Molecular cloning and expression of kidney‐type glutaminase from common carp (Cyprinus carpio) and its up‐regulation by glutamine in primary culture enterocyte

Glutaminase (GLS) is the key enzyme of glutamine (Gln) metabolism and utilization. In this study, a cDNA encoding GLS protein was identified from common carp Cyprinus carpio intestine. The open reading frame of GLS cDNA encodes a polypeptide of 595 amino acids, which shows a high similarity with its zebrafish Danio rerio counterpart. Bioinformatic analysis showed the protein belongs to kidney‐type GLS. The putative protein has glutaminase domain and ankyrin repeats domain, which are highly conserved among vertebrate orthologues. Real‐time quantitative PCR analysis revealed that the abundance of GLS mRNA was the highest in the white muscle, followed by the brain, eyeball and pituitary. Glutaminase was ubiquitously expressed in all intestinal segments of common carp. The activity of GLS did not distribute uniformly along the entire length of the intestine. In primary culture enterocyte, and the expression of GLS mRNA is up‐regulated quickly and effectively by Gln.     

青刈黑麦产草量与营养动态分析

青刈黑麦在黄淮海地区具有广阔的发展前景。本文针对棉花冬闲田,对青刈黑麦在不同生育阶段的产草量、营养成分含量、刈割时间及刈割次数进行研究。结果表明,一次利用时,刈割时间不能超过抽穗后期。两次利用时,头茬刈割时间不能超过拔节期,二茬应在抽穗后期刈割。后者草的质量优于前者,但产草量较低,因此在实践中可根据需要决定刈割次数。     

从育人角度谈校园景观设计

校园环境是与学生密切接触的环境,对学生成长起到潜移默化的作用,因此校园环境景观应强调其育人功能。在校园环境景观中纳入以教育为主题的景观,可为学生提供健康的学习环境,培养学生的学习兴趣,扩展学校的实习基地,突出学校的办学特色。以教育为主题的景观有历史人文类、科学知识类、思想道德教育及时事展示类,具有教育的间接性、对外的开放性、受众需求多样性等特点。笔者通过例举实例说明了教育主题景观在校园景观和学校的教育氛围中发挥的重要作用,提出教育主题的校园景观设计需要科学合理地总体规划、细致充分地调查研究、多种教育题材和设计手法,从而营造出可以引导学生学习兴趣、参与性的,与周边建筑协调的,使用功能完善的教育主题景观,以期为今后校园环境中教育主题景观设计提供思路。     

高致病性蓝耳病的检疫检验及无害化处理

2006年夏秋之季至今,我国较大面积发生猪的"高热病",病猪体温很高、呼吸困难、母猪流产、仔猪死亡、传染迅速、病情沉重。给养猪业和肉品安全带来严重危害,使猪只存栏数大幅度减少,猪肉价格明显上涨。农     

百菌清烟剂防治黄瓜霜霉病相关技术试验

研究了保护地条件下百菌清系列烟剂有效成份含量、施药量、施药次数、间隔期4项植保措施与黄瓜霜霉病防治效果的关系。参试4因子中以1次施药量作用最大。最优方案为：百菌清烟剂有效成份含量30％，每次施药量300g／667m2，间隔8d（天），连施4次。     

PEG渗透胁迫下3份大麦材料幼苗叶片抗旱生理特性分析

以甘啤4号、哈瑞特和D- 22大麦为材料,测定不同浓度PEG6000(5％、10％、15％、20％、25％和30％)渗透胁迫处理下大麦幼苗叶片的相对含水量、Pro含量等生理指标,分析PEG渗透胁迫模拟干旱对大麦生理生化特性的影响.结果表明,随着PEG溶液浓度的增加,三个材料的大麦幼苗叶片的相对含水量均下降,而脯氨酸含量...     

柑桔黄龙病菌在贡柑叶片中的分布分析

2017年11月在广东省仁化县4个感染柑桔黄龙病的贡柑园中采叶片样品,进行常规PCR和实时荧光定量PCR检测,从不同病症叶片、典型症状叶片不同部位、初期感病树不同部位(正常和显症)叶片等3个方面分析了贡柑叶片中黄龙病菌"Candidatus Liberibacter asiaticus"(CLas)的分布情况。结果表明,叶片斑驳黄化症和均匀黄化症是与黄龙病最相关的症状,CLas含量很高;缺锌黄化症,具有黄龙病阳性与阴性两类样品;无明显黄化但呈革质化的叶片,CLas含量高。在斑驳黄化与均匀黄化叶片中,CLas的含量从叶尖到叶柄、从叶片到主叶脉呈现递增趋势,即以主叶脉与叶柄的含量更高。初期感病树未显症的部位也会检出黄龙病菌。     

Crop milk protein is synthesised following activation of the IRS1/Akt/TOR signalling pathway in the domestic pigeon (Columba livia)

1. The experiment was conducted to study whether insulin receptor substance 1 (IRS1) / Protein kinase B (Akt)/target of the rapamycin (TOR) signalling pathway activation stimulates crop milk protein synthesis in the domestic pigeon (Columba livia).

2. Crop milk was collected from ten 1-d-old squabs and analysed for nutrient content. During the non-breeding period and the first day of lactation, blood samples were collected from 5 pairs of breeding pigeons and the levels of prolactin and insulin were determined.

3. Crop samples were collected from 5 pairs of breeders at d 14 and 16 of the incubation period and d 1, 3 and 7 of the lactation period. Crop samples were evaluated for changes in crop weight and thickness and changes in the expression patterns of IRS1/Akt/TOR signalling pathway–related proteins.

4. The results demonstrated that prolactin induces a gradual increase in the relative weight and thickness of the crop, with crops reaching a maximum size at the third day of lactation.

5. Pigeon crop milk contains 64.1% crude protein and 29.7% crude fat based on dry weight.

6. Serum prolactin and insulin levels in the lactation period were significantly higher than those in the non-breeding period. Compared with non-breeding pigeons, the expression of the phosphorylated IRS1 phosphorylated Akt, phosphorylated TOR, phosphorylated ribosomal protein S6 kinase, phosphorylated S6, phosphorylated eukaryotic initiation factor 4E binding protein 1 and eukaryotic initiation factor 4E were significantly up-regulated in the crop of pigeons in the lactation period.

7. In conclusion, prolactin might induce changes in crop tissue and form the physiological structure for crop milk synthesis. Furthermore, the synthesis of crop milk protein is regulated by activation of the IRS1/Akt/TOR signalling pathway.

     

推进鄂伦春自治旗农牧业产业化的思考

本文结合鄂伦春自治旗农牧业的物殊现状分析,指出在推进鄂伦春农牧业产业化过程中存在的主要问题,并有针对性地提出相应的对策和建议.