Immunogenicity of a temperature sensitive mutant Mycoplasma gaffisepticum vaccine

The immunogenicity of the ts-11 vaccine strain of Mycoplasma gallisepticum was assessed following eye drop or coarse aerosol administration in chickens of various ages. Protection was evalualted following intra-abdominal (IA) or fine droplet aerosol administration of virulent M. gallisepticum, usually the Ap3AS strain and was measured mainly by the scoring of gross air sac lesions or by egg production. Vaccination of chickens with ts-11 did not elicit a substantial serum antibody response as measured by rapid serum agglutination test, or ELISA. Protection was never demonstrated when no M. gallisepticum serum antibody response was detected in a vaccinated group of chickens. Failure to protect occurred usually, although not invariably, following aerosol administration of the vaccine. Vaccination by eye drop usually, although not invariably provided protection against challenge. In one experiment, chickens vaccinated by eye drop at 8-weeks were as susceptible as non vaccinated controls when challenged by IA inoculation at 13-weeks-of-age. Yet other birds from the same vaccinated group were resistant when challenged in an identical way at 23-weeks. No measurable increase in M. gallisepticum specific serum antibody concentrations occurred in the intervening period. Equally surprising was the response of another group of birds in the same experiment that had been vaccinated with a higher dose of ts-11. An antibody response was detected in this group, but they were susceptible to challenge at 23-weeks. Interestingly, a drop in egg production commenced 4 weeks after challenge, 2 weeks later than that observed in a non vaccinated group challenged at the same time.(ABSTRACT TRUNCATED AT 250 WORDS)     

Relationships between electrocardiographic findings, racing performance and training in Standardbred horses

SUMMARY The presence of abnormal T waves in three or more electrocardiographic leads has been used to diagnose the cause of poor race performance in horses. This study investigated the relationship between previous racing performance in Standardbred horses and T waves, and the effect of training on the T wave. Thirty-two horses were electrocardiographed in two Sydney racing stables. Sixteen horses (50%) had ECGs with three or more leads with abnormal T waves, and these horses had won more races, had a greater ratio of wins per start and a greater number of dollars earned per start than horses with less than three abnormal T waves (P < 0.05). Horses with abnormal T waves also had significantly faster racing times (P < 0.01). There were significant (P < 0.01) correlations between the number of abnormal T waves and both number of wins (R = 0.47) and dollars earned per start (R = 0.45). Fastest winning mile rate was also negatively correlated with number of leads with abnormal T waves (R =?0.52, P < 0.01). Twenty two horses were also electrocardiographed within seven days of good racing performance. The mean number of abnormal T waves was 2.7 ± 0.41, and the mean T wave amplitude in the four chest leads (CV, CR, CL, CF) was 0.9 ± 0.15 mV. Thirteen horses (59%) had abnormal T waves in three or more leads. The effect of training on the T wave was also investigated in nine previously untrained and unraced Standardbred racehorses. There were significant effects of training (P < 0.01) on the number of abnormal T waves and mean chest lead T wave amplitude. These increases occurred in the second phase of training during which faster exercise, race trials and racing were conducted. We concluded that abnormal T waves are a common finding in the race-fit Standardbred horse, and can be found in more than 50% of horses currently performing well in races. In addition, chest lead T waves become more positive after training.     

2个多年生花生品种低温胁迫下活性氧代谢特性研究

不同低温胁迫下对平托花生Arachis pintoi与蔓花生A.duranensis2个多年生花生品种进行的叶片活性氧代谢与质膜特性进行研究。结果表明,A.duranensis品种无论在超氧化物岐化酶(SOD)、过氧化物酶(POD)还是过氧化氢酶(CAT)相对活性在低温胁迫下均不低于或显著高于A.pintoi,表现出相对较高的清除自由基能力;其质膜相对透性(RPP)增加幅度明显小于A.pintoi;丙二醛(MDA)相对含量增加率则表现出相反趋势;表明A.duranensis具有更强的抗寒性。     

汕头港海域氮、磷营养盐环境容量及排放总量控制的研究

采用带有干湿网格技术的POM08模型,对汕头港海域的二维垂向平均潮流场进行了数值模拟;同时耦合二维物质输运方程,采用分担率法计算了该海域6个人海污染源的氮、磷营养盐环境容量,并在此基础上进行了减排方案设计模拟。结果表明：水动力模拟结果与实测数据吻合较好;在对汕头港6个污染源的氮、磷营养盐的总量控制中,应重点关注榕江、外沙河、梅溪和新津河各污染物的入海通量;整个汕头港海域氮污染较严重,现有监测质量已不满足规定的水质保护目标,需采取严格的削减措施;整个汕头港海域磷污染相对较轻,采取一定的减排措施可以达到规定的水质保护目标;对于无机氮,榕江至少要减排95％,外沙河减排65％,其余污染源减排90％或适当降低减排率,才能使整个汕头港海域无机氮浓度基本满足相应的水质保护目标;对于活性磷酸盐,榕江至少要减排5％,梅溪、外沙河减排25％,其余污染源减排5％,才能使整个汕头港海域活性磷酸盐浓度基本满足相应的水质保护目标。     

精校机电液位置伺服系统的极点配置自校正控制

常规控制方法很难对变参数系统实现精确控制。建立精校机电液位置伺服系统离散数学模型,研究其常规控制方法。将极点配置自校正控制理论应用于精校机电液位置伺服系统中,提出该系统的极点配置自校正控制方法。仿真结果表明,系统的控制精度和跟踪性能将得以提高。     

鲜食糯玉米“雪玉糯6号”冬种高效栽培技术

仙游县于2009年和2010年冬季采取省工节本高效的栽培技术试种、示范雪玉糯6号,该品种从出苗至采青日数84．5d左右,表现生育期适中、产量高、品质较好、市场前景好、效益高等特点。该文介绍雪玉糯6号的特征特性及高产栽培技术。     

天门冬组织培养条件的优化

以天门冬(Asparagus cochinchinensis)幼嫩茎段为外植体,考察1 g/L HgCl2灭菌时间对外植体的消毒效果,以及激素浓度组合对外植体愈伤组织、不定芽的诱导和不定芽生根的影响.结果表明,1 g/L HgCl2处理7 min,外植体褐化率和污染率较低;MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.2 mg/L KT培养基中的愈伤组织诱导率最高,且生长势最强,MS+1.0 mg/L 6-BA+0.2 mg/L NAA+0.2 mg/L KT培养基中的出芽率最高;1/2MS+0.5 mg/L NAA+0.5 mg/L IBA培养基中生根率最高,可达76％.     

实用新型收割机切割机构的运动分析与仿真

针对传统切割器传动装置采用齿轮机构和平面曲柄摇杆机构相结合方式的缺点,提出了采用实用空间连杆机构,对机构进行了三维建模和运动分析,得到了输出的位移、速度、加速度运动方程,并通过仿真得到了运动曲线.仿真结果显示,该机构可以实现割刀的平稳输出,为以后的机构尺寸综合打下了基础.     

栽培基质对烟草育苗温室网纹甜瓜农艺性状及产质量的影响

为解决我国烟草育苗温室闲置期较长而利用率较低的问题,进行了不同栽培基质对烟草育苗温室网纹甜瓜农艺性状及其产质量的影响研究。结果表明:烟草育苗基质∶红沙=2∶1(基质M)上的甜瓜植株长势好于香菇渣∶红沙=2∶1(基质N),基质M上的甜瓜果实结瓜位显著低于基质N,植株最大叶面积、干鲜重显著大于基质N;美灵甜瓜果实中可溶性固形物、蔗糖、总糖、可溶性蛋白均显著高于美琪,但美琪果肉厚度、单果重、座果率、均价及产值均显著高于美灵。综合分析认为,当前栽培条件下,南雄烟区烟草育苗温室大棚网纹甜瓜生产中宜采用甜瓜品种美琪,栽培基质选择烟草育苗基质∶红沙=2∶1。     

台湾荔枝产业发展概况与展望

台湾荔枝栽培已有近300年的历史,曾是我国荔枝生产的主要区域,1988年栽培面积和产量达到历史之最,然后进入近20多年的调整期,栽培面积大幅度缩减,发展速度与其他特色小水果相比较为逊色,尽管如此,但其单产高、出口比例大、效益普遍较好,以及在新品种选育方面成绩突出,其产业结构已成功从数量规模效益转化为质量品质效益。探讨了台湾荔枝产业发展变化的历史,以加深对台湾荔枝产业发展进程的了解和认识,为相关研究提供借鉴。