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81.
Circulating oxidative stress status in dromedary camels infested with sarcoptic mange 总被引:2,自引:0,他引:2
Oxidative stress is an imbalance between radical-generating and radical-scavenging activity, resulting in oxidation products
and tissue damage. This study was aimed to evaluate the status of oxidative stress indices in blood of camels naturally infested
with S. scabiei. Forty-seven male camels (Camelus dromedaries) were divided according to the extent of the infested area with Sarcoptes scabiei into four groups, mild (MID, n = 12), moderate (MOD, n = 10), severely infested (SEV, n = 10) and healthy control group (n = 15). Blood was used for determination of red cell count (RBC), hemoglobin (Hb), packed cell volume (PCV), serum nitric
oxide (NO•, a free radical), ascorbate and albumin concentrations, and erythrocytic values of malondialdehyde (MDA, a marker of lipid
peroxidation), protein carbonyls (PC, an indicator of protein oxidation), glutathione (GSH) superoxide dismutase (SOD) and
catalase (CAT). Decreased levels (P < 0.05) of RBC, Hb, PCV, albumin and ascorbate were noticed in MOD and SEV compared to controls with the lowest values (P < 0.05) in SEV except for ascorbate, where MOD did not differ from SEV. Compared to controls, NO• gradually increased (P < 0.05) in MID followed by MOD and SEV, whereas MDA and PC were higher (P < 0.05) in MOD and SEV. PC was higher (P < 0.05) in MOD than SEV. In addition, the antioxidants GSH, SOD and CAT were higher (P < 0.05) in MID and lower (P < 0.05) in MOD and SEV compared to controls. GSH was lower (P < 0.05) in SEV compared to MOD. Besides, Hb was negatively correlated with NO• (r = −0.68, P < 0.001), MDA (r = −0.53, P < 0.001) and PC (r = −0.73, P < 0.001). In conclusion, dromedary sarcoptosis is accompanied by a state of oxidative stress process, which increased by
increasing the area of infestation, and may contribute to the pathogenesis of the disease. 相似文献
82.
María Teresa Tejedor-Junco Margarita González-Martín Estefanía Bermeo-Garrido Rebeca Villasana-Loaiza Elena Carretón-Gómez 《Veterinary research communications》2018,42(3):227-232
Doxycycline is an antibiotic that, in addition to the classic antibacterial use, is also prescribed to fight parasitic diseases, like heartworm disease in dogs. Despite the concern that the overuse of this antibiotic may decrease susceptibility of clinically important bacteria, the consequences of the prolonged doxycycline therapy in heartworm-infected dogs have never been studied before. We have analyzed the impact of this therapy on Staphylococcus aureus and Enterococcus antimicrobial resistance. In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates of Staphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal samples (25 before treatment and 23 after treatment). Before treatment, 32% of isolates were resistant at least to one antibiotic while after, this data increase up to 65%. Comparing isolates before and after treatment, a clear increase in resistance to doxycycline (12% against 21.74%) and erythromycin (20% against 39.13%) was observed. Although the present work is a preliminary research, the results encourages the development of further studies to determinate the effect of prolonged doxycycline therapy on antimicrobial resistance. 相似文献
83.
Hosaka Y Kirisawa R Yamamoto E Ueda H Iwai H Takehana K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(10):945-947
Although inflammatory activation of cytokines have been analyzed in various tissues, there have only been a few and as-yet-inconclusive studies on cytokines in equine tendons. In this study, the localizations of 4 cytokines (IL-1alpha, IL-1beta, TNFalpha and IFNgamma) in tendinocytes of the equine superficial digital flexor tendon (SDFT) were analyzed by the use of an immunohistochemical method. In inflamed tendons positive staining for all 4 cytokines antibodies were detected in endotedinieum cells and vascular epithelial cells. In contrast, negative or trace immunoreactions were obtained in many tendinocytes in the normal tendon. The variation in cellular immune responses depending on the kind of cytokine may reflect the physiological/pathological condition of the SDFT. 相似文献
84.
Funaba M Yamate T Hashida Y Maki K Gotoh K Kaneko M Yamamoto H Iriki T Hatano Y Abe M 《American journal of veterinary research》2003,64(8):1059-1064
OBJECTIVE: To evaluate the effects of a high-protein diet versus dietary supplementation with ammonium chloride (NH4Cl) on struvite crystal formation in the urine of clinically normal cats by measuring the urine concentration of hydrochloric acid (HCl)-insoluble sediment, urine pH, struvite activity product (SAP), number of struvite crystals in urine, and urine volume. ANIMALS: 23 healthy adult cats. PROCEDURE: Urine was fractionated by centrifugation with subsequent extraction of the sediment with 1 N HCl (study 1). Diets containing either 29% crude protein or 55% crude protein were fed to cats in a crossover trial of 3 weeks/period (study 2). Diets supplemented with either sodium chloride (NaCl) or NH4Cl were fed, by use of a 3 x 3 Latin-square design with 3 wk/period (study 3). In studies 2 and 3, urine samples were collected for the last 7 days of each period. RESULTS: The HCl-insoluble sediment contained Tamm-Horsfall glycoprotein (THP; study 1). The high-protein diet (study 2) and dietary supplementation with NH4Cl (study 3) resulted in a decrease in urine pH, SAP, and the number of struvite crystals in urine. However, the high-protein diet decreased urine concentrations of HCl-insoluble sediment containing THP (study 2), in contrast to the NH4Cl supplementation that increased urine volume without a significant effect on the urine concentration of the HCl-insoluble sediment (study 3). CONCLUSIONS AND CLINICAL RELEVANCE: Our results indicate that compared with dietary supplementation with NH4Cl, the high-protein diet is preferable as a urine acidifier for the prevention of struvite crystal formation in clinically normal cats. 相似文献
85.
Tatsuya Nakayama Michio Jinnai Ryuji Kawahara Khong Thi Diep Nguyen Nam Thang Tran Thi Hoa Le Kieu Hanh Pham Ngoc Khai Yoshinori Sumimura Yoshimasa Yamamoto 《Tropical animal health and production》2017,49(1):31-37
Reports of livestock infections with extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E) are increasing. Based on interviews conducted over a 6-month period, we found that veterinarians in the Vietnamese province of Thai Binh prefer to prescribe colistin-based drugs (CBD) in chicken farms. We aimed to clarify whether CBD use selects for strains of colistin-resistant ESBL-E. With the cooperation of seven local households, we detected ESBL-E in chickens’ feces after treating chickens with CBD. Phylogenetic groupings and the presence of CTX-M/AmpC genes were determined, and the multi-antibiotic susceptibility of isolates was analyzed. Our results showed that ESBL-E presented in seven chickens’ feces from two households. Seventy-two percent of ESBL-E isolates harbored CTX-M9 and the phylogenetic group A; the colistin minimum inhibitory concentration (MIC) of all isolated ESBL-E ranged from 0.064 to 1 μg mL?1. Moreover, ESBL-E isolates were used to experimentally select for colistin resistance, and the effect of commercial CBD on ESBL-E was investigated. The results showed that an ESBL-E strain with a colistin MIC of 4 μg mL?1 was able to grow in media with CBD. Although CBD treatment was effective, in vitro experiments demonstrated that ESBL-E can easily acquire colistin resistance. Therefore, restrictions on colistin use are necessary to prevent the emergence of colistin-resistant bacteria. 相似文献
86.
Ouédraogo-Koné S Kaboré-Zoungrana CY Ledin I 《Tropical animal health and production》2008,40(2):155-164
Foliage of Afzelia africana, Pterocarpus erinaceus and Khaya senegalensis, from 10 trees per species, was collected every two weeks during the late dry, rainy and cool season to determine the seasonal
effects on chemical composition. Fifteen rams of the Djallonké breed, weighing on average 20.0 kg, were used to evaluate the
voluntary intake and digestibility of hay of A. gayanus, foliage of A. africana (as a sole feed), and A. africana, P. erinaceus and K. senegalensis offered with 30% of the diet as A. gayanus hay. The crude protein (CP) content of A. africana, and P. erinaceus decreased significantly from the late dry season to the cool season when that of K. senegalensis tended to increase. The mean CP of A. africana, P. erinaceus and K. senegalensis differed significantly (173 g, 139 g and 114 g/kg DM, respectively). The DM intake of A. africana offered with hay (571 g/d) or as a sole feed (598 g/d) were not significantly different, but was higher than that of P. erinaceus (428 g/d) and K. senegalensis (298 g/d). The digestibility calculated by difference of DM and CP of A. africana (582 g/kg DM and 795 g/kg CP, respectively) did not differ significantly from A. africana as a sole feed, but were higher than for the other species. The nutritive value of A. africana seems to justify the high preference of herders for this species. 相似文献
87.
S. Kennou Sebei R. Bergaoui M. Ben Hamouda R. G. Cooper 《Tropical animal health and production》2009,41(7):1427-1438
The aim of the current investigation was to determine wild ostrich reproductive behaviour in Orbata Nature Reserve by observing
16 hens and 28 cocks over a seven-year period. Intense laying commenced in January, one month after the cessation of the rainy
season, and 92% of the eggs were produced during the dry season (January to May, peaking in March). Over the seven years,
1,322 eggs were laid in 69 nests, which corresponded to an annual average production of 19.2 ± 9.1 eggs/nest and 11.8 eggs/hen.
24 nests (34.78%) were non-brooded, 17 nests (24.64%) were deserted in the course of incubation, and 28 nests (40.58%) possessed
hatched eggs. All the non-incubated nests had egg losses equivalent to 46.6 ± 12.6%. Hatchability success of incubated eggs
was 41.9 ± 12.0%. Ostriches tended to dig their nests adjacent to the reserve enclosure which had direct access by road and
track, the latter subjecting them to human disturbance and predation. The systematic obstruction of these nests stimulated
ostriches to build additional nests within the reserve perimeter. The authors discussed the results recorded in an ostrich
flock in relation to the environmental factors (climatic factors, food disponibility and predation) and suggested possibilities
for improved wildlife management. 相似文献
88.
Hiromi KEINO Atsushi FUNAHASHI Hiroomi KEINO Chihiro MIWA Masanori HOSOKAWA Yoshihiro HAYASHI Kenji KAWAKITA 《Journal of Equine Science》2009,20(4):79-88
In this study, we applied a novel psycho-educational horseback riding (PEHR) program to
the treatment of four Japanese children with pervasive developmental disorders (PDD) in
order to facilitate the acquisition of verbal and nonverbal communication skills. The
behavioral changes in each child were evaluated using a psychological and behavioral
scale. The scale for evaluating the effect of Human-Equips-Interaction on Mental activity
(HEIM scale) was designed to assess the behavioral improvement of children based on the
following 10 items: Human relationships, Imitation, Emotional expression, Sudden physical
movement, Fixative behavior, Adaptation to change, Visual response, Fear or nervousness,
and Verbal and nonverbal communication. After taking part in the PEHR program for several
months, all subjects showed remarkably improved HEIM scores and marked improvements were
observed in eye contact with others (instructors, side walkers, and leaders) in the riding
area. A statistical difference was found in items 1, 2, 3, 6, 7, 8, and 9. However, no
statistical difference was found in items 4, 5, and 10. As the program progressed, the
children showed enhanced verbal and nonverbal communication skills, and became more
expressive in their emotional and empathetic interaction with their parents. These
observations suggest that the normal functioning of pleasurable emotions and empathy may
facilitate further improvements in joint attention, imitation and empathy, and may result
in successful verbal expression by PDD children. Therefore, horseback riding can play a
very important role in the psycho-educational support required for the communication
ability of PDD children. 相似文献
89.
Effects of tea saponins on rumen microbiota,rumen fermentation,methane production and growth performance—a review 总被引:1,自引:0,他引:1
Reducing methane emission from ruminant animals has implications not only for global environmental protection but also for
efficient animal production. Tea saponins (TS) extracted from seeds, leaves or roots of tea plant are pentacyclic triterpenes.
They have a lasting antiprotozoal effect, but little effect on the methanogen population in sheep. There was no significant
correlation between the protozoa counts and methanogens. The TS decreased methanogen activity. It seems that TS influenced
the activity of the methanogens indirectly via the depressed ciliate protozoal population. The TS addition decreased fungal
population in the medium containing rumen liquor in in vitro fermentation, but no such effect was observed in the rumen liquor
of sheep fed TS. Tea saponins had a minor effect on the pattern of rumen fermentation and hence on nutrient digestion. When
added at 3 g/day in diets, TS could improve daily weight gain and feed efficiency in goats. No positive associative effect
existed between TS and disodium fumarate or soybean oil on methane suppression. Inclusion of TS in diets may be an effective
way for improving feed efficiency in ruminants. 相似文献
90.
Millogo V Norell L Ouédraogo GA Svennersten-Sjaunja K Agenäs S 《Tropical animal health and production》2012,44(5):1017-1025
The aim of the study was to test three different hand-milking techniques (“pull down”, “thumb in”, and “full hand grip”) and
their effects on milk production and teat treatment. This is important since milk production in many tropical areas still
rely on hand-milking. The study was carried out at a peri-urban farm in the Bobo-Dioulasso area in Burkina Faso. Twelve indigenous
Zebu cows in early lactation were used in the study. The sequences of the treatments (hand-milking techniques) and the milkers
were balanced for carry-over effects between successive periods and days, respectively. The sequences were constructed by
using special Latin squares. Yield and composition of saleable milk was not affected by milking technique but there were differences
between the milkers in milk yield. There was also a significant interaction for saleable milk yield between milker and milking
technique. Hemoglobin in milk was measured as an indicator of teat damage. The hemoglobin content was numerically higher in
post-milking strip milk samples than in saleable milk. It was concluded that the amount of milk removed depends mainly on
the milker and how well the milking technique works for the individual milker. No effect of milking technique was observed
on teat treatment. 相似文献