家蚕有滞育多化性品种滞育卵复式冷藏继代法的研究

利用上年中秋制种的滞育卵经复式冷藏处理,到当年夏蚕期饲养,夏制种再经中秋蚕期饲养,进行了有滞育多化性品种的省力保种试验.夏蚕期其生命力较对照组低1.2%~1.9%,生种率上升16.0%,差异达显著程度.中秋蚕期生命力高1.0%,生种率低0.9%,完全恢复正常.重复试验,结果一致.有滞育多化性品种用滞育卵复式冷藏处理一年二次省力保种是可行的.     

3个家蚕品种部分产茧量及体质量性状指标的协方差分析

养蚕生产中家蚕的桑叶食下量难以人为控制在同一水平,通常认为食下量会对蚕体生长发育及全茧量等产茧量指标产生显著甚至极显著影响。以山东省现行的3个春用蚕品种为材料,将食下量作为协变量进行协方差分析的结果表明,3个家蚕品种在3-5龄的蚕体质量增量、全茧量、茧层量性状指标上无统计学意义(P>0.125 2),仅个别指标均值间的最大差异在0.1水平下显著(P全茧量=0.066 9,P茧层量=0.056 1),因此该方法校正了用常规方差分析得出的上述性状指标在品种间存在极显著差异(P<0.000 1)的有偏结论,还明确了消化吸收量对上述性状指标影响不显著,可不作为协变量考虑。     

民族医药治疗慢性乙肝的研究进展

乙型肝炎病毒(HBV)是感染人类最常见的病毒性疾病之一,在慢性患者中每年发生肝硬化和肝细胞癌率分别占2%和1%.因此,乙型肝炎病毒严重威胁着人类的生命健康.我国各个民族对乙肝的治疗均有独特的方法,且效果明显,具有很大的开发潜力.笔者就近年来有关乙肝治疗的民族医药的研究及其应用情况作一概述.     

关于强化我国动物防疫体系的几点思考与建议

建立健全动物防疫体系既是有效防控非洲猪瘟等重大动物疫病的迫切需要,也是促进畜牧业持续健康发展、维护人民群众身体健康和动物产品质量安全的必然要求。但目前,我国动物防疫体系面临工作力量不足、基层队伍不稳、运行机制不畅、保障水平不够等问题,难以适应控制和消灭重大动物疫病、保障生猪等重要畜禽产品稳产保供的新形势新要求。建议从突出强化基层动物防疫体系、建立健全兽医卫生社会化服务体系、加快动物防疫智能化转型等三个方面发力,积极构建适合中国国情的新型动物防疫体系,切实维护畜牧业生产安全和公共卫生安全。     

饲养方式对蛋鸡产蛋前期生产性能的影响

试验通过对比舍外散放蛋鸡与舍内笼养蛋鸡在体重、胫长和胫围等方面的差异来研究饲养方式对鸡产蛋前期生产性能的影响.结果表明:散放鸡体重在8、10、12周龄显著低于同周龄笼养鸡;散放鸡胫长在10、12周龄显著低于同周龄笼养鸡;散放鸡胫围在10周龄显著低于同周龄笼养鸡.笼养鸡和散放鸡各周龄的胫长和胫围变异系数均小于10%,均匀...     

硫酸新霉素可溶性粉在靶动物猪的安全性研究

为了客观评价硫酸新霉素可溶性粉对靶动物猪临床应用的安全性,将32头仔猪随机分为4组,每组8头,分别设1倍、3倍、5倍临床推荐剂量组和空白对照组,连续混饮硫酸新霉素可溶性粉15 d,通过检测各组受试猪的临床表现、血液常规指标、血清生化指标、尿液常规指标及病理学变化等评价其临床用药的安全性。结果:硫酸新霉素可溶性粉以5倍临床推荐剂量内应用于靶动物猪后,对其临床症状、体重、血常规、血生化及尿常规等指标没有显著影响,解剖学及病理组织学检查也未见任何病理变化,说明硫酸新霉素可溶性粉以1倍、3倍、5倍临床推荐剂量应用于靶动物猪是安全的。     

玫瑰花再制奶酪的研制

本研究充分利用云南省丰富的玫瑰资源,在再制奶酪基础上,添加含有功能活性物质的玫瑰花发酵液与玫瑰花瓣,研制出方便携带且适合国人口味的压片式再制甜奶酪。通过测定发酵玫瑰花中的活性物质,确定玫瑰花的适宜发酵时间;以感官评分为指标,筛选添加剂的合适添加量;在单因素的基础上应用响应面优化发酵工艺;通过测定产品的理化、卫生指标,评估产品质量。结果表明:当玫瑰花发酵至第6天活性物质含量较高;添加12%脱脂奶、3.5%黄油、12%白糖、2%复合磷酸盐、0.5%黄原胶时,产品的感官评分较高;经优化得到当搅拌温度为87℃、玫瑰花发酵液添加量为20%、发酵玫瑰花添加量为6.5%时,产品感官评分较高(80.84分),与预测值接近;理化和微生物指标检测结果显示,本产品的脂肪、水分含量及酵母菌和霉菌数、菌落总数、大肠菌群数均符合标准。     

Cardiomyopathy in Dystrophin-Deficient Hypertrophic Feline Muscular Dystrophy

A colony of cats affected with hypertrophic feline muscular dystrophy was used to study the occurrence of cardiomyopathy associated with dystrophin deficiency. Affected male and female cats, obligate carrier females, and unaffected healthy littermates were followed from 12 weeks of age into adulthood. Thoracic radiography, 2-D echocardiography, and 2-D-derived M-mode echocardiography were performed at 3-month intervals until 12 months of age and regularly thereafter. From 9 months of age, all affected cats had larger hearts than normal and carrier animals. Left ventricular wall thickness in systole and in diastole and interventricular septal thickness in systole were greater in affected cats 12 months and older when compared with normal or heterozygous animals (P < .05). The myocardium of affected cats was diffusely hypoechoic and thickened. Multiple hyperechoic foci were in the myocardium and papillary musculature. Shortening fraction was normal in all cats. Changes seen in carrier females included enlargement and hyperechogenicity of the papillary musculature after the age of 2 years. Gross and light microscopic examination revealed left ventricular wall thickening with multiple foci of mineralization in 2 of 5 hearts from dystrophin-deficient cats. Although approximately 10% of the normal dystrophin amount was present in the skeletal muscle, dystrophin could not be detected in the myocardium. Early onset concentric myocardial hypertrophy was present in all adult cats. Lesions were mainly localized in the myocardium of the left ventricular free wall and interventricular septum, papillary musculature, and the endocardium. Clinical signs of heart failure developed only infrequently in cats with hypertrophic feline muscular dystrophy.     

猪外周血单个核细胞增殖反应影响条件的探讨

应用 Ｌ１６ （４５） 正交试验, 对影响猪 Ｐ Ｂ Ｍ Ｃ 增殖反应的几个因素, 包括培养时间、 Ｐ Ｈ Ａ 浓度、细胞浓度等三个因素四个水平进行了比较和探索。试验表明几个因素对 Ｐ Ｂ Ｍ Ｃ 的增殖反应均有显著的影响（ Ｐ＜ ００５） , Ｐ Ｂ Ｍ Ｃ 增殖反应的 Ｍ Ｔ Ｔ 比色法的最佳反应条件组合为 Ｐ Ｂ Ｍ Ｃ 浓度为１ ×１０６／ｍｌ, 植物血凝素 Ｐ Ｈ Ａ 的浓度为１２５μｇ／ ｍｌ, 细胞培养时间为２４ 小时; 影响增殖反应的先后顺序为 Ｐ Ｂ Ｍ Ｃ 浓度、 Ｐ Ｈ Ａｐ 浓度及细胞培养时间。同时确定了最有利于 Ｐ Ｂ Ｍ Ｃ 增殖的犊牛血清浓度为１０ ％ 。     

Regulation of fibroblast growth factor 9 on the differentiation of goat intramuscular adipocytes

It has been found that fibroblast growth factor receptor (FGF-FGFR) signaling can regulate the expression of adipocyte differentiation genes. FGF9 is one of the members of FGFs that mainly binds receptors FGFR2 and FGFR3. FGF9 is highly expressed in the adipose tissue of humans and mice, but there are few reports on the role of FGF9 in goat intramuscular adipocyte differentiation. Therefore, this study explored the effect of FGF9 on adipocyte differentiation through cell culture, interference, and overexpression. The expression of receptors FGFR1–FGFR4 in adipocyte differentiation and their effects on differentiation were detected to screen receptor gene of FGF9. Finally, the interaction between FGF9 and the receptor was tested by cotransfection. Our results showed that FGF9 interacts with FGFR2 to inhibit goat intramuscular adipocyte differentiation by regulating peroxisome proliferator-activated receptor gamma (PPARγ) and preadipocyte factor 1 (Pref1), which is a data support for subsequent pathway research.