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91.
92.
Young Cheol Kim Jang Hoon Lee Yeoung-Seuk Bae Bo-Kyoon Sohn Seur Kee Park 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(4):443-450
In ginseng fields, Alternaria blight, caused by Alternaria panax, and anthracnose, caused by Colletotrichum gloeosporoides, have become serious problems in Korea, and control of these diseases relies mainly on intensive applications of fungicides.
In an effort to develop an effective environmentally-friendly control system, we utilized preventative control approaches
including rain shelter shading plates and the removal of dead plants, as well as a curative control approach, such as the
application of microbial agents. In the presence of rain shelter shading plates, the occurrence of Alternaria blight and anthracnose
decreased significantly compared to that seen with polyethylene shading nets. In addition, the eradication of dead ginseng
plants, which harboured abundant spores of the pathogens, significantly reduced the incidence of both diseases. In fields
with rain shelter shading plates and in which dead plants were eradicated, four applications of a bioformulated product containing
chitinolytic bacterial strains in a simple medium containing chitin provided control similar in effect to that observed with
the application of the fungicide under low disease pressure. The efficacy of the bioformulated product was decreased slightly
under severe disease pressure. These findings indicate that integration of the three disease management measures might constitute
a new effective and environmentally-friendly system for the control of Alternaria blight and anthracnose in Korean ginseng
fields. 相似文献
93.
Heme oxygenase-1 (HO-1) is one of the antioxidant enzymes which help protect against cellular damage. The present study examined the ability of Quercetin-3-O-β-d-glucuronopyranoside (QGC), flavonoid glucoside extracted from Rumex Aquaticus Herba, to induce expression of HO-1 and analyzed its signaling mechanism in cultured feline esophageal epithelial cells (EEC). Culture of the esophageal epithelial cells from cat was prepared. The data suggested that QGC could result in enhanced antioxidant enzyme defense system via HO-1 expression and Nrf2 translocation involving both the ERK and PI3K-Akt pathways as well as partly PKC pathways in EEC. 相似文献
94.
95.
Jung EY Lee BJ Yun YW Kang JK Baek IJ Jurg MY Lee YB Sohn HS Lee JY Kim KS Yu WJ Do JC Kim YC Nam SY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1347-1354
Genistein, a soybean-originated isoflavone, is widely consumed by humans for putative beneficial health effects but its estrogenic activity may adversely affect the development of male reproductive system. Twenty one-day-old ICR mice weaned from dams fed with a soybean-based diet throughout gestation and lactation were exposed by gavage to genistein (2.5 mg/kg b.w./day) or 17beta-estradiol (7.5 microg/kg b.w./day) for five weeks. Corn oil was used as a negative control. The animals were fed with a casein-based AIN-76A diet throughout the experimental periods. There were no significant differences in body and organ weights of mice among experimental groups. No significant differences in sperm counts and sperm motile characteristics were found between control and genistein groups. Treatment of 17beta-estradiol caused a significant decrease in prostate weight and epididymal sperm counts compared to the control (p<0.05). The levels of phospholipid hydroxide glutathione peroxidase in the testis and prostate of mice exposed to genistein or 17beta-estradiol were significantly higher than that of the control mice (p<0.05). 17beta-estradiol treatment caused degeneration and apoptosis of germ cells in the testis, depletion and degeneration in the epididymal epithelium, and hyperplasia of mucosal fold region in the prostate of mice. Genistein treatment did not cause any lesion in the testis, epididymis, and prostate. These results suggest that dietary uptake of genistein during juvenile period may not affect male reproductive development and functions. 相似文献
96.
Shin JH Sohn HJ Choi KS Kwon BJ Ko YJ An DJ Cha SH Park JH Jeong WS Park JY Choi CU Kweon CH Song JY Kim JY An SH Kim SJ Joo YS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(1):9-16
The genetic relatedness of 7 Korean type O field strains of foot-and-mouth disease virus (FMDV) in clinical specimens collected from 5 different geographic locations in 2000 was investigated. The sequence of 162 nucleotides (nt 478-639) at the 3' end of the 1D (VP1) genes was determined from amplified cDNA fragments, and subjected to the analysis for the sequence identity/divergence and phylogenetic relationship. The overall nucleotide sequence divergence among the 7 field strains was 0 to 3.8%, suggesting that they are closely related to each other. Phylogenetic analysis with the known Middle East-South Asia (ME-SA) topotype strains showed that the 7 Korean field strains formed two distinct clusters within the same lineage of the ME-SA topotype strains. Cluster 1 consisted of the strains of the primary foci of infection (Paju and Hongseong), and closely related to the strains prevailed in the Far East. Cluster 2 comprised those of subsequently affected regions (Boryeong, Yongin, and Chungju), and was further diverged from the Cluster 1. The result of phylogenetic analysis indicated that the Korean strains may have evolved from a common ancestor of the Pan Asia strains, and that at least 2 phylogenetically clustered variants within the same lineage were prevalent during the epidemic. The potential origin and sources of the virus introduction to Korea were discussed. 相似文献
97.
Sohn YS Chae C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(5):543-547
Composition of glycoconjugates were investigated in Escherichia coli 09:K103:NM, 987P+ST+-infected lower small intestines of 1-week-old pigs by the use of twenty one biotinylated-labelled lectins with avidin-biotin-peroxidase complex method. Piglets with experimental group were inoculated by feeding 5 ml of culture inoculum (5 x 10(9) colony-forming units/ml) with 15 ml of milk replacer. At the onset of diarrhea, experimental piglets and time-matched control piglets were euthanatized using electrocution, necropsied, and tested by lectin histochemistry. As compared with control, staining intensity of seven lectins altered in ileal villus brush border and goblet cells of pigs inoculated with the pathogen. 相似文献
98.
cDNA clones encoding gonadotropin (GTH) α, follicle-stimulating hormone (FSH) β and luteinzing hormone (LH) β were isolated from the pituitaries of maturing Manchurian trout (Brachymystax lenok tsinlingensis) and sequenced. The deduced amino acid sequences of GTH subunits showed high identities to masu salmon, Oncorhynchus masou: GTHα1 (95%), FSHβ (92%) and LHβ (97%), respectively. We are also attempting to produce recombinant FSH and LH using a eukaryotic expression system. In a pilot experiment, LH was secreted to the culture medium at 48 and 60 hrs after transfection. The results will be helpful to develop controlled reproduction of exterminating Manchurian trout. 相似文献
99.
Wei Ge Nga-Ling Ko Flora Yee-Man Pang Me-Fong Chung Sze-Wah Lin Chi-Wai Yuen Man-Tat Lau Lin Liu Young Chang Sohn Makito Kobayashi Katsumi Aida 《Fish physiology and biochemistry》2003,28(1-4):65-71
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play critical roles in controlling vertebrate gonadal development and function. Activin, a dimeric growth factor initially identified in the gonads, is important in the differential regulation of the two gonadotropins in mammals. Using goldfish as a model, we have demonstrated that activin stimulates FSHβ but suppresses LHβ expression. The present study demonstrated that the 5′-flanking region of goldfish FSHβ gene is functional in the mouse gonadotrope cell line, LβT2 cells. Similar to its effect on the cultured pituitary cells, activin stimulated FSHβ promoter activity in the LβT2 cells and the effect could be blocked by its binding protein follistatin. Follistatin also significantly suppressed the basal FSHβ promoter activity, suggesting secretion of endogenous activin by the LβT2 cells. Further characterization of the cis-regulatory elements responsible for activin stimulation is now under way in our laboratory. 相似文献
100.
Jihye Shin Mi Ae Kim Makito Kobayashi Young Chang Sohn 《Fish physiology and biochemistry》2013,39(5):1353-1363
Thyrotropin (thyroid-stimulating hormone, TSH), a heterodimeric glycoprotein hormone produced in the pituitary, stimulates the thyroid gland and release of thyroid hormones. In contrast to a well-known efficacy of recombinant mammalian TSHs, there is no report about the production of teleost recombinant TSH and its biological activity. In this study, we report the production of a single-chain recombinant TSH (mtTSH) of Manchurian trout (Brachymystax lenok), by baculovirus in silkworm (Bombyx mori) larvae. The mtTSH was produced in silkworm larvae and characterized as a form of N-linked glycosylation. The cAMP signaling system in transiently transfected COS-7 cells revealed that the mtTSH was recognized by their cognate receptors, salmon TSHα and TSHβ receptors, but not LH receptor. The thyrotropic potency of the mtTSH was examined by rainbow trout basibranchial tissues containing thyroid follicles. The height of follicle epithelial cells was significantly increased by treatments of mtTSH in vivo and in vitro. In conclusion, the present study suggests that the mtTSH produced by baculovirus–silkworm larvae is a biologically active recombinant TSH. 相似文献