全文获取类型
收费全文 | 212篇 |
免费 | 1篇 |
国内免费 | 1篇 |
专业分类
林业 | 3篇 |
农学 | 11篇 |
46篇 | |
综合类 | 11篇 |
农作物 | 6篇 |
水产渔业 | 11篇 |
畜牧兽医 | 121篇 |
园艺 | 2篇 |
植物保护 | 3篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 8篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 22篇 |
2012年 | 12篇 |
2011年 | 16篇 |
2010年 | 8篇 |
2009年 | 15篇 |
2008年 | 15篇 |
2007年 | 17篇 |
2006年 | 19篇 |
2005年 | 14篇 |
2004年 | 10篇 |
2003年 | 6篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1989年 | 2篇 |
1982年 | 1篇 |
排序方式: 共有214条查询结果,搜索用时 0 毫秒
91.
Arisa SUGIMOTO Hitomi TSUCHIDA Mayuko NAGAE Naoko INOUE Yoshihisa UENOYAMA Hiroko TSUKAMURA 《The Journal of reproduction and development》2022,68(3):190
Reproductive function is suppressed during lactation owing to the suckling-induced suppression of the kisspeptin gene (Kiss1) expression in the arcuate nucleus (ARC) and subsequent suppression of luteinizing hormone (LH) release. Our previous study revealed that somatostatin (SST) neurons mediate suckling-induced suppression of LH release via SST receptor 2 (SSTR2) in ovariectomized lactating rats during early lactation. This study examined whether central SST-SSTR2 signaling mediates the inhibition of ARC Kiss1 expression and LH release in lactating rats during late lactation and whether the inhibition of glutamatergic neurons, stimulators of LH release, is involved in the suppression of LH release mediated by central SST-SSTR2 signaling in lactating rats. A central injection of the SSTR2 antagonist CYN154806 (CYN) significantly increased ARC Kiss1 expression in lactating rats on day 16 of lactation. Dual in situ hybridization revealed that few ARC Kiss1-positive cells co-expressed Sstr2, and some of the ARC Slc17a6 (a glutamatergic neuronal marker)-positive cells co-expressed Sstr2. Furthermore, almost all ARC Kiss1-positive cells co-expressed Grin1, a subunit of N-methyl-D-aspartate (NMDA) receptors. The numbers of Slc17a6/Sstr2 double-labeled and Slc17a6 single-labeled cells were significantly lower in lactating dams than in non-lactating rats whose pups had been removed after parturition. A central injection of an NMDA antagonist reversed the CYN-induced increase in LH release in lactating rats. Overall, these results suggest that central SST-SSTR2 signaling, at least partly, mediates the suppression of ARC Kiss1 expression and LH release by inhibiting ARC glutamatergic interneurons in lactating rats. 相似文献
92.
Combined treatment of aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) reduces melting-flesh peach fruit softening 总被引:1,自引:0,他引:1
The effects of postharvest application of aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) on ethylene production and fruit quality, and thus on transportation and shelf-life, were evaluated in melting-flesh peaches. AVG (150 mg L−1) significantly reduced ethylene production, and the effect was enhanced in combination with 1-MCP (1 μL L−1). However, fruit treated with AVG alone softened to untreated control levels 2 d after harvest (DAH). Treatment with 1-MCP significantly reduced the rate of softening until 2 DAH, but the fruit rapidly softened thereafter, and reached untreated control levels by 4 DAH. A combination of AVG and 1-MCP significantly reduced fruit tissue softening throughout ripening. The effect of each chemical on flesh firmness indicated that 1-MCP affected fruit response in the early stages of ripening up to 4 DAH, and AVG significantly reduced softening in the latter stages from 4 to 9 DAH. Peaches treated with AVG and 1-MCP retained their ground color during ripening, but the effect of each chemical on color is unclear. The present study indicates that combined treatment with AVG and 1-MCP significantly delays the ripening of melting-flesh peaches. 相似文献
93.
94.
Noritake K Matsuoka T Ohsawa T Shimomura K Sanbuissho A Uenoyama Y Maeda K Tsukamura H 《The Journal of reproduction and development》2011,57(3):409-415
It has recently been shown that neurokinin B, a tachykinin, is associated with GnRH pulse generation in sheep and goats. The aim of the present study was to clarify the role of tachykinin receptors in the control of LH secretion in rats. To this end, we evaluated the effect of CS-003, an antagonist for all three neurokinin receptors (NK1, NK2 and NK3 receptors), on pulsatile LH secretion in both sexes of rats with different routes of administration. Both oral and third ventricular administration of CS-003 suppressed LH secretion in both sexes of gonadectomized animals. Furthermore, intact male rats with oral administration of CS-003 showed decreased serum testosterone levels, which might be due to suppressed LH secretion. None of the three subtype-specific neurokinin receptor antagonists showed a significant effect on LH secretion in ovariectomized rats when each antagonist was singly administered. The present results suggest that neurokinins play a role in the control of pulsatile GnRH/LH secretion via multiple neurokinin receptors in both male and female rats. 相似文献
95.
Reconstruction of stratum corneum in organotypically cultured canine keratinocyte-derived CPEK cells
Yagihara H Okumura T Shiomi E Shinozaki N Kuroki S Sasaki Y Ito K Ono K Washizu T Bonkobara M 《Veterinary research communications》2011,35(7):433-437
The stratum corneum of epidermis is an essential barrier against the external environment and water loss. This study aimed
to develop an organotypic culture model that targets the reconstruction of the stratum corneum using canine keratinocyte-derived
CPEK cells. The CPEK cells cultured at the air-liquid interface became stratified and formed a stratum corneum-like layer
on stratum spinosum- and stratum granulosum-like layers. The CPEK cells in the stratum granulosum-like layer expressed the
cornified cell envelope (CCE)-related proteins loricrin and keratinocyte differentiation-associated protein. Organotypically
cultured CPEK cells were considered to form a CCE at the stratum granulosum-like layer, allowing the formation of a stratum
corneum-like layer. The organotypic culture of CPEK cells could be useful for studying the barrier function of canine stratum
corneum. 相似文献
96.
Dehal P Satou Y Campbell RK Chapman J Degnan B De Tomaso A Davidson B Di Gregorio A Gelpke M Goodstein DM Harafuji N Hastings KE Ho I Hotta K Huang W Kawashima T Lemaire P Martinez D Meinertzhagen IA Necula S Nonaka M Putnam N Rash S Saiga H Satake M Terry A Yamada L Wang HG Awazu S Azumi K Boore J Branno M Chin-Bow S DeSantis R Doyle S Francino P Keys DN Haga S Hayashi H Hino K Imai KS Inaba K Kano S Kobayashi K Kobayashi M Lee BI Makabe KW Manohar C Matassi G Medina M Mochizuki Y Mount S 《Science (New York, N.Y.)》2002,298(5601):2157-2167
The first chordates appear in the fossil record at the time of the Cambrian explosion, nearly 550 million years ago. The modern ascidian tadpole represents a plausible approximation to these ancestral chordates. To illuminate the origins of chordate and vertebrates, we generated a draft of the protein-coding portion of the genome of the most studied ascidian, Ciona intestinalis. The Ciona genome contains approximately 16,000 protein-coding genes, similar to the number in other invertebrates, but only half that found in vertebrates. Vertebrate gene families are typically found in simplified form in Ciona, suggesting that ascidians contain the basic ancestral complement of genes involved in cell signaling and development. The ascidian genome has also acquired a number of lineage-specific innovations, including a group of genes engaged in cellulose metabolism that are related to those in bacteria and fungi. 相似文献
97.
Chen T Hiroko T Chaudhuri A Inose F Lord M Tanaka S Chant J Fujita A 《Science (New York, N.Y.)》2000,290(5498):1975-1978
Diploid yeast cells repeatedly polarize and bud from their poles, probably because of highly stable marks of unknown composition. Here, Rax2, a membrane protein, was shown to behave as such a mark. The Rax2 protein itself was inherited immutably at the cell cortex for multiple generations, and Rax2 was shown to have a half-life exceeding several generations. The persistent inheritance of cortical protein markers would provide a means to couple a cell's history to the future development of a precise morphogenetic form. 相似文献
98.
99.
The toadfish,Opsanus tau, lacks renal glomeruli and macula densa, but has high renal renin activity and abundant granulated cells in renal arteries
and arterioles. Reduction of blood pressure (BP) or blood volume by hemorrhage or vasodilatory drugs causes renin release,
indicating that an intrarenal or extrarenal pressure- or volume-sensitive mechanism exists for controlling renin release in
the toadfish. Thus, we examined whether 1) β-adrenergic receptor-mediated activation of renin release, and 2) calcium influx
which may underlie the baroreceptor mechanism are involved in the cellular control of renin release. Acute injection of isoproterenol
(1 μg/kg, n = 6) decreased BP and increased plasma renin activity (PRA) 4–5 fold in unanesthetized toadfish. Propranolol abolished
both effects, but did not decrease basal PRA levels.In vitro superfusion of renal slices with bicarbonate Ringer's solution showed a steady secretion of renin, and addition of 50 mM
K+ (K+ methylsulfate replacing NaCl, n = 10) to the superfusate markedly suppressed renin secretion. Nifedipine (10−5 M, n = 8) completely restored the high K+-induced inhibition of renin secretion from renal slices, whereas isoproterenol (10−4 M, n = 6) neither increased basal renin secretion nor restored K+-induced renin suppression. These results suggest that calcium influx may mediate inhibitory messages for renin secretion,
while the β-adrenoceptor-mediated activation of granulated cells appears absent in toadfish. 相似文献
100.
Yousuke NANIWA Keisuke NAKATSUKASA Shohei SETSUDA Shinya OISHI Nobutaka FUJII Fuko MATSUDA Yoshihisa UENOYAMA Hiroko TSUKAMURA Kei-ichiro MAEDA Satoshi OHKURA 《The Journal of reproduction and development》2013,59(6):588-594
Kisspeptin is a key molecule that stimulates gonadotropin secretion via release of
gonadotropin-releasing hormone (GnRH). In the present study, our aim was to
investigate whether kisspeptin has stimulatory effects on follicular development via
GnRH/gonadotropin secretion in cows. Japanese Black beef cows were intravenously
injected with full-length bovine kisspeptin [Kp-53 (0.2 or 2 nmol/kg)] or vehicle 5
days after they exhibited standing estrus (Day 0). In cows injected with Kp-53 at 2
nmol/kg, the follicular sizes of the first dominant follicles increased on Day 6 and
thereafter. Ovulation of the first dominant follicle occurred in 1 out of 4 cows
treated with Kp-53 at 2 nmol/kg. Injection of Kp-53 at 2 nmol/kg increased the
concentration of plasma luteinizing hormone (LH) but not follicle-stimulating
hormone, over a 4-h period following injection in all cows. The present study
suggests that administration of full-length kisspeptin causes LH secretion, which is
sustained for a few hours, and it is capable of stimulating follicular development
and/or ovulation. 相似文献