Cytoskeletal changes in <Emphasis Type="Italic">Eimeria bovis</Emphasis>-infected host endothelial cells during first merogony

The first merogony of Eimeria bovis takes place in lymphatic endothelial cells of the ileum, resulting in the formation of macromeronts up to 250 mum. In this study, we investigated the host cell cytoskeleton (actin filaments, microtubules, spectrin, vimentin intermediate filaments) associated with parasitic development in vitro by confocal laser scanning microscopy (CLSM) using primary bovine umbilical vein endothelial cells (BUVEC) and bovine spleen lymphatic endothelial cells (BSLEC) as host cells. No prominent changes in the host cell cytoskeleton were detected 1-3 days after E. bovis sporozoite invasion. With ongoing meront maturation a significant increase in microtubules and actin filaments close to the parasitophorous vacuole (PV) was found. Mature macromeronts within the PV were completely enclosed by these cytoskeletal elements. Our findings suggest, that in order to guarantee the survival of the host cell on the enlargement of macromeronts, E. bovis needs not only to augment but also to rearrange its cytoskeletal system.     

Adapting the CROPGRO model for saline soils: the case for a common bean crop

Water scarcity and severe environmental degradation are causing water managers in the Fergana Valley, Uzbekistan to re-evaluate irrigation water use. Crop models could play an important role in helping farmers decide which systems (crops and irrigation technologies) are feasible. CROPGRO is a physiologically robust agronomic model, although the current version does not consider the effects of soil salinity on crop water use or growth. CROPGRO was modified to include a salinity response function and was tested for gypsiferous soils. A qualitative analysis of the model indicated the model performed as expected under a range of atmospheric, irrigation and crop tolerance scenarios. Model simulations compared very favourably for common bean (Phaseolus vulgaris) to results obtained in the greenhouse for yield and seasonal crop evapotranspiration with values of the Willmott agreement index (i) of 0.98 for both variables evaluated at different levels of salinity and deficit irrigation. Final biomass predictions were less satisfactory, although the modified model performed as well as the original model. The modified model was successfully tested with field data on common bean from an experiment in the Fergana Valley (i of 0.75 for ET and 0.74 for final yield), although the sensitivity of the model to a soil fertility function and relative nodule number made it difficult to assess the model performance.     

The effect of light intensity on growth of juvenile dusky kob Argyrosomus japonicus (Temminck & Schlegel 1843)

South African juvenile dusky kob Argyrosomus japonicus are more abundant in turbid estuaries than in clear marine‐dominated estuaries. Turbidity can reduce light penetration into the water and create an environment different from that experienced by fish under culture conditions in mechanically filtered clear water. In order to optimize rearing conditions of this species, the effects of light intensity (23–315 lx) and feeding method (restricted ration vs. feeding to apparent satiation) on growth and food conversion ratio (FCR) of juvenile A. japonicus were assessed in a 56‐day growth trial. Fish weight increased from 7.2±1.6 to 41.9±10.2 g fish^?1 at a growth rate of 3.25% body weight day^?1. Light intensity did not significantly affect growth or FCR. Feeding method did not significantly affect growth rate, but average FCR was significantly better in treatments fed a ration of 3.6% body weight day^?1 than in treatments fed to apparent satiation. Therefore, a light intensity range of 23–315 lx can be used to culture dusky kob juveniles. The better FCR in fish fed a restricted ration suggests that a ration of 3.6% body mass day^?1 allowed good growth of juvenile dusky kob.     

Pathology of vitamin D deficiency in growing turkeys

Turkey poults were fed a vitamin D-deficient diet and examined for clinical signs and structural changes of bone and parathyroid glands. Vitamin D-deficient poults developed ricketic changes during days 10 to 14. Control poults (deficient diet plus vitamin D) did not develop rickets. In deficient poults, lengths of proliferating-prehypertrophied zones of growth plates increased significantly in the proximal tibiotarsus but were only slightly elongated in the distal tibiotarsus. Unmineralized hypertrophic chondrocyte zones increased in length rapidly in conjunction with a decrease in the length of mineralized hypertrophic degenerative zones; this occurred more rapidly in proximal than in distal tibiotarsus. Other ricketic changes included decreases in bone ash, total femoral bone ash (calcium, phosphorus, magnesium), bone length, and body weight. Plasma alkaline phosphatase was increased, calcium was normal, and phosphorus was normal or elevated. Parathyroids were hyperplastic and had foci of degeneration. Vitamin D3 metabolites 25OHD3, 1,25(OH)2D3, and 24,25(OH)2D3 were rapidly depleted. Increase in bone ash Ca/P ratios in deficient poults suggests that phosphorus may be selectively released from ricketic bone. Low 25OHD3 and 1,25(OH)2D3 of control poults early in the experiment suggests that 1,400 IU of vitamin D3/kg of feed may not be an adequate level of vitamin D3 for growing turkey poults.     

Über Struktur und Funktion des genetischen Materials

Zusammenfassung In dieser zweiten Übersicht sind weitere Literaturergänzungen zum Problem der Struktur und Funktion des genetischen Materials zusammengestellt. Neben gesicherten Fakten wurden auch Hypothesen und unterschiedliche Interpretationen von Untersuchungsergebnissen diskutiert. Die Literatur ist bis November 1964 berücksichtigt.

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Summary In this second review further literature on the problem of structure and function of genetic material are summarized. Beside certain experimental results also hypotheses and different interpretations of results are discussed. The survey of the literature for this review was completed in November 1964.

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am ¶rt;au , , . . 1964 .

     

Über die Proteinbiosynthese und den Genetischen Code

Zusammenfassung In dieser Arbeit sind die Grundlagen der genetischen Information, deren Speicherung und ihre Realisierung in der Proteinbiosynthese zusammenfassend dargestellt. Es wurden die Struktur der Nucleinsäuren, die Replikation der DNS, neuere Ansichten über den Aufbau und die Funktion der Ribosomen sowie die einzelnen Reaktionsschritte bei der Proteinsynthese erläutert. Das letzte Kapitel enthält eine Übersicht von Ergebnissen, die sich mit der Entzifferung des genetischen Code befassen.Trotz der vielen Arbeiten, die in letzter Zeit auf dem Gebiet der Nucleinsäureforschung erschienen sind, bedarf es noch der Sammlung weiteren Tatsachenmaterials, um experimentelle Befunde und bestehende Hypothesen zu einem lückenlosen Bild zu vereinen.

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Summary In this review the basis of genetic information, its accumulation and the realization in protein biosynthesis are summarized. Structure of the nucleic acids, replication of DNA, and recent opinions of the construction and function of ribosomes as well as various steps in protein synthesis are explained. In the last chapter some results on decoding problems are given.In spite of the great number of papers published in the last time further research work will be necessary to synthesize experimental results and existing hypotheses.

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am ¶rt;au , . , , , ¶rt; . , . , , .

     

Feeding 25-hydroxyvitamin D3 to improve beef tenderness

The objective of this trial was to determine if a single oral bolus of 25-hydroxyvitamin D3 (25-OH D3) given at various times before slaughter would enhance the tenderness of beef loin steaks. One hundred eight crossbred steers were allotted to 18 pens so that the mean weight of the cattle in each pen was similar. Treatments (25-OH D3 dose [62.5 or 125 mg]) and time of administration of the single oral bolus (4, 7, 21, or 35 d before slaughter) were assigned randomly to each pen of steers. Serial plasma samples were collected at each bolus administration time for control animals. For steers assigned to a treatment group, a baseline blood sample was collected before bolus administration and at each subsequent administration when other treatment groups received their bolus. Plasma samples were assayed for 25-OH D3 and calcium concentrations. Troponin-T degradation and Warner-Bratzler shear force were measured as indicators of tenderness for loin steaks collected at slaughter and aged for 6 or 14 d postmortem. Muscle samples, collected concurrently, were assayed for 25-OH D3 and calcium concentrations. A single oral bolus of 25-OH D3 was sufficient to increase plasma 25-OH D3 concentrations (P < 0.001) through slaughter, regardless of dose or time of bolus administration. The single oral bolus of 25-OH D3, however, did not increase plasma calcium concentrations (P > 0.05). As a result, neither troponin-T degradation nor Warner-Bratzler shear force was improved (P > 0.05) by treatment. Muscle 25-OH D3 concentrations were increased (P > 0.001) by treatment with 25-OH D3. Although sustained plasma 25-OH D3 concentrations did not increase plasma or muscle calcium at slaughter nor influence tenderness, the use of 25-OH D3 as a nutritional means of improving beef tenderness is in its infancy, and more research to delineate an effective dose and the potential interaction of seasonal exposure to ultraviolet light is warranted.     

Assessing the utility of ALOS PALSAR and SPOT 4 to predict timber volumes in even-aged Eucalyptus plantations located in Zululand,South Africa

In commercial forestry, regular terrestrial enumerations of the growing stock are required for the valuation, sustain-able management and planning of current and future timber supplies. In this study we examined whether the combination of synthetic aperture radar (ALOS PALSAR) and optical satellite (SPOT 4) image data can accurately predict the timber volume of even-aged Eucalyptus plantations located in South Africa. Results from this study show that the combination of ALOS PALSAR and SPOT 4 produces a R ² value of 0.68 for the planted model, whereas the coppiced model produced a R ² value of 0.55. However, by including stand age as an independent variable in the stepwise model, there was a 15% improvement for the planted model, whereas the coppiced model produced a 27% improvement. The final model developed in this study produced a R ² value of 0.83 and a RMSE of 31.71?m³ ha?1 for planted stands, whereas the model for coppiced stands produced a R ² value of 0.82 and a RMSE of 27.70?m³ ha?1. As it is not practical or financially feasible for commercial forestry companies to carry out terrestrial enumerations for all plantations on an annual basis, the model developed in this study presents an alternative and accurate method to calculate timber volume for even-aged Eucalyptus plantations.