覆土厚度对油松出苗的影响

为了解不同覆土厚度对油松出苗率和出苗指数的影响,进行了油松育苗随机区组试验。结果表明:当播种深度为0.5cm时,出苗率和出苗指数最高;随着覆土厚度的增大,幼苗出苗率也相应降低,当覆土厚度超过2.5cm时,几乎不能出苗。建议生产中油松播种覆土厚度保持在0.5~1.0cm。     

火烧对不同植被恢复类型物种多样性的影响

以山西省三交林场火烧迹地为研究对象,采用群落调查方法,分析了在自然更新下的不同植被恢复类型(油松人工林、灌木林、草本丛)下物种的组成及生物多样性。结果表明:(1)与对照相比,重度火烧后,草本丛的优势种重要值变化不明显,但喜阴耐旱的植物重要值显著升高;而灌木林主要以阳生耐旱草本植物占主要优势;油松人工林林分群落结构变化只是在灌木层和草本层中一些物种的更替。(2)重度火烧后所有植物的Simpson多样性指数均大于对照样地,油松人工林在重度火烧后多样性明显升高,油松人工林草本丛灌木林地。说明重度火烧2a后,不同类型植被恢复仍有一定的变化。     

针对红锥速生丰产造林技术探讨

红锥作为一种重要的造林树种,其种植受到人们的广泛重视.提升红锥的速生丰产造林技术水平,对于农民的增产增收具有重要的作用.笔者采取实验的方式,对红锥速生丰产造林技术进行了研究.     

福建柏自然资源调查研究及建议

调查了福建省内外的福建柏自然资源,分析了福建柏自然资源的分布情况及其现状,并针对保护工作提出了一些对策及建议。     

简述方正林业局第五经理期企业经营状况

经过走访调研,对黑龙江省森工林区方正林业局在第五经理期企业的经营状况进行了简要归纳和介绍,旨在为该局今后经济社会发展提供借鉴和帮助。     

轮圈式链轮设计理论的研究

本文提出链锯链轮与锯链正确啮合条件的新理论。对于小节距型号的链锯锯链确定了不同的几何参数作为原始数据 ,并在正确啮合条件下建立了一套适用于不同形式小节距锯链的轮圈式链轮设计理论公式。     

Event-specific quantitative detection of nine genetically modified maizes using one novel standard reference molecule

With the development of genetically modified organism (GMO) detection techniques, the Polymerase Chain Reaction (PCR) technique has been the mainstay for GMO detection, and real-time PCR is the most effective and important method for GMO quantification. An event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity. This study establishes the event-specific detection methods for TC1507 and CBH351 maizes. In addition, the event-specific TaqMan real-time PCR detection methods for another seven GM maize events (Bt11, Bt176, GA21, MON810, MON863, NK603, and T25) were systematically optimized and developed. In these PCR assays, the fluorescent quencher, TAMRA, was dyed on the T-base of the probe at the internal position to improve the intensity of the fluorescent signal. To overcome the difficulties in obtaining the certified reference materials of these GM maizes, one novel standard reference molecule containing all nine specific integration junction sequences of these GM maizes and the maize endogenous reference gene, zSSIIb, was constructed and used for quantitative analysis. The limits of detection of these methods were 20 copies for these different GM maizes, the limits of quantitation were about 20 copies, and the dynamic ranges for quantification were from 0.05 to 100% in 100 ng of DNA template. Furthermore, nine groups of the mixed maize samples of these nine GM maize events were quantitatively analyzed to evaluate the accuracy and precision. The accuracy expressed as bias varied from 0.67 to 28.00% for the nine tested groups of GM maize samples, and the precision expressed as relative standard deviations was from 0.83 to 26.20%. All of these indicated that the established event-specific real-time PCR detection systems and the reference molecule in this study are suitable for the identification and quantification of these GM maizes.     

Improvement of texture properties and flavor of frozen dough by carrot (Daucus carota) antifreeze protein supplementation

The effects of concentrated carrot protein (CCP) containing 15.4% (w/w) carrot (Daucus carota) antifreeze protein on texture properties of frozen dough and volatile compounds of crumb were studied. CCP supplementation lowered the freezable water content of the dough, resulting in some beneficial effects including holding loaf volume steadily and making the dough softer and steadier during frozen storage. Furthermore, SPME-GC-MS analysis showed CCP supplementation did not give any negative influences on volatile compounds of crumb and gave a pleasant aroma felt like Michelia alba DC from trans-caryophyllene simultaneously. Combining our previous results that CCP supplementation improves the fermentation capacity of the frozen dough, CCP could be used as a beneficial additive for frozen dough processing.     

Flumorph Is a Novel Fungicide That Disrupts Microfilament Organization in Phytophthora melonis

ABSTRACT The mechanism of the effects of flumorph (a novel fungicide) was investigated by analyzing alterations of hyphal morphology, cell wall deposition patterns, F-actin organization, and other organelles in Phytophthora melonis. Calcofluor white staining suggested that flumorph did not inhibit the synthesis of cell wall materials, but disturbed the polar deposition of newly synthesized cell wall materials during cystospore germination and hyphal growth. After exposure to flumorph, zoospores were able to switch into cystospores accompanied with the formation of a cell wall, whereas cystospores failed to induce the isotropic-polar switch and did not produce germ tubes but continued the isotropic growth phase. In flumorph-treated hyphae, the most characteristic change was the development of periodic swelling ("beaded" morphology) and the disruption of tip growth. Newly synthesized cell wall materials were deposited uniformly throughout the diffuse expanded region of hyphae, in contrast to their normal polarized patterns of deposition. These alterations were the result of F-actin disruption, identified with the fluorescein isothiocynate (FITC)-phalloidin staining. The disruption of F-actin also was accompanied by disorganized organelles: each swelling of subapical hyphae was associated with a nucleus. Vesicles did not undergo polarized secretion to the apical hyphae, but diffused around nuclei for the subapical growth; thus, the cell wall was thickened with periodic expansion along the hyphae. Upon removing flumorph, normal tip growth and organized F-actin were observed again. These data, as well as data published earlier, suggest that flumorph may be involved in the impairment of cell polar growth through directly or indirectly disrupting the organization of F-actin. The primary site of action by flumorph in the disruption of the F-actin organization is under investigation.     

Rapid evolution of Ophraella communa cold tolerance in new low-temperature environments

Low winter temperatures severely stress newly arriving insect species. Adaptive evolutionary changes in cold tolerance can facilitate their establishment in new environments. Ambrosia artemisiifolia, a noxious invasive plant, occurs throughout China. Ophraella communa, a biological control agent of A. artemisiifolia, mainly occurs in southern China. However, in 2012, it established populations in Beijing (39.98°N, 115.97°E) following introduction from Laibin (23.62°N, 109.37°E), implying cold adaptation. The mechanisms underlying its rapid evolution of cold tolerance remain unknown. We investigated the levels of cryoprotectants and energy reserves in adult O. communa from two latitudes. In high-latitude insects, we found high trehalose, proline, glycerol, total sugar, and lipid levels; five potential genes (Tret1a, Tret1b, Tret1-2, P5CS, and GST), responsible for regulating cold tolerance and involved in trehalose transport, proline biosynthesis, and glutathione S-transferase activation, were highly expressed. These hybridisation changes could facilitate cold temperature adaptation. We demonstrate the genetic basis underlying rapid adaptation of cold tolerance in O. communa, explaining its extension to higher latitudes. Thus, specialist herbivores can follow host plants by adapting to new temperature environments via rapid genetic evolution.