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61.
中国斜纹夜蛾寄生蜂名录 总被引:10,自引:0,他引:10
根据浙江大学应用昆虫学研究所收藏的寄生蜂标本和国内外报道,对我国寄生斜纹夜蛾的原寄生蜂和重寄生蜂进行系统整理研究。我出我国斜纹夜蛾寄生蜂40种,其中包括原寄生蜂29种,重寄生蜂11种,同时还以附录的形式列出了我国已报道过但有存疑的寄生蜂7种及国外已有报道但在我国还未采集到标本的寄生蜂61种。 相似文献
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AIM: To prepare gfp-bcl-XL-contained recombinant adenovirus(rAd-gfp-bcl-XL).METHODS: Bcl-XL gene was amplified from pEGFP-C3-bcl-XL, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-XL. Then pAdTrack-CMV-bcl-XL was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-XL and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-XL into 293 cells. PCR test indicated that the recombinant Ad contained bcl-XL gene. The titer of the purified rAd-gfp-bcl-XL was 6.5×1012 PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-XL. This affords a good gene transfer vector for the gene therapy in human’s diseases. 相似文献
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AIM:To investigate the mechanism responsible for albumin microbubbles adherence to activated leukocytes. METHODS: In vitro studies were performed in which activated or nonactivated leukocytes were incubated with albumin microbubbles and observed under microscopy. The suspensions of leukocytes and microbubbles which contained or absented of integrins were analyzed with flow cytometry.RESULTS: A minimum of 50cells were identified under transillumination. 5 min after microbubbles were incubated with leukocytes, the number of cells interacting with microbubbles was greater for activated cells than for nonactivated cells(20.30±2.67 vs 4.50±1.43, P <0.01).Microbubbles attached to the surface of activated leukocytes were phagocytosed and remained intact for up to 30min. Microbubble attachment was inhibited notably by blocking the leukocyte β2-integrin Mac-1(P <0.01) and by VLA-4mAb slightly(P <0.05) CONCLUSION: The mechanism of albumin microbubbles attaching to and phagocytosed by leukocytes was due to β2-integrin and VLA-4 mediation. Phagocytosed microbubbles can remain at the regions of inflammation for15 min, also responsible to ultrasound. 相似文献
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AIM:To investigate the changes in intracellular potassium activity(aiK) and membrane potential(Vm) induced by low external sodium infusion (Low [Na]o) and to detect the mechanisms involved and the relationship between aiK and Vm. METHODS:aiK and Vm were measured in infusion with different sodium concentrations using methods of convenient and ion selective microelectrodes in guinea pig ventricular myocardium. RESULTS:Low [Na]o resulted in a decrease in aiK and an increase in Vm in a Na+ concentration-dependent manner.At the same time,contraction and resting potential increased, and action potential duration decreased markedly,but action potential amplitude was not affected. A change of the pH from 7.4 to 7.0 in low [Na]o solution reduced the decrease in aiK, but did not affect the increase in Vm.CONCLUSION:A better linear relationship appeared between the changes in aik and [Na]o or in Vm and [Na]o,while during each low [Na]o the change in both aiK or Vm may reach a new balance level. 相似文献
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调查分析稻粒黑粉病、穗颈稻瘟等两系杂交稻制种穗期主要病害的发生原因,提出了清除菌源、轮换制种、健身栽培、对口药剂防治等防治措施。 相似文献
69.
鸡腿蘑菌丝体的同工酶研究 总被引:7,自引:1,他引:7
用8种不同培养基配方,培养鸡腿蘑菌丝体,其同工酶分析结果表明,鸡腿蘑菌丝体酯酶(EST)同工酶谱差异明显,过氧化物酶(POD)同工酶谱差异明显。 相似文献
70.
淡紫拟青霉菌料防治大豆胞囊线虫的后效研究 总被引:6,自引:2,他引:6
淡紫拟青霉不同菌株的培养菌料,施225kg/hm2,对大豆胞囊线虫不但当年有45%~69%的较好防效,而且第2年和第3年仍然有22.5%~37.7%和7.3%~22.9%的后效,即空胞囊和被寄生的胞囊数量增加。 相似文献