Long chain n-3 polyunsaturated fatty acid concentration and color and lipid stability of muscle from heifers offered a ruminally protected fish oil supplement

Beef heifers (n = 48) were offered, daily, a 1.5 kg ration that contained 0, 69, 138, and 275 g of ruminally protected fish oil (RPFO) fortified with vitamin E (5000 IU/kg). The fatty acid profile of neutral (NL) and polar lipids (PL) of neck muscle was analyzed by GC-FID. Minced muscle was displayed in an 80% O(2):20% CO(2) atmosphere under simulated retail display conditions. Muscle α-tocopherol concentrations did not differ. For PL, the proportions of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increased (P < 0.05, linearly and quadratically, respectively). For NL, the proportion of EPA was not affected but DHA increased linearly and quadratically (P < 0.05). Supplementation did not affect color stability of ground beef, but lipid oxidation was higher (P < 0.001) for 275 g of RPFO on day 10 of display. In conclusion, supplementation with RPFO increased muscle EPA and DHA with no effect on color stability while lipid oxidation was increased only at the highest level of supplementation, after prolonged display.     

Applicability of Different Indices to Evaluate Nutrient Status of Winter Wheat in the Organic System

Nitrogen (N), phosphorus (P), and potassium (K) nutrient status of two winter wheat cultivars (Kobra and Juma) in the organic crop production system was compared with integrated and conventional systems. This research was conducted between 1998 and 2000 at the Experimental Station in Osiny (Lublin province, Poland) on a grey-brown podzolic soil. To determine N, P, and K contents shoot samples of wheat were taken at the shooting and earing growth stages. Measurements of chlorophyll content were performed with a chlorophyll meter (SPAD) to estimate N nutrient status. Four different methods [Nitrogen Nutrient Index (NNI), Soil Plant Analysis Development (SPAD), Diagnostic Recommendation Integrated System (DRIS), and Sufficiency Range (SR)] of nutrient status evaluation were compared. Nitrogen (N) and potassium (K) nutrient status of wheat cultivated in the organic system was lower compared with the other farming systems. Assuring sufficient supply of nitrogen to cereals under the conditions of organic farming is particularly difficult in early growth stages. In the integrated and conventional systems wheat was sufficiently supplied with NPK. Kobra cv. proved to be more adapted to the organic system. Results indicate that classical nutrient status indices are of lesser use for organic farming.     

Clostridial abomasal disease in Connecticut dairy calves

Over 2 years, 24 dairy calves died of emphysematous abomasitis and abomasal bloat. Anaerobic cultures of necrotic abomasal mucosa yielded Clostridium perfringens from 10 of 15 calves. Sarcina were observed in 17 of 22 examined histologically. A change in the antibiotic regimen for newborns and improved sanitizing of feeding utensils eliminated further losses.     

Nutrition and feed management in the ostrich (Struthio camelus var. domesticus)

The ostrich is an important animal in many livestock industries and in the developing world ostrich production is a valuable source of foreign currency netted from the export of meat and skins. Its successful growth and reproductive performance is dependent upon good nutrition. In Zimbabwe, one of the greatest costs involved in the upkeep of ostrich breeder birds is purchased feed (US$4555.45/10 birds p.a.). In order to cover these costs, the producer needs to ensure an adequate throughput of birds for slaughter. The energy provided by a particular feed source is important for growth and development of the bird. More research is required in ostrich nutrition.     

Suitable transfection methods for single particle tracing in plant suspension cells

Background

Besides classical utilization of wood and paper, lignocellulosic biomass has become increasingly important with regard to biorefinery, biofuel production and novel biomaterials. For these new applications the macromolecular assembly of cell walls is of utmost importance and therefore further insights into the arrangement of the molecules on the nanolevel have to be gained. Cell wall recalcitrance against enzymatic degradation is one of the key issues, since an efficient degradation of lignocellulosic plant material is probably the most crucial step in plant conversion to energy. A limiting factor for in-depth analysis is that high resolution characterization techniques provide structural but hardly chemical information (e.g. Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM)), while chemical characterization leads to a disassembly of the cell wall components or does not reach the required nanoscale resolution (Fourier Tranform Infrared Spectroscopy (FT-IR), Raman Spectroscopy).

Results

Here we use for the first time Scanning Near-Field Optical Microscopy (SNOM in reflection mode) on secondary plant cell walls and reveal a segmented circumferential nanostructure. This pattern in the 100 nm range was found in the secondary cell walls of a softwood (spruce), a hardwood (beech) and a grass (bamboo) and is thus concluded to be consistent among various plant species. As the nanostructural pattern is not visible in classical AFM height and phase images it is proven that the contrast is not due to changes in surfaces topography, but due to differences in the molecular structure.

Conclusions

Comparative analysis of model substances of casted cellulose nanocrystals and spin coated lignin indicate, that the SNOM signal is clearly influenced by changes in lignin distribution or composition. Therefore and based on the known interaction of lignin and visible light (e.g. fluorescence and resonance effects), we assume the elucidated nanoscale structure to reflect variations in lignification within the secondary cell wall.     

Comparison of Two Methods For Determining the Discoloration Potential of Potato Tubers Based on Their Chemical and Biochemical Properties

The aim of this study was to compare the homogenization and visual methods that are used to determine the discoloration potential of potato tubers. Two German cultivars, Agria and Satina, and two Polish cultivars, Lena and Mors, were planted during 1998 and 1999 in Goettingen, Germany, and in Bydgoszcz, Poland. The discoloration potential of the tubers was measured after harvesting and after 6 months of storage at 4 C and 8 C. Both methods correlated significantly at harvest (0.709,P < 0.001). After storage the correlation was significant but low (r = 0.403,P < 0.001) probably because of physical changes in the tuber during storage. The visual method can be used as a fast method, especially at harvest. However, the homogenization and visual methods determine, to a certain degree, different tuber properties leading to the discoloration potential. Therefore, a complete comparison is not possible. The methods studied in this paper can complement but not substitute each other. Reliable comparisons between institutes require use of the same method to determine the discoloration potential of the tuber.