Upstream migration mechanisms of juvenile temperate sea bass <Emphasis Type="Italic">Lateolabrax japonicus</Emphasis> in the stratified Yura River estuary

Hydrographic conditions and distributions of juvenile temperate sea bass Lateolabrax japonicus were observed in early spring from 2009 to 2012 in the Yura River estuary, which is highly stratified due to its small tides and consequent seawater intrusion into the bottom layer of the river as a salt wedge. In all four studied years, the upstream expansion of their distribution coincided with the timing of the salt wedge intrusion from the lower to upper estuary, indicating that juvenile fish used salt wedge intrusions to ascend the estuary in early spring. However, juveniles sometimes remained in the nearshore area even when the salt wedge intrusion had already occurred, indicating that other triggers are also likely to be necessary. We therefore evaluated the effects of temperature on upstream migration behaviors. The relationship between the mean temperature they experienced from hatch until starting the ascent and mean age of each cohort at the upstream migration fitted with the law of effective cumulative temperature. Most cohorts ascended the river at an effective cumulative temperature of approximately 500 °C–days. This suggests that higher temperatures would lead to a shorter period prior to the upstream migration.     

Amberjack <Emphasis Type="Italic">Seriola dumerili</Emphasis> interleukin-10 negatively suppresses host cell-mediated immunity

Interleukin-10 (IL-10) is an anti-inflammatory cytokine and negatively regulates cell-mediated immunity (CMI) induction by inhibiting cytokine production in type 1 T helper cells. IL-10 genes have been isolated from several fish, and inflammatory cytokine inhibition by IL-10 has been well examined. However, a CMI regulator of IL-10 in fish has not yet been identified. In this study, we cloned the IL-10 gene in amberjack Seriola dumerili and analyzed its function using its recombinant protein (rIL-10). In an in vitro culture experiment, gene expression of inflammatory cytokines was suppressed in leukocytes incubated with rIL-10 compared with cells that only received Nocardia seriolae stimulation. This result suggests amberjack IL-10 has conserved function as an inflammatory cytokine inhibitor. Bactericidal activity of amberjack cells against intracellular pathogen stimulation was decreased in a rIL-10 dose-dependent manner. Furthermore, a significant reduction in the T-bet/GATA-3 ratio was observed in N. seriolae living cell (LC)?+?rIL-10-injected fish. Taken together, these results suggest amberjack rIL-10 suppresses CMI induction both in vitro and in vivo. In addition, the number of IgM⁺ cells among spleen leukocytes in N. seriolae?+?rIL-10-injected fish was higher than in only N. seriolae LC, suggesting that Th2-dominant immunity was induced by adding rIL-10.     

Effect of body weight on the chemical composition and collagen content of snakehead fish <Emphasis Type="Italic">Channa striata</Emphasis> skin

Snakehead fish (Channa striata) skin is a fishery by-product that has the potential for further processing because it contains a high amount of organic matter. This study investigates the effect of body weight on the chemical composition and collagen content of snakehead fish skin. This study used fresh snakehead fish of either gender. Their body weights were divided into three groups: small, medium, and large size. The characteristics of snakehead fish skin included proximate composition, amino acid content, collagen content, microstructure, and minerals. Snakehead fish skin from fish of different body weights indicated that the moisture content and ash tended to decrease, the protein content was relatively stable, and the lipid content tended to increase with increasing body weight. Glycine and proline comprised the highest percentages of amino acids at all levels of body weight, and the presence of hydroxyproline showed that snakehead fish skin was the source of collagen. There was no significant difference in body weight observed on collagen protein content of the skin. This study on the microstructure and mineral content of snakehead fish skin can be used as supporting information to promote the potential utility and economic value of the skin.     

Genetic identification of native populations of fluvial white-spotted charr <Emphasis Type="Italic">Salvelinus leucomaenis</Emphasis> in the upper Tone River drainage

ABSTRACT:   Stocking of exogenous, hatchery-reared white-spotted charr Salvelinus leucomaenis has been conducted throughout much of their range in Honshu Island, Japan, to increase angling opportunities. Although the native charr populations are thought to have declined because of hybridization with introduced fish, their distribution and genetic status have been uncertain. Fine population structures of charr in the upper Tone River drainage were examined using mitochondrial DNA and microsatellite analyses so as to clarify the presence of native populations. One common mtDNA haplotype was detected in all populations in the Ohashi River and Watarase River, and four and one tributary populations were monomorphic for such haplotypes, respectively. However, several haplotypes, considered to have originated from stocked hatchery fish, were observed in the stocked and the remaining populations. Judging from the genetic integrity over a fine geographic scale, the former were considered as indicative of native populations and the latter as admixtures with hatchery fish. Comparisons of genetic diversity, deviations from the Hardy–Weinberg equilibrium, principal component analysis, and relatedness estimations based on microsatellite DNA can also provide evidence for distinguishing native populations from those influenced by hatchery fish.     

Avian predation on wild and cultured sea urchin <Emphasis Type="Italic">Strongylocentrotus intermedius</Emphasis> in a rocky shore habitat

ABSTRACT:   The present study reports the annual variation in consumption of the sea urchin Strongylocentrotus intermedius by avian predators on a rocky shore where the culture of sea urchins has been conducted. Carrion crow and a few gull species were the most abundant avian predators and consumed a large number of sea urchins. Crows consumed mostly natural sea urchins, approximately 36 kg ww/ha per year on the intertidal rocky bench, but the gull species consumed mostly cultured sea urchins, approximately 100 kg ww/ha per year in the culture area. The seasonal variation in the amount of sea urchins consumed by crows was higher than that by the gull species, presumably because of the difference in foraging behavior in association with the seasonal tidal cycle. The natural sea urchins consumed are an allochthonous input from the subtidal to the intertidal habitat, and thus, crow predation may not affect the natural and the cultured populations of the sea urchin. The gull species consumed much of the cultured sea urchin, and thus, may be regarded as an effective predator causing damage to sea urchin culture. The results suggest that further studies are needed to determine why the gull species selectively feed on cultured sea urchins.     

Recording cerebellar neuron activities in swimming goldfish

ABSTRACT:   Neuronal activities were investigated in the cerebellum of immobilized and swimming goldfish Carassius auratus . Extracellularly recorded neural activities of the cerebellum in immobilized goldfish were characterized and classified into five types. Based on the waveforms and recording depths, these five neural activity types were estimated to originate from three identified classes of cerebellar neurons: Purkinje cells, eurydendroid cells, and granule cells. Chronic recording of cerebellar neuron activities in unrestrained goldfish was performed for more than 100 h. During the chronic recordings, a submersible amplifier attached to the goldfish head, and a multielectrode array developed for the present study were used. Neuronal activities in the cerebellum of free-swimming fish could also be classified into five types as in the immobilized condition. Firing patterns of two neurons identified as Purkinje cells and eurydendroid cells were analyzed during turning movements of the goldfish. The firing patterns of these neurons changed in relation to turning movements. Although some improvements are required, the chronic recording method developed in the present study can be applied to further investigations concerning the direct relationship between brain neural activities and certain behavior.     

Selectivity of flexible size-sorting grid in Mediterranean multispecies trawl fishery

ABSTRACT:   The demersal multispecies trawl fishery in the western Mediterranean Sea has a poor selectivity; large numbers of juvenile fish are caught when using the legislated 40-mm diamond mesh cod end. The selectivity of a flexible sorting grid with 20-mm bar spacing (BS20) installed in front the conventional trawl cod end was investigated. The standard covered cod end method was used. Data was adequate for analyzing the selectivity of European hake Merluccius merluccius , poor cod Trisopterus minutus , greater forkbeard Phycis blennoides and Norway lobster Nephrops norvegicus . The selectivity of the 20-mm sorting grid was compared with the performance of 40-mm diamond (DM40) and square mesh (SM40) cod ends. The effect of a guiding funnel on the performance of the grid (BS20-f) was also explored. Finally, the potential changes in yield per recruit (Y/R) and biomass per recruit (B/R) after implementing a sorting grid were explored. An overall improvement in the 50% selection length ( L ₅₀ ) with all four species was substantial when comparing the BS20 to the DM40. Higher improvement in L ₅₀ s was achieved when the grid was equipped with the guiding funnel. When comparing the performance of the BS20-f to the SM40, there was no marked difference in L ₅₀ s. The Y/R and B/R, however, are substantially higher with BS20-f for poor cod and about the same for European hake. BS20 compared to SM40 achieved lower Y/R and B/R for all four species; the smallest difference was observed for Norway lobster. Further development is required if the sorting grid is to be introduced into commercial fisheries.