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61.
Jie Liu Ryo Ando Kuniyoshi Shimizu Koh Hashida Rei Makino Seiji Ohara Ryuichiro Kondo 《Journal of Wood Science》2008,54(1):68-75
In humans, steroid 5α-reductase is involved in the development of benign prostatic hyperplasia (BPH). We tested the steroid
5α-reductase inhibitory activity of the 70% acetone extract of woody plants. The tannin polymer prepared from the 70% acetone
extract showed the highest steroid 5α-reductase inhibitory activity. Furthermore, the steroid 5α-reductase inhibitory activity
of tannins was not affected by the addition of superoxide dismutase or catalase. It was concluded that the steroid 5α-reductase
inhibitory activity of condensed tannin was caused by binding to the steroid 5α-reductase, rather than from the peroxide/superoxide
produced by tannins. The tannins, prepared from tree barks, with potential for steroid 5α-reductase inhibitory activity might
be advantageous in therapy for steroid 5α-reductase diseases such as BPH or prostate cancer.
Part of this report was presented at the 54th Annual Meeting of the Japan Wood Research Society, Sapporo, August 2004 相似文献
62.
SIQIN Mari NAKAI Tomohiro HAGI Shinichi KATO Ali Mohammed PITIA Mai KOTANI Yuki ODANAKA Yasushi SUGAWARA Koh‐Ichi HAMANO Keiichiro YOGO Yoshio NAGURA Masaru FUJITA Hiroshi SASADA Eimei SATO Tetsuya KOHSAKA 《Animal Science Journal》2010,81(6):681-686
Relaxin‐like factor (RLF), also known as insulin‐like factor 3 (INSL3), is produced by testicular Leydig cells, but its specific receptor LGR8 (leucine‐rich repeat family of G‐protein‐coupled receptor 8) has not been identified in goats. This study aimed to identify complementary DNA (cDNA) sequences of goat LGR8, and characterize the expression of both RLF and LGR8 in goat testes by RT‐PCR and immunohistochemistry. Testes were collected from immature (3‐month‐old) and mature (24‐month‐old) Saanen goats, and partial cDNA sequences of the goat homologue of human LGR8 were identified. The sequence encoded a reduced peptide sequence of 167 amino acids, which corresponded to transmembrane regions 2 through 5, followed by the beginning of intracellular loop 3 of human LGR8. Expression of both LGR8 and RLF genes was drastically increased in mature testes compared with immature ones. Although RLF protein was restricted to Leydig cells, LGR8 protein was detected in both Leydig cells and seminiferous epithelial cells (possibly germ cells and Sertoli cells). These results reveal a possible existence of the RLF‐LGR8 ligand‐receptor system within the goat testis, suggesting that RLF may play a role in testicular function through LGR8 on Leydig cells and seminiferous epithelial cells in an autocrine and/or paracrine manner. 相似文献
63.
64.
The synthesis of a series of phthalimide based azo disperse dyes and their spectral properties were investigated. The azo
dyes containing phthalimide and N-methyl phthalimide structure in diazo component were synthesized in order to compare their spectral properties. The synthesized
dyes developed the color of yellow to violet and the N-substitution of the phthalimide gave a bathochromic effect on the color change. Most of the synthesized dyes exhibit negative
solvatochromism so that the absorption band of dyes moves toward shorter wavelengths as the polarity of the solvent increases.
In the case of halochromic effect, the bathochromic shift decreased steadily with the general electron donating capacity of
the substituents in the coupling component, and became negative especially when more powerful electron donating groups are
present in the coupling components ring. 相似文献
65.
Woo Sub Shim Jae Pil Kim Jung Jin Lee Joonseok Koh Ik Soo Kim 《Fibers and Polymers》2008,9(6):691-697
Novel regenerated cellulose material which was prepared from cellulose acetate fiber through the hydrolysis of acetyl groups
have been developed by an environmentally friendly process without emitting toxic substances in addition to be at low production
cost. They have composite crystalline structure constituted of cellulose II and cellulose IV. Also, they show a lamellar morphology
with an increased amorphous region, as compared to conventional regenerated cellulose such as viscose rayon and cupra rayon.
Our data obtained by several independent methods demonstrated that the adsorption properties of cellulose fibers depend predominantly
on the amorphous region. 相似文献
66.
67.
Jeong JH Kim GY Yoon SS Park SJ Kim YJ Sung CM Jang OJ Shin SS Koh HB Lee BJ Lee CY Kang MI Kim HJ Park NY Cho KO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(2):187-189
Although winter dysentery (WD) has been suspected to occur frequently in Korea, to date the exact epidemiology of WD has remained unknown. Therefore, we investigated the causative agents of WD by using electron microscopy, ELISA, RT-PCR, and nested PCR from 97 fecal samples of 32 WD-affected herds collected from 8 provinces during 2002-2004. The bovine coronavirus (BoCV) was consistently detected in all herds with WD. Of other pathogens, only coccidian oocyts were inconsistently but concurrently detected with BoCV. Ten isolates were identified as BoCV by immune electron microscope, immunofluorescent test and ELISA with antiserum to BoCV, and RT-PCR. From these results, it is concluded that WD caused by BoCV occurred in relatively high frequency and was widespread in Korea. The results provide important epidemiological data for the control and establishment of a surveillance system for WD in Korea. 相似文献
68.
Koh PO Kang BI Kim GS Oh YS Won CK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1047-1050
In this study, we have examined the possible existence of astrocyte regional heterogeneity in thrombin effect on astrocyte stellation. Neonatal astrocytes were cultured for 2 weeks from six different regions of the neonatal rat brain, including the cerebral cortex, hippocampus, brainstem, midbrain, hypothalamus and cerebellum. Culture medium was changed to DMEM containing 8-CPT-cyclic AMP (cAMP) or isoproterenol plus various concentrations of thrombin for 2 hr. Thrombin effectively blocked both cAMP- and isoproterenol-induced cell stellation in a dose-dependent manner in all regional astrocytes except cerebellar astrocytes. RT-PCR analysis showed that thrombin receptor mRNA was expressed in all regional astrocytes, suggesting that cerebellar astrocytes may maintain a unique signaling pathway downstream of the thrombin receptor. 相似文献
69.
Lee HY Naha N Ullah N Jin GZ Kong IK Koh PO Seong HH Kim MO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):791-797
Tyrosine hydroxylase (TH) is the rate-limiting enzyme of dopamine (DA) biosynthesis, which is up-regulated by vitamin C administration. Nurr1 gene is highly expressed in brain and important for midbrain DAergic cell development and survival. But, the role of vitamin C and/or vitamin E during Nurr1 expression is yet to be known. Further, the synergistic effect of vitamins C and E on TH expression has not yet been explored clinically. Therefore, we studied the effects of single or co- administration of vitamin C (0.5 mM) and vitamin E (1 mM) for 72 hr, on both TH and Nurr1 expression in in vitro primary cultured gestational days (GD) 13.5 rat ventral mesencephalon (VM) by Western blot and immunocytochemistry. Our study revealed highest expressions of both TH and Nurr1 in the vitamin C + vitamin E treated group. TH expression was also increased in the vitamin C treated group than that of the control group, but the vitamin E treated group did not show any statistically significant effect. However, both the vitamin C and the vitamin E treated groups revealed increased expression of Nurr1 protein as compared with the control group. The present experimental data suggest that vitamin C can up-regulate the protein expression of TH, but Nurr1 level was elevated either by vitamin C or by vitamin E administration. Further, vitamin E acts synergistically with vitamin C to elevate TH and Nurr1 expression, which is the most novel finding of our study and for the first time; we reported this result, since there have been no published data on the synergistic effect of both the antioxidant vitamins on TH and Nurr1 expression in VM. As the motor function defect due to the progressive loss of DAergic neuron is the major reason of Parkinsonism, therefore, the results of our study finally suggest the effective role of vitamin C and vitamin E during early treatment of Parkinsonism. 相似文献
70.
T. H. Koh L.‐H. Sng S. M. Yuen C. K. Thomas P. L. Tan S. H. Tan N. S. Wong 《Zoonoses and public health》2009,56(4):206-208
We describe three zoonotic streptococcal soft tissue infections resulting from fresh seafood contact. One was a localized thumb infection with Streptococcus iniae in an immunocompetent healthy young male resulting from a puncture wound from a crab pincer. The other two were cases of ascending upper limb cellulitis associated with bacteraemia in mastectomy patients. One of these infections was caused by S. iniae while the other was caused by Streptococcus dysgalactiae subsp. dysgalactiae, a species that has not been previously described as a cause of zoonotic infection. Hence when cleaning raw seafood, protective equipment should be used to minimize the risk of percutaneous injuries. 相似文献