Sweet white Lupinus Albus (cv buttercup) as a feedstuff for layers

1. An experiment was conducted with laying hens to determine to what extent sweet white lupins (Lupinus albus, cv Buttercup) could be used as a protein source in their diets.

2. Twenty five individually caged 20 week‐old Hisex laying hens were used per treatment. The hens were housed in a convection house for the duration of the study which lasted 52 weeks. Two diets were formulated, one containing 300 g sweet lupins/kg and the other containing sunflower oil cake and fish meal as protein sources. These diets were blended to obtain seven diets with lupin inclusion rates of 0, 50, 100, 150, 200, 250 and 300 g/k.

3. Results indicated that up to 300 g lupins/kg diet can be fed to Hisex laying hens without a significant effect on egg production, egg mass, efficiency of food utilisation, egg shell thickness, Haugh units or yolk colour. Food intake was however, significantly (P<0.05) positively correlated with lupin inclusion rates.     

An improved ferritin assay for canine sera

An improved serum ferritin assay for canine serum has been developed. It uses two monoclonal antibodies in a sandwich arrangement. Serum ferritin can be determined on undiluted canine sera with this assay. The recovery of ferritin added to canine serum ranged from 98 to 106%, the within-assay coefficient of variability was 3.3 to 4.5%, and the assay-to-assay variability was 9.8 to 10.2%. Serum ferritin from 61 apparently healthy dogs had a geometric mean of 252 ng/ml, with a range of 80 ng/ml to 800 ng/ml.     

‘Plantibodies’: a flexible approach to design resistance against pathogens

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.     

Exclusion of infectious diseases from sheep and goat farms

It is important for practitioners and owners to be aware of the risk associated with an open herd. This article has discussed some of the infectious diseases that might be inadvertently introduced. Many others--including most exotic diseases and some that are regulated by governmental agencies, such as tuberculosis and brucellosis--have been omitted. It should be obvious that a simple health certificate is an inadequate guarantee of freedom from infections. Instead, producers should be prepared to invest both time and money in evaluating the health status of the herd of origin. Some of the diseases discussed, such as foot rot, cause tremendous financial losses. Others, such as pinkeye, can be easily "lived with." The point to be made is that producers should consider all these and other diseases and then make informed decisions based on the probable cost of these diseases if they became established in the herd.