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51.
Qiyan Xiong Yanna Wei Zhixin Feng Yuan Gan Zhanjun Liu Maojun Liu Fangfang Bai Guoqing Shao 《Veterinary journal (London, England : 1997)》2014,199(2):268-274
An attenuated Mycoplasma hyopneumoniae vaccine that requires intrathoracic administration is commercially available for use against mycoplasmal pneumonia in China. Given the limitations of such a route of administration, this study was undertaken to assess the capacity of an ISCOM-matrix adjuvant to enhance immunogenicity following intramuscular use. Immune responses in pigs following vaccination and subsequent intra-tracheal bacterial inoculation were examined using lymphocyte proliferation, serology and mucosal IgA in both nasal and saliva swabs.Vaccination induced clear lymphocyte proliferation, but only slight serum antibody responses although these were significantly increased following experimental infection. Mucosal IgA was not detected in either nasal or salivary secretions. Following bacterial challenge, animals vaccinated with the adjuvant-containing live vaccine exhibited less severe pulmonary lesions (median score 3.67) than unvaccinated pigs (median score 13.58). The degree of ciliary loss on the respiratory tract surface was reduced in vaccinated pigs compared with experimentally infected controls. The findings indicated that the adjuvant vaccine administered IM provided protection against experimentally induced mycoplasmal pneumonia and could have commercial potential. 相似文献
52.
种植密度对不同株型青贮玉米产量及相关性状的影响 总被引:2,自引:0,他引:2
本研究以平展型玉米(Zea mays)雅玉青贮8号、半紧凑型品种京科青贮516和紧凑型品种黎民518为材料,研究4个种植密度对不同株型的青贮玉米产量及相关性状的影响。结果表明,青贮玉米鲜草产量和籽粒产量的最佳适宜密度不同,鲜草产量的适宜密度高于籽粒产量的适宜密度。随群体密度的增加玉米刈割青贮时的绿叶片数、单株鲜重、穗长、穗粗、穗行数、行粒数呈下降趋势;秃尖长、株高、穗位高、空秆率、倒伏(折)率、瘤黑粉病病株率逐渐增加;高密度下千粒重降低;青贮生育期略有延长。供试品种中黎民518最耐密植,京科青贮516边行优势强,最不耐密植。 相似文献
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详细地综述了青岛市草地资源现状,分析了开发利用的有利条件和草地畜牧业发展趋势,提出了草地开发利用对策。 相似文献
55.
本试验是对测定饲料中钙含量的3种方法,即双波长吸光光度法、MSA—DBM显色法和高锰酸钾法的比较试验。综合本实验结果,建议饲料生产中采用MSA—DBM显色法,可以简便快速的得到可靠结果。 相似文献
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57.
为评估四川省兽医系统实验室检测能力和水平,2018—2020年四川省动物疫病预防控制中心组织全省21个地市(州)级兽医实验室开展了检测能力比对。结果显示:2018—2020年全省整体平均正确率分别为95.59%、97.09%和97.88%,说明四川省地市(州)级兽医实验室检测能力处于较高水平,能满足非洲猪瘟、禽流感等重大动物疫病检测需要。同时,部分实验室也反映出一些问题,如设备差、检测水平不高、检测报告不规范等。建议加大兽医实验室建设力度,更新完善检测设施设备;加强实验室技术人员培训,提升其检测能力和水平;同时继续推进兽医系统实验室考核工作,加强比对考核。 相似文献
58.
含PRRS病毒ORF5的伪狂犬病病毒TK基因缺失转移载体的构建 总被引:2,自引:0,他引:2
提取伪狂犬病病毒(PRV)BarthaK61株基因组DNA,用限制性内切酶KpnI充分消化,回收5.9kb片段(J片段),将其克隆于质粒pUC119 KpnI位点上,获得pBKJ。用两对针对PRV TK基因的特异性引物对重组质粒进行PCR鉴定,证明其中含有PRV TK基因。然后用KpnI、PstI和BamHI等限制性内切酶对其进行酶切分析,确定了克隆片段的物理图谱。进一步研究证实TK基因位于其中的 相似文献
59.
Junzheng Du Shandian Gao Huiyun Chang Guozheng Cong Tong Lin Junjun Shao Zaixin Liu Xiangtao Liu Xuepeng Cai 《Veterinary immunology and immunopathology》2009,131(3-4):190-199
Integrins are heterodimeric adhesion receptors that participate in a variety of cell–cell and cell–extracellular matrix protein interactions. Many integrins recognize RGD sequences displayed on extracellular matrix proteins and the exposed loops of viral capsid proteins. Four members of the αv integrin family of cellular receptors, αvβ3, αvβ6, αvβ1 and αvβ8, have been identified as receptors for foot-and-mouth disease virus (FMDV) in vitro, and integrins are believed to be the receptors used to target epithelial cells in the infected animals. To analyse the roles of the αv integrins from a susceptible species as viral receptors, we have cloned Bactrian camel αv, β3 and β6 integrin cDNAs and compared them to those of other species. The coding sequences for Bactrian camel integrin αv, β3 and β6 were found to be 3165, 2289 and 2367 nucleotides in length, encoding 1054, 762 and 788 amino acids, respectively. The Bactrian camel αv, β3 and β6 subunits share many structural features with homologues of other species, including the ligand binding domain and cysteine-rich region. Phylogenetic trees and similarity analyses showed the close relationships of integrin genes from Bactrian camels, pigs and cattle, which are each susceptible to FMDV infection, that were distinct from the orders Rodentia, Primates, Perissodactyla, Carnivora, Galliformes and Xenopus. We postulate that host tropism of FMDV may in part be related to the divergence in integrin subunits among different species. 相似文献
60.
Chuan Yu Qiu Liu Aijian Qin Xuming Hu Wencai Xu Kun Qian Hongxia Shao Wenjie Jin 《Veterinary research communications》2013,37(4):277-283
Marek’s disease virus (MDV) is a highly cell-associated herpesvirus that causes a disease in chickens characterized by tumor formation and immunosuppression. The changes of major histocompatibility complex (MHC) expression in different MDV-infected cells are not completely understood. In this study, we investigated the expression of the Class I MHC and β2-microglobulin (β2m) genes in response to MDV infection at different time points by real-time PCR. In both in vitro and in vivo, the expression levels of Class I MHC and β2m genes were upregulated during early MDV infections in comparison to control cells; We also found that the expression of Class I MHC gene was downregulated in BudR (5-bromo-2′-deoxyuridine)-treated MSB1 cells at 48 h and MDV-infected chicken embryo fibroblast cells (CEF) at 120 and 168 h post infection (hpi); Furthermore, compared to control groups, Class I MHC and β2m expression levels were downregulated in peripheral blood lymphocytes (PBLC) from MDV-infected chickens at 14 and 28 days post infection (dpi); Interestingly, both Class I MHC and β2m gene expression levels increased again in PBLC from MDV RB1B-infected chickens at 35 dpi, in which MDV was in the latent or transformed infection stages. In addition, Class I MHC expression was clearly decreased in MDV-infected CEF at 120 hpi although β2m expression was significantly increased. These changes in Class I MHC and β2m gene expression might provide more insights into host-virus interaction. 相似文献