Further characterization of dandelion yellow mosaic virus from lettuce and dandelion

A damaging virus isolated in the Netherlands from lettuce was studied and compared with a virus isolated from dandelion orginating from Czechoslovakia. It was found to biologically resemble dandelion yellow mosaic virus incompletely described from dandelion and lettuce in Great Britain (Kassanis, 1944, 1947) and from dandelion in Germany (Hein, 1963). Mechanical transmission was greatly improved by buffer solution and transmission byMyzus persicae seemed to be in the non-persistent manner. Longevity in vitro of the virus hardly exceeded one day. Thermal inactivation was between 60 and 65 °C and the dilution end-point was between 10 000 and 100 000. It was still infectious in leaf material dried and stored over CaCl₂ at 4 °C for 6 1/2 years. The virus was isolated and purified with difficulty and was found to consist of one type of spherical particle of ca 30 nm diameter, with a sedimentation coefficient of 159 S, a buoyant density of 1.42 g.cm^?3 and an A₂₆₀/A₂₈₀ ratio of 1.67. An antiserum was prepared with a titre of 256 in the agar double-diffusion test. The virus could be identified in crude extracts from lettuce andChenopodium amaranticolor by enzyme-linked immunosorbent assay (ELISA), but not by agar double diffusion. It could only be visualized in crude sap in the electron microscope after trapping of virus particles on antiserum-coated grids. The virus cannot yet be assigned to any known virus group. It is of potential economic importance to lettuce because of its occurrence in widely differing regions in Europe, its aggressiveness and virulence on 22 out of 23 lettuce cultivars tested (and on endive) and its pathogenicity toLactuca genotypes which are resistant to lettuce mosaic virus and other important pathogens of lettuce. ‘Laibacher Eis’ was the only cultivar showing some tolerance.     

THE BASIS OF THE DIFFERENTIAL PHYTOTOXICITY OF 4-HYDROXY-3,5-DI-IODOBENZONITRlLE*

Summary. Entry of ioxynil-¹⁴C into portions of leaves was greater with mustard than with barley or pea and was unrelated to stomatal density. Measurement of ioxynil content of sprayed plants showed that by increasing the concentration of ioxynil and adding a surfactant, almost as much ioxynil could be made to enter barley as entered mustard from a lower concentration without surfactant. Auto radiographs showed that a limited amount of ¹⁴C was translocated to a small extent in plants following localized application of ioxynil-¹⁴C. An experiment comparing leaf removal by cutting with destruction of equivalent leaf areas by ioxynil treatment suggested a greater translocated effect of ioxynil with mustard than with pea or barley. Les principes de la phytotoxicité différentielle du 4-hydroxy-3,5-di-iodobetZonitrile II. Absorption et migration     

ADSORPTION AND DESORPTION OF SIMAZINE BY SOME ROTHAMSTED SOILS

Summary. The adsorption of simazine from, and subsequent desorption into, 0–01 M calcium chloride solution was investigated using twenty-three Rothamsted soil samples from sites differing greatly in cropping history and manurial treatment. Organic carbon content was the only factor related to the ability of the soils to sorb simazine; this accounted for 90% or more of the variation between soils Equilibrium was attained during adsorption in from fewer than 2 to more than 24 hr. Equilibrium during desorption was only occasionally attained within 24 hr. Differences between theoretically predicted and measured concentrations of simazine in solution following desorption were least for soils that attained equilibrium fastest during adsorption. Differences in adsorption and desorption kinetics between soils could not be related to soil pH, organic carbon content or cropping and manuring history. Comparisons of unlimed and limed soils suggested that no simnazine was lost by acid hydrolysis during the experiments.
Adsorption et désorption de la simazine par quelques sols de Rothamsted     

EFFECT OF PARAQUAT ON MICROBIAL ACTIVITIES IN SOILS*

Summary. When paraquat was added to four different soils, nitrification was not appreciably affected but ammonification of soil organic-matter nitrogen was slightly retarded. Carbon dioxide evolution as well as oxygen consumption was used as an index of metabolic activity of soil micro-organisms and paraquat had a stimulatory effect at higher concentrations. Oxidation of added sulphur was slightly depressed. Paraquat decreased both the total mould and bacterial populations in Chehalis silt loam. After 30 days incubation the percentages of Streptomyces and Penicillia were markedly increased by most treatments but were little affected in Chehalis silt clay and Woodburn soils. Except for the temporary suppression of nitrification, paraquat had no significant influence on general microbial activities of importance to soil fertility. Some bimodal effects or toxicity inversions were observed with intermediate concentrations. Effet du paraquat sur les activités microbiennes dans les sols