Metabolite Profiling of Rosemary Cell Lines with Antiproliferative Potential against Human HT-29 Colon Cancer Cells

Plant Foods for Human Nutrition - Rosemary (Rosmarinus officinalis) is a culinary and medicinal plant used in food and pharmaceutical industry. The wide range of biological activities is mainly...     

Factors involved in alleviating water stress by partial crop removal in pear trees

We studied the relief of water stress associated with fruit thinning in pear (Pyrus communis L.) trees during drought to determine what mechanisms, other than stomatal adjustment, were involved. Combinations of control irrigation (equal to crop water use less effective rainfall) and deficit irrigation (equal to 20% of control irrigation), fruit load (unthinned and thinned to 40 fruits per tree) and root pruning (pruned and unpruned) treatments were applied to pear (cv. 'Conference') trees during Stage II of fruit development. Daily patterns of midday stem water potential (Psi(stem)) and leaf conductance to water vapor (g(l)) of deficit-irrigated trees differed after fruit thinning. In response to fruit thinning, gl progressively declined with water stress until 30 days after fruit thinning and then leveled off, whereas the effects of decreased fruit load on Psi(stem) peaked 30-40 days after fruit thinning and then tended to decline. Soil water depletion was significantly correlated with fruit load during drought. Our results indicate that stomatal adjustment and the resulting soil water conservation were the factors determining the Psi(stem) response to fruit thinning. However, these factors could not explain differences in daily patterns between g(l) and Psi(stem) after fruit thinning. In all cases, effects of root pruning treatments on Psi(stem) in deficit-irrigated trees were transitory (Psi(stem) recovered from root pruning in less than 30 days), but the recovery of Psi(stem) after root pruning was faster in trees with low fruit loads. This behavior is compatible with the concept that the water balance (reflected by Psi(stem) values) was better in trees with low fruit loads compared with unthinned trees, perhaps because more carbon was available for root growth. Thus, a root growth component is hypothesized as a mechanism to explain the bimodal Psi(stem) response to fruit thinning during drought.     

Response of winter root starch concentration to severe water stress and fruit load and its subsequent effects on early peach fruit development

Effect of water stress during stage III of peach fruit development on winter root starch concentration (RSC) and subsequent reproductive development was studied. Two irrigation treatments were applied in two consecutive seasons (2003-2004): full irrigation (FI) and no irrigation during stage III of fruit development until visible leaf wilting (LWI), which occurred when midday stem water potential reached -1.80 MPa. Three fruit thinning intensities were applied within each irrigation treatment. The year 2005 was a recovery year in which all trees received full irrigation and commercial fruit thinning. Water deficit and high fruit loads in the previous season significantly reduced the concentration of winter RSC. Fruit set and fruit growth from full bloom to 30 days after full bloom (30 DAFB) increased with increasing winter RSC before other factors, such as inter-fruit competition and availability of carbon from current photosynthesis, came into play. Consequently, severe water stress reduced the total number of fruits and fruit dry mass growth 30 DAFB. However, during the recovery year and after fruit thinning, fruit loads were similar between irrigation treatments and yield capacity remained unaffected. Peach fruit production recovered quickly from the deleterious effects of two consecutive years of water stress because of a combination of two factors: (1) reduced initial fruit set that was still adequate to achieve a commercial crop; and (2) the low sensitivity of fruit growth 30 DAFB to winter RSC.     

Detection and characterization of Shiga toxin-producing Escherichia coli in captive non-domestic mammals

Shiga toxin producing-Escherichia coli (STEC) is an important emerging pathogen, and ruminants are recognized as their main natural reservoir. The aim of this work was to establish the frequency of STEC in non-domestic mammals of the Zoo and Botanical Garden of La Plata City, Argentina, and to pheno-genotypically characterize STEC isolates. By polymerase chain reaction (PCR), Shiga toxin (stx) gene sequences were detected in 50.8% of 65 fecal samples. Twenty-five STEC strains were isolated from 38.5% of the Zoo's animals. Ten species of order Cetartiodactyla and one species of order Rodentia were recognized as new STEC carriers. STEC strains belonged to 7 different serotypes including new serotypes O12:H25 and O13:H6. Serotype O146:H28, previously associated with human infections, represented 24% of STEC isolates. The most frequent Shiga toxin identified were type 1c and type 2c. Nineteen strains were positive for iha gene, 8 strains were positive for ehxA gene. Moreover, all strains were positive for lpfAO113 and negative for rfbO157, eae, saa, lpfAO157/OI-141, lpfAO157/OI-154, efa1, and toxB genes. Results obtained by XbaI-pulsed-field gel electrophoresis (XbaI-PFGE) confirmed the transmission of STEC strains among different animal species and suborders. In addition, we observed a potential association between STEC-harboring animal and factors such as belonging to order Cetartiodactyla, living in a pit, and belonging to a non-autochthonous species. This is the first work developed with zoological mammals and STEC in Argentina.     

Efficacy of metalaxyl for blue mould control and its persistence on tobacco

Foliar sprays of metalaxyl, benalaxyl, and cymoxanil plus mancozeb gave better control of blue mould (Peronospora tabacina) than mancozeb alone applied as a spray, or metalaxyl applied to the soil. Before flue-curing, the mean values of metalaxyl residues in tobacco leaves were significantly higher from foliar spray treatments (5.09 μg g⁻¹), than from soil treatments (0.93 μg g⁻¹). Residues had decreased after flue-curing (foliar spray treatment 2.51 μg g⁻¹; soil treatment 0.69 μg g⁻¹), especially on samples taken in September. Curing considerably reduced metalaxyl residues in all cases. Residues of mancozeb ranged from 59.5 to 224.2 μg g⁻¹ before flue-curing and from 11.0 to 22.1 μg g⁻¹ after flue-curing. All these residues were calculated on a dry weight basis. Imidazolidine-2-thione (ethylenethiourea) residues from mancozeb were always below the sensitivity limit of the method used.     

Aberrant right subclavian artery causing megaoesophagus in three cats

Three entire, domestic, shorthair male cats (age range: 3 months to 5 years) were referred because of regurgitation. Megaoesophagus attributable to aberrant right subclavian artery, originating from the aorta at the level of the fourth intercostal space, was diagnosed in all cats using thoracic radiography and CT angiography. One cat had concurrent patent ductus arteriosus with a normal aortic arch. Three‐dimensional volume‐rendered CT images were used to assess the malformations and to plan surgery for the treatment of the vascular anomalies. Different surgical approaches were used in the two kittens. The third cat was not operated. CT angiography is well suited for preoperative planning in cats with aberrant right subclavian artery alone or in combination with other vascular anomalies.     

Effect of left vs. right recumbency on the vertebral heart score in normal dogs

The effect of right vs. left recumbency on computation of the vertebral heart score (VHS) was assessed in 63 healthy dogs. The VHS was significantly higher in right lateral recumbency (9.8 +/- 0.6 vs. 9.5 +/- 0.8; P < 0.0004). Gender and dog size did not significantly influence VHS values while there was m oredifference between left and right side measurements when considering the type of thorax (P = 0.055).     

Effect of lactating sows’ diet supplemented with cactus (Opuntia ficus-indica) on feed intake and reproductive and productive post-weaning performances

Tropical Animal Health and Production - The effect of cactus (Opuntia ficus-indica) added to the diet of lactating (21&nbsp;days of lactation) sows on voluntary feed intake, and its impact on...     

Bio-guided isolation of the cytotoxic corytenchine and isocoreximine from roots of Annona cherimolia

The roots of Annona cherimolia have been shown to contain the known tetrahydroprotoberberine alkaloids corytenchine (1) and isocoreximine (2), investigated for their cytotoxic properties.