全文获取类型
收费全文 | 12689篇 |
免费 | 362篇 |
国内免费 | 19篇 |
专业分类
林业 | 986篇 |
农学 | 476篇 |
基础科学 | 58篇 |
1909篇 | |
综合类 | 938篇 |
农作物 | 1068篇 |
水产渔业 | 877篇 |
畜牧兽医 | 5658篇 |
园艺 | 271篇 |
植物保护 | 829篇 |
出版年
2022年 | 101篇 |
2021年 | 121篇 |
2020年 | 102篇 |
2019年 | 116篇 |
2018年 | 252篇 |
2017年 | 342篇 |
2016年 | 347篇 |
2015年 | 396篇 |
2014年 | 398篇 |
2013年 | 438篇 |
2012年 | 791篇 |
2011年 | 718篇 |
2010年 | 330篇 |
2009年 | 291篇 |
2008年 | 376篇 |
2007年 | 339篇 |
2006年 | 309篇 |
2005年 | 1015篇 |
2004年 | 865篇 |
2003年 | 627篇 |
2002年 | 310篇 |
2001年 | 275篇 |
2000年 | 151篇 |
1999年 | 222篇 |
1992年 | 145篇 |
1991年 | 172篇 |
1990年 | 201篇 |
1989年 | 184篇 |
1988年 | 202篇 |
1987年 | 153篇 |
1986年 | 161篇 |
1985年 | 149篇 |
1984年 | 114篇 |
1983年 | 108篇 |
1982年 | 43篇 |
1979年 | 147篇 |
1978年 | 117篇 |
1977年 | 110篇 |
1976年 | 105篇 |
1975年 | 151篇 |
1974年 | 148篇 |
1973年 | 138篇 |
1972年 | 160篇 |
1971年 | 133篇 |
1970年 | 101篇 |
1969年 | 113篇 |
1968年 | 115篇 |
1967年 | 130篇 |
1966年 | 108篇 |
1965年 | 74篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
72.
73.
Recent studies have shown that tumour cells express tumour necrosis factor-inducible gene 6 (TSG-6) and its protein, which is known to play a key role in regulating excessive immune responses and proliferation and growth of mesenchymal stem cells (MSCs). It has not been confirmed whether the inhibition of TSG-6 for tumour cells can suppress tumour cell growth and regulate the activation of immune cells in the tumour microenvironment (TME). TSG-6-specific small interfering RNA was transfected into canine and human breast cancer cells (CIPp, CIPm and BT-20). TSG-6-down-regulated (siTSG-6) cells showed decreased cell proliferation, migration, and invasion abilities. Decreased mRNA expressions of NF-κB, STAT3 and Sox2, confirming that TSG-6 is an upper factor governing tumour growth and metastasis. Notably, siTSG-6 cells showed significantly decreased expression levels of CD44 and PD-L1. Direct and indirect co-culture of canine peripheral blood mononuclear cells (cPBMCs) and the siTSG-6 cells showed significant activation in M1 type macrophages and cytotoxic T cells. They also showed a tendency to decrease in the expression of CTLA-4 and increase in the expression of PD-1. In conclusion, this study suggests that the down-regulation of TSG-6 in breast cancer cells could not only suppress tumour growth and metastasis, and but also regulate TME. Since modulation of immune checkpoint proteins occurs in both tumour cells and immune cells, inhibiting TSG-6 and its protein within the TME could be novel therapeutic target for anticancer treatment. 相似文献
74.
W. Wallender J. Rhoades M. Weinberg S. Lee C. Uptain D. Purkey 《Irrigation and Drainage Systems》2002,16(4):311-326
Land retirement is ceasing irrigation withthe goal of reducing load, in general, ofdissolved constituents and, in particular,of trace elements, present in subsurfacedrainage generated from irrigated lands. Retirement is achieved through a process ofgoal setting, strategy development anddetermining effects, developing landselection criteria, implementation, andmonitoring. In this study, effects of landretirement are evaluated using hydrologic,soil and economic models as well as resultsfrom a field demonstration study. From themodeling and field monitoring, a process isdeveloped to meet the goals of a landretirement program in the San JoaquinValley of California.Potential negative effects listed for landretirement included loss of agriculturalproductivity, perhaps permanently, and lossof revenue to surrounding communities. Uncertainties included those associatedwith reuse of retired lands as wildlifehabitat, with retired-land maintenanceincluding dust control, with potentialpreservation of retired lands in reservefor future re-introduction to irrigated ordry-land agriculture, and withinstitutional changes concerning repaymentof federal and state water contracts. Benefits would accrue from economic returnto the landowner from the sale of property,the sale or lease of irrigation watersupply, the reduced cost of handlingdrainage, and allocation of freed-up waterto beneficial uses, and the reduced risk ofselenium exposure to fish and wildlife.A recommended sequential approach to selectand manage retired land is to identifyprimary objectives; formulate and implementarea-specific land retirement scenarios;measure biologic, hydrologic, soils andeconomic consequences in the short term andthe long term and manage and monitorretired lands based on dynamic biologic,hydrologic and soil conditions. 相似文献
75.
Comparison of water status indicators for young peach trees 总被引:12,自引:1,他引:12
We measured a series of physiological and physical indicators and compared them to xylem sap flow, to identify the most sensitive and reliable plant water status indicator. In the growing season of 1998, 4-year-old peach trees (Prunus persica Batsch cv. 'Suncrest', grafted on 'GF 677' rootstock) were studied under two irrigation treatments, 25 l dayу and no irrigation, and during recovery. Trials were conducted near Pisa (Italy) in a peach orchard situated on a medium clay loam soil and equipped with a drip-irrigation system (four 4 l hу drippers per tree). Measurements of leaf water potential (W), stem water potential (S), and leaf temperature (Tl) were taken over 5 days (from dawn to sunset) and analyzed in conjunction with climatic data, sap flow (SF), trunk diameter fluctuation (TDF) and soil water content (SWC). Physiological indicators showed substantial differences in sensitivity. The first indication of changes in water status was the decrease of stem radial growth. TDF and SF revealed significant differences between the two irrigation treatments even in the absence of differences in pre-dawn leaf water potential (pdW), up until now widely accepted as the benchmark of water status indicators. Irrigated trees showed a typical trend in SF rate during the day, while in non-irrigated plants the maximum peak of transpiration was anticipated. Measurements of water potential showed S to be a better indicator of tree water status than W. Tl was found to have poor sensitivity. In conclusion, we found the sensitivity of the indicators from the most to the least was: TDF >SF rate >SF cumulated = pdW=S>mdW>Tl. 相似文献
76.
Yaohu?KangEmail author Feng-Xin?Wang Hai-Jun?Liu Bao-Zhong?Yuan 《Irrigation Science》2004,23(3):133-143
A field experiment comparing different irrigation frequencies and soil matric potential thresholds on potato evapotranspiration (ET), yield (Y) and water-use efficiency (WUE) was carried out in a loam soil. The experiment included five treatments for soil matric potential: F1 (-15 kPa), F2 (-25 kPa), F3 (-35 kPa), F4 (-45 kPa) and F5 (-55 kPa) and six treatments for irrigation frequency: N1 (once every day), N2 (once every 2 days), N3 (once every 3 days), N4 (once every 4 days), N6 (once every 6 days) and N8 (once every 8 days). Results indicate that both soil matric potential and drip irrigation frequency influenced potato ET, Y and WUE. Potato ET increased as irrigation frequency and soil matric potential increased. Comparing soil water potential, the highest ET was 63.4 mm (32.1%) more than the lowest value. Based on irrigation frequency treatments, the highest ET was 36.7 mm (19.2%) more than the lowest value. Potato Y and WUE were also found to increase as irrigation frequency increased. Potato Y increased with an increase in soil water potential then started to decrease. The highest Y and WUE values were achieved with a soil matric potential threshold of -25 kPa and an irrigation frequency of once a day.Communicated by J. Ayars 相似文献
77.
Enteropathogenic Escherichia coli (EPEC) is a major cause of infantile diarrhea in developing countries. However, sporadic outbreaks caused by this microorganism in developed countries are frequently reported recently. As an important zoonotic pathogen, EPEC is being monitored annually in several countries. Hallmark of EPEC infection is formation of attaching and effacing (A/E) lesions on the small intestine. To establish A/E lesions during a gastrointestinal tract (GIT) infeciton, EPEC must thrive in diverse GIT environments. A variety of stress responses by EPEC have been reported. These responses play significant roles in helping E. coli pass through GIT environments and establishing E. coli infection. Stringent response is one of those responses. It is mediated by guanosine tetraphosphate. Interestingly, previous studies have demonstrated that stringent response is a universal virulence regulatory mechanism present in many bacterial pathogens including EPEC. However, biological signficance of a bacterial stringent response in both EPEC and its interaction with the host during a GIT infection is unclear. It needs to be elucidated to broaden our insight to EPEC pathogenesis. In this review, diverse responses, including stringent response, of EPEC during a GIT infection are discussed to provide a new insight into EPEC pathophysiology in the GIT. 相似文献
78.
Lee KH Cho JY Lee HJ Ma YK Kwon J Park SH Lee SH Cho JA Kim WS Park KH Moon JH 《Journal of agricultural and food chemistry》2011,59(18):10124-10128
Two novel caffeoylmalic acid methyl esters, 2-O-(trans-caffeoyl)malic acid 1-methyl ester (6) and 2-O-(trans-caffeoyl)malic acid 4-methyl ester (7), were isolated from pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) fruit peels. In addition, 5 known hydroxycinnamoylmalic acids and their methyl esters were identified: 2-O-(trans-coumaroyl)malic acid (1), 2-O-(cis-coumaroyl)malic acid (2), 2-O-(cis-coumaroyl)malic acid 1-methyl ester (3), 2-O-(trans-coumaroyl)malic acid 1-methyl ester (4), and 2-O-(trans-caffeoyl)malic acid (phaselic acid, 5). The chemical structures of these compounds were determined by spectroscopic data from ESI MS and NMR. Of all the isolated compounds, five hydroxycinnamoylmalic acids and their methyl esters (2-4, 6, 7) were identified in the pear for the first time. 相似文献
79.
Kim JK Lee SY Chu SM Lim SH Suh SC Lee YT Cho HS Ha SH 《Journal of agricultural and food chemistry》2010,58(24):12804-12809
Flavonoids and carotenoids of pigmented rice ( Oryza sativa L.), including five black cultivars and two red cultivars, from Korea were characterized to determine the diversity among the phytochemicals and to analyze the relationships among their contents. Black cultivars were higher in flavonoids and carotenoids than the red and white cultivars. The profiles of eight phytochemicals identified from the rice grains were subjected to principal component analysis (PCA) to evaluate the differences among cultivars. PCA could fully distinguish between these cultivars. The Heugjinjubyeo (BR-1) and Heugseolbyeo (BR-2) cultivars were separated from the others based on flavonoid and carotenoid concentrations. Flavonoid contents had a positive correlation with carotenoid contents among all rice grains. The BR-1 and BR-2 cultivars appear to be good candidates for future breeding programs because they have simultaneously high flavonoid and carotenoid contents. 相似文献
80.
Utilization of biochar impregnated with anaerobically digested slurry as slow‐release fertilizer 下载免费PDF全文
We examined the possibility of an environment‐friendly slow‐release fertilizer (SRF) made of biochar impregnated by anaerobically digested slurry. The biochar materials were produced from three types of feedstocks (orange peel, residual wood, water‐treatment sludge) at different temperatures of 300°C, 500°C, and 700°C via pyrolysis. The release behaviors of the water‐soluble K+, Ca2+, and Mg2+ were similar for all impregnated biochars and the commercial SRF used. The water‐retention capacity was greatly improved by mixing the biochar‐SRF with the soil. The yield of lettuce was lower for the biochar‐SRF applications of 3.7 to 34.2 t ha–1 than for the commercial SRF application of 51.4 t ha–1. This might be due to excessive increase of soil pH for the biochar‐SRF application. Based on these results, the authors concluded that the biochar impregnated with nutrients could become an effective slow‐release K+ fertilizer. 相似文献