Antigenicity of cortical bone allografts in dogs and effect of ethylene oxide-sterilization.

The aims of the present study were to determine the antigenicity of cortical bone allografts and the effect of ethylene oxide-sterilization (EO-sterilization). Cortical bone allografts from one donor dog were implanted in a muscle pouch in four groups of four dogs each. The grafts were either fresh, EO-sterilized, demineralized or demineralized and EO-sterilized. The immune response against the grafts was determined by measuring the antibody response against surface antigens of donor cells and by the mixed lymphocyte reaction. Dogs receiving EO-sterilized grafts or bone matrix did not demonstrate an immune response. Only two of the four dogs with fresh cortical bone grafts showed a very weak immune response. This suggests a priming of the host by the fresh bone grafts. However, implanting skin grafts from the donor dog subdermally, in one dog of each of the groups, four months after implanting the bone grafts did not induce a secondary immune response. Macroscopic and histologic examination of the bone grafts five months after their implantation consistently revealed graft resorption (activity of osteoclasts) and vascularization of the fresh bone grafts, but not of EO-sterilized fresh grafts. For most EO-sterilized grafts, a strong inflammatory reaction was present in the tissues surrounding the graft and this was not apparent around the non-sterilized grafts. The absence of resorption and the presence of the inflammation seemed to be unwanted effects of the EO-sterilization. The EO-sterilisation did not affect osteoinduction since osteocytes were observed in the EO-sterilized demineralized grafts. Results indicate that cortical bone allografts used in the present study are very weak antigens and that the EO-sterilization procedure used has no effect on osteoinduction, but decreases bone resorption.     

延边地区强化技术改造的对策与建议

对延边地区技术改造的投资分配、应遵循的原则提出了对策与建议．     

环模颗粒机基本参数的确定方法

通过物料的受力分析确定了物料攫取条件,并分析了物料层厚度ｈ０ 与攫取角β、模辊径比λ之间的关系,从理论上解释了颗粒机生产率随模辊径比λ和攫取角β、压辊数目Ｚ变化的规律,从而简化部件的研制过程,为正确设计颗粒机提供了科学根据     

延边工业企业技术进步及科技管理状况分析

分析了延边工业企业技术进步及科技管理状况,提出了企业管理和科技管理中普遍存在的现象．     

肉鸡血浆VLDL浓度的选择效应——对产蛋性能的影响

以零世代和一世代高、低脂系肉鸡为素材 ,个体记录高、低脂系母鸡产蛋性能 ,屠宰测定零世代 54周龄母鸡腹脂重 (率 ) ,探讨肥度性状与产蛋性能的关系 ,并研究零世代、一世代高、低脂系产蛋性能选择效应。结果表明 :在限饲条件下 ,腹脂重 (率 )与开产日龄、产蛋量表型相关很弱。腹脂重 (率 )与蛋重呈负表型相关趋势 ;零世代高、低脂系产蛋性能差异不显著。一世代低脂系比高脂系早开产 6.7d,低脂系 4 0周龄和 54周龄产蛋量显著高于高脂系 ,表明对血浆 VLDL浓度的选择使产蛋性能产生了有益的间接反应     

恒定磁场对小麦种子的生物物理机制研究

根据恒定磁场促进小麦种子三磷酸腺甙（ＡＴＰ）含量增加的作用,建立了线粒体在恒定磁场中的理论模型,并应用物理和生物化学的方法对模型进行了讨论,得出线粒体在恒定磁场下获得能量,得种子ＡＴＰ增加的根本原因。     

腾格里沙漠东缘飞播固沙植物生长状况的研究

１９９２－ １９９４ 年连续３ 年对腾格里沙漠东缘飞播区飞播植物生长状况调查结果表明：籽蒿在不同立地条件下生长状况差异很大,迎风坡生长最好,覆沙地次之,丘间地最差。在不同年份籽蒿生长状况主要受降水量的影响;三种固沙植物的生物量,沙拐枣最高为１９１．４ｇ 株－ １－ １,籽蒿次之为１８６．１ｇ 株－ １年－ １,花棒最少为５９．９ｇ株－ １－ １。     

运用致病毒素筛选抗烟草黑胫病细胞突变体I抗毒素愈伤组织的有效筛选

以６０Ｃｏ－γ射线照射烟草优质感病品种的花药作为诱变手段,用烟草黑胫病疫霉菌粗毒素为筛选剂,对烟草花粉植株叶片的愈伤组织,进行抗病突变体细胞的筛选．试验表明：毒素对烟草愈伤组织的存活和增殖有明显的抑制作用,抑制程度随毒素浓度提高而加强,同一浓度对不同品种也存在着差异．综合毒素浓度对烟草愈伤组织抑制、增殖程度及筛选的有效性,初步认为：筛选红大和Ｋ３２６抗黑胫病突变体细胞的最适粗毒素浓度分别为３０％和４５％．所筛选出的抗病突变体细胞,其细胞水平的抗性与再生植株的抗病性鉴定相吻合．     

不同激素浓度及培养基对烟草愈伤组织分化的影响

 以烟草愈伤组织为材料,研究不同激素浓度配比、不同培养基对愈伤组织绿苗分化的影响,试验结果表明,在MS+IAA0.5mg/L+KT2.0mg/L培养基上可获得较高的绿苗分化率,在分化培养基中,除必需的大量元素外,一些有机物质,维生素及微量元素也对烟草愈伤组织的分化起重要作用。