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101.
Jing Zhang Bishow Poudel Kevin Kenworthy Joseph Bryan Unruh Diane Rowland John E. Erickson Jason Kruse 《Journal of Agronomy and Crop Science》2019,205(1):1-12
Water shortages have become more chronic as periodic droughts prolong and water demand for urban and agricultural use increases. Plant drought responses involve coordinated mechanisms in both above‐ and below‐ground systems, yet most studies lack comparisons of root and canopy responses under water scarcity and recovery. This is particularly true of research focused on warm‐season turfgrasses in sandy soils with extremely low water holding capacity. To address the lack of examination of coordinated stress and recovery responses, this study compared the above‐ and below‐ground plant responses during a dry‐down period of 21 days and recovery among four warm‐season turfgrass species in the field. Canopy drought responses and recovery were quantified using digital image analysis. In situ root images were captured using a minirhizotron camera system. Common bermudagrass [Cynodon dactylon (L.) Pers.] endured the entire drought period without losing 50% green cover while other species lost 50% green cover in 11–34 days predicted from the regression. The interspecific differences in drought resistance were mainly due to root characteristics. Other drought mechanisms appear to be responsible for differences identified in drought resistance between “Zeon” and “Taccoa Green” manilagrass [Zoysia matrella (L.) Merr.]. Recovery was delayed for up to 2 weeks in the second year, warranting further evaluation for turfgrass persistence under long‐term drought. Three‐week drought posed no threat to the survival of zoysiagrass. Species and genotypic variations were found in achieving full post‐recovery, which can be used to develop water conservation strategies and to adjust consumer expectations. 相似文献
102.
Predicting the potential effects of changes in climate on freshwater species requires an understanding of the relationships between physiological traits and environmental conditions among populations. While water temperature is a primary factor regulating metabolic rates in freshwater ectotherms, how metabolic rates vary across the species range is unclear. In addition, photoperiod has also been hypothesised to influence metabolic rates in freshwater taxa based on seasonal changes in activity rates. Using an experimental approach, we investigated whether variation in routine metabolic rate (RMR) and sensitivity of RMR to changes in temperature are correlated with local thermal regimes, photoperiods and body mass among ten populations across the geographic range of the Bluntnose minnow (Pimephales notatus), a North American freshwater fish species. Routine metabolic rate data were collected from populations acclimatised to three temperature treatments (9, 18 and 27°C) and correlated with water temperature and photoperiod estimates at collection locations for each population. Routine metabolic rate was negatively correlated with minimum photoperiod at 9°C, negatively correlated with weekly high temperature at 18°C and positively correlated with weekly high temperature at 27°C. Body mass was also a predictor of RMR at each temperature treatment. Thermal sensitivity of RMR was positively correlated with weekly high temperature, indicating that individuals from warmer low latitude populations experienced greater sensitivity of RMR to changes in temperature than individuals from cooler high latitude populations. These results indicate differential responses among populations to variation in temperature and suggest the importance of recognising this variation when characterising responses of freshwater taxa to increases in water temperature. 相似文献
103.
Antognoli MC Garry FB Hirst HL Lombard JE Dennis MM Gould DH Salman MD 《Veterinary microbiology》2008,127(3-4):300-308
Mycobacterium avium subspecies paratuberculosis (MAP) disseminated infection in dairy cattle affects animal health and productivity and is also a potential public health concern. The study objectives were to characterize MAP disseminated infection in dairy cattle and to determine the role of antemortem tests in detecting cattle with disseminated infection. Forty culled dairy cows representing a variety of serum enzyme-linked immunosorbent assay (ELISA) results and body conditions were selected for the study. The physical condition of the cows was assessed via clinical examination prior to euthanasia and blood and feces were collected and tested by serum ELISA and fecal culture, respectively. Fifteen tissues were aseptically collected from each cow during necropsy and cultured for isolation of MAP. Disseminated infection was diagnosed when MAP was isolated in tissues other than the intestines or their associated lymph nodes (LNs) and was distinguished from infection found only in the gastrointestinal tissues and from absence of infection. Of the 40 cows in the study, 21 had MAP disseminated infection. Results showed that 57% (12/21) of cows with disseminated infection had average to heavy body condition and no diarrhea. Cows with disseminated infection had no to minimal gross pathologic evidence of infection in 37% (8/21) of cases. Only 76% (16/21) of cows with disseminated infection had positive historical ELISA results and only 62% (13/21) had a positive ELISA at slaughter. Thus, antemortem evidence of MAP infection was lacking in a high proportion of cows where MAP disseminated infection was confirmed. 相似文献
104.
Moulana M Evenhuis J Albertino M Godwin U Kountikov EI Stuge TB Wilson M Bengtén E Miller NW McConnell TJ 《Veterinary immunology and immunopathology》2008,126(1-2):120-130
This study characterizes four monoclonal antibodies (mAb) developed against the major histocompatibility complex (MHC) class II beta chain of the channel catfish, Ictalurus punctatus. Immunoprecipitations using catfish clonal B cells revealed that each of these mAbs immunoselected proteins of approximately 32 and 36 kD, which are of the appropriate sizes for MHC class II alpha and beta chains, respectively. Cell distribution studies using a fluorescence-activated cell sorter (FACS) combined with RT-PCR analyses demonstrated that MHC class II beta is expressed at a high density on catfish clonal macrophage, B and T cell lines, on alloantigen stimulated leukocytes, and on lipopolysaccharide-induced B-cell blasts. Collectively, these results demonstrate the potential importance of these antibodies as reagents in future studies dealing with the functional role of MHC class II molecules in immune recognition of self from non-self. 相似文献
105.
Gallbladder mucoceles are potentially fatal in dogs. Multiphase CT angiography was performed to evaluate the canine gallbladder in three conditions: no sludge, sludge occupying ≥25% of the lumen, and mucoceles. Twenty dogs with normal hepatobiliary bloodwork and no‐to‐minimal gallbladder sludge, 13 dogs with normal bloodwork and ≥25% sludge in the gallbladder lumen, and 18 dogs with histologically confirmed gallbladder mucoceles were enrolled in a prospective, observational diagnostic accuracy study. Three regions of interest (ROI) were stratified in the dorsal‐ventral orientation and a single ROI was measured within the hepatic parenchyma. Mean attenuation and presence of mineral were recorded. Average Hounsfield units (HU) were recorded for precontrast, arterial, portovenous, and late venous phases. The overall median HU value for mucoceles was significantly higher than gallbladders without sludge and with sludge; precontrast median overall attenuation was 49.3, 35.8, and 39.7 HU, respectively (P < .000004). Mineral was seen in four (20%) dogs with no sludge, seven (56%) dogs with sludge, and nine (50%) dogs with mucoceles. Mineral in the dogs with mucoceles was located within the central aspect of the gallbladder lumen in 67% of mucoceles; this mineral distribution was not seen in any dog without a mucocele. Computed tomography can differentiate a subset of gallbladder mucoceles from dogs with and without gallbladder sludge, especially in the precontrast series. An HU value of 48.6 is 52% sensitive and 96% specific for a gallbladder mucocele. A hyperattenuating gallbladder on precontrast CT images and centrally distributed mineral can be a gallbladder mucocele. 相似文献
106.
Katherine C. Kral-OBrien Ryan F. Limb Torre J. Hovick Jason P. Harmon 《Strength and Conditioning Journal》2019,72(2):301-309
Rangeland management strategies impact biodiversity, the quality and quantity of ecosystem services, and overall rangeland resiliency. Previous management strategies, coupled with climate change, have led to widespread invasion by Kentucky bluegrass (Poa pratensis; bluegrass) in the Northern Great Plains, United States. Bluegrass invasions are expected to have detrimental impacts on biodiversity conservation and ecosystem services provided by rangelands. Yet none have investigated how bluegrass invasions influence pollinator populations, which are a prominent conservation concern and provide ecosystem services. We measured the impact of bluegrass invasion on mixed-grass prairie forb and butterfly communities. Obligate grassland butterflies, those that rely on grasslands, decreased as bluegrass cover increased, including the threatened Hesperia dacotae. Conversely, the abundance of facultative grassland butterflies, those found in grasslands but not fully dependent on them for their life history, increased as bluegrass increased. Moreover, plant species diversity and flowering forb species richness decreased as bluegrass cover increased. Overall, bluegrass invasion led to butterfly and plant community simplification, signaling a loss of biodiversity and potentially ecosystem services. Our research is the first to quantify how grassland butterflies and the floral resources they depend on are negatively impacted by bluegrass invasion. Resource managers should adopt management strategies that reduce bluegrass cover and improve nectar and host resources for obligate grassland butterflies. Management choices that removed disturbance regimes inherent to the Northern Great Plains (i.e., fire and grazing) led to bluegrass dominance in the region. Therefore, restoring disturbance regimes may be one way to reduce bluegrass and benefit pollinator populations. 相似文献
107.
108.
Rory A. OShaughnessy Gregory G. Habing Wondwossen A. Gebreyes Andrew S. Bowman J. Scott Weese Joyce Rousseau Jason W. Stull 《Zoonoses and public health》2019,66(7):861-870
Swine are known reservoirs for Clostridioides difficile, formerly known as Clostridium difficile, and transmission from swine to human farm workers is strongly suggested by previous studies. This cross‐sectional study evaluated the potential role of farm environmental surfaces, including those in worker breakrooms and swine housing areas, in the possible transmission of C. difficile from swine to farm workers. Environmental surfaces and piglet faeces at 13 Ohio swine farms were sampled in 2015. Typical culturing techniques were performed to isolate C. difficile from samples, and amplification of toxin genes (tcdA, tcdB and cdtB) and PCR‐ribotyping were used to genetically characterize recovered isolates. In addition, sequencing of toxin regulatory gene, tcdC, was done to identify the length of identified deletions in some isolates. A survey collected farm‐level management risk factor information. Clostridioides difficile was recovered from all farms, with 42% (188/445) of samples testing positive for C. difficile. Samples collected from all on‐farm locations recovered C. difficile, including farrowing rooms (60%, 107/178), breakrooms (50%, 69/138) and nursery rooms (9%, 12/129). Three ribotypes recovered from both swine and human environments (078, 412 and 005) have been previously implicated in human disease. Samples taken from farrowing rooms and breakrooms were found to have greater odds of C. difficile recovery than those taken from nursery rooms (OR = 40.5, OR = 35.6, p < .001 respectively). Farms that weaned ≥23,500 pigs per year had lower odds of C. difficile recovery as compared to farms that weaned fewer pigs (OR = 0.4, p = .01) and weekly or more frequent cleaning of breakroom counters was associated with higher odds of C. difficile recovery (OR = 11.7, p < .001). This study provides important insights into the presence and characterization of C. difficile found in human environments on swine farms and highlights how these areas may be involved in transmission of C. difficile to swine farm workers and throughout the facility. 相似文献
109.
Amandine Gasc Benjamin L. Gottesman Dante Francomano Jinha Jung Mark Durham Jason Mateljak Bryan C. Pijanowski 《Landscape Ecology》2018,33(8):1399-1415
Context
While remote sensing imagery is effective for quantifying land cover changes across large areas, its utility for directly assessing the response of animals to disturbance is limited. Soundscapes approaches—the recording and analysis of sounds in a landscape—could address this shortcoming.Objectives
In 2011, a massive wildfire named “the Horseshoe 2 Burn” occurred in the Chiricahua National Monument, Arizona, USA. We evaluated the impact of this wildfire on acoustic activity of animal communities.Methods
In 2013, soundscape recordings were collected over 9 months in 12 burned and 12 non-burned sites in four ecological systems. The seasonal and diel biological acoustic activity were described using the “Bioacoustic Index”, a detailed aural analysis of sound sources, and a new tool called “Sonic Timelapse Builder” (STLB).Results
Seasonal biophony phenology showed a diurnal peak in June and a nocturnal peak in October in all ecological systems. On June mornings, acoustic activity was lower at burned than at non-burned sites in three of four ecological systems, due to a decreased abundance of cicadas directly impacted by the death of trees. Aural analyses revealed that 55% of recordings from non-burned sites contained insect sounds compared to 18% from burned sites. On October nights, orthopteran activity was more prevalent at some burned sites, possibly due to post-fire emergence of herbaceous.Conclusions
Soundscape approaches can help address long-term conservation issues involving the responses of animal communities to wildfire. Acoustic methods can serve as a valuable complement to remote sensing for disturbance-based landscape management.110.