Designing a Score-Based Method for the Evaluation of the Nutritional Quality of the Gluten-Free Bakery Products and their Gluten-Containing Counterparts

Gluten-free (GF) products are consumed both by individuals with celiac disease and by an increasing number of people with no specific medical needs. Although the technological quality of GF products has been recently improved, their nutritional quality is still scarcely addressed. Moreover, the few published studies report conflicting results, mostly because the information from product nutrition facts is the only considered factor. The aim of the present study was to develop a score-based method for the nutritional evaluation of 134 packaged Italian GF bakery products and to compare it with that of 162 matched gluten-containing (GC) food items. The score included the information from the nutrition facts and the presence/absence of some nutritionally relevant components in the ingredients list. Results indicated an overall low nutritional quality of the considered GF bakery products. Additionally, with the sole exception of GF bread substitutes, there was no difference in nutritional quality between GF and equivalent GC bakery products. Future research and development of GF bakery products may take advantage of this scoring method, as it may represent an easy approach to evaluate their nutritional quality. The present findings do not justify the consumption of packaged GF bakery products by people without any specific medical needs.     

Suspected venous air embolism in three horses

Venous air embolism was suspected in 3 hospitalised horses on the basis of an inadvertently open jugular venous line. Subsequently the 3 horses developed clinical signs including cardiovascular collapse, cardiac dysrhythmia, pulmonary oedema, behavioural abnormalities and neurological signs consistent with central nervous system injury. Treatment included intranasal oxygen therapy, i.v. fluid administration and anti‐inflammatory therapy. Specific therapy aimed at treating air embolism in man is reviewed, with pertinent therapy potentially applicable to horses emphasised.     

Note: Molecular identification of ‘Candidatus phytoplasma asteris’ inducing histological anomalies inSilene nicaeensis

Numerous plants ofSilene nicaeensis having symptoms resembling those associated with the presence of phytoplasmas were observed in an extensive coastal area in the south of Italy. Microscopic observation showed histological abnormalities in the organization of tissues in symptomatic plants, and molecular tests, including PCR/RFLP analyses and nucleic acid sequencing, revealed the presence of phytoplasmas belonging to the aster yellows group (‘Candidatus phytoplasma asteris’). This is the first report of phytoplasma infection inS. nicaeensis, a wild species that colonizes the Calabrian coast. http://www.phytoparasitica.org posting June 12, 2008     

Direct measurement of the total antioxidant capacity of cereal products

A simple and rapid procedure was developed for the direct measurement of the antioxidant capacity of cereals. It entails grinding of cereals, mixing with 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) reagent, centrifugation and measure of the absorbance. The ABTS reagent was dissolved in a mixture of ethanol:water (50:50, v/v), instead of 100% ethanol, in order to overcome low solubility of water-soluble antioxidant compounds of some cereals. A reaction time of 30 min allowed plateau values to be reached during the antioxidant capacity measurement of cereal samples. The accuracy of the direct procedure was confirmed by measuring, in solid state, the antioxidant activity of pure phenolic compounds.The direct procedure gave results of total antioxidant capacities significantly higher than those determined by the traditional procedure (multiple extraction followed by alkaline hydrolysis) for most whole meal cereals, suggesting that such a procedure was not always sufficient to properly assess the antioxidant capacity of bound phenolic compounds in cereals. The proposed extraction-independent procedure for measuring antioxidant capacity of cereals will facilitate the inter-laboratory data comparison, the construction of reliable antioxidant capacity database and the screening of large sampling of cereals for their nutraceutical characteristics.     

Effects on some clay soil qualities following the passage of rubber-tracked and wheeled tractors in central Italy

There is increased use of rubber-tracked tractors for ploughing on clay soil (Vertic Cambisol) in central, south and insular Italy instead of metal-tracked tractors, because they allow travel on public roads. Field tests were carried out on arable soil previously ploughed and harrowed to compare two types of tractors, one rubber-tracked (CAT Challenger Ch 45) and one wheeled (New Holland 8770) in order to establish the compacting effects resulting from 1 and 4 passes of the tractors in the same track. The following parameters were studied: soil penetration resistance, bulk density and its increment ratio, soil shear strength, soil macroporosity and hydraulic conductivity. Multiple passes made by the two tractors induced very similar effects on the soil in regards to soil penetration resistance. Mean values of penetration resistance (0–0.20 m depth) were 1.15 MPa for the rubber-tracked tractor and 1.11 MPa for the wheeled tractor; mean values of penetration resistance (0.21–0.40 m depth) were 1.07 MPa for the rubber-tracked tractor and 1.17 MPa for the wheeled tractor. The decrease in macroporosity, in particular that of elongated pores in the soil surface layer (0–0.10 m depth) was greater in treatments involving the rubber-tracked tractor (from 20.2 to 2.7%) than for the wheeled tractor (from 20.2 to 10.3%). Following traffic of the two tractors, hydraulic conductivity decreased and the following values were found for the five treatments: control, 18.48 mm h⁻¹; wheeled tractor 1 and 4 passes, 11.15 and 7.45 mm h⁻¹, respectively; rubber-tracked tractor 1 and 4 passes, 3.25 and 1.1 mm h⁻¹, respectively. Highly significant correlations between shear strength and dry bulk density, and between hydraulic conductivity and elongated pores and total macroporosity were found. Significant linear relationships between macroporosity and penetration resistance for 1 and 4 passes of both tractors were found in the soil layers (0–0.10 m). A significant difference was found between tractors and for correlations of penetration resistance values above control values. However, in the soil layer (0–0.20 m depth), with respect to the higher degree of macroporosity and low values of penetration resistance, treatments involving wheeled tractor (1 pass) showed a lower degree of soil compaction than was observed after 1 pass of the rubber-tracked tractor.     

The effect of urban refuse compost and different tractors tyres on soil physical properties, soil erosion and maize yield

Application of urban refuse compost to agricultural soil could help to solve municipalities' problems related to the increasing production of waste only if soil property improvement and environmental conservation can be demonstrated. The use of low-pressure tractor tyres is another proposal in modern agriculture for reducing soil compaction. This study thus aimed to detect the effects of both compost and low-pressure tractor tyres on soil loss, runoff, aggregate stability, bulk density, penetrometer resistance and maize (Zea mays L.) yield. A 3-year field experiment was carried out on a hilly (15% slope) clay loam soil in central Italy. Twelve plots (200 m² each) were monitored with tipping-pot devices for runoff and soil erosion measurement. Treatments were: compost addition (64 Mg ha⁻¹), mineral fertilisation, use of low-pressure tyres, use of traditional tyres, with three replicates, in a fully randomised block design. Compost was applied once at the beginning of the experiment. Runoff reduction due to compost ranged between 7 and 399 m³ ha⁻¹ during seasons, while soil erosion was reduced between 0.2 and 2.4 Mg ha⁻¹. Mean weight diameter (MWD) of stable aggregates, measured on wheel tracks, increased by 2.19 mm, then progressively decreased. Compost significantly increased bulk density by 0.08 Mg m⁻³ due to its inert fraction content. This effect was less evident in the second and third year, probably due to harrowing. Maize yields were slightly, but significantly, reduced in composted plots by 1.72 Mg ha⁻¹ in the third year. Low-pressure tyres significantly reduced soil loss in the third year by 1 Mg ha⁻¹. Furthermore, they did not significantly influence runoff volumes and soil structural stability. Low-pressure tyres or compost addition were singly able to prevent an increase in penetrometer resistance due to agricultural machinery traffic. Low-pressure tyres increased the maize yield during the 3 years and the difference (0.4 Mg ha⁻¹) became significant in the third year. In conclusion, results show the positive lasting effect of compost in ameliorating soil physical properties and reducing runoff and soil erosion. Low-pressure tyres appear justifiable both for the observed increase of grain production and reduction of soil compaction. This latter effect is, nevertheless, masked by compost addition which is also able to reduce penetrometer resistance. Further research is required to explain the causes of the slight inhibition of grain yield observed when compost was compared with mineral fertilisation.     

PSII photochemistry is the primary target of oxidative stress imposed by ozone in Tilia americana

Trees are essential in the urban environment not only because of their aesthetic and social values, but also for their effects on air quality. Data of the present experiment show some of the integrated mechanisms that may confer sensitivity/tolerance in Tilia americana saplings exposed to ozone treatment (120 ppb, 5 h day^?1 for 45 consecutive days) in order to improve the management of green spaces responding to oxidative stress. At the end of exposure, plants do not exhibit any foliar symptoms. Profiles related to membrane injury, photosynthetic gas exchange, chlorophyll a fluorescence, pigment content and enzymes/metabolites linked to the synthesis of phenylpropanoids show a vulnerability in terms of: (i) change in the ionic and solute reactions of the membrane cells (maximum value of +34%, 28 days after the beginning of fumigation, compared to controls); (ii) inhibition of the carbon assimilation (?41%), mostly as a consequence of non-stomatal limitation of assimilation rate; (iii) progressive inhibition of the Calvin cycle, as demonstrated by decreases in Rubisco carboxylation efficiency and regeneration capacity (?35 and ?21%, respectively, at the end of exposure), quantum yield of electron transfer at PSII and in the fraction of energy passively dissipated as heat and fluorescence (?34% and ?31%); (iv) senescence process (decline in demand for reducing power and energy at the end of treatment); (v) damage to the chlorophyll pigment system. However, the activation of xanthophylls cycle and phenylpropanoid metabolism, that can be considered a strategy in plants in order to regulate light absorbed energy and to scavenge reactive oxygen species does not preserve PSII photochemistry from impairment at the end of exposure.     

Sustainable sediment management in coastal infrastructures through an innovative technology: preliminary results of the MARINAPLAN PLUS LIFE project

Journal of Soils and Sediments - The paper aims to show the preliminary monitoring and field test results of the innovative technology tested in the framework of the MARINAPLAN PLUS LIFE project...