Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings

Background  

The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types.     

Shapes of aggregates from tilled layers of some Dutch and Australian soils

Five different methods were used to study the shapes of soil aggregates oriented in their planes of maximum projection. A measure of roundness was found to be relatively insensitive to shape features. The shortest aspect ratio (the ratio of the shortest to the longest diameters through the centroid) was found to be most sensitive of the “single value” shape measures. More comprehensive shape information was given by values of the radius spectra and the curvature spectra. The curvature spectra method seems to be slightly more sensitive than the radius spectra method and can also be applied to reentrant particles.Generally it was found that aggregates are less round with increasing clay content and decreasing organic matter content. There was also a hint that aggregates of the Dutch soils may become less round with increasing age since their reclamation. The Australian soil aggregates were slightly rounder than the Dutch aggregates.     

Multivariate Cluster Analysis Regression Procedures as Tools to Identify Motile Sperm Subpopulations in Rabbit Semen and to Predict Semen Fertility and Litter Size

Computerized motility analysis (CASA) shows that four separate subpopulations of spermatozoa with different motility characteristics co-exist in rabbit ejaculates. There were significant (p < 0.01) differences in the distribution of these subpopulations among separate genetic lines, total sperm abnormalities and the percentage of altered acrosomes. Furthermore, logistic and linear multivariate regressions among several parameters of rabbit semen quality analysis were tested for use as predictive tools for the fertilizing ability of a specific artificial insemination semen sample. Logistic regression analysis rendered two mathematical, significant (p < 0.01) models: one between sperm viability and conception rate and the other between total sperm abnormalities and conception rate. Multiple linear regression analyses also yielded some significant relationships between both fertility (p < 0.001) and litter size (p < 0.05), with respect to some semen characteristics. Our results support the hypothesis that the predictive in vivo fertility use of the standard rabbit semen quality analysis coupled with a CASA determination could be reasonably achieved by applying linear and logistic regression analyses among several parameters of rabbit semen quality analysis.     

Structural stability of two Romanian soils as influenced by management practices

The effects of non-arable vs. arable land use as well as of wheeled traffic, crop rotation, manure applications and inorganic fertiliser application on soil stability parameters were investigated. The stability of a soil's structure was assessed by the dispersibility of clay in water, by measurement of the proportions of water-stable aggregates, and by the tensile strength of dry soil aggregates. An index of soil structural stability is defined and used. In general, there were trends towards increasing soil structural stability with non-arable agriculture, less wheeled traffic, crop rotations rather than monoculture, and fertiliser application.