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21.
Christian Chervin Sarita Kulkarni Simone Kreidl Fiona Birrell DeAnn Glenn 《Postharvest Biology and Technology》1997,10(3):239-245
Short periods of elevated temperature under controlled atmospheres (CA) effectively control insect pests. Cold treatment is also an effective non-chemical disinfestation process. If synergistic effects can be found by combining treatments, these may provide opportunities for cost reduction. Tests were performed to evaluate the tolerance of Packham's Triumph pears (Pyrus communis L.) to a range of temperatures (30–40 °C) combined with low oxygen (O2 < 1 kPa). Treatment duration was 16–48 h and was followed by 1 month storage at 0 °C under air. When held at 30 °C, pears withstood up to 30 h of hypoxia. After cold storage, pears ripened slightly faster than controls but were undamaged. A temperature of 35 °C induced slight skin browning, and 40 °C resulted in substantial skin blackening. Some treatments were also tested on survival of lightbrown apple moth (LBAM), Epiphyas postvittana (Walker). All developmental stages were subjected to either 16 h at 30 °C, or 16 h under hypoxia, or 1 month at 0 °C, or a combination of the three treatments. With all treatments combined, all eggs, larvae and adults were killed. Only 4% of the pupae produced adults and combined treatments led to an increase in pupa mortality of 38%. A combined treatment (tolerated by pears) consisting of 30 h at 30 °C under low O2 plus 1 month cold storage under air, killed 100% of LBAM pupae, and 100% of 5th instar larvae of both codling moth, Cydia pomonella (L.), and oriental fruit moth, Grapholita molesta (Busck). Implementation of such treatments would not require substantial investments for fruit industries equipped with CA storage facilities. 相似文献
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Fry FH Okarter N Baynton-Smith C Kershaw MJ Talbot NJ Jacob C 《Journal of agricultural and food chemistry》2005,53(3):574-580
Allicin, an active ingredient of garlic, possesses a range of antimicrobial properties. Unfortunately, certain properties of the compound, such as chemical instability and low miscibility with water, have hampered its practical use in the past. Here, we show that it is possible to use a binary system consisting of the plant enzyme alliinase and its substrate alliin to generate allicin, and hence antifungal activity, in situ. During application, the two inactive components generate compounds that inhibit growth and infection-related development of the rice blast fungus Magnaporthe grisea. It is therefore possible to "trigger" biological activity in a controlled, yet effective manner. Apart from circumventing many of the drawbacks of allicin, this binary system has additional important advantages, such as low toxicity of its individual components and selective activation. Importantly, alliinase is also able to use different substrates, therefore paving the way to a range of novel, binary antimicrobial systems with custom-made chemical and biochemical properties. 相似文献
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The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-beta. We show that importin-beta is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-beta and NuMA. Importin-beta therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis. 相似文献
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Mammalian epidermis is maintained by self-renewal of stem cells, but the underlying mechanisms are unknown. Deletion of Rac1, a Rho guanosine triphosphatase, in adult mouse epidermis stimulated stem cells to divide and undergo terminal differentiation, leading to failure to maintain the interfollicular epidermis, hair follicles, and sebaceous glands. Rac1 exerts its effects in the epidermis by negatively regulating c-Myc through p21-activated kinase 2 (PAK2) phosphorylation. We conclude that a pleiotropic regulator of cell adhesion and the cytoskeleton plays a critical role in controlling exit from the stem cell niche and propose that Rac and Myc represent a global stem cell regulatory axis. 相似文献
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The impact of gene patents on downstream research and innovation are unknown, in part because of a lack of empirical data on the extent and nature of gene patenting. In this Policy Forum, the authors show that 20% of human gene DNA sequences are patented and that some genes are patented as many as 20 times. Unsurprisingly, genes associated with health and disease are more patented than the genome at large. The intellectual property rights for some genes can become highly fragmented between many owners, which suggests that downstream innovators may face considerable costs to gain access to gene-oriented technologies. 相似文献
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