全文获取类型
收费全文 | 23268篇 |
免费 | 1068篇 |
国内免费 | 2691篇 |
专业分类
林业 | 2857篇 |
农学 | 3499篇 |
基础科学 | 1898篇 |
4130篇 | |
综合类 | 6713篇 |
农作物 | 1330篇 |
水产渔业 | 879篇 |
畜牧兽医 | 3293篇 |
园艺 | 729篇 |
植物保护 | 1699篇 |
出版年
2024年 | 46篇 |
2023年 | 211篇 |
2022年 | 528篇 |
2021年 | 866篇 |
2020年 | 787篇 |
2019年 | 756篇 |
2018年 | 544篇 |
2017年 | 766篇 |
2016年 | 751篇 |
2015年 | 1009篇 |
2014年 | 924篇 |
2013年 | 1182篇 |
2012年 | 1417篇 |
2011年 | 1466篇 |
2010年 | 1441篇 |
2009年 | 1378篇 |
2008年 | 1277篇 |
2007年 | 1412篇 |
2006年 | 1305篇 |
2005年 | 1117篇 |
2004年 | 549篇 |
2003年 | 498篇 |
2002年 | 415篇 |
2001年 | 441篇 |
2000年 | 546篇 |
1999年 | 688篇 |
1998年 | 615篇 |
1997年 | 532篇 |
1996年 | 516篇 |
1995年 | 508篇 |
1994年 | 465篇 |
1993年 | 414篇 |
1992年 | 388篇 |
1991年 | 302篇 |
1990年 | 248篇 |
1989年 | 207篇 |
1988年 | 164篇 |
1987年 | 91篇 |
1986年 | 63篇 |
1985年 | 32篇 |
1984年 | 16篇 |
1983年 | 34篇 |
1982年 | 18篇 |
1981年 | 23篇 |
1980年 | 11篇 |
1979年 | 7篇 |
1974年 | 9篇 |
1965年 | 6篇 |
1964年 | 6篇 |
1963年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
根据国内蚕桑生产现状,结合重庆市蚕桑生产近期情况,围绕如何提高茧丝品质这一议题,从桑叶物质基础及品种,施肥,论述了提高茧丝品质应采取的科学技术措施. 相似文献
92.
93.
Alinovi CA Ward MP Couëtil LL Wu CC 《Journal of the American Veterinary Medical Association》2003,223(11):1640-1644
OBJECTIVES: To assess methods of detecting environmental contamination with Salmonella organisms and evaluate a cleaning and disinfection protocol for horse stalls in a veterinary teaching hospital. DESIGN: Original study. SAMPLE POPULATION: 37 horses with diarrhea likely to be caused by Salmonella infection and their stall environments. PROCEDURES: Fecal samples were collected from horses daily during hospitalization; samples were obtained from stall sites after cleaning and application of disinfectants. Fecal and environmental samples were cultured for Salmonella spp and tested via polymerase chain reaction (PCR) assay to detect Salmonella DNA. RESULTS: 1 horse died and 2 were discharged prior to sample collection. Fecal samples from 9 of 34 horses yielded growth of Salmonella organisms on bacteriologic culture, and 23 yielded positive results via PCR assay on > or = 1 occasion. Among environmental samples from 21 stalls, salmonellae were detected at > or = 1 stall site on 6 of 78 occasions, and > or = 1 stall site yielded positive results via PCR assay on 69 of 77 occasions. Salmonella DNA was detected more frequently in samples of stall drains, cracks, and corners. Salmonella spp were cultured from samples of 3 stalls after both initial and second cleaning and disinfection cycles, but no organisms were detected in samples obtained after use of a peroxygen disinfectant. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that stalls in which horses with salmonellosis were housed should only be used to accommodate newly hospitalized horses after samples (collected after 2 cycles of cleaning and disinfection) from drains, cracks, and corners yield negative results on bacteriologic culture. 相似文献
94.
几种鸡胚原代细胞的制备方法 总被引:1,自引:0,他引:1
在病毒分离、繁殖,中和试验及药物毒理试验中,细胞培养是经常被选用的技术。本文描述通过选择不同日龄的鸡胚,分别对皮肤上皮细胞、肝细胞和肾细胞进行了组织培养,确定了生长所需的培养液要求。认为12日龄鸡胚适于皮肤上皮细胞的制备,17~20日龄鸡胚适合于肝细胞、肾细胞的制备,并确定了细胞生长的pH条件。 相似文献
95.
Liang Deng Hongyun Duan Xiang Zhang Shenming Zeng Changxin Wu Guocai Han 《Journal of Equine Veterinary Science》2014
China was one of the first countries to use artificial insemination (AI) in equids, and it achieved international recognition for its widespread application of AI to commercial horse breeding. This article reviews the history of equine AI in China. The technique originated from the high demand for horses to be used in agriculture, transportation, and the military. Artificial insemination was identified as an ideal tool for Chinese horse breeding to improve the productivity of native horses, especially during 1950-1970. Presently, AI is still practiced commonly in China, and it includes the use of fresh semen and transported cooled semen. The use of frozen semen has also been resumed to broaden the range of elite Sporthorse stallions and to preserve threatened or endangered native breeds. Accurate prediction of the optimum time for insemination depended mainly on transrectal palpation of the mare's ovaries. In addition to controlled methods of insemination, factors like volume and number of spermatozoa in the inseminate and timing and frequency of insemination were optimized to accomplish high fertility rates in the field. Production of hinnies and mules for agricultural labor and donkeys for meat and hide gelatin stimulated a nationwide upsurge of research into the reproductive physiology of and AI technology in donkeys. In the future, there will be further increases in the use of traditional and modern AI techniques in the breeding of Chinese equids. 相似文献
96.
近段时间CBOT玉米、小麦价格出现明显上涨,CBOT小麦价格已经突破前期盘整区创出10年来的新高;CBOT玉米也突破前期260美分/蒲式耳一线的压力,期价将进入新的价格区间,后市将如何发展以及如何应对某些变化?笔者将作一简单分析。1国际、国内玉米走势分析1.1国际投资资金中长期介入 相似文献
97.
98.
99.
通过两个试验,研究在体外模拟瘤胃发酵条件下N-乙酰-DL-蛋氨酸的降解率。试验1:N-乙酰-DL-蛋氨酸在pH值6.5、温度为39℃的缓冲液中孵育9h,其降解率为3.3%。试验2:运用瘤胃体外发酵技术研究N-乙酰-DL-蛋氨酸不同添加量对pH值、氨态氮、微生物蛋白动态变化的影响。结果表明,添加0.4、0.6gN-乙酰-DL-蛋氨酸发酵3~6h,均能够显著降低体系的pH值;添加0.6gN-乙酰-DL-蛋氨酸发酵12h能够显著提高氨态氮的浓度;N-乙酰-DL-蛋氨酸在发酵过程中不影响微生物蛋白产量。以上结果显示,N-乙酰-DL-蛋氨酸在体外模拟瘤胃发酵的条件下可以稳定存在。 相似文献
100.