Ascorbate levels and the activity of key enzymes in ascorbate biosynthesis and recycling in the leaves of 22 Chinese persimmon cultivars

The objective of this study was to determine diversities of ascorbic acid (AsA) and activities of enzymes involved in AsA metabolism, and to determine their relationships to AsA content in the leaves of Chinese persimmon (Diospyros kaki) cultivars. Results showed that there were huge differences not only in the contents of AsA and glutathione (GSH) but also in the enzyme activities of l-galactono-1,4-lactone dehydrogenase (GLDH, EC 1.3.2.3), dehydroascorbate reductase (DHAR, EC 1.8.5.1), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) in the leaves of 22 Chinese persimmon cultivars. Control of AsA levels mainly depended on the capacity for biosynthesis and GLDH activity played a main role in determining AsA levels in the leaves of persimmon. Both MDHAR and DHAR, MDHAR in particular, also played an important role in maintaining AsA redox state by reducing oxidized AsA back to AsA. But contents of GSH and hydrogen peroide (H₂O₂) and activity of APX were not significantly correlated with AsA content in the leaves of persimmon.     

Protective effect of rAAV2-NTF2 on blood-retinal barrier damage in diabetic rats

AIM: To explore the effect of rAAV2-NTF2 on the blood-retinal barrier damage in early stage diabetic retinopathy. METHODS: Retinal vasculature was observed by Evans blue. NTF2 gene was cloned into adeno-associated virus vector pSNAV. The recombinant pSNAV-NTF2 was transfected into BHK cells. After purification, high-titer rAAV2-NTF2. rAAV2-NTF2 at dose of 4 μL (titer 1.0×1012) were injected into left eyes and rAAV2-EGFP at the same does and titer were injected into the right eyes of 36 rats. After 1 month, diabetes mellitus were induced by STZ. One month after onset of diabetes, EB at a dose of 45 mg/kg was intravenously injected into the rats. Two hours later, both eyes were enucleated immediately after perfusion of 1% PFA-citric acid buffer solution. The retinas were then dissected away and placed in formamide to extract the dye. The concentration of dye was measured by spectrophotometer. RESULTS: Evans blue was contained in normal retinal vessels without leakage, with a very low level of background fluorescence. The vessels staining of diabetic rats’ retina showed increasing fluorescence, indicating the retina-blood barrier damage. Dye concentration, representing the degree of the BRB injury, was higher in retina of 1 month diabetic rats than that in normal rats (4.67 times, P<0.01), indicating retina-blood barrier break-down. Diabetic rats with rAAV2-NTF2 intravitreal injection showed decreased BRB breakdown (P<0.05). CONCLUSION: High expression of NTF-2 decreases the BRB dysfunction in DM rats.     

农业装备物流体系的数学建模

在分析了农业装备物流体系特点的基础上,指出了目前存在的数学模型不能完全体现和描述农业装备物流体系的特点;建立了基于面向环境时延着色Petri网(EOCTPN)的农业装备物流体系数学模型;研究了不同类型农业装备物流体系对库存总成本和长鞭效应的影响程度;解决了目前存在的无法将正向物流和逆向物流有机结合在一起的问题;有效克服了基础Petri网描述此类问题出现的"节点爆炸"现象.在仿真实验的基础上,提出了应如何构建农业装备物流体系的方法.     

关于森林采伐管理改革的思考——以四川省乐山市沐川县为例

结合林木采伐管理情况检查,总结了沐川县森林采伐管理改革的主要做法及取得的成效,并针对存在的问题提出了相应的意见和建议。     

加快推进和完善我国森林生态效益补偿制度的建设

森林资源是陆地生态系统的主体,森林不仅具有重要的经济效益,而且具有重要的环境生态效益。森林资源的建设和保护陷入了缺乏资金长效投入的困境,天保工程等重大工程的实施解决不了长远发展问题,为了保证我国经济发展和环境保护的协调发展,根据我国的现实情况,应制定一部《森林生态效益补偿法》,尽快全国范围内实施森生态效益补偿制度。文章就加快推进和完善我国的森林生态效益补偿制度建设作了初步分析。     

斑茅F1杂交可育亲本选择及其回交后代鉴定和主要经济性状

选用8个Badila(甘蔗热带种)与斑茅属间杂交F1品系与9个甘蔗栽培品种配制了24个回交组合，其中6个F1品系的13个组合杂交可育，共获346株BC1实生苗．经同工酶分析，其中320个株系具有斑茅的特征酶带，为斑茅的真实后代。在杂交可育性和BC1后代主要经济性状方面，斑茅F1品系间和杂交组合问均存在显著差异。斑茅BC1后代的产量性状和糖分仍与甘蔗栽培品种有较大差距，但各性状的最大值已与栽培品种的相当。初步研究结果表明，YC96—40和YC95—41是较好的斑茅F1亲本材料。     

雄性不育及育性恢复基因表达载体的构建

将拟南芥花药特异表达启动子A6与核酸酶基因融合构建不育基因,与核酸酶Bamase特异抑制剂Barstar基因融合构建育性恢复基因,再分别与抗除草剂溴苯睛基因表达盒bxn串联,形成紧密连锁,分别构建植物雄性不育和育性恢复基因表达载体pwP一AN及pwp一AS,以抗澳苯睛基因作为转化和繁殖、制种过程的筛选标记。     

植被盖度对水土保持功效影响的研究综述

就近年来国内有关植被盖度对降雨能量、降雨截留、土壤入渗、初损降雨量、径流量以及泥沙量的影响做了综述;分析了理研究中不足之处,并就今后研究中应注意的问题提出了作者的观点和看法。     

亚精胺处理对大久保桃果实冷敏性的影响

以大久保桃为试材,用不同质量浓度的亚精胺(Spd)浸泡桃果实后进行0～1℃低温贮藏.结果表明,Spd处理一定程度上减轻了冷害的发生;与Spd 100mg/L和Spd 150mg/L相比,Spd 50mg/L对减轻果肉褐变、延缓冷害发生和降低果实冷害程度效果更好.说明适当浓度的亚精胺处理可有效降低大久保桃的冷敏性,延长保鲜期.