Pathogen Analysis of Goat's Respiratory Diseases

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.     

不同叶龄蘖、穗氮肥组合对粳稻产量及氮素利用的影响

以主茎叶片数不同的粳稻品种吉粳88 (14片)、沈农265 (15片)和沈农1401 (16片)为试材,采用大田筒栽方式,在总施氮量225kghm–2及轻简施肥(基肥、蘖肥、穗肥)模式基础上,设置基蘖肥∶穗肥6∶4和8∶2两种施肥比例,并分设不同源、库叶龄期施氮组合即不同叶龄蘖、穗肥精确施氮组合。分析了不同源库期氮肥运筹模式对水稻农艺性状、产量及氮素利用特性的影响。结果表明:(1)在有效穗数、分化颖花数、产量和氮素利用率方面,吉粳88、沈农265、沈农1401不同氮肥运筹下最佳蘖、穗肥叶龄组合均为6∶4显著高于8∶2。(2)不同氮肥运筹下,吉粳88在8叶(叶龄指数57.1%)、沈农265在9叶(叶龄指数60.0%)、沈农1401在10叶(叶龄指数62.5%)时,即叶龄指数在60%左右时,施用蘖肥效果最佳,最终穗数最多,对保蘖起主要作用;吉粳88在11叶(叶龄指数78.6%)、沈农265在12叶(叶龄指数80.0%)、沈农1401在13叶(叶龄指数81.3%)时,即叶龄指数在80%左右时,施用穗肥效果最佳,最终穗粒数最多,对促花起主要作用。(3)吉粳88-6∶4 (8, 11),沈农265-6∶4 (9, 12),沈农1401-6∶4 (10, 13) 3组处理,在产量、氮素积累量、氮素吸收利用率、农学利用率及偏生产力等方面,显著高于同品种不同叶龄蘖、穗氮肥组合中的其他处理。因此,适当延迟蘖肥施用叶龄期(叶龄指数60%左右)、提前穗肥施用叶龄期(叶龄指数80%左右)同时增加穗肥施用比例,既可以显著提高氮素积累量、氮素吸收利用率、农学利用率及偏生产力,又能显著促进成穗率的提高和颖花数的分化,达到保蘖促花的双重作用,实现优源、扩库、充实的目标,从而获得高产。     

质量控制图在水产饲料氨基酸分析中的应用

通过对实验室自制标准物料-豆粕样品中氨基酸含量的多次测定,建立X-S质量控制图,开展水产饲料氨基酸分析日常质量控制。试验按照GB/T 18246-2000,通过微波水解-离子交换-柱后衍生-紫外检测法对豆粕样品氨基酸含量进行连续测定,并以FAPAS奶粉标准物质验证,利用偏度-峰度检验法对离群值进行判断,建立赖氨酸、甘氨酸、氨基酸总量质量控制图。结果显示,质量控制图中豆粕质控样赖氨酸、甘氨酸、氨基酸总量测定的RSD值均小于4%,FAPAS奶粉标准物质测定值落在参考范围内,建立的质量控制图能对日常氨基酸检测结果进行有效监控。结果表明,所建立氨基酸质量控制图直观有效,能对过程存在的异常因素起到预警作用,并可节约成本。     

Sequence Analysis and Prokaryotic Expression of NSP1 Gene of Bovine Rotavirus UK Strain

Rotavirus is a major cause of acute diarrhea in both many kinds of young animals and children under 5 years old.Rotavirus NSP1, a 55 ku RNA binding protein, is the product of gene 5, which can subvert innate immune responses and be one of virulent determinant factors.According to the sequence in GenBank, specific primers targeting to NSP1 gene were designed and the gene was amplified by RT-PCR, following by being cloned into the pET-28a(+) vector.It showed that the full length of NSP1 gene was 1 473 bp, encoding 491 amino acids.The NSP1 shared the highest identity with WC3 strain.The recombinant protein was induced in E.coli Rosetta(DE3) by IPTG and was analyzed by SDS-PAGE and Western blotting.The results revealed that NSP1 recombinant protein existed in the form of inclusion body with the molecular weight of 55 ku.The purified recombinant protein could be recognized by His-tag antibody.This study laid the foundation for further research on the relationship between the intracytoplasmic location of NSP1 protein and its activity.     

苗盘烟雾消毒方法对烤烟苗盘处理效果研究

为了验证苗盘烟雾消毒方法对烤烟苗盘的处理效果和安全性,试验设计了常规高锰酸钾消毒和苗盘烟雾消毒2种方法,结果表明:苗盘烟雾消毒方法省工省时,药剂用量和耗水量少,对烤烟苗盘有着较好的消毒效果,且对烟苗生长没有负面作用。     

马铃薯品种底西瑞(Desiree)悬浮细胞系的建立和培养条件的优化

悬浮细胞培养是植物生物技术中最有用的研究手段之一.本实验对马铃薯品种底西瑞( Desiree)悬浮细胞系建立的方法和优化条件进行了研究.结果表明:马铃薯品种底西瑞( Desiree)细胞生长曲线呈典型的S型,生长周期为2周左右.添加2mg/L2,4-D+1 mg/L NAA的MS培养基最适合底西瑞(Desiree)悬浮...     

插条粗度和萌芽度对降香黄檀扦插成活率的影响

在江门市林科所苗圃选择不同粗细度、不同萌芽度的降香黄檀插条进行扦插繁殖试验,每10 d进行1次成活率调查。结果表明:插条的萌芽度和粗细度均极显著影响扦插的成活率,萌芽度和粗细度的交互作用对扦插的成活率也有极显著影响;未萌芽插条扦插成活率明极显高于已萌芽插条的扦插成活率;3种粗细度的枝条中以粗细度为0.7~1.2 cm的插条扦插成活率最高;在枝条萌芽度及粗细度交互作用下,粗细度为0.7~1.2 cm的未萌芽插条扦插成活率最高,粗细度小于0.7 cm的已萌芽插条扦插成活率最低。