness of serological methods in the diagnosis of postweaning multisystemic wasting syndrome (PMWS). The study was carried out in 4 PMWS-affected and 6 control farms. Based on the serological profiles, infection with porcine circovirus type 2 (PCV2) was determined to take place at 3-7 weeks of age in the PMWS-affected and at 3-11 weeks of age in the control farms. To compare the dynamics of seroconversion to PCV2 among farms, cross-sectional serological profiles were normalised in relation to the inferred age of infection. The results indicated that the proportion of seropositive pigs increased significantly slower in the affected herds. The most pronounced difference was observed about 4 weeks after infection, when the proportion of seropositive pigs ranged from 0 to 53.3% and from 70 to 100% in PMWS-affected and control herds, respectively. Mean antibody titres at that age were also significantly lower in the affected farms. These observations suggest a delay in the production of PCV2-specific antibodies and indicate that serological methods may be helpful in identifying herds with a high risk of PMWS. 相似文献
50.
Katarzyna Slizewska Adriana Nowak Zdzislawa Libudzisz Janusz Blasiak 《Research in veterinary science》2010,89(3):391-395
The aim of the study was to evaluate the influence of a probiotic preparation on the genotoxicity of faecal water of broiler chickens fed with a fodder contaminated with aflatoxin B1 (AFB1) at 1 or 5 mg per kg. Human blood lymphocytes were exposed to chicken’s faecal water samples and DNA damage was measured using the comet assay. Genotoxicity of faecal water did not depend on the AFB1 concentration in the fodder. The mean DNA damage, measured as the percentage of DNA in the tail of the comets, for chickens fed with fodder with AFB1 at 1 mg/kg was 16.80 ± 0.66, at 5 mg/kg – 16.73 ± 1.51 and in the controls – 12.79 ± 0.66. The supplementation of fodder with the probiotic preparation decreased the extent of DNA damage to 10.02 ± 0.39 for 1 mg/kg AFB1 and to 11.89 ± 0.72 for 5 mg/kg. 相似文献