赖氨酸修饰对梯棱羊肚菌黑色素结构和理化特性的影响

为了进一步提高梯棱羊肚菌黑色素的提取率及溶解性,本试验采用单因素、Plackett-Burman试验、响应面试验对纤维素酶-超声波协同提取梯棱羊肚菌黑色素的提取工艺进行优化研究。通过赖氨酸修饰,并对修饰前后的梯棱羊肚菌黑色素进行结构表征、理化性质及稳定性研究。结果表明,在NaOH浓度为1.54 mol/L,纤维素酶添加量为20 mg/g,纤维素酶酶解时间为78.6 min,料液比为1:30,酶解温度为40 ℃,超声时间为80 min条件下提取的梯棱羊肚菌黑色素最优。未修饰的梯棱羊肚菌黑色素不溶于水,色价值为480.24,修饰后的黑色素溶解度为1 016 g/L,色价值为1 771.18,比未修饰的黑色素在溶解性、色价值方面均有所提高。此外,在不同的温度、光照、pH条件下,修饰前后的梯棱羊肚菌黑色素均比较稳定。以上研究结果为梯棱羊肚菌黑色素的高效提取及其产品的开发利用奠定了理论基础。     

糯米糍荔枝果实内源激素与落果的关系

以糯米糍荔枝生理落果期的田间调查和胚胎发育研究为基础,探讨了内源激素与生理落果的关系.结果表明,糯米糍生理落果高峰的主要原因是:第1次因授粉受精不良导致的生长促进类激素尤其是CTK水平过低;第2次与胚乳为胚的发育所利用而消失、IAA和GAs急剧降低有关;第3次与胚的败育造成CTK、GAs和IAA的全面下降有关;第4次发生在假种皮(果肉)迅速生长期,与种皮和假种皮ABA升至高水平有关.此外,还探讨了ABA /(IAA+GAs+CTK)与生理落果的相关性,以及胚囊、珠被和假种皮等果实内部组分内源激素的变化特点.     

胚培杏新品种———山农凯新1号

 ‘山农凯新1号’是以丰产性强的‘凯特’杏为母本, 以品质优良的‘新世纪’杏为父本进行有性杂交, 结合胚培技术育成的新品种, 具有成熟早、香味浓、品质优、自交坐果率高、丰产性强等特点。     

洛川县苹果套袋栽培情况调查

介绍了洛川县60个苹果园的套袋数量、果袋种类、套袋经济效益及果实的外观质量。提出了制订苹果套袋标准化操作规程,加强技术培训,确保优质果袋投放市场的建议。     

浅谈HACCP在机制针形名茶中的应用

对HACCP作简要介绍，根据HACCP体系的要求，分析了机制针形名茶过程中各个环节可能出现的危害因素，用HACCP的原理确立了关键控制点，并且建立了相应的控制措施。     

极早熟大白菜新品种豫新50的选育

采用游离小孢子培养双单倍体技术快速育成早熟大白菜杂交一代新品种豫新50。结果表明:豫新50为极早熟耐热品种,从播种到采收50-56d,高抗病毒病和软腐病,抗霜霉病;平均667m2产净菜2716.87kg,株形紧凑,适宜密植;叶球矮桩叠抱,整齐度高,商品性好;质地柔嫩,风味佳,每100g鲜样含粗蛋白质1.19g,粗纤维0.40g,维生素C20.8mg,可溶性糖2.80g。     

Effects of oxidized low density lipoprotein and lysophosphatidylcholine on cholesterol efflux from mouse peritoneal macrophage foam cells

AIM:To explore the effects of oxidized low density lipoprotein (OxLDL) and one of its component— lysophosphatidylcholine (LPC) on cholesterol efflux from mouse macrophage foam cells.METHODS:(1) Cholesterol efflux induced by apoAI from mouse peritoneal macrophage foam cells loaded with OxLDL or acylated LDL(AcLDL) was measured. (2) Cholesterol efflux induced by LPC and apoAI from macrophage foam cells separated from normal or apoE gene deficient (E⁰) mouse loaded with AcLDL were measured.RESULTS:(1) When the macrophage foam cells were incubated with apoAI, cholesterol efflux from AcLDL-induced macrophage foam cells increased significantly compared to that of OxLDL-induced macrophage foam cells. (2) LPC promoted cholesterol efflux from macrophage foam cells in relation to both dosage and time. When LPC was incubated with E⁰ mouse macrophage foam cells, the released cholesterol mass was significantly lower than that of normal mouse macrophage foam cells. It was also found that cholesterol efflux induced by apoAI normally occurred in E⁰ mouse macrophage foam cells.CONCLUSIONS:(1) OxLDL accumulated cholesterol in macrophages and impair cholesterol efflux. (2) LPC induced cholesterol efflux from macrophage foam cells, which may occur via apoE pathway.     

Study on the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes of mouse

AIM: To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes(SCDC) of mouse. METHODS: Embryonic stem cells of D₃ line(ES-D₃) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D₃ cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC. RESULTS: The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC. CONCLUSION: The functional expression of ion channels occurred during ES-D₃ cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC.     

分蘖洋葱的特性及高效栽培技术研究

分蘖洋葱最突出的特性是分蘖性强，生长快，分蘖的多少直接影响分蘖洋葱的产量。试验表明：12-20℃条件最适宜其生长，良好的土壤营养和适宜的环境有利于分蘖洋葱的分蘖生长，获得高产、优质商品。     

阿魏蘑与杏鲍菇高产优质栽培模式研究

试验证明，阿魏蘑和杏鲍菇最佳栽培模式为菌棒半脱袋竖向畦栽半覆土栽培模式；最适环境条件为菇房温度13～20℃，空气相对湿度85％～95％；最佳栽培设施为半地下冬暖式塑料大棚。