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81.
OBJECTIVE: To determine whether or not aquabirnavirus is present in Victorian fish hatcheries. PROCEDURE: Milt and ovarian fluids were collected from brood stock at 12 hatcheries and cultured in two sensitive cell lines for the presence of viruses. RESULTS: No cytopathic effect was detected indicating the absence of virus. CONCLUSION: There is no evidence of infection with aquabirnavirus or Epizootic Haematopoietic Necrosis virus in Victorian fish hatcheries. 相似文献
82.
Cryptosporidia on dairy farms and the role these farms may have in contaminating surface water supplies in the northeastern United States 总被引:12,自引:0,他引:12
The prevalence and risk factors for shedding of cryptosporidia by dairy cattle and calves and the prevalence and risk factors for cryptosporidia in surface waters associated with dairy farms were determined for a well-defined watershed in the northeastern United States. Eleven dairy farms were enrolled in the study and subjected to monthly sampling over a 6-month period. Animal-, water-, and manure-management practices were determined by survey and fecal, on-farm water, and stream samples were obtained monthly and evaluated for the presence of cryptosporidia. Ninety-one percent of the dairy farms in our study had Cryptosporidium on their premises. Fifteen percent of the sampled calves 0–3 weeks of age were shedding cryptosporidia. The risk factors for calves shedding cryptosporidia were contact between calves and frequent bedding changes. The probability of shedding cryptosporidia decreased with age. Nine percent of farm-associated stream samples were cryptosporidia-positive. The single risk factor for detecting cryptosporidia in surface water was increasing frequency of spreading of manure on fields. The probability of detecting cryptosporidia in streams decreased as 5-day cumulative precipitation increased. There were no animal-associated or barnyard-management features associated with detecting cryptosporidia in farm-impacted streams. 相似文献
83.
Caldin M Furlanello T Lubas G 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2000,29(2):51-54
Abstract: D-dimer is a neoantigen formed when thrombin initiates the transformation of fibrinogen to fibrin; it is derived from plasmin digestion of cross-linked fibrin. In human medicine, the usefulness of this analyte in diagnosing disseminated intravascular coagulation (DIC) has been assessed in patients fulfilling the clinical and laboratory requirements for this disorder. In canine medicine, the use of D-dimer is relatively new. Detailed studies are needed to understand the relationship between D-dimer concentration in plasma and DIC status in dogs. We validated a D-dimer immunoturbidimetric assay (Tina-quant [a] D-Dimer, Boehringer Mannheim) in canine citrated plasma samples. Intra-assay and interassay variability (coefficient of variation) was 5.63% and 8.82%, respectively. The assay was linear, using 2 samples with low and high D-dimer concentrations (r = .996 and .998). Accuracy was 102.2% and 95.7% based on a recovery study in which 2 samples were assessed. Reference values for D-dimer were established using 70 healthy dogs that were assessed clinically and evaluated on the basis of a complete laboratory workup. The reference range was set between 0.02 and 0.28 μg/mL (chi-square test for normal distribution, P > .05). 相似文献
84.
Ranchers in Venezuela historically have controlled the cattle-fever tick, Boophilus microplus (Canestrini), with acaricide treatments of cattle but no technical planning. We developed a simulation model to evaluate cattle-tick population dynamics in systematic pasture rotation systems and Integrated Pest Management (IPM) approaches to managing ticks in the tropical dry-forest ecological zone of Venezuela. Model output showed five generations of cattle-ticks produced each year throughout the dry and rainy seasons that occur in this zone. Sensitivity analyses showed disproportionately large changes in on-host B. microplus populations in response to small changes in larval mortality rates, such as those resulting from differences in the innate resistance of cattle to tick parasitism. Simulation results with 1-6 pasture systems suggest that adjusting the graze:rest sequence with systematic rotation among 4-6 pastures could suppress, but not eradicate, tick populations. 相似文献
85.
Application and field validation of a PCR assay for the detection of Mycoplasma hyopneumoniae from swine lung tissue samples. 总被引:1,自引:0,他引:1
Hugh Y Cai Tony van Dreumel Beverly McEwen Geoff Hornby Patricia Bell-Rogers Pat McRaild Gaylan Josephson Grant Maxie 《Journal of veterinary diagnostic investigation》2007,19(1):91-95
A PCR assay was validated for the detection of Mycoplasma hyopneumoniae in porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae. In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniae infection by M. hyopneumoniae PCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test-negative cases had lesions compatible with M. hyopneumoniae infection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively. 相似文献
86.
The objectives of this study were to characterize the glycemic and insulinemic responses of Thoroughbred broodmares fed late spring pasture only or a mixture of pasture and a high starch or low starch feed and to test hypotheses about differences in the glycemic and insulinemic effects of these dietary regimes. A group of 15 mares were divided into three treatment groups; pasture and high starch feed (PHS), pasture and low starch feed (PLS), and pasture only (PO) and maintained in these groups for 4.5 months prior to this study. These groups were maintained on a single pasture that was temporarily divided into three sections. The study protocol was conducted over two days. On day 1 the mares were fed their respective treatments and on day 2 all mares were allowed access to pasture only. On both days plasma glucose and insulin were measured in samples taken over a 7.5 h period. Baseline measurements for glucose and insulin were not different between any of the treatment groups on either day (P > 0.05). The baseline insulin concentrations of these pasture-kept mares (26.7 ± 8.3 mIU/L) were high compared to those reported from stall-kept horses. Plasma glucose and insulin on day 1 were influenced by treatment group, sample time, and an interaction between treatment and time (P < 0.05). On day 2 there was no significant influence of treatment group (P > 0.05). Glucose and insulin rose to higher (P < 0.01) peak concentrations in the PHS group on day 1 when compared to the PLS and PO groups, with no difference (P > 0.05) detected between the PLS and PO groups. These results are reflected in greater areas under the concentration-time curves for glucose and insulin in the PHS group on day 1 (P < 0.05). On day 2 there were no differences in any of the glucose and insulin characteristics for any of the treatment groups (P > 0.05). These results indicate a clear difference in the glycemic and insulinemic effect of the PHS feed compared to the PLS and PO groups. Of further interest are the glucose and insulin characteristics of these pasture-kept mares that indicate a low insulin sensitivity and high insulin secretory response. This study provides further information on factors influencing glycemic and insulinemic responses in horses. 相似文献
87.
The effects of field aging (0–28 days) and pheromone loading rate on the longevity of red rubber septa loaded with the sex
pheromone blend of the oriental fruit mothGrapholita molesta (Busck), were evaluated in North Carolina apple orchards in 2002. Separate field tests examined the influence of trap height
and pheromone loading rate of rubber septa on trap catches of adultG. molesta males in an abandoned orchard. The loss of the major pheromone component, (Z)-8-dodecenyl acetate (Z8–12:OAc), from red rubber
septa over a 4-week period exhibited a relatively constant release rate with 30, 100 and 300 μg pheromone. Trap catch was
significantly higher in pheromone traps placed in the upper canopy than in those in the lower canopy. Pheromone traps baited
with 100μg lures caught more moths compared with those loaded with 300 μg. There was no apparent relationship between pheromone
trap catch and septa age, with trap catch appearing to be primarily a function ofG. molesta population density.
http://www.phytoparasitica.org posting May 14, 2006. 相似文献
88.
Prediction of multinomial probability of land use change using a bisection decomposition and logistic regression 总被引:1,自引:1,他引:1
Land use change is an important research area in landscape ecology and urban development. Prediction of land use change (urban
development) provides critical information for making the right policies and management plans in order to maintain and improve
ecosystem and city functions. Logistic regression is a widely used method to predict binomial probabilities of land use change
when just two responses (change and no-change) are considered. However, in practice, more than two types of change are encountered
and multinomial probabilities are therefore needed. The existing methods for predicting multinomial probabilities have limits
in building multinomial probability models and are often based on improper assumptions. This is due to the lack of proper
methodology and inadequate software. In this study, a procedure has been developed for building models to predict the multinomial
probabilities of land use change and urban development. The foundation of this procedure consists of a special bisection decomposition
system for the decomposition of multiple-class systems to bi-class systems, conditional probability inference, and logistic
regression for binomial probability models. A case study of urban development has been conducted to evaluate this procedure.
The evaluation results demonstrated that different samples and bisection decomposition systems led to very similar quality
and performance in the developed multinomial probability models, which indicates the high stability of the proposed procedure
for this case study. 相似文献
89.
This study proposes a methodology for forecasting crop yields at intermediate times in the growing season using Markov chain theory. A Markov chain is constructed, based on historical data, to provide forecast distributions of crop yield for various crop and soil moisture condition classes at selected times prior to harvest. Expected yield and the associated standard error are obtained for each condition class. The methodology is compared to a regression approach in which the independent variables are the various crop and soil moisture conditions. The Markov chain approach requires less stringent assumptions and provides more information than the regression approach. However, the potential loss of precision in the forecast using this approach requires separate evaluation for each application. A data base created by the CERES-Maize model, which simulates the growth and development of a corn crop, is used to demonstrate the development of the forecast yield distributions using the Markov chain approach. 相似文献
90.
Grady H. Shelton DVM Chris K. Grant PhD DSc Michael L. Linenberger MD Janis L. Abkowitz MD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1990,4(6):317-319
Griseofulvin administration was associated with the development of absolute neutropenia in six of seven (86%) cats with feline immunodeficiency virus (FIV) infection. The neutropenia was severe (less than 400 neutrophils/microliter) in four of the six affected cats, and one cat died from sepsis. Neutrophil counts returned to baseline values within 15 days after drug withdrawal in all surviving cats. No symptoms or hematologic abnormalities were observed in four normal (FIV-seronegative) cats treated with the same lot of griseofulvin at equivalent doses. Neutropenia recurred in two of two FIV-seropositive cats upon griseofulvin rechallenge. Cats with FIV infections appear to be at increased risk for griseofulvin-associated neutropenia. This phenomenon may be analogous to the increased frequency of antibiotic-induced neutropenias observed in humans infected with the human immunodeficiency virus. 相似文献